FastQCFastQC Report
Mon 15 Aug 2016
SRR2920865_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920865_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3870154
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71900.034.2461781
GGTATCA45000.032.3192861
TCAACGC87750.028.2234364
TATCAAC89150.027.7291322
ATCAACG89500.027.4666063
CAACGCA90550.027.3000535
AACGCAG94700.026.2926586
GTACATG131850.025.7261351
TACATGG134500.024.3810622
ACATGGG139150.023.2693753
ACGCAGA113350.021.1938697
TAAGGCG10700.020.5740935
GTATAGG8550.020.4552441
CATGGGG97500.020.2267954
CGCAGAG120300.019.7402828
GAGTACA99150.018.5211261
CGCCGTA6250.017.6682992
GCAGAGT130150.017.154349
AGAGTAC101050.016.80897110-11
AGTACAT100200.016.7983722
Produced by FastQC (version 0.11.5)