##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920867_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3616703
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.235387589193806	31.0	30.0	34.0	27.0	34.0
2	30.24325276363583	31.0	30.0	34.0	26.0	34.0
3	30.386568927556397	31.0	30.0	34.0	27.0	34.0
4	34.028366996128796	35.0	35.0	37.0	30.0	37.0
5	33.55453157198697	35.0	35.0	37.0	28.0	37.0
6	33.49371070834404	35.0	35.0	37.0	28.0	37.0
7	33.31175327363071	35.0	33.0	37.0	27.0	37.0
8	33.24363349713814	35.0	33.0	37.0	27.0	37.0
9	34.573884833783694	38.0	34.0	39.0	27.0	39.0
10-11	34.38261076455545	37.5	34.0	39.0	27.0	39.0
12-13	34.20342007624071	37.0	34.0	39.0	26.0	39.0
14-15	35.0962102777032	38.0	34.0	40.0	26.0	41.0
16-17	34.968681835362204	38.0	34.0	40.0	25.5	41.0
18-19	34.882655694979654	38.0	34.0	40.0	25.0	41.0
20-21	34.771368702378936	38.0	34.0	40.0	25.0	41.0
22-23	34.62633398429453	38.0	33.5	40.0	24.5	41.0
24-25	34.46153444172773	38.0	33.0	40.0	24.0	41.0
26-27	34.28334701522354	38.0	33.0	40.0	23.0	41.0
28-29	34.225922753402756	38.0	33.0	40.0	23.5	41.0
30-31	34.216439530699645	38.0	33.0	40.0	23.0	41.0
32-33	34.073551795654765	38.0	33.0	40.0	22.5	41.0
34-35	33.905001046533265	38.0	33.0	40.0	21.5	41.0
36-37	33.71893641805811	38.0	33.0	40.0	19.5	41.0
38-39	33.542466163243155	38.0	32.0	40.0	18.5	41.0
40-41	33.32420383979553	38.0	32.0	40.0	17.0	41.0
42-43	33.360637298666774	38.0	32.0	40.0	17.0	41.0
44-45	33.22765319684807	38.0	32.0	40.0	15.5	41.0
46-47	32.99244367038156	38.0	32.0	40.0	14.5	41.0
48-49	32.738607787258175	37.0	31.0	40.0	12.5	41.0
50-51	32.492816109036326	37.0	31.0	40.0	9.0	41.0
52-53	32.238618847054894	37.0	31.0	40.0	8.5	41.0
54-55	31.966678353185205	36.5	30.5	40.0	7.5	41.0
56-57	31.648858366307657	36.0	30.0	39.0	6.5	41.0
58-59	31.324957426694976	36.0	29.5	39.0	2.0	40.5
60-61	30.996200545082083	35.0	29.0	39.0	2.0	40.0
62-63	30.674142858841325	35.0	29.0	39.0	2.0	40.0
64-65	30.275574881321468	35.0	28.0	38.5	2.0	40.0
66-67	29.839998888490427	34.5	28.0	38.0	2.0	40.0
68-69	29.394829351483935	34.0	28.0	37.5	2.0	40.0
70-71	28.966444853226818	34.0	27.0	37.0	2.0	39.0
72-73	28.51584537077001	34.0	26.0	36.0	2.0	39.0
74-75	28.06499690463939	33.5	26.0	36.0	2.0	38.5
76-77	26.604369089748317	31.5	24.5	34.5	2.0	36.5
78-79	27.439880742211898	33.0	26.0	35.0	2.0	37.0
80-81	27.457629227503613	33.0	26.0	35.0	2.0	37.0
82-83	27.104756597376117	33.0	26.0	35.0	2.0	36.0
84-85	26.775803680866247	33.0	25.0	35.0	2.0	36.0
86-87	26.223892589466153	32.5	24.0	35.0	2.0	35.0
88-89	25.850647952015965	32.0	23.0	35.0	2.0	35.0
90-91	25.48386486255576	32.0	20.5	34.5	2.0	35.0
92-93	25.16357301110984	32.0	19.5	34.0	2.0	35.0
94-95	24.76708289289997	32.0	17.5	34.0	2.0	35.0
96-97	24.22288006507584	32.0	6.0	34.0	2.0	35.0
98-99	23.620662658780663	31.0	2.0	34.0	2.0	35.0
100-101	21.444307287604207	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84569.0
3	38326.0
4	20487.0
5	14326.0
6	12168.0
7	12404.0
8	13641.0
9	16193.0
10	18337.0
11	20547.0
12	22777.0
13	24808.0
14	27132.0
15	28830.0
16	30273.0
17	31464.0
18	30581.0
19	30097.0
20	30120.0
21	31851.0
22	34408.0
23	38438.0
24	45565.0
25	52850.0
26	62445.0
27	74077.0
28	87559.0
29	102782.0
30	120323.0
31	143367.0
32	171708.0
33	209661.0
34	262294.0
35	338903.0
36	452360.0
37	540178.0
38	319216.0
39	21638.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.60497400944331	19.709886383940013	14.031687287826891	24.653452318789796
2	15.839896170628332	20.797892445135805	38.54518880870229	24.817022575533574
3	19.1107290127335	24.66590050349972	29.406922236282067	26.816448247484715
4	13.64930545569584	17.01202151217969	36.50917652420341	32.82949650792106
5	14.35804556621812	37.15193854823524	34.39954984038413	14.090466045162511
6	32.95829974933792	35.852705024756204	17.295794545663902	13.893200680241982
7	28.710499340721167	31.59626329287209	21.16002616071562	18.53321120569112
8	26.187154124483346	35.05327763336019	20.18941312935848	18.57015511279798
9	26.256654063460903	15.95294495523347	19.637022136139542	38.15337884516609
10-11	25.339850317348645	25.75178002992103	27.950999160002212	20.957370492728117
12-13	26.07654773936255	22.8135415965983	27.75845159490143	23.35145906913772
14-15	23.21220957592521	24.514723419615798	25.187239998272652	27.085827006186335
16-17	23.327814509986315	27.586235995547682	25.806968824089445	23.278980670376562
18-19	23.36995404482342	26.77002834236918	27.205090972986547	22.654926639820854
20-21	24.286742634047577	26.03702113270643	26.803070503897597	22.8731657293484
22-23	23.79009098920336	25.97327288140964	26.646992303255097	23.589643826131905
24-25	23.629727216667167	26.062467856296102	26.79508840054589	23.512716526490838
26-27	23.513469469516	26.330858180317087	26.694148999452732	23.461523350714188
28-29	23.552215558609603	26.337162448020386	26.667915085570403	23.442706907799607
30-31	23.92013489602088	26.210457815677874	26.395574782165713	23.473832506135533
32-33	23.673122415840304	26.135619688029742	26.36034425868135	23.8309136374486
34-35	23.913766414061975	26.15948456751501	26.440237362217978	23.48651165620504
36-37	24.030979667190795	25.960025227778043	26.380492804538235	23.628502300492926
38-39	23.790650967259342	26.02952164545897	26.449692792287095	23.730134594994592
40-41	23.953410805911915	25.89384363202009	26.528720894890423	23.624024667177572
42-43	23.87351967746525	25.842959955124538	26.402907668634505	23.8806126987757
44-45	23.730495934110536	25.887792375169482	26.532692710576843	23.84901898014314
46-47	23.76989532524682	25.824999965426493	26.367485966613053	24.037618742713633
48-49	23.637796505771984	25.815021461304326	26.470370680173684	24.076811352750013
50-51	23.6434746815079	25.8917965461252	26.468944868446105	23.995783903920795
52-53	23.703888045525055	25.863328051940154	26.50461638006122	23.928167522473572
54-55	23.522930411601056	25.772231588107758	26.557256101717343	24.14758189857384
56-57	23.510783994655633	25.900265365820207	26.400885161054134	24.18806547847003
58-59	23.333612603779386	25.854595215461295	26.564688712994595	24.247103467764724
60-61	23.42385545444074	25.877188433777725	26.542314386646705	24.156641725134833
62-63	23.235222384571397	25.966458427120397	26.547666319723657	24.25065286858455
64-65	23.10502969971783	25.773642290219257	26.447590778632684	24.67373723143023
66-67	23.085672367904962	25.868200101902005	26.353617659473592	24.692509870719437
68-69	23.244981282291057	25.76471118936255	26.30765897045544	24.68264855789095
70-71	23.02184506294387	25.533861368614353	26.39473494988827	25.049558618553508
72-73	23.03066833338082	25.56663071557969	26.36131202012007	25.041388930919418
74-75	22.962748033045337	25.686721841851618	26.258744442147552	25.091785682955496
76-77	22.985173868624713	25.457724793608786	26.45385722766975	25.103244110096746
78-79	22.876905522131434	25.53787412239479	26.409436427435786	25.175783928037998
80-81	22.883253523416993	25.54413715137779	26.32460678544683	25.248002539758392
82-83	22.853305857683544	25.467640481564963	26.36586692505281	25.313186735698682
84-85	22.712766700260783	25.495833550526747	26.30272839633002	25.48867135288245
86-87	22.694207659362135	25.43698282672876	26.326181268798425	25.542628245110677
88-89	22.607283155242808	25.370286717702573	26.291445575138937	25.730984551915682
90-91	22.500964803523864	25.392963366299306	26.298797433400832	25.807274396776002
92-93	22.531106826117274	25.26678156419171	26.28791458772165	25.914197021969365
94-95	22.349138023399224	25.2219279204824	26.392193235828042	26.036740820290333
96-97	22.413396937087697	25.108030895674837	26.352556768065106	26.126015399172363
98-99	22.23592145629095	25.099907785936182	26.299492477019065	26.364678280753807
100-101	22.345122496649065	24.988638963757364	26.343197998632668	26.323040540960907
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	72.0
1	53.0
2	59.5
3	80.5
4	73.0
5	84.5
6	144.5
7	300.5
8	444.0
9	460.0
10	407.0
11	387.5
12	407.0
13	433.0
14	492.0
15	556.0
16	602.0
17	675.0
18	786.5
19	900.0
20	1048.5
21	1257.5
22	1498.5
23	1832.5
24	2252.5
25	2803.5
26	3652.0
27	4989.0
28	6782.5
29	9437.5
30	13511.0
31	19391.5
32	27152.5
33	35349.0
34	44025.0
35	53826.5
36	65195.0
37	75900.0
38	85408.5
39	95664.0
40	106157.5
41	115012.0
42	122408.5
43	131416.5
44	139610.5
45	145850.0
46	153218.0
47	160150.5
48	165491.5
49	168730.5
50	166904.0
51	162455.5
52	158254.5
53	153872.0
54	147062.0
55	136615.5
56	123873.0
57	109715.5
58	97352.5
59	84992.0
60	71243.5
61	58586.5
62	47225.0
63	36360.5
64	26558.5
65	19312.5
66	14180.0
67	10550.0
68	7614.5
69	5181.0
70	3615.0
71	2693.0
72	1899.5
73	1394.0
74	1026.0
75	707.0
76	468.5
77	265.5
78	155.0
79	73.5
80	33.0
81	22.0
82	15.0
83	10.0
84	5.0
85	3.5
86	3.0
87	1.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18630227585732087
2	0.0
3	0.011474539103708542
4	0.014350086252589721
5	0.005751094297762354
6	0.10703118282037537
7	0.14325201709955174
8	0.1242291667300301
9	0.14344556354226487
10-11	0.11594814393108863
12-13	0.11502188595524708
14-15	0.11687440190693014
16-17	0.06652467730969339
18-19	0.07895312388105963
20-21	0.03692589632048858
22-23	0.08384708393252087
24-25	0.057400345010358886
26-27	0.06583344001428926
28-29	0.04046503127295772
30-31	0.024359202290041508
32-33	0.03223930745764858
34-35	0.019644963935385347
36-37	0.023432944314199976
38-39	0.03186603931813035
40-41	0.024497449749122338
42-43	0.01306438488313804
44-45	0.02628084197126499
46-47	0.033677081032089176
48-49	0.008585167208919284
50-51	0.01552518965477674
52-53	0.006442331593166484
54-55	0.012345498095917748
56-57	0.005142805477806721
58-59	0.003787980378814627
60-61	0.023405294822383814
62-63	0.009815569594738633
64-65	0.007036795667214035
66-67	0.04050650551068197
68-69	0.09069033315702174
70-71	0.021649552092057323
72-73	0.04440508385676126
74-75	0.08632171345006764
76-77	0.24190540389962906
78-79	0.0819116195053893
80-81	0.016893839499676915
82-83	0.011336291644627718
84-85	0.013672673703093673
86-87	0.2586056969565928
88-89	0.6275743404974088
90-91	0.26961019469942654
92-93	0.35701023833032464
94-95	0.0012304023858193498
96-97	0.0040091763133439495
98-99	0.00326264003430749
100-101	0.004852485813736987
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3616703.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.41429309865647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03472414018334	35.30751109886755
2	13.687846352686556	13.801261958274319
3	6.0297687429697335	9.119595861755807
4	3.2175654329596806	6.488451472053394
5	1.9668159526840985	4.957781795486469
6	1.2539799712482387	3.7931108286212107
7	0.8510361277247837	3.0033069346464023
8	0.6169197160984112	2.488125710858019
9	0.42876003564017257	1.9454070695178647
>10	1.835507643566511	15.24065189088514
>50	0.05977836096817879	2.0273437278883084
>100	0.016471524705687527	1.3687774329085265
>500	4.955991444575649E-4	0.18316986618779327
>1k	3.3039942015510173E-4	0.27550435204916246
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9354644271315615E-4
2	0.0	0.0	0.0	0.0	1.9354644271315615E-4
3	0.0	0.0	0.0	2.7649491816165165E-5	1.9354644271315615E-4
4	0.0	0.0	0.0	2.7649491816165165E-5	1.9354644271315615E-4
5	0.0	0.0	0.0	2.7649491816165165E-5	1.9354644271315615E-4
6	0.0	0.0	0.0	2.7649491816165165E-5	3.0414440997781684E-4
7	0.0	0.0	0.0	2.7649491816165165E-5	3.0414440997781684E-4
8	0.0	0.0	0.0	2.7649491816165165E-5	3.0414440997781684E-4
9	0.0	0.0	0.0	2.7649491816165165E-5	3.5944339361014717E-4
10-11	0.0	0.0	0.0	1.1059796726466066E-4	4.4239186905864264E-4
12-13	0.0	0.0	0.0	1.3824745908082582E-4	4.97690852690973E-4
14-15	0.0	0.0	0.0	1.3824745908082582E-4	5.944640740475511E-4
16-17	0.0	0.0	0.0	1.3824745908082582E-4	6.082888199556337E-4
18-19	0.0	0.0	0.0	1.520722049889084E-4	6.359383117717988E-4
20-21	0.0	0.0	0.0	1.7972169680507358E-4	6.497630576798813E-4
22-23	0.0	0.0	0.0	3.456186477020646E-4	6.912372954041291E-4
24-25	1.3824745908082582E-5	0.0	0.0	7.050620413122117E-4	6.912372954041291E-4
26-27	2.7649491816165165E-5	0.0	0.0	0.0015760210335214143	6.912372954041291E-4
28-29	2.7649491816165165E-5	0.0	0.0	0.005364001412336042	6.912372954041291E-4
30-31	4.147423772424775E-5	0.0	0.0	0.022437562608818032	6.912372954041291E-4
32-33	5.529898363233033E-5	0.0	0.0	0.06122979962689776	9.95381705381946E-4
34-35	5.529898363233033E-5	2.7649491816165165E-5	0.0	0.11464861781572885	0.0010092064512900287
36-37	5.529898363233033E-5	2.7649491816165165E-5	0.0	0.20499333232504852	0.0010230311971981111
38-39	5.529898363233033E-5	2.7649491816165165E-5	0.0	0.3551991966163658	0.0010230311971981111
40-41	5.529898363233033E-5	2.7649491816165165E-5	0.0	0.5334139961174583	0.0010506806890142762
42-43	5.529898363233033E-5	2.7649491816165165E-5	0.0	0.7203107360488268	0.0011059796726466066
44-45	5.529898363233033E-5	2.7649491816165165E-5	0.0	0.9514466628860595	0.0011059796726466066
46-47	5.529898363233033E-5	2.7649491816165165E-5	0.0	1.225052209152922	0.0011336291644627717
48-49	5.529898363233033E-5	2.7649491816165165E-5	0.0	1.5176253068056735	0.0011751034021870196
50-51	5.529898363233033E-5	2.7649491816165165E-5	0.0	1.830576079926939	0.0012165776399112674
52-53	5.529898363233033E-5	2.7649491816165165E-5	0.0	2.150176555829992	0.0012165776399112674
54-55	5.529898363233033E-5	2.7649491816165165E-5	0.0	2.5148595281393025	0.0012304023858193498
56-57	5.529898363233033E-5	2.7649491816165165E-5	0.0	2.936113360704487	0.0012442271317274324
58-59	5.529898363233033E-5	2.7649491816165165E-5	0.0	3.3930626872043406	0.0012442271317274324
60-61	5.529898363233033E-5	2.7649491816165165E-5	0.0	3.8750209790519152	0.0012442271317274324
62-63	5.529898363233033E-5	2.7649491816165165E-5	0.0	4.368785603904993	0.0012442271317274324
64-65	5.529898363233033E-5	2.7649491816165165E-5	0.0	4.896918547085564	0.0012442271317274324
66-67	5.529898363233033E-5	2.7649491816165165E-5	0.0	5.501018469031049	0.0012442271317274324
68-69	5.529898363233033E-5	2.7649491816165165E-5	0.0	6.162048694626018	0.001258051877635515
70-71	5.529898363233033E-5	2.7649491816165165E-5	0.0	6.8706360461447895	0.0012857013694516802
72-73	5.529898363233033E-5	2.7649491816165165E-5	0.0	7.585707203494453	0.0012995261153597628
74-75	5.529898363233033E-5	2.7649491816165165E-5	0.0	8.329616780808376	0.0013133508612678453
76-77	5.529898363233033E-5	2.7649491816165165E-5	0.0	9.137203137774929	0.0013824745908082583
78-79	5.529898363233033E-5	2.7649491816165165E-5	0.0	10.018945431792437	0.0014654230662567538
80-81	5.529898363233033E-5	2.7649491816165165E-5	0.0	10.94173339641104	0.0014654230662567538
82-83	5.529898363233033E-5	2.7649491816165165E-5	0.0	11.871295486524605	0.0015068973039810015
84-85	5.529898363233033E-5	2.7649491816165165E-5	0.0	12.812608610660039	0.0015621962876133317
86-87	5.529898363233033E-5	2.7649491816165165E-5	0.0	13.789866074156489	0.0015760210335214143
88-89	5.529898363233033E-5	2.7649491816165165E-5	0.0	14.829459316952484	0.0016036705253375796
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3205	0.0	44.36146	1
GTATCAA	5595	0.0	40.879696	1
TCAACGC	6910	0.0	31.941929	4
CAACGCA	7135	0.0	30.930796	5
ATCAACG	7210	0.0	30.8762	3
TATCAAC	7400	0.0	30.21088	2
AACGCAG	7660	0.0	28.872826	6
GTACATG	10430	0.0	24.299961	1
ACGCAGA	9100	0.0	23.834219	7
TACATGG	10930	0.0	22.451462	2
CGCAGAG	9460	0.0	22.422417	8
ACATGGG	11185	0.0	21.133898	3
GCAGAGT	10955	0.0	18.366207	9
AGAGTAC	8925	0.0	17.704023	10-11
CATGGGG	7550	0.0	17.666302	4
GTATAGC	1315	0.0	17.357138	1
CAGAGTA	9740	0.0	16.588003	10-11
GAGTACA	8145	0.0	16.17154	1
ATACCGA	270	9.5636005E-6	15.820161	6
AGTACAT	7870	0.0	15.560344	2
>>END_MODULE