##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920864_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2870852
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.32636652812475	31.0	30.0	34.0	27.0	34.0
2	30.336297029592608	31.0	30.0	34.0	26.0	34.0
3	30.47346084019657	31.0	30.0	34.0	27.0	34.0
4	34.125552275073744	35.0	35.0	37.0	30.0	37.0
5	33.6826402057647	35.0	35.0	37.0	28.0	37.0
6	33.633214112047575	35.0	35.0	37.0	28.0	37.0
7	33.4519456245045	35.0	35.0	37.0	28.0	37.0
8	33.38162538507732	35.0	33.0	37.0	27.0	37.0
9	34.71807672426165	38.0	35.0	39.0	27.0	39.0
10-11	34.53152635524228	37.5	34.0	39.0	27.0	39.0
12-13	34.36688550994617	37.0	34.0	39.0	27.0	39.0
14-15	35.27149100685092	38.0	34.0	40.0	27.0	41.0
16-17	35.149970635894846	38.0	34.0	40.0	26.5	41.0
18-19	35.068015174589284	38.5	34.0	40.0	26.0	41.0
20-21	34.9561523896042	38.0	34.0	40.0	25.0	41.0
22-23	34.81424939355982	38.0	34.0	40.0	25.0	41.0
24-25	34.65115930741118	38.0	33.5	40.0	24.5	41.0
26-27	34.47780676259173	38.0	33.0	40.0	24.0	41.0
28-29	34.424589808182375	38.0	33.0	40.0	24.0	41.0
30-31	34.412887881367624	38.0	33.0	40.0	24.0	41.0
32-33	34.272017679768936	38.0	33.0	40.0	24.0	41.0
34-35	34.109691826677235	38.0	33.0	40.0	23.0	41.0
36-37	33.92264752763291	38.0	33.0	40.0	22.0	41.0
38-39	33.748333944069564	38.0	33.0	40.0	20.5	41.0
40-41	33.52553910825079	38.0	32.0	40.0	19.0	41.0
42-43	33.558317008330626	38.0	32.5	40.0	19.0	41.0
44-45	33.42282848436631	38.0	32.0	40.0	18.0	41.0
46-47	33.188724636449386	38.0	32.0	40.0	15.5	41.0
48-49	32.93540698022747	37.5	31.5	40.0	15.0	41.0
50-51	32.68898745041542	37.0	31.0	40.0	13.0	41.0
52-53	32.4382853940224	37.0	31.0	40.0	9.5	41.0
54-55	32.1685652900254	37.0	31.0	40.0	8.5	41.0
56-57	31.853385510642834	36.0	30.0	39.5	8.0	41.0
58-59	31.528434241820896	36.0	30.0	39.0	7.0	41.0
60-61	31.21116466470581	35.5	29.5	39.0	2.0	40.0
62-63	30.894142923424823	35.0	29.0	39.0	2.0	40.0
64-65	30.488504283745733	35.0	29.0	38.5	2.0	40.0
66-67	30.05136715511632	34.5	28.0	38.0	2.0	40.0
68-69	29.602881479087046	34.0	28.0	37.5	2.0	40.0
70-71	29.171767649464343	34.0	27.5	37.0	2.0	39.0
72-73	28.729927039081083	34.0	26.5	36.0	2.0	39.0
74-75	28.275446278665704	33.5	26.0	36.0	2.0	39.0
76-77	26.81657692559561	31.5	25.0	34.5	2.0	36.5
78-79	27.65716727995731	33.0	26.0	35.0	2.0	37.0
80-81	27.676228346149507	33.0	26.0	35.0	2.0	37.0
82-83	27.329611557823252	33.0	26.0	35.0	2.0	36.0
84-85	27.001491543277048	33.0	25.5	35.0	2.0	36.0
86-87	26.454185203556296	33.0	25.0	35.0	2.0	35.5
88-89	26.0902763360842	32.0	24.0	35.0	2.0	35.0
90-91	25.723826933607164	32.0	22.5	35.0	2.0	35.0
92-93	25.417093775645696	32.0	20.0	34.0	2.0	35.0
94-95	25.02993153252066	32.0	19.0	34.0	2.0	35.0
96-97	24.498423987025454	32.0	12.0	34.0	2.0	35.0
98-99	23.898715781935117	32.0	2.0	34.0	2.0	35.0
100-101	21.70171799173207	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59196.0
3	27625.0
4	14922.0
5	10554.0
6	8848.0
7	9165.0
8	10046.0
9	12189.0
10	13961.0
11	15811.0
12	17745.0
13	19481.0
14	20954.0
15	22832.0
16	24016.0
17	25056.0
18	24323.0
19	23378.0
20	23810.0
21	24840.0
22	26872.0
23	30435.0
24	35415.0
25	42251.0
26	49422.0
27	59031.0
28	70123.0
29	82321.0
30	96908.0
31	115328.0
32	137657.0
33	167801.0
34	209172.0
35	270016.0
36	359539.0
37	431907.0
38	259280.0
39	18620.0
40	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.96113370157416	19.588897776177134	13.706676214685242	24.743292307563454
2	15.882985260124869	20.949878293969874	38.41218565081028	24.754950795094977
3	19.070578251436327	24.772236071842137	29.311245782988145	26.84593989373339
4	13.560674205058278	16.97883476597147	36.543420536485065	32.91707049248519
5	14.218383088831846	37.13424492043846	34.55366358596997	14.093708404759722
6	33.05618126407845	35.89847482094663	17.299345156318648	13.745998758656281
7	28.933913298265683	31.567998214061475	21.27795761186533	18.22013087580751
8	26.26376197901173	34.997006009951136	20.1593004979056	18.57993151313153
9	26.544016441418027	15.818900236003552	19.638348798321257	37.99873452425716
10-11	25.31103540143949	25.70788930424594	27.971618172180996	21.009457122133572
12-13	26.061975864256233	22.93533800816474	27.76492774515578	23.237758382423248
14-15	23.15778883748049	24.611304376936243	25.228582946142996	27.00232383944027
16-17	23.288432317090066	27.624050000444406	25.889377693978194	23.198139988487334
18-19	23.26634066216005	26.826995208932914	27.346399309506282	22.560264819400754
20-21	24.06551052344931	26.10371558723534	26.99080676932319	22.839967119992167
22-23	23.736336178466697	26.007103304696074	26.812500087152834	23.444060429684395
24-25	23.471592492277257	26.215899393768936	26.943589266387054	23.368918847566754
26-27	23.355639339108333	26.411616736370757	26.89627480757454	23.336469116946372
28-29	23.444377772016537	26.361406839336638	26.912917910676605	23.281297477970224
30-31	23.634140138040603	26.218168261810277	26.791862502917947	23.355829097231172
32-33	23.436469390023497	26.26629681288574	26.621040062188957	23.67619373490181
34-35	23.71365327434741	26.344346101673715	26.62351654772106	23.318484076257814
36-37	23.83763640934152	26.076610994693528	26.585325531487534	23.500427064477417
38-39	23.5438975371051	26.26111689517191	26.647133266154604	23.547852301568387
40-41	23.686750744954303	26.064315621959537	26.743950866551053	23.50498276653511
42-43	23.648167163534353	25.965530185256313	26.629303610107357	23.756999041101974
44-45	23.48570673000455	26.123079973081165	26.700061443648753	23.69115185326553
46-47	23.628321636776885	25.9891955665864	26.617572605353505	23.76491019128321
48-49	23.45892328725177	26.040336187732276	26.64101231851128	23.85972820650467
50-51	23.351063570355063	26.182333214651766	26.643379763143173	23.823223451850005
52-53	23.49076237873298	26.019327479783094	26.707718503580775	23.782191637903153
54-55	23.3393253103548	25.950294460382406	26.72289370685646	23.987486522406336
56-57	23.338305949112115	26.080333435224055	26.489951571424964	24.091409044238862
58-59	23.105538027182863	25.94064079240824	26.70218593273343	24.25163524767547
60-61	23.179486071731436	26.01189601025153	26.721374702721683	24.08724321529535
62-63	22.97928093337609	26.057730376404535	26.784607299912892	24.178381390306487
64-65	22.869245170986925	25.86843333210829	26.62807137145382	24.634250125450965
66-67	22.928524149846005	25.92936794877759	26.550430445478096	24.59167745589831
68-69	23.108725069940046	25.80077846162052	26.517200919901846	24.573295548537583
70-71	22.872804003412245	25.63561067732218	26.584255769921207	24.90732954934437
72-73	22.85426394246233	25.60843146144472	26.573067008917167	24.96423758717578
74-75	22.809224844873803	25.714291690590564	26.452106366074467	25.024377098461166
76-77	22.72711402469298	25.57411769142086	26.694788023186067	25.003980260700093
78-79	22.719683467908858	25.558972839420097	26.60994304688283	25.111400645788212
80-81	22.65291284036905	25.596602815588337	26.595931475203432	25.15455286883918
82-83	22.60159344506725	25.50630375786881	26.61929413240156	25.272808664662378
84-85	22.531870557469773	25.474899045577242	26.54975468375743	25.44347571319555
86-87	22.4527147505673	25.46599044971012	26.619861585750414	25.461433213972168
88-89	22.481410727509317	25.32136302289941	26.551477486627324	25.645748762963954
90-91	22.339178120942837	25.270192281667363	26.638227058779364	25.752402538610436
92-93	22.376327984693003	25.165279347788854	26.596098309840844	25.8622943576773
94-95	22.143611406274193	25.06369688000673	26.69883745900784	26.093854254711236
96-97	22.239709008725626	24.937185163820185	26.688511216101496	26.134594611352686
98-99	22.14773937754419	24.860872981978975	26.722820244687988	26.26856739578885
100-101	22.191730383404686	24.720596464955488	26.769320588766522	26.318352562873304
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	73.0
1	51.5
2	50.0
3	74.5
4	65.0
5	72.0
6	132.0
7	260.5
8	391.5
9	421.5
10	352.0
11	322.5
12	338.5
13	353.5
14	399.5
15	459.0
16	502.0
17	558.0
18	659.0
19	724.5
20	807.5
21	948.5
22	1191.5
23	1520.5
24	1903.0
25	2386.0
26	3224.0
27	4454.0
28	5989.0
29	8370.0
30	11763.5
31	16486.5
32	23032.0
33	30322.0
34	37607.0
35	45578.5
36	55351.0
37	64046.0
38	71058.5
39	78553.5
40	85358.5
41	91188.5
42	96436.5
43	103566.5
44	111569.5
45	118164.5
46	123003.0
47	126671.5
48	130150.5
49	132079.5
50	131032.0
51	127240.5
52	123699.0
53	121521.0
54	116722.0
55	107572.5
56	96328.5
57	85310.5
58	75711.5
59	65929.5
60	54955.0
61	44487.0
62	35465.5
63	26464.0
64	19078.0
65	14132.0
66	10203.0
67	7237.0
68	5113.5
69	3709.0
70	2696.5
71	1974.5
72	1514.0
73	1208.0
74	872.5
75	612.5
76	443.5
77	287.0
78	168.0
79	72.5
80	34.5
81	25.0
82	13.0
83	7.0
84	2.5
85	2.0
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18586816735937625
2	0.0
3	0.01170384262232954
4	0.013584817329489643
5	0.0063047485554810915
6	0.10421993192264874
7	0.13974945416900628
8	0.11985988828403554
9	0.13745048508247726
10-11	0.11048984761318242
12-13	0.10989768890907647
14-15	0.11090784199255133
16-17	0.06400538934086467
18-19	0.07593564558535236
20-21	0.03563402084119975
22-23	0.08109090959756896
24-25	0.05456568294011674
26-27	0.0633609813393376
28-29	0.038316151442150274
30-31	0.023756013894133173
32-33	0.0309664169382469
34-35	0.019593486532917755
36-37	0.023564433136922417
38-39	0.03121024699287877
40-41	0.024713917680186926
42-43	0.012870743598067752
44-45	0.025863402223451435
46-47	0.032011402886669184
48-49	0.008830131264168269
50-51	0.014838800467596381
52-53	0.006008669203428111
54-55	0.012087004136751041
56-57	0.00451085601068951
58-59	0.0038141987117413225
60-61	0.022502030756026435
62-63	0.009875117212590549
64-65	0.007262652341534848
66-67	0.03805490495504471
68-69	0.08716924453089188
70-71	0.02091713540091931
72-73	0.04246126237089198
74-75	0.08293705143978164
76-77	0.23400718671669593
78-79	0.07887902267340846
80-81	0.016545610850019436
82-83	0.011912839812013994
84-85	0.01301007505785739
86-87	0.2533742596274556
88-89	0.6081469891168196
90-91	0.26035824904941113
92-93	0.34616901184735405
94-95	9.927366510011663E-4
96-97	0.003936113739057255
98-99	0.003274289305056478
100-101	0.004824351795216194
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2870852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.28246328225954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.89543059604571	32.57545667472993
2	13.187108113021582	12.470379103062596
3	6.232400024727116	8.840496759885399
4	3.4759738306215064	6.574104200658256
5	2.1455115991114804	5.072253670332526
6	1.403493202432215	3.981636948654124
7	0.9872792198280491	3.26766954226003
8	0.7429527319840936	2.8102908216393856
9	0.5342794505163234	2.2735843651353482
>10	2.300801830058624	17.687005938115387
>50	0.07089878166188864	2.2413383787504633
>100	0.02298290849877196	1.815048212262801
>500	5.918076617720819E-4	0.20287039927011535
>1k	2.9590383086121514E-4	0.18786498524367842
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3933145978963735E-4	0.0
5	0.0	0.0	0.0	1.741643247370467E-4	0.0
6	0.0	0.0	0.0	1.741643247370467E-4	0.0
7	0.0	0.0	0.0	1.741643247370467E-4	0.0
8	0.0	0.0	0.0	1.741643247370467E-4	0.0
9	0.0	0.0	0.0	2.786629195792747E-4	0.0
10-11	0.0	0.0	0.0	3.3091221700038873E-4	0.0
12-13	0.0	0.0	0.0	3.8316151442150276E-4	0.0
14-15	0.0	0.0	0.0	4.8766010926373077E-4	0.0
16-17	0.0	0.0	0.0	6.269915690533681E-4	0.0
18-19	0.0	0.0	0.0	6.269915690533681E-4	0.0
20-21	0.0	0.0	0.0	6.269915690533681E-4	0.0
22-23	0.0	0.0	0.0	7.837394613167102E-4	0.0
24-25	0.0	0.0	0.0	0.0012888160030541456	0.0
26-27	0.0	0.0	0.0	0.00264729773600311	0.0
28-29	0.0	0.0	0.0	0.007837394613167101	0.0
30-31	0.0	0.0	0.0	0.028719697149139002	0.0
32-33	0.0	0.0	0.0	0.07590081272040496	0.0
34-35	0.0	0.0	0.0	0.14185684249832453	0.0
36-37	0.0	0.0	0.0	0.24954264448324054	0.0
38-39	0.0	0.0	0.0	0.4226968161368123	0.0
40-41	3.483286494740934E-5	0.0	0.0	0.6224458801777313	0.0
42-43	5.2249297421114E-5	0.0	0.0	0.834874107059507	0.0
44-45	6.966572989481868E-5	0.0	0.0	1.0899900099343331	0.0
46-47	6.966572989481868E-5	0.0	0.0	1.3957354820102186	0.0
48-49	6.966572989481868E-5	0.0	0.0	1.725184718682816	0.0
50-51	6.966572989481868E-5	0.0	0.0	2.074941515619753	0.0
52-53	6.966572989481868E-5	0.0	0.0	2.425203389098428	0.0
54-55	6.966572989481868E-5	0.0	0.0	2.822907624635474	0.0
56-57	6.966572989481868E-5	0.0	0.0	3.279131073284168	0.0
58-59	6.966572989481868E-5	0.0	0.0	3.7687418229849534	0.0
60-61	6.966572989481868E-5	0.0	0.0	4.283519317610242	0.0
62-63	6.966572989481868E-5	0.0	0.0	4.8150514202752355	0.0
64-65	6.966572989481868E-5	0.0	0.0	5.383941770596325	0.0
66-67	6.966572989481868E-5	0.0	0.0	6.024883902061131	0.0
68-69	6.966572989481868E-5	0.0	0.0	6.729570176379696	0.0
70-71	6.966572989481868E-5	0.0	0.0	7.482621883677737	0.0
72-73	6.966572989481868E-5	0.0	0.0	8.24131651509726	0.0
74-75	6.966572989481868E-5	0.0	0.0	9.016225845149801	0.0
76-77	6.966572989481868E-5	0.0	0.0	9.861671726720846	0.0
78-79	6.966572989481868E-5	0.0	0.0	10.777096833971239	0.0
80-81	6.966572989481868E-5	0.0	0.0	11.730333016122042	0.0
82-83	6.966572989481868E-5	0.0	0.0	12.698024837226022	0.0
84-85	6.966572989481868E-5	0.0	0.0	13.65941190977452	0.0
86-87	6.966572989481868E-5	0.0	0.0	14.67205554309313	0.0
88-89	1.0449859484222802E-4	0.0	0.0	15.746057268016603	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5180	0.0	38.27816	1
GGTATCA	3095	0.0	33.79923	1
TCAACGC	6175	0.0	30.901215	4
ATCAACG	6185	0.0	30.77451	3
CAACGCA	6320	0.0	30.115042	5
TATCAAC	6580	0.0	29.357878	2
AACGCAG	6655	0.0	28.670927	6
GTACATG	8980	0.0	24.304188	1
ACGCAGA	7910	0.0	24.061995	7
TAAGGCG	635	0.0	23.918417	5
TACATGG	9050	0.0	22.866203	2
CGCAGAG	8295	0.0	22.828363	8
ACATGGG	9740	0.0	20.9554	3
CATGGGG	5905	0.0	18.729342	4
GCAGAGT	9870	0.0	18.12769	9
GACGGTA	245	3.7968148E-6	17.433907	8
AGAGTAC	7960	0.0	17.408052	10-11
TATTAAG	1655	0.0	16.060024	2
GTATTAA	1955	0.0	15.566034	1
GTATAAT	1200	0.0	15.453545	1
>>END_MODULE