FastQCFastQC Report
Mon 15 Aug 2016
SRR2920866_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920866_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2633983
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32150.040.3692971
GTATCAA55400.037.965531
TCAACGC69650.029.639454
ATCAACG69500.029.6374173
CAACGCA70000.029.3601845
TATCAAC70850.028.879542
AACGCAG72150.028.7988196
GTACATG91850.025.855541
TACATGG95850.024.075272
ACGCAGA85000.023.8497147
CGCAGAG85400.023.307038
ACATGGG99400.022.476123
GTCTACG2758.0035534E-1121.7567351
TAAGGTG11400.021.3279845
TAAGGCG7750.020.7178695
CATGGGG67250.020.6694584
GCAGAGT94250.019.6100529
GTATAGG6550.018.9716681
GTAAGGT9450.018.9326574
GTATAGA9050.018.3078331
Produced by FastQC (version 0.11.5)