FastQCFastQC Report
Mon 15 Aug 2016
SRR2920868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3505137
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30900.044.1693231
GTATCAA55950.043.3478361
TCAACGC70250.033.584374
ATCAACG70950.033.3868373
CAACGCA72400.032.581465
TATCAAC74750.032.131832
AACGCAG77400.030.6606776
ACGCAGA91500.025.4690697
GTACATG106150.025.2671591
TACATGG107600.024.2631152
CGCAGAG95150.024.0400338
ACATGGG113200.022.3935013
GCAGAGT108000.020.2569489
CATGGGG74650.019.1409654
GAGTACA82450.018.5721651
AGAGTAC91850.018.02990510-11
GTGGTAT20650.017.9627111
AGTACAT84650.017.3832452
ATACCGT3059.374162E-817.1177046
GTATTAC5650.016.8336731