FastQCFastQC Report
Mon 15 Aug 2016
SRR2920867_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920867_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3616703
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29300.040.997531
GTATCAA52350.040.089721
TCAACGC64250.031.839954
ATCAACG65850.030.9270023
CAACGCA66450.030.5096975
TATCAAC69050.029.627012
AACGCAG71650.028.6115846
GTACATG102000.023.3731
ACGCAGA84750.023.2104787
TACATGG108650.021.8261662
CGCAGAG88850.021.7256688
ACATGGG109150.021.0114923
GTGTAGC22850.020.1418191
CATGGGG74650.018.7404794
GCAGAGT103150.017.9139069
GAGTACA81200.017.5707591
AGAGTAC84100.017.44674710-11
GTATATA14250.017.4406931
AGTACAT77250.017.0411952
GTAGCCC18600.017.015583
Produced by FastQC (version 0.11.5)