FastQCFastQC Report
Mon 15 Aug 2016
SRR2920862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3416320
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25950.044.3368761
GTATCAA48050.043.8326151
TCAACGC58000.035.435084
CAACGCA59700.034.3440175
ATCAACG60300.034.004783
AACGCAG64650.031.8612586
TATCAAC65350.031.4478072
ACGCAGA81100.025.3986477
CGCAGAG81950.024.7866238
GTACATG92100.023.023021
TACATGG92250.022.6892592
ACATGGG96450.020.7182663
GCAGAGT96300.020.008869
TAAGGCG11200.019.0693655
GGACCGT4101.8189894E-1218.5215726
GTAAGGT13600.018.1483864
CATGGGG67150.018.0246874
TAAGGTG17050.017.815475
GAGTACA70400.017.7611121
GTATAGG8300.017.7570441
Produced by FastQC (version 0.11.5)