##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920866_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2633983
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.020203623182077	31.0	28.0	31.0	16.0	34.0
2	28.044969538527774	31.0	28.0	33.0	16.0	34.0
3	28.101483570698825	31.0	28.0	33.0	16.0	34.0
4	31.52478167095232	35.0	32.0	37.0	17.0	37.0
5	31.464347340130896	35.0	32.0	37.0	17.0	37.0
6	31.59156684002896	35.0	32.0	37.0	18.0	37.0
7	31.411800683603502	35.0	32.0	37.0	17.0	37.0
8	31.341211769400182	35.0	32.0	37.0	17.0	37.0
9	32.54034896960231	37.0	32.0	39.0	17.0	39.0
10-11	32.33020429516819	37.0	32.0	39.0	16.0	39.0
12-13	32.14559148635355	37.0	32.0	39.0	12.5	39.0
14-15	32.832109015130314	38.0	32.0	40.0	10.5	41.0
16-17	32.712740552995214	38.0	31.5	40.0	10.0	41.0
18-19	32.60376832348577	38.0	31.0	40.0	10.0	41.0
20-21	32.51442701034897	38.0	31.0	40.0	10.0	41.0
22-23	32.28511763363697	37.0	31.0	40.0	9.5	41.0
24-25	32.07902271958475	37.0	30.5	40.0	8.0	41.0
26-27	32.08881169696235	37.0	30.5	40.0	8.0	41.0
28-29	31.91657994755471	37.0	30.0	40.0	4.5	41.0
30-31	31.654636533341332	37.0	30.0	40.0	2.0	41.0
32-33	31.389763525428982	37.0	30.0	40.0	2.0	41.0
34-35	31.05408804840426	36.5	29.5	40.0	2.0	41.0
36-37	30.741694991957047	36.0	29.0	40.0	2.0	41.0
38-39	30.527785107193175	36.0	28.0	39.0	2.0	41.0
40-41	30.313658060815122	36.0	28.0	39.0	2.0	40.0
42-43	30.066983917511998	35.5	27.0	39.0	2.0	40.0
44-45	29.775407434292475	35.0	27.0	39.0	2.0	40.0
46-47	29.505877410750184	35.0	26.0	39.0	2.0	40.0
48-49	29.19334255384336	35.0	26.0	39.0	2.0	40.0
50-51	28.10057449117933	34.0	24.0	38.0	2.0	39.5
52-53	28.558561311899126	34.5	25.0	38.0	2.0	39.5
54-55	29.190272868124055	35.0	26.0	39.0	2.0	40.0
56-57	29.13603143983845	35.0	26.0	39.0	2.0	40.0
58-59	28.89173981001396	35.0	26.0	39.0	2.0	40.0
60-61	28.560635926655564	34.0	25.5	39.0	2.0	40.0
62-63	28.162221434230972	34.0	24.5	38.0	2.0	40.0
64-65	27.743969304281766	34.0	23.0	38.0	2.0	40.0
66-67	27.35240888039141	34.0	22.5	37.0	2.0	40.0
68-69	26.908981189324304	33.0	21.5	37.0	2.0	39.0
70-71	26.57409083505854	33.0	20.5	36.0	2.0	39.0
72-73	26.116429566933427	32.5	19.5	36.0	2.0	39.0
74-75	25.681578051187117	32.0	18.0	35.0	2.0	38.5
76-77	25.260983461168884	32.0	15.0	35.0	2.0	37.0
78-79	24.799415941560746	32.0	9.5	35.0	2.0	37.0
80-81	24.350913236721723	31.0	7.0	35.0	2.0	36.0
82-83	23.872757910738223	31.0	2.0	34.5	2.0	36.0
84-85	23.475618483490592	31.0	2.0	34.0	2.0	35.0
86-87	23.022457244408944	30.5	2.0	34.0	2.0	35.0
88-89	22.489026884380042	30.0	2.0	34.0	2.0	35.0
90-91	21.991268736358585	30.0	2.0	34.0	2.0	35.0
92-93	21.45163313506579	29.0	2.0	34.0	2.0	35.0
94-95	20.82112716748741	29.0	2.0	34.0	2.0	35.0
96-97	20.183155699941874	29.0	2.0	34.0	2.0	35.0
98	19.785721472006465	28.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	164049.0
3	27477.0
4	19637.0
5	15790.0
6	14882.0
7	15193.0
8	17497.0
9	19615.0
10	21054.0
11	22763.0
12	23323.0
13	22775.0
14	21892.0
15	23224.0
16	25109.0
17	26620.0
18	27186.0
19	26855.0
20	27431.0
21	29188.0
22	31590.0
23	34903.0
24	39461.0
25	45600.0
26	51658.0
27	59660.0
28	68248.0
29	78998.0
30	90782.0
31	105624.0
32	124502.0
33	150152.0
34	182795.0
35	227409.0
36	277075.0
37	290835.0
38	166902.0
39	16228.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.11288777023755	18.029337164790373	13.377637412943514	24.480137652028567
2	15.750344330517803	19.351583958356038	39.85294893883163	25.04512277229452
3	19.590113885943943	23.64766137386122	28.712305089173697	28.049919651021142
4	13.211184539278332	15.39898024122444	36.40868420133325	34.98115101816398
5	14.310239615345619	36.97878317585172	34.01473895821759	14.696238250585072
6	33.814947794931584	34.73525318366425	17.080997127249738	14.368801894154437
7	29.478557669561688	31.500315727641148	20.563988671016354	18.457137931780807
8	26.87010826273133	34.370474656557995	19.726947373696643	19.03246970701403
9	27.04094901144009	15.922691983964969	19.332698806332463	37.703660198262476
10-11	25.760364770836556	25.338513069088346	27.324080551028718	21.577041609046375
12-13	26.335510435759023	22.625811430214863	27.349044792751304	23.689633341274803
14-15	23.385269339608406	24.206461523333424	24.91956363807557	27.488705498982597
16-17	23.488088794949284	27.235046425499466	25.718935084791482	23.557929694759768
18-19	23.43859367944195	26.417313609013505	27.1975752622362	22.94651744930834
20-21	24.173460854724624	25.71191274868172	27.078102102978345	23.036524293615308
22-23	23.79946354228913	25.460650806007095	26.963431611712085	23.776454039991695
24-25	23.57287568204551	25.76621020316912	26.884056346845902	23.776857767939465
26-27	23.374388489536624	25.86778779530259	26.911380396598243	23.846443318562542
28-29	23.454005634885693	26.194659204134123	26.680579777088877	23.670755383891308
30-31	23.78903854470204	25.946106814354476	26.491161731025585	23.773692909917894
32-33	23.38606065841598	25.88640477991887	26.58097012835495	24.146564433310203
34-35	23.908935316471027	26.007640518589444	26.46705421485312	23.616369950086412
36-37	24.032574204471153	25.66412648807594	26.51397873910194	23.789320568350973
38-39	23.834971382987504	25.836646515881828	26.446927972908075	23.881454128222597
40-41	23.957763486737544	25.72289996295143	26.573796727103062	23.745539823207963
42-43	23.821917534084445	25.628185812752115	26.488980625684334	24.06091602747911
44-45	23.69079791915608	25.708609062048225	26.59364035835698	24.006952660438717
46-47	23.88084841731559	25.765466528154946	26.349422236923637	24.004262817605827
48-49	23.761030678895573	25.721703666290253	26.332774269684606	24.184491385129565
50-51	23.690027943758874	25.95953670554762	26.428638247112602	23.921797103580904
52-53	23.840303783859003	25.690247596426495	26.47978825106013	23.98966036865437
54-55	23.389541270867333	25.691755662938426	26.722519400688434	24.196183665505806
56-57	23.49748926441136	25.81812589296485	26.542445810361855	24.141939032261938
58-59	23.445284237664552	25.522367156156122	26.62027425764365	24.412074348535675
60-61	23.33825524280552	25.50785301201421	26.88509831558638	24.268793429593888
62-63	23.233378904296213	25.494314744508856	26.998023141157248	24.274283210037677
64-65	23.139207467170333	25.511605594506374	26.756508887414267	24.592678050909026
66-67	23.140841737867756	25.603869063337303	26.70118536395541	24.55410383483953
68-69	23.404326272786832	25.18601631432536	26.816515100641087	24.593142312246723
70-71	23.097157107687945	25.158732154299923	26.830362891458677	24.91374784655346
72-73	23.054923166381958	25.176754176038223	26.812657619813912	24.955665037765904
74-75	22.998347063371458	25.288349843656572	26.927651649318452	24.78565144365352
76-77	23.09905801490929	25.036728601769028	26.911901057193404	24.952312326128276
78-79	23.034466683970116	25.040841793203704	26.96032514237738	24.9643663804488
80-81	23.078471637361076	25.076382706977995	26.928994269916164	24.916151385744765
82-83	23.00310945996106	24.98135980929204	27.053939522175018	24.96159120857188
84-85	22.96815797317153	24.948440846238597	26.996294825611127	25.08710635497875
86-87	22.95620149540078	24.889235037661134	27.13147930041979	25.023084166518295
88-89	22.952939842970885	24.669592111015756	27.053165680670638	25.32430236534272
90-91	22.81585027598813	24.699818509307946	27.20453861957749	25.279792595126434
92-93	22.98829801051324	24.378859098874823	27.24983250496772	25.383010385644216
94-95	22.749079096651	24.31906670366596	27.415675040581455	25.516179159101586
96-97	22.90168758619389	24.049345997736985	27.42236621650782	25.6266001995613
98	22.853367654413436	23.93464927098811	27.495588296349172	25.71639477824928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	67.0
1	52.0
2	70.0
3	117.5
4	123.0
5	133.0
6	209.0
7	408.5
8	637.5
9	719.5
10	746.5
11	818.5
12	924.5
13	1058.5
14	1215.0
15	1307.5
16	1414.0
17	1503.0
18	1565.0
19	1605.5
20	1594.0
21	1726.5
22	1957.0
23	2332.0
24	2900.5
25	3633.5
26	4689.0
27	6213.5
28	8267.5
29	11479.0
30	16328.5
31	22644.5
32	28348.5
33	33331.0
34	39475.5
35	46548.0
36	54347.5
37	61460.0
38	67071.0
39	73261.5
40	78905.0
41	83417.0
42	89595.5
43	96031.5
44	101813.5
45	105609.5
46	108281.0
47	112727.0
48	115015.5
49	114216.5
50	113654.0
51	112574.0
52	110128.5
53	108972.5
54	106512.5
55	101752.0
56	94987.5
57	86581.5
58	78626.5
59	68378.0
60	57277.0
61	47166.5
62	38518.5
63	30201.0
64	22295.5
65	16910.0
66	12657.0
67	9137.0
68	6579.0
69	4746.5
70	3436.5
71	2456.5
72	1859.0
73	1435.5
74	1046.0
75	716.0
76	446.5
77	268.0
78	156.5
79	82.5
80	42.0
81	32.5
82	25.0
83	20.0
84	13.5
85	9.0
86	10.5
87	8.5
88	5.5
89	7.0
90	8.0
91	5.0
92	2.5
93	1.5
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3566461894400989
2	0.023234774104464607
3	0.01788166438431835
4	0.015907467891782143
5	0.02103278570894345
6	0.023462566007449556
7	0.014578681791036616
8	0.007782890018652361
9	0.0
10-11	1.8982658582078926E-5
12-13	0.0049544738899226
14-15	0.002600624225744813
16-17	0.0052961617444000205
18-19	0.03629484320893491
20-21	0.07418422973876446
22-23	0.09345162819957456
24-25	0.06241498141787552
26-27	0.07112802170704974
28-29	0.055201571156685524
30-31	0.04982947877795719
32-33	0.017672855139915483
34-35	0.3130240400184815
36-37	0.4800714355407761
38-39	0.43677199131505406
40-41	0.44607349402027274
42-43	0.39998359898298513
44-45	0.3778118537591169
46-47	0.3757427439736703
48-49	0.366536154561362
50-51	0.3646378887031541
52-53	0.42010521707998877
54-55	0.420408939617302
56-57	0.39292204999045166
58-59	0.3792925011285191
60-61	0.3925613794773923
62-63	0.3747366630688201
64-65	0.42097841937476443
66-67	0.43810077741579956
68-69	0.38549983048485886
70-71	0.3462816578542838
72-73	0.3339998777516787
74-75	0.31712429427221056
76-77	0.3152829763897489
78-79	0.3152070457554206
80-81	0.33090570440279987
82-83	0.32678646749048873
84-85	0.32652071027033963
86-87	0.3138023290203467
88-89	0.3534760854568917
90-91	0.3333165020427239
92-93	0.3239200860445948
94-95	0.33058299920690454
96-97	0.3312284095986952
98	0.3257424212684744
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2633983.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.25716390079997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51981609624842	36.948974475058286
2	11.205829322678087	11.263464018284457
3	5.198691261883429	7.838144364543961
4	2.9546573847643884	5.9397080182725155
5	1.8626899288906988	4.680675652631465
6	1.2051865111039766	3.63415536117515
7	0.8596049786832274	3.0240915812538636
8	0.6113986506201385	2.4581729754355393
9	0.4552460697145886	2.059143870675698
>10	2.030822209752477	16.710323797234928
>50	0.07030062395424465	2.4264771588240968
>100	0.023623150110449038	2.0533149432078805
>500	0.0013717360259412525	0.46729533301611575
>1k	7.620755699570542E-4	0.4960584503860958
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.59306343283157E-5
2	0.0	0.0	0.0	0.0	7.59306343283157E-5
3	0.0	0.0	0.0	0.0	7.59306343283157E-5
4	0.0	0.0	0.0	0.0	7.59306343283157E-5
5	0.0	0.0	0.0	0.0	7.59306343283157E-5
6	0.0	0.0	0.0	0.0	7.59306343283157E-5
7	0.0	0.0	0.0	0.0	7.59306343283157E-5
8	0.0	0.0	0.0	0.0	7.59306343283157E-5
9	0.0	0.0	0.0	0.0	7.59306343283157E-5
10-11	0.0	0.0	0.0	0.0	7.59306343283157E-5
12-13	0.0	0.0	0.0	1.8982658582078926E-5	1.518612686566314E-4
14-15	0.0	0.0	0.0	7.59306343283157E-5	1.8982658582078927E-4
16-17	0.0	0.0	0.0	7.59306343283157E-5	2.2779190298494713E-4
18-19	0.0	0.0	0.0	7.59306343283157E-5	2.4677456156702607E-4
20-21	0.0	0.0	0.0	1.8982658582078927E-4	2.65757220149105E-4
22-23	0.0	0.0	0.0	3.7965317164157853E-4	2.65757220149105E-4
24-25	0.0	0.0	0.0	8.921849533577097E-4	2.65757220149105E-4
26-27	0.0	0.0	0.0	0.0019172485167899716	2.65757220149105E-4
28-29	0.0	0.0	0.0	0.0067198611380559405	2.65757220149105E-4
30-31	0.0	0.0	0.0	0.025038126669762108	2.65757220149105E-4
32-33	0.0	0.0	0.0	0.06541424147384399	2.65757220149105E-4
34-35	0.0	0.0	0.0	0.11955278374993308	2.65757220149105E-4
36-37	0.0	0.0	0.0	0.21106818077413558	2.65757220149105E-4
38-39	0.0	0.0	0.0	0.3651124551677061	2.65757220149105E-4
40-41	0.0	0.0	0.0	0.5393352956340265	2.65757220149105E-4
42-43	0.0	0.0	0.0	0.715987916398853	2.65757220149105E-4
44-45	0.0	0.0	0.0	0.9293150335442559	2.847398787311839E-4
46-47	0.0	0.0	0.0	1.1885422191411257	3.416878544774207E-4
48-49	0.0	0.0	0.0	1.4747057972659658	3.606705130594996E-4
50-51	0.0	0.0	0.0	1.7903494441687742	3.7965317164157853E-4
52-53	0.0	0.0	0.0	2.1015891142805403	4.176184888057364E-4
54-55	0.0	0.0	0.0	2.446674864644153	4.5558380596989425E-4
56-57	0.0	0.0	0.0	2.8525241051290005	4.5558380596989425E-4
58-59	0.0	0.0	0.0	3.2916309634496503	4.5558380596989425E-4
60-61	0.0	0.0	0.0	3.767829936639682	4.745664645519732E-4
62-63	0.0	0.0	0.0	4.248015268131951	5.504970988802889E-4
64-65	0.0	0.0	0.0	4.747619859353685	6.074450746265256E-4
66-67	0.0	0.0	0.0	5.330748148336568	6.074450746265256E-4
68-69	0.0	0.0	0.0	5.969571557599271	6.074450746265256E-4
70-71	0.0	0.0	0.0	6.653725555555978	6.074450746265256E-4
72-73	0.0	0.0	0.0	7.349193218027603	6.074450746265256E-4
74-75	0.0	0.0	0.0	8.043180233129826	6.454103917906836E-4
76-77	0.0	0.0	0.0	8.799202576478283	6.454103917906836E-4
78-79	0.0	0.0	0.0	9.633946004966623	6.454103917906836E-4
80-81	0.0	0.0	0.0	10.517474866010904	6.643930503727625E-4
82-83	0.0	0.0	0.0	11.414519379965625	6.833757089548414E-4
84-85	0.0	0.0	0.0	12.306286714834531	6.833757089548414E-4
86	0.0	0.0	0.0	12.98060769564572	7.213410261189992E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3215	0.0	40.369297	1
GTATCAA	5540	0.0	37.96553	1
TCAACGC	6965	0.0	29.63945	4
ATCAACG	6950	0.0	29.637417	3
CAACGCA	7000	0.0	29.360184	5
TATCAAC	7085	0.0	28.87954	2
AACGCAG	7215	0.0	28.798819	6
GTACATG	9185	0.0	25.85554	1
TACATGG	9585	0.0	24.07527	2
ACGCAGA	8500	0.0	23.849714	7
CGCAGAG	8540	0.0	23.30703	8
ACATGGG	9940	0.0	22.47612	3
GTCTACG	275	8.0035534E-11	21.756735	1
TAAGGTG	1140	0.0	21.327984	5
TAAGGCG	775	0.0	20.717869	5
CATGGGG	6725	0.0	20.669458	4
GCAGAGT	9425	0.0	19.610052	9
GTATAGG	655	0.0	18.971668	1
GTAAGGT	945	0.0	18.932657	4
GTATAGA	905	0.0	18.307833	1
>>END_MODULE