FastQCFastQC Report
Mon 15 Aug 2016
SRR2920864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870852
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51800.038.278161
GGTATCA30950.033.799231
TCAACGC61750.030.9012154
ATCAACG61850.030.774513
CAACGCA63200.030.1150425
TATCAAC65800.029.3578782
AACGCAG66550.028.6709276
GTACATG89800.024.3041881
ACGCAGA79100.024.0619957
TAAGGCG6350.023.9184175
TACATGG90500.022.8662032
CGCAGAG82950.022.8283638
ACATGGG97400.020.95543
CATGGGG59050.018.7293424
GCAGAGT98700.018.127699
GACGGTA2453.7968148E-617.4339078
AGAGTAC79600.017.40805210-11
TATTAAG16550.016.0600242
GTATTAA19550.015.5660341
GTATAAT12000.015.4535451
Produced by FastQC (version 0.11.5)