##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920868_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3505137
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.03637889189495	31.0	30.0	33.0	24.0	34.0
2	29.08521321705828	31.0	30.0	34.0	23.0	34.0
3	29.161496968592097	31.0	30.0	34.0	23.0	34.0
4	32.67710534566837	35.0	33.0	37.0	26.0	37.0
5	32.65407029739494	35.0	33.0	37.0	26.0	37.0
6	32.776954795204865	35.0	33.0	37.0	26.0	37.0
7	32.62790099217234	35.0	33.0	37.0	25.0	37.0
8	32.576336959154524	35.0	33.0	37.0	25.0	37.0
9	33.8874329305816	37.0	33.0	39.0	25.0	39.0
10-11	33.71265103189975	37.0	33.0	39.0	25.0	39.0
12-13	33.554662200079484	37.0	33.0	39.0	23.0	39.0
14-15	34.36726538791494	38.0	33.0	40.0	23.0	41.0
16-17	34.26701010545379	38.0	33.0	40.0	22.5	41.0
18-19	34.162158141037	38.0	33.0	40.0	21.5	41.0
20-21	34.082830000653324	38.0	33.0	40.0	19.0	41.0
22-23	33.87516821739065	38.0	33.0	40.0	18.5	41.0
24-25	33.69322269001184	38.0	32.0	40.0	18.0	41.0
26-27	33.74290163266086	38.0	33.0	40.0	18.0	41.0
28-29	33.59743813151954	38.0	32.5	40.0	17.5	41.0
30-31	33.36454238450594	38.0	32.0	40.0	16.0	41.0
32-33	33.137511743478214	38.0	32.0	40.0	15.5	41.0
34-35	32.81874445991697	38.0	31.5	40.0	12.0	41.0
36-37	32.53001794223735	37.5	31.0	40.0	9.0	41.0
38-39	32.34071835137971	37.0	31.0	40.0	8.5	41.0
40-41	32.152149545081976	37.0	30.0	40.0	8.0	41.0
42-43	31.930296447756536	37.0	30.0	40.0	7.5	41.0
44-45	31.672069451208326	37.0	30.0	40.0	2.0	41.0
46-47	31.43063067149729	37.0	30.0	40.0	2.0	41.0
48-49	31.140601636968825	36.0	29.5	39.5	2.0	40.5
50-51	29.978767449032663	34.5	27.5	38.0	2.0	39.5
52-53	30.43634528407877	35.5	28.5	38.5	2.0	39.5
54-55	31.115653682010148	36.0	30.0	39.0	2.0	40.5
56-57	31.082199069537083	36.0	30.0	39.5	2.0	41.0
58-59	30.85429713588941	36.0	29.0	39.0	2.0	41.0
60-61	30.53928348592366	35.5	29.0	39.0	2.0	40.5
62-63	30.16218082203349	35.0	28.0	39.0	2.0	40.0
64-65	29.750341142157925	35.0	28.0	38.5	2.0	40.0
66-67	29.37247944944805	34.5	28.0	38.0	2.0	40.0
68-69	28.933671066209392	34.0	27.0	37.5	2.0	40.0
70-71	28.611276249687243	34.0	26.0	37.0	2.0	39.5
72-73	28.14330666675796	34.0	26.0	36.0	2.0	39.0
74-75	27.699748968442606	34.0	26.0	36.0	2.0	39.0
76-77	27.28116433109462	33.0	25.5	35.5	2.0	38.0
78-79	26.823151420329648	33.0	24.5	35.0	2.0	37.0
80-81	26.377102521242392	33.0	23.5	35.0	2.0	37.0
82-83	25.902598243663515	32.0	21.5	35.0	2.0	36.0
84-85	25.509679507534226	32.0	20.0	35.0	2.0	36.0
86-87	25.064742262570622	32.0	18.5	35.0	2.0	35.0
88-89	24.544167888444875	31.5	14.0	34.0	2.0	35.0
90-91	24.063558143376422	31.0	4.5	34.0	2.0	35.0
92-93	23.53665106385285	31.0	2.0	34.0	2.0	35.0
94-95	22.912394294431287	31.0	2.0	34.0	2.0	35.0
96-97	22.28329848448149	30.0	2.0	34.0	2.0	35.0
98	21.895515353608147	30.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	149678.0
3	25827.0
4	18878.0
5	15766.0
6	14636.0
7	14532.0
8	17082.0
9	19493.0
10	21340.0
11	23382.0
12	24769.0
13	24970.0
14	23628.0
15	24575.0
16	26762.0
17	28842.0
18	29713.0
19	29160.0
20	30274.0
21	31852.0
22	35354.0
23	39983.0
24	45504.0
25	52622.0
26	60932.0
27	71398.0
28	83023.0
29	97525.0
30	113383.0
31	135054.0
32	162856.0
33	199475.0
34	251152.0
35	321811.0
36	416849.0
37	479081.0
38	308587.0
39	35386.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.2627645256436	17.912889092523123	12.83920849918387	24.985137882649408
2	16.13584772134181	19.571677037066628	39.089107441719115	25.203367799872446
3	19.44278864769899	23.28025811215963	28.87365841228607	28.40329482785532
4	13.325604441478184	15.362955933046834	36.155634353602224	35.15580527187276
5	14.602343271362969	36.674716507767194	34.02802805716329	14.694912163706544
6	34.129331796182996	34.52883869053054	17.104516710802667	14.237312802483792
7	29.93635607499169	31.098810011256457	20.632187761705502	18.33264615204635
8	26.810929287292097	34.43859922427671	19.82902017713699	18.92145131129421
9	26.89392739855817	15.677161834187936	19.38608961646863	38.04282115078526
10-11	25.90836703625765	25.218579250562602	27.357697390343773	21.515356322835977
12-13	26.451924810725803	22.46405673318558	27.398466976018042	23.685551480070576
14-15	23.504469558727365	24.060268572948015	24.945093413948584	27.490168454376036
16-17	23.897385463154595	27.145299408663536	25.475913724107276	23.48140140407459
18-19	23.734232985294703	26.365977538858065	26.91505558063098	22.98473389521625
20-21	24.338599941669827	25.828774533897526	26.604868470885805	23.227757053546842
22-23	24.105490783727614	25.54121461039216	26.526244548374457	23.82705005750577
24-25	23.851705076741407	25.667994731892165	26.70024868928912	23.780051502077313
26-27	23.726885473973137	25.869754546605627	26.472385618792448	23.930974360628788
28-29	23.728177339043715	26.121382086517496	26.528708451861515	23.621732122577278
30-31	23.95374194708554	25.85353043766181	26.360938976248722	23.831788639003936
32-33	23.843270853268987	25.82466365787232	26.31868499526823	24.013380493590464
34-35	24.05276607876127	25.898058933274864	26.266384745334815	23.782790242629048
36-37	24.163945420916516	25.652493249872084	26.290466896810678	23.89309443240073
38-39	24.005501141557094	25.761085829157942	26.40153862292045	23.831874406364516
40-41	24.135615723538056	25.637534953226343	26.34867393790275	23.87817538533285
42-43	24.051371996028035	25.613056380502066	26.247399341405238	24.088172282064665
44-45	23.86370129690651	25.66496908857371	26.324771832300232	24.146557782219553
46-47	23.85961088344032	25.713482515303333	26.216332951060263	24.210573650196082
48-49	23.961096564804336	25.70619515153338	26.128980753768378	24.203727529893914
50-51	23.760005235033084	25.823375768698597	26.283674930219846	24.132944066048477
52-53	23.886898445754127	25.669399733102637	26.31449243509027	24.129209386052967
54-55	23.591428165748567	25.497176501974433	26.51897431077434	24.39242102150266
56-57	23.522304760829815	25.60941652483974	26.532525753010024	24.335752961320427
58-59	23.575060078424183	25.432478725506762	26.579210430588297	24.413250765480758
60-61	23.64715204378157	25.384483829420557	26.64092077882791	24.327443347969968
62-63	23.474969682700895	25.461475978445357	26.659388796376014	24.404165542477728
64-65	23.310065190916255	25.31159825202378	26.636621534493877	24.741715022566087
66-67	23.392573219883563	25.422961038961038	26.48475055978504	24.699715181370355
68-69	23.39797996579806	25.12630942730369	26.67171293285936	24.803997674038893
70-71	23.3579076141616	25.01232293167663	26.585277335755684	25.044492118406087
72-73	23.40123342715564	25.142483410514977	26.523922969993418	24.932360192335963
74-75	23.28263459410381	25.119233061348567	26.59685857879801	25.00127376574961
76-77	23.311012626741856	25.002855207081943	26.68417860343592	25.001953562740276
78-79	23.299328076427173	25.04502126944595	26.573060972602537	25.082589681524343
80-81	23.3272231906396	24.9793664594104	26.64755565187245	25.04585469807755
82-83	23.350308008918933	25.021634624330456	26.55488037906324	25.073176987687372
84-85	23.277945640956645	24.88326789770572	26.549350104701773	25.289436356635857
86-87	23.284628013825902	24.815526777669266	26.676602555662544	25.22324265284229
88-89	23.352469949242007	24.601862176276782	26.720448489200972	25.32521938528024
90-91	23.277001541656944	24.66663994652154	26.669906484263507	25.386452027558015
92-93	23.395370606269665	24.501635974843488	26.735493068218574	25.367500350668276
94-95	23.310470514492867	24.374058943101943	26.76843548266351	25.54703505974168
96-97	23.506001644105663	24.11166978640225	26.76987917125967	25.612449398232425
98	23.359505518602024	24.10871628809175	26.685266208567544	25.84651198473868
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	70.0
1	66.5
2	105.0
3	174.0
4	184.0
5	193.5
6	305.0
7	587.5
8	904.0
9	1084.5
10	1115.0
11	1175.5
12	1319.0
13	1469.0
14	1710.5
15	1874.0
16	1947.0
17	2042.0
18	2092.5
19	2138.5
20	2177.0
21	2287.0
22	2534.0
23	2922.0
24	3414.5
25	4242.5
26	5531.0
27	7520.0
28	10247.5
29	14090.5
30	19595.0
31	26660.5
32	33842.0
33	41045.0
34	48709.0
35	57214.5
36	67346.5
37	76988.5
38	84308.0
39	92654.5
40	102050.0
41	110116.0
42	118957.5
43	127521.0
44	136135.0
45	142137.5
46	145919.5
47	151470.0
48	154449.0
49	153912.5
50	153309.0
51	153336.5
52	151934.0
53	150022.0
54	146222.5
55	138241.5
56	127395.0
57	115629.5
58	103904.0
59	89612.0
60	74888.0
61	62063.0
62	51562.0
63	41365.5
64	31219.0
65	23530.5
66	18242.5
67	14608.0
68	10868.5
69	8072.5
70	6482.5
71	5727.0
72	4823.0
73	3308.0
74	2148.0
75	1334.5
76	801.0
77	476.0
78	286.5
79	157.0
80	102.5
81	81.5
82	50.0
83	28.0
84	17.0
85	15.5
86	12.0
87	14.0
88	13.5
89	3.5
90	2.0
91	3.0
92	3.5
93	3.5
94	3.5
95	3.5
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.37193981290888206
2	0.022024816719004137
3	0.017517146975995518
4	0.015348900770497702
5	0.020541279841558263
6	0.023308646709101526
7	0.013751245671709836
8	0.007959745938603826
9	0.0
10-11	2.8529555335497586E-5
12-13	0.004821494851699092
14-15	0.0026532486462012756
16-17	0.005563263290422029
18-19	0.0360470931664012
20-21	0.07593141152542682
22-23	0.09456121115950675
24-25	0.06195192941103301
26-27	0.07122403489506972
28-29	0.0549621883538361
30-31	0.049841133171114285
32-33	0.018044943749702225
34-35	0.32783312036020273
36-37	0.5024197342357802
38-39	0.45769965624738773
40-41	0.46695749695375677
42-43	0.41867122454842703
44-45	0.3934368328541794
46-47	0.3920246198650723
48-49	0.38270972004803233
50-51	0.3788011709670692
52-53	0.4395263294986758
54-55	0.43951206472100807
56-57	0.4102550057244553
58-59	0.39561934383734504
60-61	0.40948470773039686
62-63	0.3917393243117174
64-65	0.43898426794730133
66-67	0.45859833724045596
68-69	0.4024664371178644
70-71	0.3613125535464091
72-73	0.34767542609604135
74-75	0.32977313012301657
76-77	0.3286604774649322
78-79	0.32838944668924497
80-81	0.3434387871287199
82-83	0.3393305311604083
84-85	0.3389881764963823
86-87	0.32537957860135
88-89	0.36743214316587336
90-91	0.3464058608836117
92-93	0.33684845984622
94-95	0.3439665839024266
96-97	0.3438524656810847
98	0.33838905583433687
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3505137.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.51221423987053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98856523447898	36.868140441847686
2	12.72514947179683	12.855509527075535
3	5.304196187182321	8.037800825317735
4	2.7401114086575915	5.536363780609027
5	1.6933360815597296	4.276707746592396
6	1.061723570948326	3.217800508755733
7	0.6947684291854455	2.456600421947948
8	0.5122458677895202	2.069973841381811
9	0.37162129717584313	1.6894273121140304
>10	1.7611767582980786	15.585764144470538
>50	0.10534092553168564	3.624717386670231
>100	0.04057086755174827	3.275940256680758
>500	9.664903508665825E-4	0.31637951554298405
>1k	2.2740949302169666E-4	0.18887429099360037
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.8529555335497586E-5	0.0	0.0	0.0	0.0
3	2.8529555335497586E-5	0.0	0.0	2.8529555335497586E-5	0.0
4	2.8529555335497586E-5	0.0	0.0	2.8529555335497586E-5	0.0
5	2.8529555335497586E-5	2.8529555335497586E-5	0.0	2.8529555335497586E-5	0.0
6	8.558866600649276E-5	2.8529555335497586E-5	0.0	2.8529555335497586E-5	0.0
7	1.7117733201298552E-4	2.8529555335497586E-5	0.0	2.8529555335497586E-5	0.0
8	1.7117733201298552E-4	2.8529555335497586E-5	0.0	2.8529555335497586E-5	0.0
9	1.7117733201298552E-4	2.8529555335497586E-5	0.0	2.8529555335497586E-5	0.0
10-11	1.7117733201298552E-4	2.8529555335497586E-5	0.0	2.8529555335497586E-5	0.0
12-13	2.5676599801947826E-4	2.8529555335497586E-5	0.0	2.8529555335497586E-5	0.0
14-15	3.1382510869047345E-4	2.8529555335497586E-5	0.0	2.8529555335497586E-5	0.0
16-17	3.1382510869047345E-4	2.8529555335497586E-5	0.0	2.8529555335497586E-5	0.0
18-19	3.851489970292174E-4	2.8529555335497586E-5	0.0	9.985344367424155E-5	0.0
20-21	4.279433300324638E-4	2.8529555335497586E-5	0.0	1.7117733201298552E-4	0.0
22-23	4.564728853679614E-4	2.8529555335497586E-5	0.0	2.995603310227247E-4	0.0
24-25	5.705911067099517E-4	2.8529555335497586E-5	0.0	5.991206620454494E-4	0.0
26-27	5.705911067099517E-4	2.8529555335497586E-5	0.0	0.0011982413240908987	0.0
28-29	7.56033216390686E-4	2.8529555335497586E-5	0.0	0.006989741057196909	0.0
30-31	8.558866600649276E-4	2.8529555335497586E-5	0.0	0.026974694569712965	0.0
32-33	8.701514377326763E-4	2.8529555335497586E-5	0.0	0.07026829479133055	0.0
34-35	0.001027063992077913	2.8529555335497586E-5	0.0	0.13069389299191442	0.0
36-37	0.0012125061017586474	2.8529555335497586E-5	0.0	0.22682422969487356	0.0
38-39	0.0013123595454328889	2.8529555335497586E-5	0.0	0.38502061403020765	0.0
40-41	0.0013123595454328889	2.8529555335497586E-5	0.0	0.5660406426339398	0.0
42-43	0.001383683433771633	2.8529555335497586E-5	0.0	0.7605694156890301	0.0
44-45	0.0014264777667748793	2.8529555335497586E-5	0.0	0.9936273532247099	0.0
46-47	0.001597655098787865	2.8529555335497586E-5	0.0	1.2757989202704487	0.0
48-49	0.0016832437647943575	2.8529555335497586E-5	0.0	1.5784975023800782	0.0
50-51	0.0016832437647943575	2.8529555335497586E-5	0.0	1.9081137199487497	0.0
52-53	0.0016832437647943575	2.8529555335497586E-5	0.0	2.2409965716033353	0.0
54-55	0.0017117733201298551	7.132388833874396E-5	0.0	2.619555241350053	0.0
56-57	0.0017688324308008502	8.558866600649276E-5	0.0	3.0666276382349675	0.0
58-59	0.0017688324308008502	1.1411822134199034E-4	0.0	3.5531136158158727	0.0
60-61	0.0017830972084685991	1.2838299900973913E-4	0.0	4.064135010985305	0.0
62-63	0.0017973619861363479	1.4264777667748793E-4	0.0	4.588822063160441	0.0
64-65	0.0017973619861363479	1.4264777667748793E-4	0.0	5.161752593407904	0.0
66-67	0.0018829506521428406	1.4264777667748793E-4	0.0	5.80081463292305	0.0
68-69	0.0019400097628138359	1.4264777667748793E-4	0.0	6.500259476305777	0.0
70-71	0.0020113336511525795	1.4264777667748793E-4	0.0	7.241200557923984	0.0
72-73	0.002082657539491324	1.4264777667748793E-4	0.0	7.996834360539973	1.4264777667748793E-5
74-75	0.0021111870948268214	1.4264777667748793E-4	0.0	8.778101968624906	2.8529555335497586E-5
76-77	0.0021111870948268214	1.4264777667748793E-4	0.0	9.629694930611842	2.8529555335497586E-5
78-79	0.0021111870948268214	1.4264777667748793E-4	0.0	10.546306749208377	2.8529555335497586E-5
80-81	0.002353688315178551	1.4264777667748793E-4	0.0	11.494557844671977	2.8529555335497586E-5
82-83	0.0023679530928462996	1.4264777667748793E-4	0.0	12.463435808643144	5.705911067099517E-5
84-85	0.0024535417588527923	1.4264777667748793E-4	0.0	13.449160475039921	5.705911067099517E-5
86	0.002539130424859285	1.4264777667748793E-4	0.0	14.208802680180547	5.705911067099517E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2865	0.0	43.213406	1
GTATCAA	5135	0.0	41.67766	1
ATCAACG	6470	0.0	32.323452	3
TCAACGC	6540	0.0	31.767103	4
CAACGCA	6550	0.0	31.578566	5
TATCAAC	6865	0.0	30.59854	2
AACGCAG	6950	0.0	30.020765	6
GTACATG	10275	0.0	25.531942	1
ACGCAGA	8200	0.0	25.33042	7
TACATGG	10315	0.0	24.94419	2
CGCAGAG	8630	0.0	23.90686	8
ACATGGG	10655	0.0	23.372412	3
GCAGAGT	9680	0.0	20.508766	9
CATGGGG	7335	0.0	19.883102	4
GAGTACA	7815	0.0	18.669003	1
GTATAGG	875	0.0	18.409874	1
AGAGTAC	8665	0.0	17.938112	10-11
AGTACAT	7670	0.0	17.281374	2
GTGTAGC	2050	0.0	15.940256	1
CAGAGTA	9520	0.0	15.893613	10-11
>>END_MODULE