##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920863_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3298868
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.35996923793253	31.0	30.0	34.0	27.0	34.0
2	30.368876838964155	31.0	30.0	34.0	26.0	34.0
3	30.510163789518103	31.0	30.0	34.0	27.0	34.0
4	34.166061206450216	35.0	35.0	37.0	30.0	37.0
5	33.71801993896088	35.0	35.0	37.0	28.0	37.0
6	33.66808705289208	35.0	35.0	37.0	29.0	37.0
7	33.48978104004161	35.0	35.0	37.0	28.0	37.0
8	33.423267314727354	35.0	35.0	37.0	28.0	37.0
9	34.7644355578944	38.0	35.0	39.0	27.0	39.0
10-11	34.581972058293935	37.5	34.0	39.0	27.0	39.0
12-13	34.41590872990371	37.0	34.0	39.0	27.0	39.0
14-15	35.33689238247787	38.0	34.0	40.0	27.0	41.0
16-17	35.21767906445484	38.0	34.0	40.0	27.0	41.0
18-19	35.12857743929129	38.5	34.0	40.0	26.0	41.0
20-21	35.01680364294661	38.0	34.0	40.0	25.5	41.0
22-23	34.87960354885372	38.0	34.0	40.0	25.0	41.0
24-25	34.71947756018125	38.0	34.0	40.0	25.0	41.0
26-27	34.54467168737882	38.0	33.0	40.0	24.0	41.0
28-29	34.49209547032497	38.0	33.5	40.0	24.0	41.0
30-31	34.48698583877864	38.0	33.0	40.0	24.0	41.0
32-33	34.34937060227932	38.0	33.0	40.0	24.0	41.0
34-35	34.193849829699154	38.0	33.0	40.0	23.5	41.0
36-37	34.00797046138251	38.0	33.0	40.0	22.5	41.0
38-39	33.84078189851792	38.0	33.0	40.0	21.5	41.0
40-41	33.616582567110896	38.0	32.5	40.0	19.5	41.0
42-43	33.65900727158528	38.0	33.0	40.0	19.5	41.0
44-45	33.531634942653056	38.0	32.5	40.0	19.0	41.0
46-47	33.3130986144338	38.0	32.0	40.0	18.0	41.0
48-49	33.07428790724576	37.5	32.0	40.0	15.5	41.0
50-51	32.82538646590285	37.0	31.0	40.0	14.5	41.0
52-53	32.578419930715626	37.0	31.0	40.0	13.0	41.0
54-55	32.31617815565825	37.0	31.0	40.0	9.0	41.0
56-57	32.009658464661214	36.0	30.5	39.5	8.0	41.0
58-59	31.698592062489315	36.0	30.0	39.0	7.5	41.0
60-61	31.377493431079994	35.5	30.0	39.0	6.5	40.0
62-63	31.06600097366733	35.0	29.0	39.0	2.0	40.0
64-65	30.67417232214202	35.0	29.0	38.5	2.0	40.0
66-67	30.248335944329995	35.0	28.5	38.0	2.0	40.0
68-69	29.809113004824685	34.0	28.0	37.5	2.0	40.0
70-71	29.388447794819314	34.0	28.0	37.0	2.0	39.0
72-73	28.945476902986115	34.0	27.5	36.5	2.0	39.0
74-75	28.49529474959289	34.0	26.0	36.0	2.0	39.0
76-77	27.01417501397449	31.5	25.5	34.5	2.0	36.5
78-79	27.860657352764647	33.0	26.0	35.0	2.0	37.0
80-81	27.8820701525493	33.0	26.0	35.0	2.0	37.0
82-83	27.533349621749036	33.0	26.0	35.0	2.0	36.0
84-85	27.209245565448512	33.0	26.0	35.0	2.0	36.0
86-87	26.66051521309734	33.0	25.0	35.0	2.0	35.5
88-89	26.296727998816564	32.5	24.5	35.0	2.0	35.0
90-91	25.932321784321168	32.0	23.5	35.0	2.0	35.0
92-93	25.625093820061913	32.0	22.5	35.0	2.0	35.0
94-95	25.24454646260475	32.0	20.0	34.0	2.0	35.0
96-97	24.72624912545758	32.0	17.5	34.0	2.0	35.0
98-99	24.1396703353999	32.0	2.0	34.0	2.0	35.0
100-101	21.9509284700085	28.5	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66848.0
3	31042.0
4	16606.0
5	12016.0
6	10118.0
7	10254.0
8	11429.0
9	13534.0
10	15452.0
11	17337.0
12	19615.0
13	21589.0
14	23230.0
15	24729.0
16	26179.0
17	27475.0
18	26960.0
19	26173.0
20	26379.0
21	27914.0
22	30351.0
23	34467.0
24	40175.0
25	47959.0
26	56848.0
27	67115.0
28	79461.0
29	93831.0
30	110053.0
31	131490.0
32	158319.0
33	191980.0
34	240102.0
35	310938.0
36	412634.0
37	499309.0
38	314002.0
39	24954.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46178216089849	19.353663136374323	13.223199225955515	24.96135547677168
2	16.133018962868476	20.73102045913932	38.148237516626914	24.98772306136529
3	19.360140283094633	24.258497795798238	29.435074957412112	26.94628696369502
4	13.634776112887032	16.35520705495433	36.35336982726422	33.656647004894424
5	14.227248915922122	37.101352793380094	34.57026134280143	14.101136947896356
6	33.59949551382391	35.587943071721156	16.978035550737825	13.834525863717115
7	29.01134408718029	31.658257286653658	21.00773525343041	18.322663372735637
8	26.41708895952059	35.03899582118255	19.954369304509772	18.589545914787088
9	26.27861667333506	15.895500685154818	19.498801813884388	38.32708082762573
10-11	25.474782801871786	25.558681809863515	27.95610848206983	21.01042690619487
12-13	26.03436505170175	22.869832195980308	27.700083565894978	23.395719186422962
14-15	22.98424880571261	24.579929962656564	25.1126077081746	27.32321352345623
16-17	23.323134501873813	27.610223139188495	25.89607323502151	23.17056912391618
18-19	23.31848836463195	26.9117707065925	27.139989536367644	22.629751392407904
20-21	23.92671868598605	26.2305916901912	26.909399230536195	22.933290393286555
22-23	23.512599142359054	26.194319933932398	26.852401898290605	23.440679025417943
24-25	23.322737004331195	26.28658497468756	26.953879201242742	23.436798819738502
26-27	23.268117676358163	26.501660346520552	26.872587856401186	23.3576341207201
28-29	23.322962888383728	26.601070746672033	26.794989677065615	23.280976687878617
30-31	23.557917892453197	26.413330291133935	26.6675257595202	23.36122605689267
32-33	23.2631588682713	26.403445079900884	26.67574745561782	23.65764859620999
34-35	23.608249501282742	26.41671099624751	26.63202465942527	23.34301484304448
36-37	23.628928984061737	26.244125540714048	26.71159598061836	23.41534949460586
38-39	23.49804613409885	26.3842246137677	26.72687510935251	23.390854142780945
40-41	23.686574366545276	26.091847429030313	26.877555206560977	23.34402299786343
42-43	23.508701455308074	26.07982403816347	26.71980790933903	23.691666597189432
44-45	23.39938547148091	26.140445858375845	26.832249057035142	23.6279196131081
46-47	23.4390256623611	26.085756097797645	26.71667149824668	23.758546741594564
48-49	23.22441836106406	26.166544441874308	26.778764839398445	23.83027235766319
50-51	23.21670682799109	26.27990725098867	26.86177147216014	23.6416144488601
52-53	23.41852037057051	26.078704776675625	26.769791885664517	23.73298296708935
54-55	23.075618270429903	25.987905551552842	26.938820305442956	23.9976558725743
56-57	23.003745253634868	26.214516984450125	26.764474642121307	24.0172631197937
58-59	22.96830596255817	26.01132825271198	26.884918454557017	24.135447330172838
60-61	22.969890946666137	26.089979991576996	26.928596399575394	24.011532662181473
62-63	22.885605774091623	26.056399408523955	26.904671857366658	24.153322960017764
64-65	22.709369275554756	25.95456746783321	26.793492141807462	24.542571114804566
66-67	22.705912108220595	26.09449256357948	26.63922556783393	24.560369760366
68-69	22.876082827782348	25.86965667033747	26.66869751790636	24.585562983973823
70-71	22.66535158459456	25.61995047824589	26.76253162192765	24.952166315231906
72-73	22.71022601138609	25.68524599709586	26.609622982777715	24.994905008740336
74-75	22.633035042600067	25.724272443218492	26.484539868756933	25.158152645424508
76-77	22.528119452906566	25.49148653140646	26.758707778282165	25.22168623740481
78-79	22.48555508650111	25.51841429730545	26.729919746182723	25.26611087001072
80-81	22.450172224511274	25.51361894011814	26.633404187779746	25.402804647590845
82-83	22.394767661658207	25.458733049425387	26.654038654692286	25.492460634224116
84-85	22.273003780724927	25.29265715338592	26.64311311872592	25.791225947163227
86-87	22.117251271827723	25.231512153020475	26.756656962079173	25.894579613072626
88-89	22.168718833004917	25.137203786301416	26.59582563761425	26.09825174307942
90-91	21.97535528429202	25.079660001826703	26.652085445429613	26.29289926845166
92-93	22.05606538451886	24.945985676793196	26.624448924618743	26.3735000140692
94-95	21.806419793205407	24.84248198375848	26.725043947412853	26.626054275623257
96-97	21.77064122981819	24.628072942584144	26.809608481320772	26.79167734627689
98-99	21.668345580868444	24.59346075296801	26.727111619854615	27.011082046308932
100-101	21.774409786878326	24.414144652329213	26.743924315696855	27.06752124509561
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	28.0
2	47.5
3	85.5
4	74.0
5	78.5
6	127.0
7	244.0
8	386.0
9	407.0
10	349.5
11	335.5
12	351.5
13	378.0
14	447.0
15	494.5
16	529.0
17	608.5
18	695.0
19	785.0
20	948.0
21	1146.0
22	1427.0
23	1805.0
24	2258.0
25	2899.5
26	3871.0
27	5507.0
28	7788.5
29	11115.0
30	16028.0
31	22515.5
32	30688.5
33	37840.5
34	44266.0
35	52851.0
36	63446.5
37	73100.0
38	81713.0
39	89502.5
40	96011.0
41	103151.5
42	110355.5
43	118577.5
44	126619.5
45	134179.0
46	140856.5
47	143937.5
48	146145.0
49	147370.5
50	146310.0
51	144432.0
52	141226.0
53	138276.5
54	133466.5
55	124215.5
56	112355.5
57	99927.5
58	88335.0
59	75537.5
60	61906.5
61	50212.0
62	40502.5
63	30999.5
64	22418.5
65	16723.5
66	12808.5
67	9634.0
68	7170.5
69	5246.5
70	3747.0
71	2651.5
72	1925.0
73	1466.5
74	1099.0
75	766.0
76	487.0
77	283.0
78	161.5
79	85.5
80	44.0
81	23.0
82	14.0
83	6.5
84	4.5
85	2.0
86	0.5
87	2.5
88	2.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18291122894277673
2	0.0
3	0.011973804347430695
4	0.012731640065622512
5	0.006426446890266601
6	0.11015899999636239
7	0.14335220445316393
8	0.12598260979220752
9	0.14314001045207023
10-11	0.11625199917062459
12-13	0.11711593188936326
14-15	0.11857097646829155
16-17	0.06815974449417193
18-19	0.08152796656307557
20-21	0.03780084562340778
22-23	0.08639327187386703
24-25	0.05976292473660661
26-27	0.06872054292563388
28-29	0.04110500935472411
30-31	0.024720601127417044
32-33	0.032238331451879855
34-35	0.01990076595971709
36-37	0.024205272839046606
38-39	0.03173815987787326
40-41	0.02493279512851075
42-43	0.012898363923624711
44-45	0.027054735139447835
46-47	0.03395104017499336
48-49	0.009124342047029466
50-51	0.015277968078747012
52-53	0.006805364749362509
54-55	0.012504289350164966
56-57	0.004834991882063787
58-59	0.0037740218765952445
60-61	0.022947265546848193
62-63	0.010321722481772535
64-65	0.007290379609005271
66-67	0.041862845072915926
68-69	0.09219829347521635
70-71	0.021871138827015812
72-73	0.04583390423624104
74-75	0.0885909954566233
76-77	0.23432280406490955
78-79	0.07998198169796426
80-81	0.01688457980131366
82-83	0.011549416345243277
84-85	0.012928677352352383
86-87	0.2524502344440578
88-89	0.614134909308284
90-91	0.26650050865933406
92-93	0.3499836913753445
94-95	0.0011822237203792333
96-97	0.004137783021327316
98-99	0.0033041637313163184
100-101	0.005016872454429823
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3298868.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.38277843129035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94062498167922	40.39654472000183
2	13.641811798737097	15.110428805016381
3	5.4309903980981655	9.023500136410084
4	2.749087527090432	6.090084216042933
5	1.556892378268208	4.311251281349643
6	0.9486710489688571	3.152402310553321
7	0.6016520445085011	2.3324813311623007
8	0.44425173152760344	1.968311617192828
9	0.31341235109674126	1.562188211857854
>10	1.304630146857753	11.944212612311683
>50	0.04877923400474175	1.8410619878854835
>100	0.017931687252016906	1.661373212137716
>500	0.0010447289704391056	0.42935160091349844
>1k	2.1994294031261005E-4	0.17680795716442999
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.0313428727672646E-5	0.0
8	0.0	0.0	0.0	3.0313428727672646E-5	0.0
9	0.0	0.0	0.0	3.0313428727672646E-5	0.0
10-11	0.0	0.0	0.0	6.062685745534529E-5	0.0
12-13	0.0	0.0	0.0	1.2125371491069058E-4	1.6672385800219953E-4
14-15	0.0	0.0	0.0	1.2125371491069058E-4	2.2735071545754485E-4
16-17	0.0	0.0	0.0	1.2125371491069058E-4	3.1829100164056275E-4
18-19	0.0	0.0	0.0	2.2735071545754485E-4	3.6376114473207175E-4
20-21	0.0	0.0	0.0	3.1829100164056275E-4	3.940745734597444E-4
22-23	0.0	0.0	0.0	4.092312878235807E-4	3.940745734597444E-4
24-25	0.0	1.5156714363836323E-5	0.0	8.336192900109977E-4	3.940745734597444E-4
26-27	0.0	3.0313428727672646E-5	0.0	0.0017278654374773407	3.940745734597444E-4
28-29	0.0	3.0313428727672646E-5	0.0	0.00762382732500967	3.940745734597444E-4
30-31	0.0	4.547014309150897E-5	0.0	0.03305679402752702	3.940745734597444E-4
32-33	0.0	6.062685745534529E-5	0.0	0.0865448390175054	4.5470143091508964E-4
34-35	0.0	7.578357181918161E-5	0.0	0.15967598582301568	4.5470143091508964E-4
36-37	0.0	1.5156714363836321E-4	0.0	0.2798687307282377	4.5470143091508964E-4
38-39	0.0	2.121940010937085E-4	0.0	0.4743142193018939	5.456417170981076E-4
40-41	0.0	2.121940010937085E-4	0.0	0.6969511965922857	5.456417170981076E-4
42-43	0.0	3.940745734597444E-4	0.0	0.9327138885217596	5.456417170981076E-4
44-45	0.0	4.5470143091508964E-4	0.0	1.215947409838769	5.456417170981076E-4
46-47	0.0	4.5470143091508964E-4	0.0	1.5477582006918738	5.456417170981076E-4
48-49	0.0	4.5470143091508964E-4	0.0	1.90201608551782	5.456417170981076E-4
50-51	0.0	4.5470143091508964E-4	0.0	2.2769174153073113	5.456417170981076E-4
52-53	0.0	4.5470143091508964E-4	0.0	2.662443601865852	6.972088607364708E-4
54-55	0.0	4.5470143091508964E-4	0.0	3.0946069985219173	7.275222894641435E-4
56-57	0.0	4.5470143091508964E-4	0.0	3.5971733333980023	7.275222894641435E-4
58-59	0.0	4.5470143091508964E-4	0.0	4.137070655752216	7.275222894641435E-4
60-61	0.0	4.8501485964276233E-4	0.0	4.708312063410842	7.275222894641435E-4
62-63	0.0	4.8501485964276233E-4	0.0	5.29101194712853	7.578357181918161E-4
64-65	0.0	4.8501485964276233E-4	0.0	5.907647714306847	7.578357181918161E-4
66-67	0.0	6.062685745534529E-4	0.0	6.613071514228517	7.729924325556524E-4
68-69	1.5156714363836323E-5	6.062685745534529E-4	0.0	7.372347120284898	7.881491469194888E-4
70-71	3.0313428727672646E-5	6.062685745534529E-4	0.0	8.17209115369272	8.184625756471614E-4
72-73	3.0313428727672646E-5	6.365820032811255E-4	0.0	8.99375179607065	9.245595761940156E-4
74-75	3.0313428727672646E-5	6.365820032811255E-4	0.0	9.836540898271771	0.0010003431480131973
76-77	3.0313428727672646E-5	6.972088607364709E-4	0.0	10.74104814136243	0.0010003431480131973
78-79	3.0313428727672646E-5	6.972088607364709E-4	0.0	11.718368240256961	0.0010003431480131973
80-81	3.0313428727672646E-5	6.972088607364709E-4	0.0	12.728063081032644	0.0010154998623770335
82-83	3.0313428727672646E-5	6.972088607364709E-4	0.0	13.750989733447959	0.0010609700054685426
84-85	3.0313428727672646E-5	7.123655751003072E-4	0.0	14.775735191586932	0.0010609700054685426
86-87	3.0313428727672646E-5	7.426790038279798E-4	0.0	15.850224986268017	0.0010912834341962152
88-89	3.0313428727672646E-5	7.578357181918161E-4	0.0	16.981143228525664	0.0011215968629238878
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4770	0.0	43.36288	1
GGTATCA	2620	0.0	43.19395	1
TCAACGC	5510	0.0	36.26925	4
CAACGCA	5595	0.0	35.543705	5
ATCAACG	5660	0.0	35.308052	3
TATCAAC	5990	0.0	33.836807	2
AACGCAG	6015	0.0	33.141262	6
ACGCAGA	7460	0.0	26.403288	7
CGCAGAG	7770	0.0	25.16204	8
GTACATG	8750	0.0	24.182388	1
TACATGG	8880	0.0	22.503883	2
ACATGGG	9225	0.0	20.839956	3
GCAGAGT	8955	0.0	20.401627	9
GAGTACA	6660	0.0	19.562464	1
AGTACAT	6655	0.0	17.688538	2
AGAGTAC	7690	0.0	17.586056	10-11
CATGGGG	6660	0.0	17.390965	4
CAGAGTA	8335	0.0	16.623676	10-11
TAAGGTG	1330	0.0	16.415531	5
GTGTAGC	1980	0.0	15.849881	1
>>END_MODULE