##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920864_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2870852
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.250875349896127	31.0	28.0	31.0	16.0	34.0
2	28.27591774149277	31.0	28.0	33.0	16.0	34.0
3	28.342935476994285	31.0	28.0	34.0	16.0	34.0
4	31.784359834641425	35.0	32.0	37.0	19.0	37.0
5	31.741357269549248	35.0	32.0	37.0	19.0	37.0
6	31.86547234061526	35.0	32.0	37.0	19.0	37.0
7	31.693902367659497	35.0	32.0	37.0	18.0	37.0
8	31.62704416667944	35.0	32.0	37.0	18.0	37.0
9	32.85269564575255	37.0	32.0	39.0	17.0	39.0
10-11	32.64713053825136	37.0	32.0	39.0	17.0	39.0
12-13	32.47702877055313	37.0	32.0	39.0	17.0	39.0
14-15	33.18467409674898	38.0	32.0	40.0	16.5	41.0
16-17	33.07173776286622	38.0	32.0	40.0	16.0	41.0
18-19	32.96270619314406	38.0	32.0	40.0	11.0	41.0
20-21	32.87456754998168	38.0	32.0	40.0	10.5	41.0
22-23	32.64519278597434	38.0	31.0	40.0	10.0	41.0
24-25	32.447178224443476	37.0	31.0	40.0	10.0	41.0
26-27	32.475403294910365	37.5	31.0	40.0	9.5	41.0
28-29	32.3138014429166	37.0	31.0	40.0	8.5	41.0
30-31	32.06624967082942	37.0	30.0	40.0	8.0	41.0
32-33	31.817622434037006	37.0	30.0	40.0	7.0	41.0
34-35	31.491146182387666	37.0	30.0	40.0	2.0	41.0
36-37	31.18414846881692	37.0	30.0	40.0	2.0	41.0
38-39	30.984984074414147	36.0	29.5	40.0	2.0	41.0
40-41	30.794936137425406	36.0	29.0	39.5	2.0	41.0
42-43	30.560727616749315	36.0	28.5	39.0	2.0	40.5
44-45	30.28453277981589	36.0	28.0	39.0	2.0	40.0
46-47	30.036650443840365	35.0	27.0	39.0	2.0	40.0
48-49	29.73931519284171	35.0	27.0	39.0	2.0	40.0
50-51	28.644657230675772	34.0	25.0	38.0	2.0	39.5
52-53	29.11522415645251	34.5	26.5	38.5	2.0	39.5
54-55	29.763258607549254	35.0	28.0	39.0	2.0	40.0
56-57	29.724122838794894	35.0	27.5	39.0	2.0	40.0
58-59	29.48826080201975	35.0	27.0	39.0	2.0	40.0
60-61	29.173175942194163	35.0	26.0	39.0	2.0	40.0
62-63	28.78440372405126	34.5	26.0	38.5	2.0	40.0
64-65	28.370643627745352	34.0	25.5	38.0	2.0	40.0
66-67	27.9815873475888	34.0	25.0	38.0	2.0	40.0
68-69	27.53017902002611	34.0	23.0	37.0	2.0	39.5
70-71	27.20171311513098	33.0	23.0	36.5	2.0	39.0
72-73	26.733561500209696	33.0	22.0	36.0	2.0	39.0
74-75	26.288165150972603	33.0	20.5	35.5	2.0	38.5
76-77	25.8590050967448	32.0	19.5	35.0	2.0	37.5
78-79	25.39291175581326	32.0	18.0	35.0	2.0	37.0
80-81	24.938127426979865	32.0	14.5	35.0	2.0	36.5
82-83	24.455062295095672	31.0	7.5	35.0	2.0	36.0
84-85	24.048366303801103	31.0	4.5	34.0	2.0	35.5
86-87	23.596773362054194	31.0	2.0	34.0	2.0	35.0
88-89	23.070026772539997	30.5	2.0	34.0	2.0	35.0
90-91	22.571123659457193	30.0	2.0	34.0	2.0	35.0
92-93	22.03262167468055	30.0	2.0	34.0	2.0	35.0
94-95	21.40095919260206	29.5	2.0	34.0	2.0	35.0
96-97	20.759852127521725	29.0	2.0	34.0	2.0	35.0
98	20.36593666270501	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	163543.0
3	27373.0
4	20016.0
5	16042.0
6	14913.0
7	15026.0
8	17196.0
9	19633.0
10	21534.0
11	23513.0
12	23981.0
13	23805.0
14	22371.0
15	23559.0
16	25737.0
17	27418.0
18	28159.0
19	28045.0
20	29017.0
21	30742.0
22	33059.0
23	37162.0
24	42224.0
25	47647.0
26	55248.0
27	63636.0
28	73301.0
29	84366.0
30	97501.0
31	114198.0
32	135249.0
33	162230.0
34	199397.0
35	248761.0
36	311433.0
37	341127.0
38	203113.0
39	19576.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84276303668622	18.227465847121096	12.99115138298315	24.93861973320953
2	15.784762800012404	19.812847518250642	39.4923232850424	24.910066396694557
3	19.28805299467726	23.84279695059028	28.888495592486706	27.980654462245752
4	13.077995564372	15.89217368765403	37.05919814492366	33.97063260305031
5	14.29952603068467	36.81324271103202	34.49962860093345	14.387602657349868
6	33.23768368512684	35.24029883663038	17.54186210911655	13.980155369126232
7	29.0472799583617	31.428034924335485	21.203065459787616	18.321619657515207
8	26.443162175512768	34.44986877335308	20.29250966520658	18.81445938592757
9	26.79779382566569	15.769917780505576	19.61933251870873	37.81295587512
10-11	25.28747563441097	25.49474859728053	27.94111295183451	21.276662816473994
12-13	26.048755865707136	22.793116409140453	27.768939471789395	23.389188253363017
14-15	23.214623168537805	24.422038836760795	25.240341449710435	27.12299654499097
16-17	23.222322294932177	27.42329763518568	25.972340104236245	23.382039965645895
18-19	23.27186431062999	26.693159622059852	27.386443642187253	22.648532425122905
20-21	23.99018089936032	26.056016072816394	27.022535860824497	22.931267166998786
22-23	23.724263302926463	25.884466206777766	26.831468227065013	23.559802263230758
24-25	23.445537749221963	26.055591358760076	26.927892191187485	23.570978700830477
26-27	23.31290952552337	26.263715483082166	26.896908716820754	23.52646627457371
28-29	23.385524217790206	26.31610772756184	26.845823422293087	23.45254463235486
30-31	23.630501239961472	26.0799478923443	26.79878053030483	23.490770337389407
32-33	23.37859836063432	26.196405101526228	26.644508839178943	23.78048769866051
34-35	23.7110874885906	26.25071811064565	26.546384007134282	23.491810393629468
36-37	23.7681796598535	25.933435627328983	26.60726684083599	23.691117871981522
38-39	23.54089312980304	26.22492900838464	26.5879869171095	23.646190944702816
40-41	23.691221810911127	26.001723704151342	26.688930887021794	23.618123597915737
42-43	23.621500315909547	25.878299366134883	26.62280714793223	23.87739317002334
44-45	23.54912341616464	26.00004056073113	26.657474066482536	23.7933619566217
46-47	23.620428650824913	25.952932780670867	26.55021104216525	23.87642752633897
48-49	23.5173373335191	25.99817740050042	26.54333149022245	23.94115377575803
50-51	23.35516028642435	26.151089316982844	26.67830646524501	23.815443931347797
52-53	23.451645210592062	25.971085460138088	26.71493802452992	23.862331304739925
54-55	23.261020596781222	25.86035061893891	26.904007623306775	23.974621160973093
56-57	23.311255637890824	25.975249759831183	26.714081429031555	23.999413173246438
58-59	23.12005177864977	25.778585430226453	26.88092728820162	24.220435502922165
60-61	23.168084530468278	25.742376373770487	26.99650913841955	24.093029957341685
62-63	23.011457555540833	25.770784902956844	27.128062138667943	24.08969540283438
64-65	23.0082816415799	25.58140104358339	26.95783827955665	24.45247903528006
66-67	23.01451593772076	25.631894825493497	26.890523580744922	24.46306565604082
68-69	23.127647279438186	25.485849266913956	26.91729691405352	24.46920653959434
70-71	22.916235914513837	25.298986416516694	27.05942640679211	24.72535126217736
72-73	22.85336717500237	25.267294778668987	27.0704576139736	24.80888043235505
74-75	22.826025423112835	25.400258871810927	27.050059965446483	24.723655739629752
76-77	22.715966681378184	25.22573097208201	27.208221817568067	24.850080528971734
78-79	22.704115863673024	25.133958461170742	27.215058279880406	24.94686739527583
80-81	22.66910123374465	25.13714972517631	27.261367167035555	24.932381874043482
82-83	22.654579722822508	25.03870947562181	27.325578449136163	24.981132352419525
84-85	22.619212582134455	25.008994752725993	27.287904254941786	25.08388841019777
86-87	22.565985294436246	24.89889138695089	27.42880814487797	25.106315173734895
88-89	22.612654971046435	24.701786154375895	27.39652137739612	25.28903749718155
90-91	22.557952466473818	24.64430941371846	27.514247283312937	25.28349083649479
92-93	22.634151353434973	24.464400198291667	27.56018251639951	25.341265931873846
94-95	22.49548277513132	24.298196255508916	27.62248931418566	25.583831655174098
96-97	22.623715471104894	24.12768417303827	27.614151512810952	25.634448843045888
98	22.632742156669504	23.992245786346043	27.729072220288447	25.64593983669601
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	53.0
2	72.0
3	116.5
4	117.0
5	145.5
6	240.5
7	390.0
8	588.0
9	734.5
10	760.0
11	827.0
12	977.5
13	1095.5
14	1224.0
15	1326.5
16	1435.0
17	1488.5
18	1446.5
19	1458.0
20	1519.5
21	1597.5
22	1777.0
23	2134.0
24	2566.5
25	3198.5
26	4209.5
27	5643.0
28	7654.0
29	10682.5
30	15157.0
31	21334.0
32	28534.0
33	36247.5
34	44723.5
35	53532.5
36	62547.0
37	70926.5
38	78192.0
39	84901.0
40	91071.0
41	96721.0
42	103856.0
43	112084.5
44	118375.0
45	122473.0
46	127118.5
47	131401.0
48	132277.5
49	131135.0
50	130348.0
51	128271.5
52	124453.5
53	121535.5
54	115718.5
55	106686.5
56	96563.5
57	85393.0
58	75747.5
59	65729.5
60	54452.0
61	44193.5
62	35190.0
63	26837.5
64	19770.5
65	14493.5
66	10409.5
67	7470.5
68	5384.5
69	3859.0
70	2741.0
71	2086.5
72	1664.5
73	1230.0
74	940.5
75	678.0
76	444.0
77	307.0
78	191.0
79	106.5
80	57.0
81	38.5
82	22.0
83	12.0
84	7.5
85	8.5
86	9.5
87	5.5
88	3.5
89	2.5
90	3.5
91	4.0
92	2.0
93	2.0
94	2.5
95	2.0
96	1.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3624359597777942
2	0.023651515299290944
3	0.018322086962337312
4	0.01595345214591348
5	0.021735707727183428
6	0.02281552654055312
7	0.014560137548017105
8	0.008568884777062698
9	0.0
10-11	0.0
12-13	0.004911433957584717
14-15	0.003222040007635364
16-17	0.005625507689006609
18-19	0.035964933058200144
20-21	0.07515190612403565
22-23	0.09389198746574187
24-25	0.06175866955175676
26-27	0.07173828535918954
28-29	0.05493142802206453
30-31	0.049044673845952354
32-33	0.018618166314390294
34-35	0.3196263687574281
36-37	0.4895236675384172
38-39	0.44474601964852245
40-41	0.4546733861585341
42-43	0.4057157944749503
44-45	0.38117604111950043
46-47	0.37892932133039253
48-49	0.3710222609873306
50-51	0.3675041416276423
52-53	0.42569244252228955
54-55	0.42670259560576446
56-57	0.39726882472520353
58-59	0.382673854312239
60-61	0.39660700029120277
62-63	0.3783371626262866
64-65	0.4263542669562903
66-67	0.4465921614907352
68-69	0.38993650665377383
70-71	0.3504708706683591
72-73	0.33735629701565945
74-75	0.3206016889759556
76-77	0.31868588140384807
78-79	0.31851171707911097
80-81	0.33310668749207556
82-83	0.329954313214335
84-85	0.32957115169991347
86-87	0.316212747992582
88-89	0.35658403846662945
90-91	0.33761754350276507
92-93	0.32779467558759556
94-95	0.3337685119260763
96-97	0.33401234198070817
98	0.32882224510354413
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2870852.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.214434313143656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.47888834893946	38.366585890985164
2	11.504521998061247	12.014042163437702
3	5.3262349813204	8.343190397055658
4	2.9459305246582734	6.152803834834169
5	1.8195677835301935	4.7503851255724845
6	1.1845439525416783	3.71101754406114
7	0.8304571709989378	3.03532959835022
8	0.58331724565693	2.436606400566212
9	0.42923035461146325	2.0170818140460844
>10	1.8243883082941421	15.447999481678137
>50	0.05453859346225613	1.9148565643565947
>100	0.01777830655219335	1.5443711536664444
>500	4.685577344231137E-4	0.16688950413235648
>1k	1.3387363839578622E-4	0.09884052725765552
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.483286494740934E-5	0.0
3	0.0	0.0	0.0	3.483286494740934E-5	0.0
4	0.0	0.0	0.0	2.4383005463186539E-4	0.0
5	0.0	0.0	0.0	2.4383005463186539E-4	0.0
6	0.0	0.0	0.0	2.4383005463186539E-4	0.0
7	0.0	0.0	0.0	2.786629195792747E-4	0.0
8	0.0	0.0	0.0	2.786629195792747E-4	0.0
9	0.0	0.0	0.0	5.224929742111401E-4	0.0
10-11	0.0	0.0	0.0	6.095751365796634E-4	0.0
12-13	0.0	0.0	0.0	6.966572989481868E-4	0.0
14-15	0.0	3.483286494740934E-5	0.0	7.314901638955961E-4	0.0
16-17	0.0	1.0449859484222802E-4	0.0	8.011558937904148E-4	0.0
18-19	0.0	1.0449859484222802E-4	0.0	8.185723262641195E-4	0.0
20-21	0.0	1.2191502731593269E-4	0.0	8.359887587378242E-4	0.0
22-23	3.483286494740934E-5	1.3933145978963735E-4	0.0	9.753202185274615E-4	0.0
24-25	3.483286494740934E-5	1.3933145978963735E-4	0.0	0.0014629803277911922	1.741643247370467E-5
26-27	3.483286494740934E-5	1.741643247370467E-4	0.0	0.0026298813035294053	3.483286494740934E-5
28-29	3.483286494740934E-5	1.741643247370467E-4	0.0	0.007401983801324485	3.483286494740934E-5
30-31	3.483286494740934E-5	1.9158075721075135E-4	0.0	0.02865003141924418	3.483286494740934E-5
32-33	3.483286494740934E-5	2.0899718968445604E-4	0.0	0.07520415542145677	3.483286494740934E-5
34-35	3.483286494740934E-5	2.0899718968445604E-4	0.0	0.14081185654990225	3.483286494740934E-5
36-37	3.483286494740934E-5	2.4383005463186539E-4	0.0	0.24736559042402745	3.483286494740934E-5
38-39	3.483286494740934E-5	2.4383005463186539E-4	0.0	0.417646050719438	5.2249297421114E-5
40-41	3.483286494740934E-5	2.4383005463186539E-4	0.0	0.6139640775630371	6.966572989481868E-5
42-43	3.483286494740934E-5	2.4383005463186539E-4	0.0	0.8228916015175982	6.966572989481868E-5
44-45	3.483286494740934E-5	2.4383005463186539E-4	0.0	1.0730786540023658	6.966572989481868E-5
46-47	3.483286494740934E-5	2.4383005463186539E-4	0.0	1.3740346071479825	6.966572989481868E-5
48-49	3.483286494740934E-5	2.4383005463186539E-4	0.0	1.7021601949525786	6.966572989481868E-5
50-51	3.483286494740934E-5	2.4383005463186539E-4	0.0	2.0528923121080433	6.966572989481868E-5
52-53	3.483286494740934E-5	2.4383005463186539E-4	0.0	2.403363182776402	6.966572989481868E-5
54-55	3.483286494740934E-5	2.4383005463186539E-4	0.0	2.8032270559401877	6.966572989481868E-5
56-57	3.483286494740934E-5	2.4383005463186539E-4	0.0	3.26000783042804	6.966572989481868E-5
58-59	3.483286494740934E-5	2.4383005463186539E-4	0.0	3.7496011636963518	6.966572989481868E-5
60-61	3.483286494740934E-5	2.4383005463186539E-4	0.0	4.264343825456693	6.966572989481868E-5
62-63	3.483286494740934E-5	2.4383005463186539E-4	0.0	4.796102341743845	6.966572989481868E-5
64-65	3.483286494740934E-5	2.4383005463186539E-4	0.0	5.3663337573654095	6.966572989481868E-5
66-67	3.483286494740934E-5	2.4383005463186539E-4	0.0	6.009801271538902	6.966572989481868E-5
68-69	3.483286494740934E-5	2.4383005463186539E-4	0.0	6.715567364670836	6.966572989481868E-5
70-71	3.483286494740934E-5	2.4383005463186539E-4	0.0	7.470325882351302	6.966572989481868E-5
72-73	3.483286494740934E-5	2.4383005463186539E-4	0.0	8.227923278524981	6.966572989481868E-5
74-75	3.483286494740934E-5	2.786629195792747E-4	1.741643247370467E-5	8.99999373008431	6.966572989481868E-5
76-77	3.483286494740934E-5	2.786629195792747E-4	3.483286494740934E-5	9.840179849048297	6.966572989481868E-5
78-79	3.483286494740934E-5	2.786629195792747E-4	3.483286494740934E-5	10.75335823650958	6.966572989481868E-5
80-81	3.483286494740934E-5	2.960793520529794E-4	3.483286494740934E-5	11.705183687630013	6.966572989481868E-5
82-83	3.483286494740934E-5	3.134957845266841E-4	3.483286494740934E-5	12.66994954807841	6.966572989481868E-5
84-85	3.483286494740934E-5	3.134957845266841E-4	3.483286494740934E-5	13.625171203531217	6.966572989481868E-5
86	3.483286494740934E-5	3.134957845266841E-4	3.483286494740934E-5	14.367825300642457	6.966572989481868E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5080	0.0	37.14939	1
GGTATCA	3080	0.0	34.222862	1
TCAACGC	5870	0.0	31.80659	4
ATCAACG	5885	0.0	31.569618	3
CAACGCA	6115	0.0	30.68228	5
TATCAAC	6270	0.0	29.485836	2
AACGCAG	6495	0.0	29.235725	6
GTACATG	8690	0.0	25.26563	1
ACGCAGA	7660	0.0	24.365488	7
TACATGG	8825	0.0	23.652256	2
CGCAGAG	7900	0.0	23.333813	8
ACATGGG	9450	0.0	21.553207	3
GTATAAT	1130	0.0	19.552143	1
GCAGAGT	9195	0.0	18.799818	9
GAGTACA	6670	0.0	18.287384	1
CATGGGG	5795	0.0	17.890224	4
AGTACAT	6305	0.0	17.826122	2
AGAGTAC	7395	0.0	17.11424	10-11
GTATAGG	735	0.0	16.908611	1
TAAGGCG	560	0.0	16.383352	5
>>END_MODULE