FastQCFastQC Report
Mon 15 Aug 2016
SRR2920863_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920863_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298868
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46700.041.5890161
GGTATCA24600.037.9790421
TCAACGC52600.035.496594
CAACGCA53150.035.1292655
ATCAACG54850.034.207763
AACGCAG58250.032.2849436
TATCAAC59400.031.7438532
ACGCAGA68750.027.1486477
GTACATG85000.025.8275091
CGCAGAG72700.025.292428
TACATGG85350.024.8337752
ACATGGG87150.023.529793
GCAGAGT82150.021.154319
CATGGGG58850.020.657484
GTATAGC10550.019.1947861
GAGTACA61900.018.216231
AGAGTAC73150.017.36489710-11
GTATTAG10300.016.5328451
CAGAGTA77550.016.46835110-11
GTATAGA9400.016.1573141
Produced by FastQC (version 0.11.5)