##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920862_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3416320
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.123251627482205	31.0	28.0	31.0	16.0	34.0
2	28.157543204383664	31.0	28.0	33.0	16.0	34.0
3	28.222547653615585	31.0	28.0	33.0	16.0	34.0
4	31.657843234825776	35.0	32.0	37.0	19.0	37.0
5	31.609603023136007	35.0	32.0	37.0	19.0	37.0
6	31.725582498126638	35.0	32.0	37.0	19.0	37.0
7	31.54710595026227	35.0	32.0	37.0	18.0	37.0
8	31.48102490399026	35.0	32.0	37.0	17.0	37.0
9	32.69549925065568	37.0	32.0	39.0	17.0	39.0
10-11	32.48406765174222	37.0	32.0	39.0	17.0	39.0
12-13	32.30549524049269	37.0	32.0	39.0	16.0	39.0
14-15	33.0077052208224	38.0	32.0	40.0	14.5	41.0
16-17	32.8860316071094	38.0	32.0	40.0	11.0	41.0
18-19	32.767380104908206	38.0	31.5	40.0	10.0	41.0
20-21	32.677766427032594	38.0	31.0	40.0	10.0	41.0
22-23	32.449062587813785	37.0	31.0	40.0	10.0	41.0
24-25	32.254093147011986	37.0	31.0	40.0	10.0	41.0
26-27	32.280513974100785	37.0	31.0	40.0	9.0	41.0
28-29	32.11130763511615	37.0	30.5	40.0	8.0	41.0
30-31	31.858981886942672	37.0	30.0	40.0	7.5	41.0
32-33	31.608515010303485	37.0	30.0	40.0	2.0	41.0
34-35	31.272191422349195	37.0	30.0	40.0	2.0	41.0
36-37	30.967451673145373	36.0	29.5	40.0	2.0	41.0
38-39	30.766685205133008	36.0	29.0	39.5	2.0	41.0
40-41	30.5664770864556	36.0	28.5	39.0	2.0	40.5
42-43	30.32968852449419	36.0	28.0	39.0	2.0	40.0
44-45	30.056246487448483	35.0	27.0	39.0	2.0	40.0
46-47	29.808239714078304	35.0	27.0	39.0	2.0	40.0
48-49	29.51070332989884	35.0	26.0	39.0	2.0	40.0
50-51	28.41921760842076	34.0	25.0	38.0	2.0	39.5
52-53	28.890012206116523	34.5	26.0	38.5	2.0	39.5
54-55	29.53213501662608	35.0	27.0	39.0	2.0	40.0
56-57	29.491271163122892	35.0	27.0	39.0	2.0	40.0
58-59	29.26263757493443	35.0	26.0	39.0	2.0	40.0
60-61	28.94232229416448	35.0	26.0	39.0	2.0	40.0
62-63	28.55921166635444	34.0	26.0	38.0	2.0	40.0
64-65	28.14473395349382	34.0	25.0	38.0	2.0	40.0
66-67	27.761620837626452	34.0	23.5	37.5	2.0	40.0
68-69	27.32395296693518	33.5	22.5	37.0	2.0	39.5
70-71	26.989974299831395	33.0	22.0	36.5	2.0	39.0
72-73	26.522902714031474	33.0	21.0	36.0	2.0	39.0
74-75	26.077663831257027	32.0	20.0	35.5	2.0	38.5
76-77	25.65456602426002	32.0	18.5	35.0	2.0	37.5
78-79	25.182491101536158	32.0	16.0	35.0	2.0	37.0
80-81	24.724076491663546	31.5	9.5	35.0	2.0	36.5
82-83	24.24402090553578	31.0	7.0	35.0	2.0	36.0
84-85	23.828678812289247	31.0	2.0	34.0	2.0	35.5
86-87	23.364120310743722	31.0	2.0	34.0	2.0	35.0
88-89	22.82354960893593	30.5	2.0	34.0	2.0	35.0
90-91	22.31701318963095	30.0	2.0	34.0	2.0	35.0
92-93	21.7730446796553	30.0	2.0	34.0	2.0	35.0
94-95	21.132472367928063	29.0	2.0	34.0	2.0	35.0
96-97	20.487905846056577	29.0	2.0	34.0	2.0	35.0
98	20.085088340670662	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	200642.0
3	33948.0
4	24803.0
5	19716.0
6	18352.0
7	18796.0
8	21575.0
9	24580.0
10	26713.0
11	28587.0
12	28913.0
13	28548.0
14	27251.0
15	29077.0
16	30734.0
17	32948.0
18	33991.0
19	34221.0
20	35264.0
21	37496.0
22	40619.0
23	44952.0
24	51591.0
25	58294.0
26	67348.0
27	77697.0
28	88806.0
29	101741.0
30	117190.0
31	137210.0
32	161559.0
33	192288.0
34	236730.0
35	292976.0
36	363255.0
37	390938.0
38	233291.0
39	23680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.008405709136966	18.336297110618275	12.796467087383004	24.85883009286176
2	15.765596691818434	19.676319823676078	39.26383942530769	25.29424405919781
3	19.010610700052084	23.72794121737165	29.47525004369525	27.786198038881015
4	13.087647967754346	15.688783053231212	37.063181506526476	34.16038747248797
5	14.048274373968153	36.9670520649221	34.816625243990735	14.168048317119009
6	33.32594548588369	35.53372122948225	17.25564987341602	13.884683411218033
7	28.881193318847785	31.673057522385562	21.295367610335212	18.150381548431444
8	26.386693364246323	34.65158487605532	20.28006112340605	18.681660636292317
9	26.573740164855753	15.62037514050206	19.824811493068566	37.98107320157362
10-11	25.268999362910687	25.450832526299877	28.18051068716222	21.099657423627217
12-13	25.794867178792515	22.831518791974304	27.90864869176654	23.46496533746664
14-15	23.032946130700864	24.51847996597402	25.249362378750973	27.19921152457414
16-17	23.213224052065797	27.42878729466219	26.154058719507606	23.203929933764407
18-19	23.180071243153495	26.765654533661877	27.429990351574567	22.624283871610057
20-21	23.867382113487714	26.119369865842735	27.154019042290408	22.859228978379143
22-23	23.53329730554848	26.031007236369565	26.918922688172785	23.51677276990917
24-25	23.30347711386852	26.119908446980133	27.055326194611233	23.521288244540113
26-27	23.22281947786015	26.346809347964495	26.944300952398248	23.48607022177711
28-29	23.312390757306666	26.557718801692587	26.796397978453708	23.33349246254704
30-31	23.48051000263863	26.321504787168458	26.716113427763734	23.481871782429174
32-33	23.192993966509494	26.351701979500742	26.689189238643092	23.766114815346675
34-35	23.675936529117642	26.27677614528052	26.672562470881328	23.3747248547205
36-37	23.69355639117429	26.020473149252076	26.682163168040262	23.603807291533375
38-39	23.4530898655468	26.20173934264874	26.701195752128605	23.643975039675862
40-41	23.682236455377726	26.024188784141465	26.828589803118035	23.464984957362773
42-43	23.498863499592414	25.99153727129096	26.698670220309285	23.810929008807346
44-45	23.46344510054465	26.03982234900525	26.830991926838045	23.665740623612056
46-47	23.57468009858852	25.972985711246587	26.604810832983667	23.847523357181224
48-49	23.356229595690017	26.086930972888666	26.650212227594245	23.906627203827075
50-51	23.281780517833514	26.188796976432588	26.764500523921075	23.76492198181282
52-53	23.40257589258285	26.042070579962413	26.804604986393237	23.7507485410615
54-55	23.078184908930158	25.93656456173075	26.99879984625097	23.98645068308812
56-57	23.02951184288407	26.05870922350156	26.930137064403638	23.981641869210733
58-59	23.01998592478428	25.813504860944803	27.013997346572065	24.152511867698852
60-61	22.958895477979446	25.80587046235901	27.13463610410175	24.10059795555979
62-63	22.961859228545798	25.72569368026725	27.182590462500528	24.129856628686426
64-65	22.834078318220502	25.684413524672696	27.065852156389504	24.415656000717302
66-67	22.794817503202818	25.688793790130816	26.99147492626236	24.524913780404002
68-69	22.92354293413697	25.494396988413413	27.0567897715376	24.525270305912017
70-71	22.70476036049215	25.33924469724308	27.181713781476642	24.774281160788128
72-73	22.677175969485972	25.265751655160855	27.188430094079425	24.86864228127374
74-75	22.60367933170441	25.431607812424062	27.154955651004258	24.809757204867267
76-77	22.585128081945193	25.168291635808792	27.309113354738567	24.937466927507444
78-79	22.49014917808386	25.136410960565435	27.354928964110663	25.018510897240038
80-81	22.52772862439884	25.04000881089614	27.401887000256984	25.030375564448033
82-83	22.495428799999058	25.00255505557393	27.45806625170998	25.04394989271703
84-85	22.42062754943887	24.963697179929095	27.471833284704417	25.14384198592762
86-87	22.41421204993269	24.858387354942565	27.53558246656259	25.191818128562154
88-89	22.379423186605308	24.63801728786279	27.56917502591387	25.41338449961803
90-91	22.33863580092265	24.619012108611248	27.645474712366514	25.396877378099585
92-93	22.467686266966318	24.394006917580064	27.716031209137494	25.42227560631613
94-95	22.292549624163165	24.23576710818641	27.85067910845896	25.621004159191465
96-97	22.482155451943182	23.939279709793983	27.850564401244505	25.728000437018334
98	22.462675762453475	23.857645004115895	27.778477709713066	25.90120152371756
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	29.5
2	59.0
3	103.5
4	119.0
5	152.5
6	243.0
7	432.5
8	670.5
9	814.0
10	867.5
11	965.5
12	1124.5
13	1275.5
14	1443.0
15	1616.5
16	1662.0
17	1714.5
18	1818.0
19	1882.5
20	1951.0
21	2044.5
22	2314.0
23	2738.0
24	3344.5
25	4347.0
26	6047.0
27	8585.5
28	12108.5
29	16973.5
30	23628.0
31	32341.5
32	40361.0
33	47327.5
34	55704.5
35	65284.0
36	75459.5
37	85278.0
38	93799.0
39	101272.0
40	108125.0
41	115233.0
42	122751.5
43	129719.0
44	136426.0
45	142283.0
46	146236.5
47	148578.0
48	149662.5
49	149396.0
50	148899.0
51	147143.5
52	143553.5
53	140435.5
54	135316.0
55	126861.0
56	114903.5
57	102463.0
58	92021.0
59	79321.0
60	65619.5
61	53582.5
62	43172.5
63	33056.0
64	24055.0
65	17816.0
66	13326.0
67	10056.5
68	7514.0
69	5493.5
70	3955.0
71	2930.5
72	2170.0
73	1581.0
74	1217.5
75	900.0
76	607.5
77	371.0
78	207.0
79	115.0
80	67.5
81	41.5
82	21.5
83	16.5
84	13.5
85	9.0
86	6.0
87	9.0
88	7.5
89	2.5
90	5.0
91	8.0
92	7.5
93	4.5
94	3.5
95	3.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3642223210940427
2	0.023065754964406143
3	0.01806036905207943
4	0.01586502435369052
5	0.021046037841888347
6	0.023446281378793555
7	0.014694173847883102
8	0.00819595354065193
9	0.0
10-11	4.390689396777819E-5
12-13	0.004902936493068564
14-15	0.003132025103034844
16-17	0.005298098538778569
18-19	0.036735434619707755
20-21	0.07617846103409516
22-23	0.09406320251030348
24-25	0.062157526227051334
26-27	0.07213902678905956
28-29	0.05573248407643312
30-31	0.04891227988010491
32-33	0.01860188741101536
34-35	0.3241353269014612
36-37	0.49301587673285874
38-39	0.4485089218808543
40-41	0.458183074185088
42-43	0.40949032877482205
44-45	0.3843463141626077
46-47	0.3829852004496066
48-49	0.3741013722367928
50-51	0.3705302781940802
52-53	0.4303753746721619
54-55	0.42948260116148373
56-57	0.4006065005620082
58-59	0.3856488853503185
60-61	0.40177735106781565
62-63	0.38225341888347697
64-65	0.4298484919445485
66-67	0.45109942862495317
68-69	0.39406437336080935
70-71	0.35211865399025855
72-73	0.33926856968902214
74-75	0.3219985247283627
76-77	0.31970073061071563
78-79	0.31886649962532787
80-81	0.3350096009741476
82-83	0.3309408954664668
84-85	0.3302237495316598
86-87	0.3165833411390034
88-89	0.35873395934807045
90-91	0.3381708973398277
92-93	0.3286431013488198
94-95	0.33519986418134134
96-97	0.334526625140502
98	0.3286284657174972
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3416320.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.56099170534197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.57874034758602	47.75824191048621
2	12.035159854201858	14.817927519139703
3	4.45347525799645	8.224810602523954
4	2.146909639352674	5.286635460012346
5	1.1643482987475677	3.583921798066403
6	0.6900628535803782	2.548857216325576
7	0.445111204246614	1.9181041006806405
8	0.2936177200068819	1.4460318420708063
9	0.21178280123812457	1.1733803343318845
>10	0.9282537815524319	9.614742523209793
>50	0.03903251346745478	1.6402315900552036
>100	0.012215543896852404	1.2942053982127604
>500	8.123381596268447E-4	0.33490881134976885
>1k	4.778459670401158E-4	0.358000893534928
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.927126264518546E-5
2	0.0	0.0	0.0	0.0	2.927126264518546E-5
3	0.0	0.0	0.0	2.927126264518546E-5	2.927126264518546E-5
4	0.0	0.0	0.0	1.1708505058074185E-4	2.927126264518546E-5
5	0.0	0.0	0.0	1.463563132259273E-4	2.927126264518546E-5
6	0.0	0.0	0.0	1.463563132259273E-4	2.927126264518546E-5
7	0.0	0.0	0.0	1.463563132259273E-4	2.927126264518546E-5
8	0.0	0.0	0.0	1.463563132259273E-4	2.927126264518546E-5
9	0.0	0.0	0.0	1.463563132259273E-4	2.927126264518546E-5
10-11	0.0	0.0	0.0	1.463563132259273E-4	2.927126264518546E-5
12-13	0.0	0.0	0.0	2.1953446983889095E-4	1.6099194454852005E-4
14-15	0.0	1.463563132259273E-5	0.0	2.341701011614837E-4	1.902632071937055E-4
16-17	0.0	5.854252529037092E-5	0.0	2.6344136380666914E-4	2.1953446983889095E-4
18-19	0.0	5.854252529037092E-5	0.0	2.927126264518546E-4	2.6344136380666914E-4
20-21	0.0	1.7562757587111276E-4	0.0	2.927126264518546E-4	3.0734825777444733E-4
22-23	0.0	1.7562757587111276E-4	0.0	4.683402023229674E-4	3.219838890970401E-4
24-25	0.0	2.0489883851629824E-4	0.0	7.756884600974148E-4	3.219838890970401E-4
26-27	0.0	2.341701011614837E-4	0.0	0.0015221056575496442	3.219838890970401E-4
28-29	0.0	2.341701011614837E-4	0.0	0.0069519248782315465	3.219838890970401E-4
30-31	0.0	2.780769951292619E-4	0.0	0.03247646590483327	3.366195204196328E-4
32-33	0.0	3.0734825777444733E-4	0.0	0.08703809947545897	3.512551517422255E-4
34-35	0.0	3.219838890970401E-4	0.0	0.1584892515923567	3.512551517422255E-4
36-37	0.0	3.219838890970401E-4	0.0	0.28034551798426377	3.512551517422255E-4
38-39	0.0	3.80526414387411E-4	0.0	0.4814683636193331	3.80526414387411E-4
40-41	0.0	3.951620457100037E-4	0.0	0.7137211970775572	3.80526414387411E-4
42-43	0.0	4.8297583364556015E-4	0.0	0.9617805123641814	3.951620457100037E-4
44-45	0.0	5.561539902585238E-4	0.0	1.263230610715624	4.097976770325965E-4
46-47	0.0	5.561539902585238E-4	0.0	1.6134759038965905	4.097976770325965E-4
48-49	0.0	5.561539902585238E-4	0.0	1.9895823576245786	4.097976770325965E-4
50-51	0.0	5.561539902585238E-4	0.0	2.392867178718621	4.3906893967778196E-4
52-53	0.0	6.146965155488947E-4	0.0	2.8103631978269012	4.976114649681529E-4
54-55	0.0	6.732390408392656E-4	0.0	3.281323178156613	4.976114649681529E-4
56-57	0.0	6.732390408392656E-4	0.0	3.8092450355938556	4.976114649681529E-4
58-59	0.0	7.317815661296366E-4	0.0	4.367667548707381	4.976114649681529E-4
60-61	0.0	7.317815661296366E-4	0.0	4.963396286062196	4.976114649681529E-4
62-63	0.0	7.317815661296366E-4	0.0	5.569252880292244	5.561539902585238E-4
64-65	0.0	7.61052828774822E-4	0.0	6.222836268265268	5.707896215811166E-4
66-67	2.927126264518546E-5	7.61052828774822E-4	0.0	6.96096969838891	6.000608842263019E-4
68-69	2.927126264518546E-5	7.61052828774822E-4	0.0	7.757953002060697	6.146965155488947E-4
70-71	2.927126264518546E-5	7.61052828774822E-4	0.0	8.591788825402773	6.293321468714874E-4
72-73	2.927126264518546E-5	7.61052828774822E-4	0.0	9.437991757212439	6.732390408392656E-4
74-75	2.927126264518546E-5	7.61052828774822E-4	0.0	10.31080226676658	6.732390408392656E-4
76-77	2.927126264518546E-5	7.61052828774822E-4	0.0	11.250922044773322	6.878746721618583E-4
78-79	2.927126264518546E-5	7.61052828774822E-4	0.0	12.260736699138253	7.171459348070438E-4
80-81	2.927126264518546E-5	7.61052828774822E-4	0.0	13.307286788122891	7.317815661296366E-4
82-83	2.927126264518546E-5	7.61052828774822E-4	0.0	14.365077041963282	7.317815661296366E-4
84-85	2.927126264518546E-5	8.19595354065193E-4	0.0	15.421462275196703	7.61052828774822E-4
86	2.927126264518546E-5	8.488666167103784E-4	0.0	16.2460483795429	7.61052828774822E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2265	0.0	45.715496	1
GTATCAA	4565	0.0	43.44958	1
TCAACGC	5495	0.0	35.142834	4
ATCAACG	5580	0.0	34.689705	3
CAACGCA	5640	0.0	33.995354	5
TATCAAC	5970	0.0	32.50085	2
AACGCAG	6065	0.0	31.834452	6
GTACATG	8220	0.0	26.70519	1
ACGCAGA	7125	0.0	26.258152	7
CGCAGAG	7290	0.0	25.222044	8
TACATGG	8720	0.0	24.670906	2
ACATGGG	8920	0.0	23.243202	3
GCAGAGT	8610	0.0	20.396362	9
CATGGGG	5955	0.0	19.949884	4
CGCCGTA	570	0.0	19.373823	92
GAGTACA	6065	0.0	18.74197	1
AGAGTAC	7025	0.0	17.818724	10-11
CAGAGTA	7825	0.0	16.465778	10-11
AGTACAT	6500	0.0	15.948647	2
GTATAGC	1070	0.0	15.91355	1
>>END_MODULE