FastQCFastQC Report
Mon 15 Aug 2016
SRR2920864_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920864_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870852
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50800.037.149391
GGTATCA30800.034.2228621
TCAACGC58700.031.806594
ATCAACG58850.031.5696183
CAACGCA61150.030.682285
TATCAAC62700.029.4858362
AACGCAG64950.029.2357256
GTACATG86900.025.265631
ACGCAGA76600.024.3654887
TACATGG88250.023.6522562
CGCAGAG79000.023.3338138
ACATGGG94500.021.5532073
GTATAAT11300.019.5521431
GCAGAGT91950.018.7998189
GAGTACA66700.018.2873841
CATGGGG57950.017.8902244
AGTACAT63050.017.8261222
AGAGTAC73950.017.1142410-11
GTATAGG7350.016.9086111
TAAGGCG5600.016.3833525
Produced by FastQC (version 0.11.5)