FastQCFastQC Report
Mon 15 Aug 2016
SRR2920865_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920865_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3870154
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73300.036.9092861
GGTATCA45500.035.738971
TCAACGC89300.029.8203774
TATCAAC91200.029.4050272
ATCAACG92850.028.8336073
CAACGCA92800.028.6916035
AACGCAG97950.027.4737876
GTACATG134500.025.4882681
TACATGG137800.024.179982
ACATGGG139600.023.1899973
ACGCAGA115800.022.5010997
CGCAGAG125050.020.4945668
CATGGGG97850.019.4529424
GCAGAGT133600.018.507789
GTACCGT6000.018.193516
GTATAGG7850.018.1710051
AGAGTAC105100.017.474110-11
TAAGGCG10400.017.341645
GTATTAA21350.017.1482661
TAAGGTG17250.017.0585655
Produced by FastQC (version 0.11.5)