##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920865_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3870154
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.40167703920826	31.0	28.0	33.0	18.0	34.0
2	28.433268805324026	31.0	28.0	33.0	16.0	34.0
3	28.503060084947524	31.0	28.0	34.0	16.0	34.0
4	31.958062392349245	35.0	32.0	37.0	20.0	37.0
5	31.91594184624178	35.0	32.0	37.0	20.0	37.0
6	32.038541877144944	35.0	32.0	37.0	22.0	37.0
7	31.86860057765143	35.0	32.0	37.0	19.0	37.0
8	31.797817089449154	35.0	32.0	37.0	19.0	37.0
9	33.03153259534375	37.0	32.0	39.0	18.0	39.0
10-11	32.832797867991815	37.0	32.0	39.0	17.5	39.0
12-13	32.660672676074384	37.0	32.0	39.0	17.0	39.0
14-15	33.406753193800554	38.0	32.0	40.0	17.0	41.0
16-17	33.30069604465352	38.0	32.0	40.0	17.0	41.0
18-19	33.1771849388939	38.0	32.0	40.0	16.0	41.0
20-21	33.07507634063141	38.0	32.0	40.0	12.0	41.0
22-23	32.852657413632635	38.0	32.0	40.0	10.5	41.0
24-25	32.655787211568324	38.0	31.0	40.0	10.0	41.0
26-27	32.69045004410677	38.0	31.0	40.0	10.0	41.0
28-29	32.525514876152215	38.0	31.0	40.0	9.0	41.0
30-31	32.27401584019654	37.0	30.5	40.0	8.0	41.0
32-33	32.030485996164494	37.0	30.0	40.0	8.0	41.0
34-35	31.70800593464756	37.0	30.0	40.0	4.0	41.0
36-37	31.408250550236502	37.0	30.0	40.0	2.0	41.0
38-39	31.20514442060962	37.0	30.0	40.0	2.0	41.0
40-41	31.00826969676142	36.0	29.5	40.0	2.0	41.0
42-43	30.768652358536634	36.0	29.0	39.5	2.0	41.0
44-45	30.48721885485694	36.0	28.0	39.0	2.0	40.0
46-47	30.237414325114713	35.5	28.0	39.0	2.0	40.0
48-49	29.93656660691022	35.0	27.0	39.0	2.0	40.0
50-51	28.804413080203012	34.0	25.5	38.0	2.0	39.5
52-53	29.261341150765574	34.5	26.5	38.5	2.0	39.5
54-55	29.907725506530234	35.0	28.0	39.0	2.0	40.0
56-57	29.85776793378248	35.0	28.0	39.0	2.0	40.0
58-59	29.612071509299113	35.0	27.5	39.0	2.0	40.0
60-61	29.28718133697005	35.0	27.0	39.0	2.0	40.0
62-63	28.895549892846642	34.0	26.0	38.0	2.0	40.0
64-65	28.466439319985717	34.0	26.0	38.0	2.0	40.0
66-67	28.078559793744642	34.0	25.5	37.5	2.0	40.0
68-69	27.636790680680924	34.0	24.5	37.0	2.0	39.5
70-71	27.30527854963911	33.0	24.0	36.0	2.0	39.0
72-73	26.847560200446804	33.0	22.5	36.0	2.0	39.0
74-75	26.412635388669287	33.0	22.0	35.0	2.0	38.5
76-77	25.998791262569913	32.0	20.5	35.0	2.0	37.0
78-79	25.556306932488994	32.0	19.5	35.0	2.0	37.0
80-81	25.118650058886544	32.0	17.5	35.0	2.0	36.0
82-83	24.65449850316034	31.5	12.5	35.0	2.0	36.0
84-85	24.267565709271516	31.0	7.0	34.0	2.0	35.0
86-87	23.82990444307901	31.0	3.5	34.0	2.0	35.0
88-89	23.316002024725634	31.0	2.0	34.0	2.0	35.0
90-91	22.839595142725585	30.5	2.0	34.0	2.0	35.0
92-93	22.316902764076055	30.0	2.0	34.0	2.0	35.0
94-95	21.697575212769312	30.0	2.0	34.0	2.0	35.0
96-97	21.066716595773705	29.0	2.0	34.0	2.0	35.0
98	20.676849034947963	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	212806.0
3	36192.0
4	26472.0
5	21346.0
6	20053.0
7	20114.0
8	23254.0
9	26449.0
10	28689.0
11	30427.0
12	31487.0
13	30965.0
14	28957.0
15	30320.0
16	32580.0
17	34939.0
18	36356.0
19	36115.0
20	37667.0
21	40042.0
22	43225.0
23	48234.0
24	54863.0
25	62939.0
26	73353.0
27	84209.0
28	97893.0
29	112278.0
30	131499.0
31	154311.0
32	183679.0
33	221389.0
34	274636.0
35	346512.0
36	433555.0
37	465833.0
38	270647.0
39	25868.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.51114073213489	17.65354812609566	13.046767216787861	24.78854392498159
2	16.07573492260198	19.21817596791138	39.16007737863714	25.5460117308495
3	19.679160941305017	23.195990984832843	28.611500411295683	28.513347662566456
4	13.542217981690827	15.135845744812816	35.811115337859626	35.51082093563673
5	14.822178516694104	36.53405111479248	33.7067399265506	14.93703044196282
6	34.31144129943682	34.106598416719066	17.035324413389787	14.546635870454322
7	30.044324697012065	31.1092854968413	20.275344438971622	18.571045367175017
8	27.19611146714076	34.10766336592727	19.587192235337895	19.109032931594072
9	27.00714235144131	15.882391243345872	19.180787121132646	37.92967928408017
10-11	26.05673470790431	24.986919121802163	27.134960814210707	21.82138535608282
12-13	26.609446496143118	22.308269534290243	27.12355980572429	23.95872416384235
14-15	23.74828344597873	23.84394148322412	24.60588539820888	27.801889672588274
16-17	23.858741406193072	26.927110976971726	25.32959184359026	23.884555773244944
18-19	23.959749992859486	26.122117122769918	26.64295456216764	23.275178322202965
20-21	24.736747789876095	25.342223994389744	26.36077641624285	23.56025179949131
22-23	24.39009092948778	25.15649014634197	26.26648553617879	24.186933387991463
24-25	24.146635575610706	25.37745822602584	26.272998017725417	24.202908180638037
26-27	23.932428153780975	25.576862238146745	26.2762709767352	24.214438631337075
28-29	24.123017015879388	25.6905704551718	26.133650841745443	24.05276168720337
30-31	24.304127449122646	25.586430947350408	25.87074386942183	24.238697734105116
32-33	24.027714012622724	25.615510522747353	25.822632955247784	24.534142509382146
34-35	24.374096594172993	25.579177752938076	25.91545987893066	24.131265773958273
36-37	24.567684523631776	25.201132673688964	25.91806422379264	24.31311857888662
38-39	24.249650384499187	25.50116746141252	25.87826943701263	24.37091271707567
40-41	24.42858390665621	25.392277704541904	26.06803923250768	24.111099156294205
42-43	24.318767880989117	25.20978501171288	25.890608496529723	24.58083861076828
44-45	24.22907808933251	25.287175050362876	25.99278053252035	24.490966327784264
46-47	24.338481610547465	25.324076896041458	25.858090152995782	24.479351340415295
48-49	24.127357646422933	25.371481199259478	25.849676033536273	24.651485120781317
50-51	24.093222759920373	25.515714541541474	25.988724375067996	24.402338323470154
52-53	24.2277268999267	25.33266744699411	26.03803726498215	24.401568388097036
54-55	23.89782498039772	25.219271128586684	26.227067795803066	24.65583609521253
56-57	23.84452752790836	25.337915534963262	26.200152569984443	24.617404367143934
58-59	23.892737326990513	25.112838689536897	26.18604645733659	24.808377526135995
60-61	23.764743209662914	25.102010876058	26.426086558354033	24.70715935592505
62-63	23.718386752176933	25.13248095414041	26.373491696182892	24.77564059749977
64-65	23.650601080842616	25.09999416111432	26.27711351442529	24.97229124361777
66-67	23.605197613767555	25.0758805098049	26.23268517595661	25.08623670047093
68-69	23.71321850888006	24.874511274918753	26.288052591384325	25.124217624816858
70-71	23.49455059065628	24.762224447053047	26.416689550270416	25.326535412020256
72-73	23.4413074131243	24.743190318980563	26.429932871237305	25.385569396657836
74-75	23.443501708499177	24.957593732206508	26.365947135096444	25.232957424197867
76-77	23.393115115054396	24.614737756749687	26.565046451289458	25.427100676906456
78-79	23.30499308461986	24.651570732913644	26.59427994179442	25.449156240672078
80-81	23.40751262892234	24.6044553725793	26.586738260699015	25.401293737799346
82-83	23.34518318224506	24.44073141977177	26.749578882078556	25.464506515904617
84-85	23.189940625528624	24.476164983385388	26.684265343806125	25.649629047279866
86-87	23.21644322658895	24.47981133205263	26.775832129489917	25.5279133118685
88-89	23.090219205697984	24.177534547244324	26.846727162539892	25.8855190845178
90-91	23.075067296953762	24.222793914138617	26.894085970278503	25.80805281862912
92-93	23.21269437013131	24.044194953366464	26.94538640187786	25.79772427462436
94-95	23.057301127609946	23.865257176916423	27.112727680000248	25.964714015473376
96-97	23.199956962819506	23.621073181355047	27.16567758929567	26.013292266529774
98	23.14184674245867	23.58754583179837	27.083011973885434	26.187595451857526
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	43.0
2	71.0
3	116.0
4	129.5
5	138.0
6	219.5
7	464.5
8	780.5
9	914.5
10	937.5
11	1078.0
12	1288.5
13	1481.0
14	1661.5
15	1815.0
16	1932.5
17	1991.5
18	1991.5
19	2018.5
20	2025.0
21	2053.0
22	2206.5
23	2451.0
24	2812.0
25	3422.0
26	4308.0
27	5483.0
28	7464.5
29	10851.5
30	15799.5
31	22213.0
32	29249.5
33	36973.0
34	46292.5
35	57194.0
36	69140.0
37	80222.0
38	89696.5
39	98900.0
40	108416.5
41	117608.5
42	127861.0
43	137997.5
44	146519.5
45	155700.5
46	164711.5
47	171860.5
48	175883.5
49	176032.5
50	175203.0
51	174762.5
52	174062.0
53	172792.0
54	168480.5
55	161110.5
56	148533.0
57	134314.0
58	122522.5
59	107814.5
60	91775.5
61	77006.5
62	62625.0
63	48145.0
64	35313.5
65	26284.5
66	19979.5
67	15227.0
68	11182.0
69	7998.5
70	5729.0
71	4154.0
72	3129.0
73	2240.0
74	1557.5
75	1068.5
76	722.5
77	465.5
78	265.5
79	137.5
80	77.0
81	59.0
82	39.5
83	26.5
84	19.5
85	16.5
86	17.5
87	14.5
88	9.0
89	6.0
90	6.5
91	8.0
92	5.0
93	2.0
94	2.0
95	3.0
96	2.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36262122902602845
2	0.022453886847913548
3	0.017441166423868403
4	0.01596835681474174
5	0.021291142419655653
6	0.021885434016320798
7	0.013642867958225951
8	0.008113372232732858
9	0.0
10-11	2.5838765072397636E-5
12-13	0.0049093653637555515
14-15	0.00306189366107912
16-17	0.005581173255637889
18-19	0.03577377024273452
20-21	0.07622435696357303
22-23	0.09551299509011786
24-25	0.06136706704694438
26-27	0.07091449074119531
28-29	0.05477818195348299
30-31	0.04860271710117996
32-33	0.018177571228431735
34-35	0.3237597263571424
36-37	0.49323618646699846
38-39	0.44830257400609896
40-41	0.45697147968788837
42-43	0.40957026516257494
44-45	0.3845712599550302
46-47	0.3829046596078606
48-49	0.37486880367034486
50-51	0.369869002628836
52-53	0.43004748648245006
54-55	0.4301766803078121
56-57	0.40126310219179906
58-59	0.38712929769719756
60-61	0.4015473286075955
62-63	0.3821165772731524
64-65	0.43096476264252015
66-67	0.44923276954870534
68-69	0.39342103699232644
70-71	0.35350014495547205
72-73	0.3401285840305063
74-75	0.3237209682095338
76-77	0.32187349650685737
78-79	0.321653867003742
80-81	0.3368212221012394
82-83	0.33232527697864217
84-85	0.3321960831532802
86-87	0.31965136271063116
88-89	0.3594947384522683
90-91	0.34059368180180943
92-93	0.33049072465850193
94-95	0.33625276926964665
96-97	0.3365240763029068
98	0.33184725982480284
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3870154.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.48483043784393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.74361668347261	41.26878111748052
2	11.84768755186038	12.910384946873341
3	4.679835283992228	7.649400957760672
4	2.453974621509339	5.348175646068343
5	1.4176252253816122	3.8619535014663713
6	0.8973514227615175	2.933522406739157
7	0.5930845328331414	2.261987714470512
8	0.3981498211736995	1.7354500396405521
9	0.3117272624155304	1.5285966332007108
>10	1.5538986639441528	14.763747886085154
>50	0.07816969389919204	2.8794670676092364
>100	0.023494645113104833	2.1472441125593327
>500	9.071462757809346E-4	0.3360167876521962
>1k	4.774453677266318E-4	0.3752711823939413
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0671012057918108E-4
2	0.0	2.5838765072397636E-5	0.0	0.0	2.0671012057918108E-4
3	0.0	2.5838765072397636E-5	0.0	0.0	2.0671012057918108E-4
4	0.0	2.5838765072397636E-5	0.0	2.5838765072397636E-5	2.0671012057918108E-4
5	0.0	1.2919382536198817E-4	0.0	2.5838765072397636E-5	2.0671012057918108E-4
6	0.0	1.2919382536198817E-4	0.0	2.5838765072397636E-5	2.5838765072397634E-4
7	0.0	1.2919382536198817E-4	0.0	2.5838765072397636E-5	2.5838765072397634E-4
8	0.0	1.2919382536198817E-4	0.0	2.5838765072397636E-5	2.5838765072397634E-4
9	0.0	1.2919382536198817E-4	0.0	7.75162952171929E-5	2.5838765072397634E-4
10-11	0.0	1.2919382536198817E-4	0.0	7.75162952171929E-5	2.5838765072397634E-4
12-13	0.0	1.2919382536198817E-4	0.0	1.0335506028959054E-4	6.718078918823386E-4
14-15	0.0	1.4211320789818697E-4	0.0	1.2919382536198817E-4	8.139210997805255E-4
16-17	0.0	1.550325904343858E-4	0.0	1.2919382536198817E-4	8.785180124615197E-4
18-19	0.0	1.550325904343858E-4	0.0	1.4211320789818697E-4	9.560343076787125E-4
20-21	0.0	1.8087135550678345E-4	0.0	1.9379073804298225E-4	0.0011627444282578936
22-23	0.0	1.9379073804298225E-4	0.0	3.488233284773681E-4	0.0011627444282578936
24-25	0.0	2.0671012057918108E-4	0.0	7.493241870995314E-4	0.0011627444282578936
26-27	0.0	2.0671012057918108E-4	0.0	0.0016795197297058463	0.0012144219584026888
28-29	0.0	2.0671012057918108E-4	0.0	0.006911869656866368	0.0012531801060112853
30-31	2.5838765072397636E-5	2.5838765072397634E-4	0.0	0.028370964049492603	0.0012790188710836829
32-33	2.5838765072397636E-5	2.5838765072397634E-4	0.0	0.07656026090951419	0.00142113207898187
34-35	2.5838765072397636E-5	2.5838765072397634E-4	0.0	0.14282377393767792	0.0014986483741990628
36-37	2.5838765072397636E-5	2.5838765072397634E-4	0.0	0.24869811382182727	0.0015503259043438582
38-39	2.5838765072397636E-5	2.5838765072397634E-4	0.0	0.4250606048234773	0.0017182778773144427
40-41	2.5838765072397636E-5	2.84226415796374E-4	0.0	0.6299103343174457	0.0017311972598506415
42-43	2.5838765072397636E-5	3.100651808687716E-4	0.0	0.8469430415430497	0.0017570360249230393
44-45	2.5838765072397636E-5	3.359039459411693E-4	0.0	1.103496139946886	0.0017957941725316358
46-47	2.5838765072397636E-5	3.359039459411693E-4	0.0	1.406701128688936	0.0018345523201402322
48-49	2.5838765072397636E-5	3.359039459411693E-4	0.0	1.7302799836905716	0.0019249879978936238
50-51	2.5838765072397636E-5	3.8758147608596456E-4	0.0	2.0822168833591634	0.001989584910574618
52-53	2.5838765072397636E-5	4.0050085862216336E-4	0.0	2.4378099682854995	0.0021704562660814016
54-55	2.5838765072397636E-5	5.296946839841515E-4	0.0	2.833233509570937	0.002196295031153799
56-57	2.5838765072397636E-5	5.942915966651456E-4	0.0	3.2971556170633	0.0022996500914433896
58-59	2.5838765072397636E-5	6.45969126809941E-4	0.0	3.789487446752765	0.002351327621588185
60-61	5.167753014479527E-5	6.45969126809941E-4	0.0	4.314453636728668	0.002364247004124384
62-63	5.167753014479527E-5	6.718078918823386E-4	0.0	4.850956835309396	0.002532198977094968
64-65	5.167753014479527E-5	7.880823347081279E-4	0.0	5.4223165279727885	0.0025709571247035648
66-67	6.459691268099408E-5	8.52679247389122E-4	0.0	6.069228769707872	0.002674312184993155
68-69	7.75162952171929E-5	8.785180124615197E-4	0.0	6.770479934390208	0.0027776672452827457
70-71	7.75162952171929E-5	9.301955426063149E-4	0.0	7.508887243246651	0.0028164253928913423
72-73	7.75162952171929E-5	9.818730727511101E-4	0.0	8.259619643042628	0.0030231355134705236
74-75	7.75162952171929E-5	9.818730727511101E-4	0.0	9.035053902247817	0.0032686037816583007
76-77	7.75162952171929E-5	9.818730727511101E-4	0.0	9.868857931751553	0.0033073619292668974
78-79	7.75162952171929E-5	9.818730727511101E-4	0.0	10.783588973462038	0.003397797607020289
80-81	7.75162952171929E-5	9.818730727511101E-4	1.2919382536198818E-5	11.720257126718987	0.0034365557546288854
82-83	7.75162952171929E-5	9.818730727511101E-4	2.5838765072397636E-5	12.686058487595067	0.0035915883450632715
84-85	7.75162952171929E-5	9.818730727511101E-4	2.5838765072397636E-5	13.656291196681064	0.0035915883450632715
86	7.75162952171929E-5	9.818730727511101E-4	2.5838765072397636E-5	14.398445126473003	0.0035915883450632715
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7190	0.0	34.246178	1
GGTATCA	4500	0.0	32.319286	1
TCAACGC	8775	0.0	28.223436	4
TATCAAC	8915	0.0	27.729132	2
ATCAACG	8950	0.0	27.466606	3
CAACGCA	9055	0.0	27.300053	5
AACGCAG	9470	0.0	26.292658	6
GTACATG	13185	0.0	25.726135	1
TACATGG	13450	0.0	24.381062	2
ACATGGG	13915	0.0	23.269375	3
ACGCAGA	11335	0.0	21.193869	7
TAAGGCG	1070	0.0	20.574093	5
GTATAGG	855	0.0	20.455244	1
CATGGGG	9750	0.0	20.226795	4
CGCAGAG	12030	0.0	19.740282	8
GAGTACA	9915	0.0	18.521126	1
CGCCGTA	625	0.0	17.66829	92
GCAGAGT	13015	0.0	17.15434	9
AGAGTAC	10105	0.0	16.808971	10-11
AGTACAT	10020	0.0	16.798372	2
>>END_MODULE