FastQCFastQC Report
Mon 15 Aug 2016
SRR2920863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298868
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47700.043.362881
GGTATCA26200.043.193951
TCAACGC55100.036.269254
CAACGCA55950.035.5437055
ATCAACG56600.035.3080523
TATCAAC59900.033.8368072
AACGCAG60150.033.1412626
ACGCAGA74600.026.4032887
CGCAGAG77700.025.162048
GTACATG87500.024.1823881
TACATGG88800.022.5038832
ACATGGG92250.020.8399563
GCAGAGT89550.020.4016279
GAGTACA66600.019.5624641
AGTACAT66550.017.6885382
AGAGTAC76900.017.58605610-11
CATGGGG66600.017.3909654
CAGAGTA83350.016.62367610-11
TAAGGTG13300.016.4155315
GTGTAGC19800.015.8498811
Produced by FastQC (version 0.11.5)