FastQCFastQC Report
Mon 15 Aug 2016
SRR2920862_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920862_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3416320
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.045.7154961
GTATCAA45650.043.449581
TCAACGC54950.035.1428344
ATCAACG55800.034.6897053
CAACGCA56400.033.9953545
TATCAAC59700.032.500852
AACGCAG60650.031.8344526
GTACATG82200.026.705191
ACGCAGA71250.026.2581527
CGCAGAG72900.025.2220448
TACATGG87200.024.6709062
ACATGGG89200.023.2432023
GCAGAGT86100.020.3963629
CATGGGG59550.019.9498844
CGCCGTA5700.019.37382392
GAGTACA60650.018.741971
AGAGTAC70250.017.81872410-11
CAGAGTA78250.016.46577810-11
AGTACAT65000.015.9486472
GTATAGC10700.015.913551
Produced by FastQC (version 0.11.5)