##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920866_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2633983
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.26783012646627	31.0	30.0	34.0	27.0	34.0
2	30.26986165058772	31.0	30.0	34.0	26.0	34.0
3	30.40387010850108	31.0	30.0	34.0	27.0	34.0
4	34.06148748871956	35.0	35.0	37.0	30.0	37.0
5	33.598973493754514	35.0	35.0	37.0	28.0	37.0
6	33.55881188299241	35.0	35.0	37.0	28.0	37.0
7	33.372104907283	35.0	33.0	37.0	27.0	37.0
8	33.302878188659534	35.0	33.0	37.0	27.0	37.0
9	34.62611603795469	38.0	34.0	39.0	27.0	39.0
10-11	34.43276684018082	37.0	34.0	39.0	27.0	39.0
12-13	34.26435724907867	37.0	34.0	39.0	27.0	39.0
14-15	35.15125837942007	38.0	34.0	40.0	26.5	41.0
16-17	35.0315854354413	38.0	34.0	40.0	26.0	41.0
18-19	34.94814488172475	38.0	34.0	40.0	25.0	41.0
20-21	34.835470274485445	38.0	34.0	40.0	25.0	41.0
22-23	34.700017236254	38.0	34.0	40.0	25.0	41.0
24-25	34.523079306130676	38.0	33.0	40.0	24.0	41.0
26-27	34.33981312711585	38.0	33.0	40.0	24.0	41.0
28-29	34.27885202751878	38.0	33.0	40.0	23.5	41.0
30-31	34.26020688060629	38.0	33.0	40.0	23.5	41.0
32-33	34.110025197581	38.0	33.0	40.0	23.0	41.0
34-35	33.94465245219882	38.0	33.0	40.0	22.0	41.0
36-37	33.75029280750863	38.0	33.0	40.0	20.5	41.0
38-39	33.56275932684456	38.0	32.0	40.0	19.0	41.0
40-41	33.32282763404319	38.0	32.0	40.0	18.0	41.0
42-43	33.33846991419459	38.0	32.0	40.0	18.0	41.0
44-45	33.18614603814831	38.0	32.0	40.0	15.5	41.0
46-47	32.93368028571179	37.5	31.5	40.0	15.0	41.0
48-49	32.66451283094841	37.0	31.0	40.0	12.5	41.0
50-51	32.40475432073784	37.0	31.0	40.0	9.0	41.0
52-53	32.14206488804218	37.0	31.0	40.0	8.0	41.0
54-55	31.85607329280409	36.0	30.0	39.5	7.5	41.0
56-57	31.535650381950074	36.0	30.0	39.0	6.5	41.0
58-59	31.199185605981512	35.5	29.5	39.0	2.0	40.0
60-61	30.86484707760073	35.0	29.0	39.0	2.0	40.0
62-63	30.53762324965651	35.0	28.5	39.0	2.0	40.0
64-65	30.13144978536308	35.0	28.0	38.0	2.0	40.0
66-67	29.698071703575913	34.0	28.0	38.0	2.0	40.0
68-69	29.247611127330735	34.0	28.0	37.0	2.0	39.5
70-71	28.815560313031632	34.0	26.5	36.5	2.0	39.0
72-73	28.36760810529149	34.0	26.0	36.0	2.0	39.0
74-75	27.92178480271133	33.0	26.0	35.5	2.0	38.5
76-77	26.489398375008495	31.5	24.5	34.0	2.0	36.0
78-79	27.322377934861386	33.0	26.0	35.0	2.0	37.0
80-81	27.33979072757873	33.0	26.0	35.0	2.0	37.0
82-83	26.987634316546462	33.0	25.0	35.0	2.0	36.0
84-85	26.656325610301963	33.0	25.0	35.0	2.0	36.0
86-87	26.10842420015619	32.0	24.0	35.0	2.0	35.0
88-89	25.727420222529908	32.0	22.0	35.0	2.0	35.0
90-91	25.348131897586278	32.0	20.0	34.5	2.0	35.0
92-93	25.021686738297095	32.0	19.0	34.0	2.0	35.0
94-95	24.623518261127728	32.0	16.5	34.0	2.0	35.0
96-97	24.080263046496505	31.0	4.5	34.0	2.0	35.0
98-99	23.45626395462689	31.0	2.0	34.0	2.0	35.0
100-101	21.24976622855956	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57114.0
3	26123.0
4	14255.0
5	10237.0
6	8848.0
7	8963.0
8	10194.0
9	11868.0
10	13637.0
11	15049.0
12	16979.0
13	18508.0
14	20175.0
15	21832.0
16	23699.0
17	24316.0
18	23273.0
19	22657.0
20	22638.0
21	23721.0
22	25737.0
23	28856.0
24	33731.0
25	39427.0
26	46499.0
27	55768.0
28	65561.0
29	76822.0
30	90654.0
31	107425.0
32	127786.0
33	156263.0
34	194345.0
35	251625.0
36	329404.0
37	377598.0
38	216153.0
39	16243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.31757684743248	19.321216353396828	14.059507767182676	24.30169903198802
2	15.95302627237913	20.409926715548277	38.61308140561272	25.023965606459875
3	19.45586295458981	24.426933390238144	29.11394808246236	27.00325557270969
4	13.80612075580881	16.41184084774279	35.89491914009149	33.88711925635691
5	14.277399322501353	37.306923896444914	34.02407010994735	14.391606671106377
6	33.813761723126696	35.279219071393065	16.78605797370249	14.120961231777752
7	29.437159487953522	31.670462278964195	20.51459378077202	18.37778445231025
8	26.890108169092898	34.923913671515066	19.49366251364658	18.69231564574546
9	26.760964456216886	15.928415902828972	19.290645106223785	38.01997453473036
10-11	25.827616974331736	25.59580155246777	27.23346595222869	21.343115520971807
12-13	26.41682914049807	22.716378749544123	27.350204005303812	23.51658810465399
14-15	23.399447533236852	24.369903769678185	24.82962757430602	27.401021122778946
16-17	23.512943909566918	27.430963757813025	25.681757526196968	23.37433480642309
18-19	23.458278746576614	26.501009158416593	27.190683066504146	22.850029028502647
20-21	24.209811096028908	25.70403170858245	27.040218338322614	23.045938857066027
22-23	23.86281488996484	25.498575579766108	26.970435324481883	23.668174205787164
24-25	23.620651274597922	25.879090029850655	26.841087995234837	23.65917070031659
26-27	23.410751316423568	25.986479506987713	26.86957220436767	23.733196972221045
28-29	23.512372854125545	26.16953694802805	26.701402749286814	23.616687448559592
30-31	23.776729536461765	26.026777432646764	26.50072655996783	23.695766470923648
32-33	23.49511786676004	25.90218491626763	26.608456920806734	23.994240296165597
34-35	23.8229527768506	26.054108838159163	26.524236614335734	23.598701770654504
36-37	24.04457422591406	25.760699771827568	26.53341386552428	23.661312136734093
38-39	23.832295327206445	25.8474539258583	26.54592906517164	23.774321681763613
40-41	23.93323659679281	25.791250343204585	26.617447742710677	23.65806531729193
42-43	23.836040824432082	25.741726498783613	26.498384922040735	23.92384775474357
44-45	23.65822967718219	25.786632605029762	26.649268750150718	23.905868967637332
46-47	23.843309260058213	25.830531789483917	26.396486689311953	23.929672261145914
48-49	23.72742769239298	25.790850658410193	26.38804539796308	24.093676251233752
50-51	23.657649249126553	25.97176492567209	26.433008433917475	23.937577391283877
52-53	23.850482193029084	25.72359328726555	26.468087933783885	23.957836585921484
54-55	23.40530193074148	25.7031229383221	26.621742537046366	24.269832593890058
56-57	23.46910978086654	25.87740804673308	26.41069551567353	24.24278665672685
58-59	23.389512873987663	25.619457552497703	26.504312843766055	24.48671672974858
60-61	23.33602191574408	25.684126503118833	26.733490091358636	24.24636148977845
62-63	23.18894947783249	25.726837923510608	26.73288059641138	24.351332002245524
64-65	23.029551834075676	25.70817445199464	26.525308311745217	24.736965402184467
66-67	23.099293766078933	25.838070013420516	26.352292017632966	24.71034420286759
68-69	23.427537835858256	25.462214491387837	26.401377870286012	24.70886980246789
70-71	23.112302982386986	25.401857830617068	26.35274098748611	25.133098199509835
72-73	23.043109579558394	25.436351671767447	26.325283185849113	25.195255562825047
74-75	23.02949228223629	25.5694775426499	26.249497233430176	25.151532941683634
76-77	23.120756429392976	25.27651170290796	26.442074339603614	25.16065752809545
78-79	23.101552831964426	25.34975669677545	26.337255846629485	25.211434624630634
80-81	23.090121209888927	25.373709289922918	26.261566695056942	25.27460280513121
82-83	23.078157096371225	25.308770650840838	26.303010627755185	25.31006162503275
84-85	22.957450791359314	25.270757163203832	26.194595070763466	25.577196974673388
86-87	22.993203855411416	25.20912531609532	26.259642715230274	25.53802811326299
88-89	22.932494399809304	25.08335345225398	26.133083604047417	25.851068543889298
90-91	22.770380820630812	25.114202792182134	26.17025504528902	25.945161341898036
92-93	22.898418096358927	24.863425036070115	26.17414084165762	26.064016025913332
94-95	22.598596252243304	24.84087581042761	26.30369836318015	26.25682957414893
96-97	22.744697895863865	24.53858595369534	26.333783876136163	26.382932274304633
98-99	22.593546469169286	24.501521797689843	26.285289276342628	26.619642456798235
100-101	22.7067738256328	24.368755324916737	26.356553387368425	26.567917462082036
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	74.0
1	55.0
2	42.0
3	63.5
4	71.5
5	65.0
6	120.0
7	263.5
8	406.5
9	458.0
10	409.0
11	356.0
12	360.0
13	413.0
14	480.0
15	486.0
16	514.0
17	646.0
18	770.0
19	834.0
20	943.5
21	1159.0
22	1392.0
23	1664.0
24	2170.0
25	2788.5
26	3558.5
27	4753.0
28	6327.0
29	8663.5
30	12288.0
31	17365.5
32	23825.0
33	29251.0
34	34084.5
35	40272.5
36	48089.0
37	55201.0
38	61137.0
39	66553.5
40	71879.5
41	77409.5
42	82704.5
43	88787.5
44	94638.5
45	100343.5
46	104273.0
47	106528.5
48	110338.5
49	112992.0
50	112696.0
51	111518.0
52	109885.0
53	109949.0
54	109296.0
55	104166.5
56	96364.5
57	88264.0
58	80589.0
59	70915.5
60	59287.5
61	48842.5
62	39720.5
63	30465.5
64	22576.0
65	17133.0
66	12677.5
67	9055.5
68	6355.5
69	4512.0
70	3342.5
71	2454.0
72	1749.5
73	1345.5
74	1007.5
75	692.0
76	425.0
77	221.0
78	120.5
79	67.0
80	29.5
81	12.0
82	8.5
83	3.5
84	1.5
85	1.5
86	0.5
87	0.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18147421604467456
2	0.0
3	0.013098034421634461
4	0.013439722276111881
5	0.0067198611380559405
6	0.10854284177232731
7	0.14248383531708442
8	0.12479199751858688
9	0.14176249429096544
10-11	0.11429458732269723
12-13	0.11566133874060691
14-15	0.11600302659508432
16-17	0.0668379408674999
18-19	0.07923361692159744
20-21	0.038155143749978644
22-23	0.08392233359137094
24-25	0.05865641501862389
26-27	0.06668607959884328
28-29	0.0410784731716188
30-31	0.025949294281701896
32-33	0.03270712073692199
34-35	0.02088092444028682
36-37	0.02534184920707537
38-39	0.033789132276100496
40-41	0.02710723645520871
42-43	0.01381937544775346
44-45	0.027240115065283264
46-47	0.034586403936547806
48-49	0.009586242583949859
50-51	0.017084392723871036
52-53	0.006947653041040887
54-55	0.013572600886186434
56-57	0.004992439207086758
58-59	0.004024323619400733
60-61	0.023728323227598658
62-63	0.011294681856336962
64-65	0.007744924701488203
66-67	0.04075576797572346
68-69	0.09056626409509856
70-71	0.022361571809688976
72-73	0.04614684301303387
74-75	0.0871114202331602
76-77	0.2319870705315866
78-79	0.07904379033577666
80-81	0.01716032335819935
82-83	0.011883144272381409
84-85	0.01328786100745525
86-87	0.2509887117722476
88-89	0.6156076178168196
90-91	0.2686805495707451
92-93	0.3505717386938336
94-95	0.0012908207835813671
96-97	0.004669734011191416
98-99	0.0034738265205204433
100-101	0.005277179085817942
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2633983.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.01762492991537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.9464519949199	31.038055161557192
2	12.81938919929747	11.541969096091634
3	5.96632905861819	8.057698913079864
4	3.4673397595527575	6.243656032005159
5	2.211802171963108	4.978504029830369
6	1.4666822190971252	3.961593001841421
7	1.0679872690001564	3.3654775214041566
8	0.7582815178327724	2.730882636886615
9	0.563040759127408	2.2812081943187232
>10	2.6047573486271047	19.39169221666543
>50	0.09290301888838162	2.8482843327812866
>100	0.03198276650282473	2.387168979832899
>500	0.0021200809533965157	0.6366297076390465
>1k	9.32835619479847E-4	0.5371801760662689
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	3.796531716415785E-5	0.0
16-17	0.0	0.0	0.0	3.796531716415785E-5	0.0
18-19	0.0	0.0	0.0	3.796531716415785E-5	0.0
20-21	0.0	0.0	0.0	1.1389595149247355E-4	0.0
22-23	0.0	0.0	0.0	4.1761848880573636E-4	0.0
24-25	0.0	0.0	0.0	0.0010060809048501832	0.0
26-27	0.0	0.0	0.0	0.002069109785446603	0.0
28-29	0.0	0.0	0.0	0.006928670382458809	3.796531716415785E-5
30-31	0.0	0.0	0.0	0.025607606427224473	3.796531716415785E-5
32-33	0.0	0.0	0.0	0.06649625301302249	3.796531716415785E-5
34-35	0.0	0.0	0.0	0.12139410163239474	3.796531716415785E-5
36-37	0.0	0.0	0.0	0.21452302463607398	3.796531716415785E-5
38-39	0.0	0.0	0.0	0.37249670935613477	3.796531716415785E-5
40-41	0.0	0.0	0.0	0.5511045439549154	3.796531716415785E-5
42-43	0.0	0.0	0.0	0.7321611415107843	3.796531716415785E-5
44-45	0.0	0.0	0.0	0.9495125822755879	5.6947975746236774E-5
46-47	0.0	0.0	0.0	1.2122325770515605	7.59306343283157E-5
48-49	0.0	0.0	0.0	1.5027431839916963	1.1389595149247355E-4
50-51	0.0	0.0	0.0	1.818045143040027	1.518612686566314E-4
52-53	0.0	0.0	0.0	2.1293607437861217	1.518612686566314E-4
54-55	0.0	0.0	0.0	2.4742946328810778	1.518612686566314E-4
56-57	0.0	0.0	0.0	2.8780557809218967	1.518612686566314E-4
58-59	0.0	0.0	0.0	3.317314500511203	1.518612686566314E-4
60-61	0.0	0.0	0.0	3.7952978436079503	1.518612686566314E-4
62-63	0.0	0.0	0.0	4.274970643318502	1.518612686566314E-4
64-65	0.0	0.0	0.0	4.775657246079417	1.518612686566314E-4
66-67	0.0	0.0	0.0	5.358823500379463	1.518612686566314E-4
68-69	1.8982658582078926E-5	0.0	0.0	5.997381152422016	1.518612686566314E-4
70-71	3.796531716415785E-5	0.0	0.0	6.681554133037306	1.518612686566314E-4
72-73	3.796531716415785E-5	0.0	0.0	7.379983090247736	1.518612686566314E-4
74-75	3.796531716415785E-5	0.0	0.0	8.082056717905925	1.518612686566314E-4
76-77	3.796531716415785E-5	0.0	0.0	8.843280309705872	1.8982658582078927E-4
78-79	3.796531716415785E-5	0.0	0.0	9.682902281449804	1.8982658582078927E-4
80-81	3.796531716415785E-5	0.0	0.0	10.571879165507141	1.8982658582078927E-4
82-83	3.796531716415785E-5	0.0	0.0	11.47748485848238	1.8982658582078927E-4
84-85	3.796531716415785E-5	0.0	0.0	12.381609904088219	1.8982658582078927E-4
86-87	3.796531716415785E-5	0.0	0.0	13.30568572386382	2.0880924440286818E-4
88-89	3.796531716415785E-5	0.0	0.0	14.29117044415245	2.2779190298494713E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3580	0.0	40.77782	1
GTATCAA	5910	0.0	35.080654	1
TCAACGC	7120	0.0	28.93795	4
ATCAACG	7190	0.0	28.788273	3
CAACGCA	7190	0.0	28.586927	5
TATCAAC	7380	0.0	28.367676	2
AACGCAG	7440	0.0	27.690145	6
GTACATG	9845	0.0	24.295225	1
TACATGG	9980	0.0	23.30811	2
ACGCAGA	8855	0.0	23.104532	7
CGCAGAG	9160	0.0	22.021362	8
TAAGGTG	1310	0.0	21.741446	5
ACATGGG	10450	0.0	21.62463	3
CATGGGG	6450	0.0	19.578524	4
GTAAGGT	1095	0.0	19.076406	4
GCAGAGT	10145	0.0	18.900793	9
TAAGGCG	920	0.0	18.574757	5
GTATATA	835	0.0	17.084545	1
AGAGTAC	8765	0.0	17.028082	10-11
ACCGTGC	540	0.0	16.699772	8
>>END_MODULE