##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920863_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3298868
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.454425578713668	31.0	28.0	31.0	20.0	34.0
2	28.484682018195333	31.0	28.0	33.0	16.0	34.0
3	28.556986517799437	31.0	28.0	34.0	18.0	34.0
4	32.019745864338915	35.0	32.0	37.0	22.0	37.0
5	31.978576893649578	35.0	32.0	37.0	22.0	37.0
6	32.10312204065152	35.0	32.0	37.0	23.0	37.0
7	31.934019791031346	35.0	32.0	37.0	21.0	37.0
8	31.875581866264426	35.0	32.0	37.0	19.0	37.0
9	33.118017453259725	37.0	32.0	39.0	19.0	39.0
10-11	32.92278987822489	37.0	32.0	39.0	18.0	39.0
12-13	32.754849693895	37.0	32.0	39.0	17.0	39.0
14-15	33.49622082484052	38.0	32.0	40.0	17.0	41.0
16-17	33.38357976129994	38.0	32.0	40.0	17.0	41.0
18-19	33.27300061718141	38.0	32.0	40.0	17.0	41.0
20-21	33.18402721782138	38.0	32.0	40.0	16.0	41.0
22-23	32.9666484078781	38.0	32.0	40.0	13.0	41.0
24-25	32.787795692340524	38.0	31.5	40.0	10.5	41.0
26-27	32.81754529129387	38.0	32.0	40.0	10.0	41.0
28-29	32.659349207061325	38.0	31.0	40.0	10.0	41.0
30-31	32.420253250508964	37.0	31.0	40.0	9.0	41.0
32-33	32.183382602759494	37.0	30.0	40.0	8.5	41.0
34-35	31.86204146392035	37.0	30.0	40.0	5.0	41.0
36-37	31.566178458792532	37.0	30.0	40.0	2.0	41.0
38-39	31.369961605011174	37.0	30.0	40.0	2.0	41.0
40-41	31.18051813531187	36.5	30.0	40.0	2.0	41.0
42-43	30.957606215222917	36.0	29.0	39.5	2.0	41.0
44-45	30.685069090366756	36.0	29.0	39.0	2.0	40.0
46-47	30.456976605308242	36.0	28.0	39.0	2.0	40.0
48-49	30.16352124425712	35.5	28.0	39.0	2.0	40.0
50-51	29.05379512002299	34.0	25.5	38.0	2.0	39.5
52-53	29.524053705695408	34.5	27.5	38.5	2.0	39.5
54-55	30.188255941128894	36.0	28.0	39.0	2.0	40.0
56-57	30.153240141769842	36.0	28.0	39.0	2.0	41.0
58-59	29.928202037789934	35.0	28.0	39.0	2.0	40.5
60-61	29.610277070801256	35.0	27.5	39.0	2.0	40.0
62-63	29.23048209264511	35.0	26.5	39.0	2.0	40.0
64-65	28.818981693114125	34.0	26.0	38.0	2.0	40.0
66-67	28.445097833559878	34.0	26.0	38.0	2.0	40.0
68-69	28.000360881369005	34.0	25.5	37.0	2.0	40.0
70-71	27.67583364960344	34.0	25.0	37.0	2.0	39.0
72-73	27.215081052045733	33.0	24.0	36.0	2.0	39.0
74-75	26.772499687771685	33.0	22.5	36.0	2.0	39.0
76-77	26.345043815029882	33.0	21.5	35.0	2.0	37.5
78-79	25.879928357242544	32.0	20.0	35.0	2.0	37.0
80-81	25.42335416270066	32.0	18.5	35.0	2.0	36.5
82-83	24.94705138247423	32.0	17.0	35.0	2.0	36.0
84-85	24.547904917686914	31.0	10.5	34.5	2.0	36.0
86-87	24.092467325155177	31.0	6.0	34.0	2.0	35.0
88-89	23.56447408626232	31.0	2.0	34.0	2.0	35.0
90-91	23.06915281241929	31.0	2.0	34.0	2.0	35.0
92-93	22.536826723591243	30.0	2.0	34.0	2.0	35.0
94-95	21.90798692157431	30.0	2.0	34.0	2.0	35.0
96-97	21.27378179423972	29.0	2.0	34.0	2.0	35.0
98	20.882008313154696	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	172028.0
3	29336.0
4	21585.0
5	17377.0
6	16079.0
7	16192.0
8	18631.0
9	21439.0
10	23485.0
11	25114.0
12	26212.0
13	25484.0
14	24589.0
15	25911.0
16	27858.0
17	29731.0
18	30857.0
19	30635.0
20	32206.0
21	34071.0
22	37349.0
23	41622.0
24	47319.0
25	54113.0
26	62372.0
27	71556.0
28	83395.0
29	96175.0
30	111390.0
31	130826.0
32	155488.0
33	186995.0
34	232256.0
35	291066.0
36	364124.0
37	406332.0
38	251571.0
39	26096.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.29148021489223	18.02169913810072	12.63016638358549	25.056654263421564
2	15.968072768618532	19.600318362706084	39.31988629903354	25.11172256964184
3	19.511403880839474	23.42847406260242	29.09619995955501	27.963922097003096
4	13.186354421115535	15.413746173508445	36.79359032157233	34.60630908380369
5	14.212930327465006	36.76503805846495	34.63597733925775	14.386054274812285
6	33.66246903930193	35.00571383870814	17.260431886767453	14.071385235222472
7	29.072471000412627	31.46413922591164	21.082497652656173	18.380892121019556
8	26.353285422127314	34.6933034055033	20.066743426457634	18.886667745911755
9	26.415152106722672	15.913580052308854	19.63885793551	38.03240990545848
10-11	25.450324997897006	25.39312354921803	27.96461209793967	21.191939354945298
12-13	25.99368479831643	22.766920493393318	27.673903744709673	23.56549096358058
14-15	23.00851314986545	24.45929236241652	25.210236878554156	27.321957609163878
16-17	23.29065892058018	27.417271905460883	25.965380886640727	23.32668828731821
18-19	23.23008275945931	26.79110631852999	27.216995414516738	22.76181550749396
20-21	23.91745606270109	26.159248780943585	27.05119096517961	22.872104191175712
22-23	23.47946477754868	26.03275361874708	26.913847778763717	23.573933824940525
24-25	23.281109359443043	26.16412750626923	27.002334799123105	23.55242833516462
26-27	23.21157647000296	26.35766696035456	26.90705392416118	23.523702645481297
28-29	23.27717526881639	26.57596048639824	26.78848106323981	23.35838318154556
30-31	23.570093375746204	26.3425995357674	26.666367429171316	23.420939659315078
32-33	23.255364459436763	26.342577741988098	26.656892891511397	23.74516490706374
34-35	23.61908946877062	26.355168607320863	26.680280135384578	23.345461788523934
36-37	23.624562543920756	26.11321511065975	26.70619674489118	23.55602560052831
38-39	23.490504237770008	26.30690519096188	26.740250343030027	23.462340228238087
40-41	23.66441420489693	26.088412841940094	26.834567764396038	23.412605188766932
42-43	23.557690478599998	25.993080353576126	26.677664242685257	23.771564925138616
44-45	23.457900386928415	26.073516399119278	26.78091745486545	23.687665759086855
46-47	23.45648640618104	25.991555904667013	26.666526697517696	23.88543099163426
48-49	23.31013749403091	26.06476447904289	26.72023468958229	23.90486333734391
50-51	23.242461585923415	26.242265197194214	26.857276810902604	23.65799640597977
52-53	23.396066106554795	26.040087806805545	26.803283105104843	23.760562981534818
54-55	23.102539100850162	25.938452368304333	27.01213704027181	23.94687149057369
56-57	23.034701312945746	26.130287835238036	26.911880330708392	23.923130521107822
58-59	23.073509156218424	25.834014566756082	27.027979789542616	24.064496487482877
60-61	23.029207693217195	25.821242398608934	27.18406417618443	23.965485731989443
62-63	22.989698908974066	25.806935025291743	27.190328281475928	24.013037784258263
64-65	22.826875314602855	25.689340802469975	27.12672290488388	24.35706097804329
66-67	22.818007959733045	25.774164878096606	27.032583387353277	24.375243774817076
68-69	22.944435577091475	25.53881341833211	27.08983771425372	24.426913290322698
70-71	22.698413374390896	25.37235160613941	27.238345485224418	24.690889534245276
72-73	22.705526621501257	25.316556566536608	27.257982861394886	24.71993395056725
74-75	22.657202560070942	25.391201774588406	27.192535513587128	24.759060151753523
76-77	22.600851219629384	25.25744589105289	27.345202523492773	24.796500365824954
78-79	22.547251399889433	25.22212430446462	27.3671921991781	24.863432096467843
80-81	22.598919973047227	25.145427723185726	27.36903141098917	24.886620892777877
82-83	22.590142785064515	25.057791927164992	27.449120142429233	24.90294514534126
84-85	22.525593896240654	24.96413243059331	27.45925314862897	25.051020524537066
86-87	22.40897516967639	24.923358834522134	27.5564882222032	25.111177773598282
88-89	22.50003232216913	24.673329343129865	27.5512952824083	25.27534305229271
90-91	22.402667515041728	24.68479439780477	27.666906742209356	25.245631344944147
92-93	22.54137478371555	24.470923265265284	27.663303787827065	25.324398163192104
94-95	22.416826351576063	24.331646231408257	27.76692597944851	25.484601437567168
96-97	22.48527071710288	24.106693171062844	27.834755876506385	25.573280235327893
98	22.44576337577216	24.006259990316845	27.78478941567747	25.76318721823353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	37.0
2	63.5
3	105.0
4	122.5
5	144.5
6	236.0
7	422.0
8	669.5
9	830.5
10	858.0
11	929.5
12	1085.5
13	1210.5
14	1366.5
15	1562.0
16	1629.0
17	1674.5
18	1711.0
19	1708.0
20	1742.5
21	1830.5
22	2056.0
23	2401.5
24	2907.0
25	3760.5
26	5175.0
27	7304.0
28	10443.5
29	14795.0
30	20814.0
31	28706.0
32	36233.5
33	43338.5
34	52291.5
35	62458.5
36	72899.5
37	82351.5
38	90224.5
39	97688.5
40	105295.0
41	112646.0
42	120289.0
43	127980.5
44	134990.0
45	141694.0
46	146472.5
47	148243.5
48	148916.0
49	147457.0
50	145531.0
51	144447.5
52	140853.0
53	136407.5
54	130231.0
55	121274.0
56	109632.0
57	97742.5
58	86777.5
59	73825.0
60	60537.5
61	48833.5
62	39630.5
63	30877.5
64	22560.5
65	16883.0
66	12775.0
67	9713.0
68	7327.5
69	5305.0
70	3794.5
71	2745.0
72	2072.5
73	1524.0
74	1100.0
75	806.0
76	537.0
77	327.0
78	184.0
79	104.0
80	60.5
81	40.0
82	35.0
83	26.5
84	14.0
85	15.0
86	11.0
87	6.5
88	5.5
89	5.0
90	6.5
91	6.0
92	4.5
93	4.0
94	3.0
95	0.5
96	1.0
97	2.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.358456294704729
2	0.022522877544660775
3	0.017005833516224353
4	0.014580759218010542
5	0.019794668959170238
6	0.022280370114839396
7	0.013913863786001743
8	0.008063372041560924
9	0.0
10-11	1.5156714363836321E-5
12-13	0.004880462025155296
14-15	0.0026978951567628653
16-17	0.00515328288370435
18-19	0.03572437575556221
20-21	0.07475291524244074
22-23	0.09335020376686791
24-25	0.06094514845698585
26-27	0.06925102792836815
28-29	0.05382149270598277
30-31	0.04845601582118472
32-33	0.01776366923441617
34-35	0.31701783763400054
36-37	0.48401451649474914
38-39	0.4405905298423581
40-41	0.44959361817447685
42-43	0.40303219164877163
44-45	0.37806908309153325
46-47	0.37678076237060715
48-49	0.36811112175449273
50-51	0.36435225659226134
52-53	0.4222357487477522
54-55	0.4233270321819485
56-57	0.3944534913188403
58-59	0.38041837381792787
60-61	0.39524164046575977
62-63	0.37649278479769427
64-65	0.423554382897406
66-67	0.4421819848505608
68-69	0.3874510892827479
70-71	0.34711907236057943
72-73	0.33428133529441006
74-75	0.31657829291744927
76-77	0.31556279305507223
78-79	0.3150777781954295
80-81	0.3302496492736296
82-83	0.32674844825558347
84-85	0.3252782469622913
86-87	0.3130922486137669
88-89	0.35316660139175016
90-91	0.3338721040065865
92-93	0.3232472472375372
94-95	0.3298858881288975
96-97	0.3292038359825249
98	0.3238686725264545
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3298868.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.081915027058706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.95936279966868	44.87824618439549
2	12.332446843878692	14.572491530115183
3	4.863783043635309	8.62084849482331
4	2.426618166860324	5.734769933502346
5	1.3578428788727945	4.011197879482961
6	0.7647700604437431	2.711044783582547
7	0.5319302244731041	2.199921942585094
8	0.3615346612357848	1.7088128107575276
9	0.2626849682221059	1.3967937874245668
>10	1.0813542721665261	10.546070601687196
>50	0.04195195475714901	1.6917711511843143
>100	0.014842665953792442	1.485940560445281
>500	6.709986955917692E-4	0.28994929687036114
>1k	2.0646113634614394E-4	0.15214104314377588
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.0313428727672646E-5
2	0.0	0.0	0.0	0.0	3.0313428727672646E-5
3	0.0	0.0	0.0	0.0	3.0313428727672646E-5
4	0.0	0.0	0.0	0.0	3.0313428727672646E-5
5	3.0313428727672646E-5	0.0	0.0	3.0313428727672646E-5	3.0313428727672646E-5
6	3.0313428727672646E-5	0.0	0.0	3.0313428727672646E-5	3.0313428727672646E-5
7	3.0313428727672646E-5	0.0	3.0313428727672646E-5	3.0313428727672646E-5	3.0313428727672646E-5
8	3.0313428727672646E-5	0.0	3.0313428727672646E-5	3.0313428727672646E-5	3.0313428727672646E-5
9	3.0313428727672646E-5	0.0	3.0313428727672646E-5	3.0313428727672646E-5	3.0313428727672646E-5
10-11	3.0313428727672646E-5	0.0	3.0313428727672646E-5	3.0313428727672646E-5	3.0313428727672646E-5
12-13	3.0313428727672646E-5	0.0	3.0313428727672646E-5	9.094028618301794E-5	1.2125371491069058E-4
14-15	3.0313428727672646E-5	3.0313428727672646E-5	3.0313428727672646E-5	1.0609700054685427E-4	1.2125371491069058E-4
16-17	3.0313428727672646E-5	3.0313428727672646E-5	3.0313428727672646E-5	1.2125371491069058E-4	1.2125371491069058E-4
18-19	3.0313428727672646E-5	3.0313428727672646E-5	3.0313428727672646E-5	2.576641441852175E-4	1.6672385800219953E-4
20-21	3.0313428727672646E-5	3.0313428727672646E-5	3.0313428727672646E-5	3.486044303682354E-4	2.121940010937085E-4
22-23	3.0313428727672646E-5	3.0313428727672646E-5	3.0313428727672646E-5	4.69858145278926E-4	2.121940010937085E-4
24-25	4.547014309150897E-5	4.547014309150897E-5	3.0313428727672646E-5	8.94246147466343E-4	2.121940010937085E-4
26-27	6.062685745534529E-5	6.062685745534529E-5	3.0313428727672646E-5	0.0018491191523880314	2.576641441852175E-4
28-29	6.062685745534529E-5	6.062685745534529E-5	3.0313428727672646E-5	0.007578357181918161	2.728208585490538E-4
30-31	6.062685745534529E-5	7.578357181918161E-5	3.0313428727672646E-5	0.03266271945406728	2.879775729128901E-4
32-33	6.062685745534529E-5	9.094028618301794E-5	3.0313428727672646E-5	0.0856657495844029	3.3344771600439906E-4
34-35	6.062685745534529E-5	1.0609700054685427E-4	3.0313428727672646E-5	0.15881205310427698	3.3344771600439906E-4
36-37	6.062685745534529E-5	1.2125371491069058E-4	3.0313428727672646E-5	0.27661003714001287	3.3344771600439906E-4
38-39	9.094028618301794E-5	1.364104292745269E-4	3.0313428727672646E-5	0.4689335857027319	3.3344771600439906E-4
40-41	9.094028618301794E-5	1.5156714363836321E-4	3.0313428727672646E-5	0.6883876529767181	3.3344771600439906E-4
42-43	9.094028618301794E-5	1.5156714363836321E-4	3.0313428727672646E-5	0.920543046887599	3.3344771600439906E-4
44-45	9.094028618301794E-5	1.5156714363836321E-4	3.0313428727672646E-5	1.20101804619039	3.3344771600439906E-4
46-47	9.094028618301794E-5	1.6672385800219953E-4	3.0313428727672646E-5	1.5280544720188867	3.486044303682354E-4
48-49	9.094028618301794E-5	2.2735071545754485E-4	3.0313428727672646E-5	1.8802055735482597	3.940745734597444E-4
50-51	9.094028618301794E-5	2.728208585490538E-4	3.0313428727672646E-5	2.2573652537779623	4.24388002187417E-4
52-53	9.094028618301794E-5	2.728208585490538E-4	3.0313428727672646E-5	2.6452710444916256	4.3954471655125333E-4
54-55	9.094028618301794E-5	2.728208585490538E-4	3.0313428727672646E-5	3.0790258961558936	4.5470143091508964E-4
56-57	9.094028618301794E-5	2.728208585490538E-4	3.0313428727672646E-5	3.5831988427545447	4.5470143091508964E-4
58-59	9.094028618301794E-5	2.728208585490538E-4	3.0313428727672646E-5	4.124051038113681	4.8501485964276233E-4
60-61	9.094028618301794E-5	2.728208585490538E-4	3.0313428727672646E-5	4.694746804055209	5.15328288370435E-4
62-63	9.094028618301794E-5	2.728208585490538E-4	3.0313428727672646E-5	5.277507314630352	5.759551458257802E-4
64-65	9.094028618301794E-5	2.728208585490538E-4	3.0313428727672646E-5	5.895855790531782	5.759551458257802E-4
66-67	9.094028618301794E-5	3.486044303682354E-4	3.0313428727672646E-5	6.6026588514605615	5.911118601896165E-4
68-69	1.0609700054685427E-4	3.6376114473207175E-4	3.0313428727672646E-5	7.365632695821718	6.062685745534529E-4
70-71	1.2125371491069058E-4	3.7891785909590806E-4	3.0313428727672646E-5	8.168029154243213	6.214252889172892E-4
72-73	1.5156714363836321E-4	4.092312878235807E-4	3.0313428727672646E-5	8.989841363764782	6.820521463726345E-4
74-75	1.5156714363836321E-4	4.5470143091508964E-4	3.0313428727672646E-5	9.82665872050655	7.426790038279798E-4
76-77	1.5156714363836321E-4	4.5470143091508964E-4	3.0313428727672646E-5	10.72757382229298	8.033058612833251E-4
78-79	1.5156714363836321E-4	4.5470143091508964E-4	3.0313428727672646E-5	11.705575973333882	8.184625756471614E-4
80-81	1.5156714363836321E-4	4.5470143091508964E-4	3.0313428727672646E-5	12.712466821952258	8.48776004374834E-4
82-83	1.5156714363836321E-4	4.69858145278926E-4	3.0313428727672646E-5	13.733559511929547	9.094028618301793E-4
84-85	1.5156714363836321E-4	4.8501485964276233E-4	3.0313428727672646E-5	14.75363670204446	9.094028618301793E-4
86	1.5156714363836321E-4	4.8501485964276233E-4	3.0313428727672646E-5	15.552122728160084	9.094028618301793E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4670	0.0	41.589016	1
GGTATCA	2460	0.0	37.979042	1
TCAACGC	5260	0.0	35.49659	4
CAACGCA	5315	0.0	35.129265	5
ATCAACG	5485	0.0	34.20776	3
AACGCAG	5825	0.0	32.284943	6
TATCAAC	5940	0.0	31.743853	2
ACGCAGA	6875	0.0	27.148647	7
GTACATG	8500	0.0	25.827509	1
CGCAGAG	7270	0.0	25.29242	8
TACATGG	8535	0.0	24.833775	2
ACATGGG	8715	0.0	23.52979	3
GCAGAGT	8215	0.0	21.15431	9
CATGGGG	5885	0.0	20.65748	4
GTATAGC	1055	0.0	19.194786	1
GAGTACA	6190	0.0	18.21623	1
AGAGTAC	7315	0.0	17.364897	10-11
GTATTAG	1030	0.0	16.532845	1
CAGAGTA	7755	0.0	16.468351	10-11
GTATAGA	940	0.0	16.157314	1
>>END_MODULE