##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920865_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3870154
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.364066649544178	31.0	30.0	34.0	27.0	34.0
2	30.375443716193207	31.0	30.0	34.0	26.0	34.0
3	30.51444722871493	31.0	30.0	34.0	27.0	34.0
4	34.16399140706029	35.0	35.0	37.0	30.0	37.0
5	33.707026645451315	35.0	35.0	37.0	28.0	37.0
6	33.65776193918898	35.0	35.0	37.0	28.0	37.0
7	33.47218896198962	35.0	35.0	37.0	28.0	37.0
8	33.40376817046557	35.0	35.0	37.0	27.0	37.0
9	34.75014172562642	38.0	35.0	39.0	27.0	39.0
10-11	34.56042963665011	38.0	34.0	39.0	27.0	39.0
12-13	34.39500495329126	37.0	34.0	39.0	27.0	39.0
14-15	35.31251740886797	38.0	34.0	40.0	27.0	41.0
16-17	35.19778618628612	38.0	34.0	40.0	26.5	41.0
18-19	35.10412802694673	38.5	34.0	40.0	26.0	41.0
20-21	34.98271412455422	38.0	34.0	40.0	25.0	41.0
22-23	34.84444856716296	38.0	34.0	40.0	25.0	41.0
24-25	34.67408596660495	38.0	34.0	40.0	24.5	41.0
26-27	34.49883402572611	38.0	33.0	40.0	24.0	41.0
28-29	34.44632965509899	38.0	33.5	40.0	24.0	41.0
30-31	34.43536006577516	38.0	33.0	40.0	24.0	41.0
32-33	34.29655473658154	38.0	33.0	40.0	23.5	41.0
34-35	34.1385851829152	38.0	33.0	40.0	23.0	41.0
36-37	33.95564259200022	38.0	33.0	40.0	22.0	41.0
38-39	33.77619714874395	38.0	33.0	40.0	21.0	41.0
40-41	33.54922271826909	38.0	32.0	40.0	19.0	41.0
42-43	33.589172420528996	38.0	33.0	40.0	19.0	41.0
44-45	33.453472781703255	38.0	32.0	40.0	18.0	41.0
46-47	33.21921659964952	38.0	32.0	40.0	16.5	41.0
48-49	32.96335662611875	37.0	32.0	40.0	15.0	41.0
50-51	32.70178240969223	37.0	31.0	40.0	13.5	41.0
52-53	32.441699348398025	37.0	31.0	40.0	10.5	41.0
54-55	32.163361716355475	36.5	31.0	40.0	8.5	41.0
56-57	31.833215293241558	36.0	30.5	39.0	8.0	41.0
58-59	31.503113312803574	36.0	30.0	39.0	7.0	40.5
60-61	31.167314789023898	35.0	29.5	39.0	4.0	40.0
62-63	30.8426689222186	35.0	29.0	39.0	2.0	40.0
64-65	30.436223984885356	35.0	29.0	38.0	2.0	40.0
66-67	30.001783779146773	34.5	28.5	38.0	2.0	40.0
68-69	29.557095660792825	34.0	28.0	37.0	2.0	39.5
70-71	29.131565694801807	34.0	27.5	36.5	2.0	39.0
72-73	28.697713450162446	34.0	26.5	36.0	2.0	39.0
74-75	28.2613753871293	33.5	26.0	35.5	2.0	38.5
76-77	26.801574691859805	31.5	25.0	34.5	2.0	36.0
78-79	27.65350965362102	33.0	26.0	35.0	2.0	37.0
80-81	27.691686558209312	33.0	26.0	35.0	2.0	36.5
82-83	27.37023294163488	33.0	26.0	35.0	2.0	36.0
84-85	27.058434496405052	33.0	25.5	35.0	2.0	36.0
86-87	26.53248617496875	33.0	25.0	35.0	2.0	35.0
88-89	26.179817521473304	32.5	24.0	35.0	2.0	35.0
90-91	25.832321530357707	32.0	23.0	35.0	2.0	35.0
92-93	25.53904715419593	32.0	22.0	35.0	2.0	35.0
94-95	25.162853338652674	32.0	19.5	34.0	2.0	35.0
96-97	24.647114300877952	32.0	16.5	34.0	2.0	35.0
98-99	24.067115546306425	32.0	2.0	34.0	2.0	35.0
100-101	21.87563686096212	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82152.0
3	37820.0
4	20386.0
5	14548.0
6	12824.0
7	12549.0
8	14346.0
9	16713.0
10	18847.0
11	20997.0
12	22705.0
13	25247.0
14	27574.0
15	29517.0
16	30648.0
17	31987.0
18	31713.0
19	30594.0
20	31289.0
21	32507.0
22	35227.0
23	40166.0
24	47181.0
25	55238.0
26	65973.0
27	78647.0
28	92617.0
29	109712.0
30	130224.0
31	153423.0
32	185437.0
33	226182.0
34	286588.0
35	373816.0
36	498533.0
37	583865.0
38	337852.0
39	24510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78460027859516	18.792488544385602	13.63664015586004	24.7862710211592
2	16.151941240581124	20.171755439189244	38.03784035467322	25.638462965556407
3	19.52288941543978	23.896721828744734	28.931832447933616	27.648556307881872
4	14.023494120526179	16.050835618424305	35.32475426065775	34.60091600039177
5	14.86157890031107	36.73855733207782	33.72728405021579	14.672579717395315
6	34.323586432023674	34.620610628774074	16.684208416159795	14.37159452304246
7	29.946349299337477	31.205953069739184	20.292742276876325	18.554955354047014
8	27.17631819342481	34.3392456097732	19.517005847081776	18.967430349720217
9	26.893837853055302	15.761773512611096	19.105429358924333	38.238959275409265
10-11	26.117183132694056	25.109869372565623	27.1060786237621	21.666868870978213
12-13	26.651583640176174	22.287047101777315	27.193307626999825	23.868061631046682
14-15	23.731205116660306	23.92995583015674	24.488038417377275	27.85080063580568
16-17	23.936167393369168	27.00177591465681	25.27942182000161	23.782634871972416
18-19	24.03625379518504	26.178585808225396	26.594921582768976	23.190238813820592
20-21	24.77259979373076	25.383963212733896	26.28459468611146	23.558842307423884
22-23	24.45056333994286	25.194726773500943	26.229524094605683	24.125185791950514
24-25	24.207131287512425	25.45901700800308	26.229463905984794	24.104387798499697
26-27	23.99249709669083	25.63492394900725	26.236748945300143	24.135830009001776
28-29	24.12660648495315	25.71906187663447	26.156724741684172	23.99760689672821
30-31	24.30154653629782	25.538550723439073	25.934598822969036	24.225303917294074
32-33	24.01816206648689	25.612344250096214	25.870657696430264	24.498835986986627
34-35	24.39134007461995	25.555336884929098	25.964993259187192	24.08832978126376
36-37	24.530605736213968	25.30791560724297	25.966700971404734	24.19477768513833
38-39	24.264959407801044	25.542612116403074	25.9145974251545	24.277831050641385
40-41	24.41773884492661	25.37112073577506	26.10523396668998	24.10590645260835
42-43	24.322506644979956	25.253172088797164	25.936528376399888	24.487792889822995
44-45	24.14229852352178	25.299387675006535	26.118776032341323	24.43953776913036
46-47	24.293621609494224	25.33931066872431	25.910515991047955	24.456551730733516
48-49	24.07714292359096	25.357470483199517	25.928606682980902	24.636779910228615
50-51	23.980305534271498	25.517344748766995	26.045355933342208	24.456993783619303
52-53	24.198639805947924	25.319550509071448	26.058357229367562	24.423452455613067
54-55	23.886219145556943	25.22893235706522	26.16275103354212	24.722097463835716
56-57	23.827809416905072	25.393542465531482	26.04719525047452	24.731452867088922
58-59	23.81709055743504	25.247076713774735	26.082257052805524	24.853575675984704
60-61	23.7655557785381	25.3088606184927	26.17194521486852	24.753638388100676
62-63	23.62079618335583	25.393128872467656	26.07515607448162	24.910918869694896
64-65	23.50595540260258	25.321511118082313	26.004489275468977	25.16804420384613
66-67	23.464001821874543	25.299408588711565	25.931726750872357	25.304862838541535
68-69	23.63441009826609	25.150207777080073	25.94327277090935	25.272109353744487
70-71	23.43766172964676	24.9972184923673	25.989567375738975	25.575552402246966
72-73	23.41371929625015	24.969509515512794	25.92811793962393	25.68865324861313
74-75	23.312985769635993	25.167838953222844	25.806532116203517	25.712643160937642
76-77	23.267783768929657	24.822379548774187	26.07708966512703	25.832747017169122
78-79	23.19366144280556	24.899348678829387	26.008627219158313	25.898362659206743
80-81	23.209074382350217	24.924457030668023	25.942434480647496	25.924034106334265
82-83	23.091921217362383	24.747406815057612	26.123349537387874	26.037322430192127
84-85	22.937736247418673	24.718118243682387	26.006668873602283	26.337476635296653
86-87	22.835511348770364	24.781166569349796	26.101148580840267	26.282173501039573
88-89	22.767451658565506	24.56115491917409	25.96398501869543	26.70740840356497
90-91	22.64727309280231	24.586051881083055	26.004662292119185	26.762012733995455
92-93	22.669581896052122	24.46772732682679	26.047697294639814	26.81499348248127
94-95	22.433008433343307	24.294090561673375	26.191683242405777	27.081217762577538
96-97	22.45988662272437	24.070985022091087	26.309835440547214	27.15929291463733
98-99	22.33231038678129	24.129790225113272	26.128638037513667	27.409261350591773
100-101	22.385542234052902	23.917606196731736	26.257711450628047	27.439140118587314
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	29.5
2	47.5
3	74.0
4	65.5
5	74.0
6	149.0
7	331.0
8	478.5
9	482.5
10	436.5
11	410.0
12	420.5
13	461.0
14	523.0
15	586.0
16	662.0
17	728.5
18	820.5
19	925.0
20	1030.0
21	1223.0
22	1454.5
23	1758.5
24	2121.0
25	2593.0
26	3293.5
27	4292.5
28	5678.5
29	8025.5
30	11820.5
31	17050.0
32	23884.0
33	30928.5
34	38024.5
35	47005.0
36	58221.0
37	69516.0
38	79526.0
39	88451.0
40	96917.0
41	105831.0
42	115481.5
43	126057.5
44	135990.0
45	145421.5
46	154476.0
47	162529.5
48	170030.5
49	174621.0
50	174304.0
51	173976.0
52	174140.5
53	173863.0
54	172323.0
55	164211.0
56	151634.5
57	136959.0
58	123865.0
59	110661.5
60	94409.0
61	79103.0
62	64644.5
63	49247.0
64	35880.5
65	26504.0
66	19856.5
67	15240.5
68	11196.0
69	7977.5
70	5664.0
71	4087.5
72	2958.5
73	2190.0
74	1616.5
75	1064.0
76	698.5
77	417.5
78	215.0
79	112.5
80	67.0
81	37.5
82	21.0
83	10.5
84	6.0
85	4.0
86	4.0
87	3.0
88	1.0
89	1.0
90	0.5
91	0.5
92	1.5
93	1.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18606494728633538
2	0.0
3	0.013617029193153555
4	0.013048576361560807
5	0.0076482744614297
6	0.10694664863465382
7	0.14278501579006933
8	0.12420694370301544
9	0.14113133482543588
10-11	0.11438821297550435
12-13	0.11472411692144549
14-15	0.11646823356383233
16-17	0.06639270685352572
18-19	0.07966091271820191
20-21	0.036794401463094234
22-23	0.08427313228362489
24-25	0.05862815794927023
26-27	0.06678028832961169
28-29	0.03971418191627517
30-31	0.023952535222112607
32-33	0.03129074450267354
34-35	0.02101983538639548
36-37	0.023564953746026643
38-39	0.0305930978457188
40-41	0.023978373987185008
42-43	0.01300981821395221
44-45	0.02709194517840892
46-47	0.034042572982883886
48-49	0.008371759883456835
50-51	0.013927094374022325
52-53	0.006847272744185374
54-55	0.011679121812723731
56-57	0.005413221282667305
58-59	0.0039145729084682414
60-61	0.02306109782711489
62-63	0.009482826781569932
64-65	0.0068343533616491755
66-67	0.041626250531632596
68-69	0.0906940654041157
70-71	0.02195003092900179
72-73	0.0448044186355375
74-75	0.08661154052267687
76-77	0.23726446027729128
78-79	0.0798676228387811
80-81	0.01724737568582542
82-83	0.011459492309608353
84-85	0.013823739313732736
86-87	0.25611383939760535
88-89	0.6173785332573329
90-91	0.26484734199207577
92-93	0.3520531741114178
94-95	0.0010335506028959056
96-97	0.003992089203685435
98-99	0.0032169262515135055
100-101	0.004612219565422978
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3870154.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.20087837867025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.09337520126226	36.19150760386408
2	13.329960868517643	13.383514887057757
3	5.5753332703212175	8.39659882271848
4	2.8042231670250284	5.630978646178918
5	1.593359336525375	3.9994019133214533
6	1.0574938097268414	3.1852270876976294
7	0.7080993734714982	2.48830473693587
8	0.48727906069479415	1.9569469489929634
9	0.3709884933833488	1.6761553412601242
>10	1.8490294123352002	16.330409605031182
>50	0.09865638438353623	3.356992658939734
>100	0.03039511223703289	2.511950710640907
>500	0.0011871352381016022	0.4131763282334424
>1k	6.193748781808702E-4	0.4788347091275672
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5838765072397636E-5
2	0.0	0.0	0.0	0.0	2.5838765072397636E-5
3	0.0	0.0	0.0	0.0	2.5838765072397636E-5
4	0.0	0.0	0.0	2.5838765072397636E-5	2.5838765072397636E-5
5	0.0	1.2919382536198817E-4	0.0	2.5838765072397636E-5	2.5838765072397636E-5
6	0.0	1.2919382536198817E-4	0.0	2.5838765072397636E-5	2.5838765072397636E-5
7	0.0	1.8087135550678345E-4	0.0	2.5838765072397636E-5	2.5838765072397636E-5
8	0.0	1.8087135550678345E-4	0.0	5.167753014479527E-5	5.167753014479527E-5
9	0.0	1.8087135550678345E-4	0.0	5.167753014479527E-5	7.75162952171929E-5
10-11	0.0	1.8087135550678345E-4	0.0	5.167753014479527E-5	1.0335506028959054E-4
12-13	0.0	1.8087135550678345E-4	0.0	5.167753014479527E-5	4.2633962369456103E-4
14-15	0.0	1.8087135550678345E-4	0.0	6.459691268099408E-5	6.072109792013445E-4
16-17	0.0	1.8087135550678345E-4	0.0	7.75162952171929E-5	7.10566039490935E-4
18-19	1.2919382536198818E-5	1.8087135550678345E-4	0.0	1.1627444282578936E-4	8.785180124615197E-4
20-21	2.5838765072397636E-5	1.8087135550678345E-4	0.0	1.9379073804298225E-4	0.0010077118378235077
22-23	2.5838765072397636E-5	1.8087135550678345E-4	0.0	3.359039459411693E-4	0.0010981475155768996
24-25	2.5838765072397636E-5	1.8087135550678345E-4	0.0	7.493241870995314E-4	0.0011110668981130984
26-27	2.5838765072397636E-5	1.8087135550678345E-4	0.0	0.001627842199561051	0.0012660994885474843
28-29	2.5838765072397636E-5	2.0671012057918108E-4	0.0	0.00688603089179397	0.0013306964012284783
30-31	2.5838765072397636E-5	2.0671012057918108E-4	0.0	0.02852599663992699	0.001356535166300876
32-33	2.5838765072397636E-5	2.0671012057918108E-4	0.0	0.0777359247203083	0.0014340514615180688
34-35	2.5838765072397636E-5	2.3254888565157872E-4	0.0	0.1445032936673838	0.0014986483741990628
36-37	2.5838765072397636E-5	3.359039459411693E-4	0.0	0.2513207484766756	0.0014986483741990628
38-39	2.5838765072397636E-5	3.617427110135669E-4	0.0	0.42981493759679845	0.0015890840519524546
40-41	2.5838765072397636E-5	3.617427110135669E-4	0.0	0.6377782382819908	0.001627842199561051
42-43	2.5838765072397636E-5	3.617427110135669E-4	0.0	0.8593068906301919	0.001627842199561051
44-45	2.5838765072397636E-5	3.875814760859645E-4	0.0	1.1206659993374941	0.0016795197297058463
46-47	2.5838765072397636E-5	4.2633962369456103E-4	0.0	1.4268812042104786	0.0018733104677488286
48-49	2.5838765072397636E-5	4.3925900623075984E-4	0.0	1.7530568551018901	0.0020283430581832145
50-51	2.5838765072397636E-5	4.3925900623075984E-4	0.0	2.104787044649903	0.0021704562660814016
52-53	2.5838765072397636E-5	4.5217838876695864E-4	0.0	2.4570727676469724	0.0022996500914433896
54-55	2.5838765072397636E-5	6.45969126809941E-4	0.0	2.8518115816579908	0.0023771663866605825
56-57	2.5838765072397636E-5	7.234854220271338E-4	0.0	3.313692426709635	0.002390085769196781
58-59	2.5838765072397636E-5	8.52679247389122E-4	0.0	3.804499769259828	0.00240300515173298
60-61	2.5838765072397636E-5	8.785180124615197E-4	0.0	4.329646830591237	0.00240300515173298
62-63	2.5838765072397636E-5	8.785180124615197E-4	0.0	4.8659303996688505	0.002532198977094968
64-65	2.5838765072397636E-5	9.301955426063149E-4	0.0	5.436217783581739	0.002583876507239764
66-67	2.5838765072397636E-5	9.301955426063149E-4	0.0	6.082794121370881	0.002596795889775963
68-69	2.5838765072397636E-5	9.301955426063149E-4	0.0	6.783929011610391	0.00262263465484836
70-71	2.5838765072397636E-5	9.431149251425137E-4	0.0	7.521599915662271	0.0026872315675293542
72-73	3.875814760859645E-5	9.689536902149113E-4	0.0	8.273792205684838	0.002855183540499939
74-75	5.167753014479527E-5	9.818730727511101E-4	0.0	9.055285655299505	0.0029456192182533304
76-77	5.167753014479527E-5	9.818730727511101E-4	0.0	9.89392153387178	0.002971457983325728
78-79	5.167753014479527E-5	0.0010335506028959053	0.0	10.814014119334786	0.003036054896006722
80-81	6.459691268099408E-5	0.0010593893679683032	1.2919382536198818E-5	11.754571006735134	0.003074813043615319
82-83	7.75162952171929E-5	0.0010593893679683032	2.5838765072397636E-5	12.72526881359243	0.003204006868977307
84-85	7.75162952171929E-5	0.0010593893679683032	2.5838765072397636E-5	13.704209186507825	0.0032427650165859035
86-87	7.75162952171929E-5	0.0010852281330407008	2.5838765072397636E-5	14.710603247312639	0.003333200694339295
88-89	7.75162952171929E-5	0.0010852281330407008	2.5838765072397636E-5	15.778041390600993	0.003410716989556488
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7330	0.0	36.909286	1
GGTATCA	4550	0.0	35.73897	1
TCAACGC	8930	0.0	29.820377	4
TATCAAC	9120	0.0	29.405027	2
ATCAACG	9285	0.0	28.833607	3
CAACGCA	9280	0.0	28.691603	5
AACGCAG	9795	0.0	27.473787	6
GTACATG	13450	0.0	25.488268	1
TACATGG	13780	0.0	24.17998	2
ACATGGG	13960	0.0	23.189997	3
ACGCAGA	11580	0.0	22.501099	7
CGCAGAG	12505	0.0	20.494566	8
CATGGGG	9785	0.0	19.452942	4
GCAGAGT	13360	0.0	18.50778	9
GTACCGT	600	0.0	18.19351	6
GTATAGG	785	0.0	18.171005	1
AGAGTAC	10510	0.0	17.4741	10-11
TAAGGCG	1040	0.0	17.34164	5
GTATTAA	2135	0.0	17.148266	1
TAAGGTG	1725	0.0	17.058565	5
>>END_MODULE