##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920867_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3616703
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.372383355780112	31.0	28.0	31.0	18.0	34.0
2	28.409698280450453	31.0	28.0	33.0	16.0	34.0
3	28.482543908084242	31.0	28.0	34.0	16.0	34.0
4	31.9312174651886	35.0	32.0	37.0	20.0	37.0
5	31.889411433562557	35.0	32.0	37.0	20.0	37.0
6	32.003879500196724	35.0	32.0	37.0	22.0	37.0
7	31.841155881475476	35.0	32.0	37.0	19.0	37.0
8	31.779594287946786	35.0	32.0	37.0	19.0	37.0
9	33.02771916853554	37.0	32.0	39.0	18.0	39.0
10-11	32.82579700351397	37.0	32.0	39.0	17.0	39.0
12-13	32.654902130476295	37.0	32.0	39.0	17.0	39.0
14-15	33.386738557188686	38.0	32.0	40.0	17.0	41.0
16-17	33.277013069638286	38.0	32.0	40.0	16.5	41.0
18-19	33.16522340927635	38.0	32.0	40.0	15.5	41.0
20-21	33.070946522288395	38.0	32.0	40.0	11.5	41.0
22-23	32.83677440475483	38.0	32.0	40.0	10.0	41.0
24-25	32.64958444196275	38.0	31.0	40.0	10.0	41.0
26-27	32.689383673472776	38.0	31.0	40.0	10.0	41.0
28-29	32.530236516517945	38.0	31.0	40.0	9.0	41.0
30-31	32.285260774799596	37.0	30.5	40.0	8.5	41.0
32-33	32.04949341983569	37.0	30.0	40.0	8.0	41.0
34-35	31.723597016398635	37.0	30.0	40.0	4.5	41.0
36-37	31.41974099062046	37.0	30.0	40.0	2.0	41.0
38-39	31.225711511285276	37.0	30.0	40.0	2.0	41.0
40-41	31.036872809296202	36.5	29.5	40.0	2.0	41.0
42-43	30.81497125973573	36.0	29.0	39.5	2.0	41.0
44-45	30.54313915187396	36.0	28.5	39.0	2.0	40.5
46-47	30.296727433798132	36.0	28.0	39.0	2.0	40.0
48-49	30.006009340551323	35.0	27.0	39.0	2.0	40.0
50-51	28.90007902224761	34.0	25.5	38.0	2.0	39.5
52-53	29.35692742810234	34.5	27.0	38.5	2.0	39.5
54-55	30.009601977270457	36.0	28.0	39.0	2.0	40.0
56-57	29.965017033469437	35.5	28.0	39.0	2.0	41.0
58-59	29.727787020388458	35.0	27.5	39.0	2.0	40.5
60-61	29.409083079257545	35.0	27.0	39.0	2.0	40.0
62-63	29.024280677733284	35.0	26.0	39.0	2.0	40.0
64-65	28.60896581776275	34.0	26.0	38.0	2.0	40.0
66-67	28.220124240226525	34.0	25.5	38.0	2.0	40.0
68-69	27.76819882638967	34.0	24.5	37.0	2.0	40.0
70-71	27.43764265409684	34.0	24.0	37.0	2.0	39.0
72-73	26.967670002209196	33.0	22.5	36.0	2.0	39.0
74-75	26.517333742914474	33.0	22.0	36.0	2.0	39.0
76-77	26.08023882525051	32.5	20.5	35.0	2.0	37.5
78-79	25.5998412089685	32.0	19.0	35.0	2.0	37.0
80-81	25.132087014056726	32.0	17.5	35.0	2.0	36.5
82-83	24.636602590812682	31.5	10.0	35.0	2.0	36.0
84-85	24.222200164072085	31.0	7.0	34.5	2.0	35.5
86-87	23.750847387800434	31.0	2.0	34.0	2.0	35.0
88-89	23.208094084584772	31.0	2.0	34.0	2.0	35.0
90-91	22.697548568406088	30.0	2.0	34.0	2.0	35.0
92-93	22.1403267008654	30.0	2.0	34.0	2.0	35.0
94-95	21.49376780454464	29.5	2.0	34.0	2.0	35.0
96-97	20.84113749456342	29.0	2.0	34.0	2.0	35.0
98	20.441199899466447	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	201076.0
3	33950.0
4	24287.0
5	19631.0
6	18134.0
7	18352.0
8	20744.0
9	23946.0
10	26289.0
11	28272.0
12	28922.0
13	28944.0
14	27511.0
15	28694.0
16	30683.0
17	33363.0
18	34220.0
19	34488.0
20	35930.0
21	37644.0
22	41246.0
23	46167.0
24	52455.0
25	59614.0
26	68435.0
27	79210.0
28	91589.0
29	104911.0
30	122033.0
31	142374.0
32	168249.0
33	202531.0
34	249560.0
35	314326.0
36	396883.0
37	443359.0
38	271826.0
39	26853.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.475156106072475	18.38486727896271	13.386891891254313	24.7530847237105
2	15.766031242333991	19.665722569202547	39.59313003847836	24.97511614998511
3	19.384463325349373	23.70600766205964	28.987867529830623	27.921661482760367
4	13.225952304903494	15.95121256493538	36.97496885452401	33.84786627563712
5	14.43724781450763	36.84603237377243	34.32283614822354	14.393883663496402
6	33.20164650480019	35.17183264369444	17.494860203622775	14.131660647882601
7	28.96967229956095	31.33010655663585	21.12475004832422	18.57547109547898
8	26.33189017866747	34.53696690864878	20.281590749652345	18.849552163031404
9	26.569143222432146	15.895250453244294	19.70438269329829	37.831223631025274
10-11	25.36131334034617	25.466768545869765	27.96790113864802	21.204016975136046
12-13	26.03264068945666	22.710021127993166	27.72387557240658	23.533462610143594
14-15	23.247869297856447	24.367515306893413	25.229432314615746	27.155183080634394
16-17	23.376203404996364	27.30909835977181	25.899161318276825	23.415536916954995
18-19	23.32613824037935	26.644542954400684	27.24491463940497	22.78440416581499
20-21	24.28468790991168	25.917119551119	26.870158796523874	22.92803374244545
22-23	23.808806877189234	25.76165939049536	26.67506808567344	23.75446564664196
24-25	23.58266258049933	25.870203412892234	26.81691179705135	23.730222209557084
26-27	23.477332382983967	26.147727400069094	26.682160570951158	23.692779645995785
28-29	23.528413389177594	26.258366714430025	26.65124904711891	23.56197084927347
30-31	23.88010981192329	26.06897602926547	26.45769961503861	23.59321454377262
32-33	23.48429235357992	26.066339963587094	26.40823467644241	24.04113300639058
34-35	23.944469789156805	26.10477786126352	26.40998707119139	23.540765278388278
36-37	24.02305834770184	25.820269442020166	26.348484960065292	23.808187250212697
38-39	23.76383991386785	25.955490334230124	26.444039172239158	23.836630579662863
40-41	23.963055613816017	25.80717747278619	26.506701187726318	23.723065725671475
42-43	23.872988300932953	25.823480682427295	26.328835842246335	23.97469517439341
44-45	23.765956481144386	25.837136221515816	26.498969927261307	23.89793737007849
46-47	23.75095525187976	25.761154968572804	26.374792398401866	24.11309738114557
48-49	23.676377829820453	25.793265677855842	26.43964957932171	24.090706913001995
50-51	23.633103953223056	25.899733039797844	26.475341256515627	23.991821750463473
52-53	23.63791808005981	25.798911664716417	26.53238338317614	24.030786872047635
54-55	23.466207172546216	25.704921847998406	26.713580686805592	24.11529029264978
56-57	23.455061054042567	25.81076620109506	26.621580988886418	24.112591755975956
58-59	23.41743549029596	25.678172533877053	26.6697988682435	24.234593107583493
60-61	23.398301777806836	25.61249844002715	26.842069389535943	24.14713039263007
62-63	23.309138396638645	25.64494886918731	26.87188295937058	24.174029774803465
64-65	23.240968750581466	25.441852831160162	26.765710315171365	24.551468103087007
66-67	23.1824527700217	25.53695177058947	26.709340219175097	24.57125524021373
68-69	23.3094624462794	25.391783345569298	26.67242999300544	24.62632421514586
70-71	23.07919536875972	25.18773170319181	26.78787555879675	24.945197369251726
72-73	23.036565177117108	25.14878547298305	26.842509992443325	24.97213935745652
74-75	22.975263309084287	25.281417153744375	26.790635624446825	24.952683912724517
76-77	22.967225047755427	25.07642532538325	26.87935561938357	25.076994007477754
78-79	22.85477142509988	25.063318107397496	26.93513451734264	25.146775950159984
80-81	22.92018103042625	24.943400246819316	26.90732844725791	25.229090275496524
82-83	22.892969668906378	24.834927610074732	27.004440540577658	25.26766218044123
84-85	22.807578259557165	24.896085801416927	26.96654603686145	25.329789902164457
86-87	22.76320092806575	24.777103051193688	27.094992755727805	25.36470326501276
88-89	22.744818726722414	24.610217252636442	27.07724444137326	25.567719579267884
90-91	22.705398252592126	24.595725164279898	27.110167936110113	25.588708647017867
92-93	22.76196218990441	24.353397483460668	27.170712496984684	25.713927829650242
94-95	22.614000990498702	24.305506423675418	27.273992195314133	25.806500390511744
96-97	22.742761969790756	24.112099539961175	27.237580741239327	25.907557749008742
98	22.729632185113452	23.935447553842486	27.15710501105717	26.177815249986892
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	72.0
1	54.0
2	82.0
3	134.5
4	133.0
5	165.5
6	262.5
7	463.0
8	710.5
9	842.5
10	926.0
11	1071.0
12	1236.5
13	1397.5
14	1591.0
15	1736.0
16	1842.5
17	1867.0
18	1835.0
19	1830.0
20	1875.5
21	1996.5
22	2145.5
23	2365.0
24	2775.5
25	3512.0
26	4613.5
27	6160.0
28	8445.0
29	11933.5
30	17195.0
31	24571.5
32	32806.5
33	41487.0
34	51547.5
35	62904.0
36	73965.5
37	84228.0
38	94330.0
39	104794.5
40	114402.5
41	121930.5
42	130287.5
43	139337.5
44	146269.5
45	152864.5
46	159884.0
47	165186.0
48	168473.0
49	167963.0
50	166247.0
51	164116.0
52	159553.0
53	155507.5
54	148415.5
55	138465.0
56	125787.0
57	111270.0
58	98934.5
59	85562.0
60	71563.5
61	58916.0
62	47383.5
63	36010.5
64	26213.0
65	19402.5
66	14467.0
67	10761.5
68	7679.0
69	5342.5
70	3687.0
71	2648.0
72	2033.5
73	1438.0
74	1052.0
75	750.0
76	464.5
77	294.0
78	188.0
79	97.5
80	65.5
81	41.5
82	22.0
83	17.0
84	14.0
85	11.0
86	10.5
87	9.0
88	6.5
89	7.0
90	7.5
91	5.0
92	2.0
93	5.5
94	5.5
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.374484717158141
2	0.02499514060181331
3	0.019465242238580274
4	0.01681089102422842
5	0.02247903684654228
6	0.022147242944748298
7	0.013935343875347244
8	0.008847837381172853
9	0.0
10-11	4.147423772424775E-5
12-13	0.004811011576012739
14-15	0.0031382173211347464
16-17	0.005364001412336042
18-19	0.03528075155742675
20-21	0.0757181333385683
22-23	0.09468568472445761
24-25	0.06171366573368065
26-27	0.07170895702522435
28-29	0.05550635482095157
30-31	0.04963083781001647
32-33	0.0187325307054519
34-35	0.3307570458508758
36-37	0.505059442259981
38-39	0.4604608119605066
40-41	0.4691565771366905
42-43	0.4196225125480306
44-45	0.39504211432345976
46-47	0.3940052583803536
48-49	0.38406526607244224
50-51	0.37966899687367195
52-53	0.4391430537702432
54-55	0.4404978788692353
56-57	0.41181153110996394
58-59	0.3964384136601762
60-61	0.4113553144949972
62-63	0.3933831448144898
64-65	0.4396269198770261
66-67	0.4606128841654955
68-69	0.4041387971309781
70-71	0.3619180231276939
72-73	0.3485080195968538
74-75	0.3305911488999788
76-77	0.32839301430059364
78-79	0.32872480820238764
80-81	0.34415322463580783
82-83	0.34007492459292343
84-85	0.3397293059452214
86-87	0.32587691054532264
88-89	0.36822210726177956
90-91	0.3474020399242072
92-93	0.33703348049314524
94-95	0.3444573690457856
96-97	0.34401497717672697
98	0.33881687271528793
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3616703.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.93095268167231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29344207309704	39.52785156321086
2	12.381583827569576	13.355012230576277
3	5.454967776392893	8.82574827086077
4	2.8437628767861796	6.13467364583407
5	1.7089214067354443	4.608187976167306
6	1.0529760242932171	3.4072800084655754
7	0.7258526462702035	2.740214730390548
8	0.5016554833520427	2.164380650812837
9	0.38783359855377697	1.8824611906769793
>10	1.5861188079655721	14.092157290703868
>50	0.04883043563690422	1.7786132171024618
>100	0.013436261958825819	1.180434091184091
>500	4.1252092892390496E-4	0.1516733305943875
>1k	2.0626045943907396E-4	0.15131180341997327
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1059796726466066E-4
2	0.0	0.0	0.0	2.7649491816165165E-5	1.3824745908082582E-4
3	0.0	0.0	0.0	5.529898363233033E-5	1.3824745908082582E-4
4	0.0	0.0	0.0	5.529898363233033E-5	1.3824745908082582E-4
5	0.0	0.0	0.0	5.529898363233033E-5	1.3824745908082582E-4
6	0.0	0.0	0.0	5.529898363233033E-5	3.0414440997781684E-4
7	0.0	0.0	0.0	5.529898363233033E-5	3.0414440997781684E-4
8	0.0	0.0	0.0	5.529898363233033E-5	3.0414440997781684E-4
9	0.0	0.0	0.0	8.29484754484955E-5	5.529898363233033E-4
10-11	0.0	0.0	0.0	8.29484754484955E-5	6.221135658637162E-4
12-13	0.0	0.0	0.0	8.29484754484955E-5	6.912372954041291E-4
14-15	0.0	0.0	0.0	8.29484754484955E-5	6.912372954041291E-4
16-17	0.0	0.0	0.0	8.29484754484955E-5	6.912372954041291E-4
18-19	0.0	0.0	0.0	9.677322135657808E-5	6.912372954041291E-4
20-21	0.0	0.0	0.0	1.2442271317274325E-4	7.188867872202943E-4
22-23	0.0	0.0	0.0	2.9031966406973424E-4	7.465362790364594E-4
24-25	0.0	0.0	0.0	6.91237295404129E-4	7.465362790364594E-4
26-27	0.0	0.0	0.0	0.0016174952712456623	7.741857708526246E-4
28-29	0.0	0.0	0.0	0.005336351920519877	7.741857708526246E-4
30-31	0.0	0.0	0.0	0.022354614133369536	7.741857708526246E-4
32-33	0.0	0.0	0.0	0.060414139618320886	0.0010230311971981111
34-35	0.0	0.0	0.0	0.1127131533885973	0.0011474539103708543
36-37	0.0	0.0	0.0	0.20221455839752395	0.0012995261153597626
38-39	0.0	0.0	0.0	0.35083057690941166	0.001354825098992093
40-41	0.0	0.0	0.0	0.5264739736716009	0.001354825098992093
42-43	0.0	0.0	0.0	0.7107163623886175	0.001354825098992093
44-45	0.0	0.0	0.0	0.9391149895360498	0.0013824745908082583
46-47	0.0	0.0	0.0	1.211669855113898	0.0013824745908082583
48-49	0.0	0.0	0.0	1.505459530406561	0.0013962993367163407
50-51	0.0	0.0	0.0	1.8202904689713255	0.0014101240826244234
52-53	0.0	0.0	0.0	2.1408310275961284	0.0014101240826244234
54-55	0.0	0.0	0.0	2.5081683511197905	0.001423948828532506
56-57	0.0	0.0	0.0	2.9332378135556056	0.0014515983203486713
58-59	0.0	0.0	0.0	3.393007388220708	0.0014930725580729188
60-61	0.0	0.0	0.0	3.8787398357011895	0.0014930725580729188
62-63	0.0	0.0	0.0	4.37587769855584	0.0014930725580729188
64-65	0.0	0.0	0.0	4.907259457024809	0.0015068973039810015
66-67	0.0	0.0	0.0	5.515327081045914	0.0015207220498890841
68-69	1.3824745908082582E-5	0.0	0.0	6.183117607389935	0.0015483715417052492
70-71	2.7649491816165165E-5	0.0	0.0	6.897580475919643	0.001589845779429497
72-73	2.7649491816165165E-5	0.0	0.0	7.617932686206194	0.0016036705253375796
74-75	2.7649491816165165E-5	0.0	0.0	8.359547355699377	0.0016036705253375796
76-77	2.7649491816165165E-5	0.0	0.0	9.16669132079687	0.0016313200171537447
78-79	2.7649491816165165E-5	0.0	0.0	10.048378315830744	0.0016313200171537447
80-81	2.7649491816165165E-5	0.0	0.0	10.969534960432195	0.0016313200171537447
82-83	2.7649491816165165E-5	0.0	0.0	11.900133906488865	0.0016451447630618273
84-85	2.7649491816165165E-5	2.7649491816165165E-5	0.0	12.836898689220542	0.00165896950896991
86	2.7649491816165165E-5	2.7649491816165165E-5	0.0	13.55864719884381	0.00165896950896991
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2930	0.0	40.99753	1
GTATCAA	5235	0.0	40.08972	1
TCAACGC	6425	0.0	31.83995	4
ATCAACG	6585	0.0	30.927002	3
CAACGCA	6645	0.0	30.509697	5
TATCAAC	6905	0.0	29.62701	2
AACGCAG	7165	0.0	28.611584	6
GTACATG	10200	0.0	23.373	1
ACGCAGA	8475	0.0	23.210478	7
TACATGG	10865	0.0	21.826166	2
CGCAGAG	8885	0.0	21.725668	8
ACATGGG	10915	0.0	21.011492	3
GTGTAGC	2285	0.0	20.141819	1
CATGGGG	7465	0.0	18.740479	4
GCAGAGT	10315	0.0	17.913906	9
GAGTACA	8120	0.0	17.570759	1
AGAGTAC	8410	0.0	17.446747	10-11
GTATATA	1425	0.0	17.440693	1
AGTACAT	7725	0.0	17.041195	2
GTAGCCC	1860	0.0	17.01558	3
>>END_MODULE