FastQCFastQC Report
Mon 15 Aug 2016
SRR2920867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3616703
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32050.044.361461
GTATCAA55950.040.8796961
TCAACGC69100.031.9419294
CAACGCA71350.030.9307965
ATCAACG72100.030.87623
TATCAAC74000.030.210882
AACGCAG76600.028.8728266
GTACATG104300.024.2999611
ACGCAGA91000.023.8342197
TACATGG109300.022.4514622
CGCAGAG94600.022.4224178
ACATGGG111850.021.1338983
GCAGAGT109550.018.3662079
AGAGTAC89250.017.70402310-11
CATGGGG75500.017.6663024
GTATAGC13150.017.3571381
CAGAGTA97400.016.58800310-11
GAGTACA81450.016.171541
ATACCGA2709.5636005E-615.8201616
AGTACAT78700.015.5603442