##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920862_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3416320
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.36200180310978	31.0	30.0	34.0	27.0	34.0
2	30.386305147058824	31.0	30.0	34.0	26.0	34.0
3	30.522169176189585	31.0	30.0	34.0	27.0	34.0
4	34.17775969464219	35.0	35.0	37.0	30.0	37.0
5	33.73818699653428	35.0	35.0	37.0	28.0	37.0
6	33.6853222180592	35.0	35.0	37.0	29.0	37.0
7	33.50359509647808	35.0	35.0	37.0	28.0	37.0
8	33.43657297911203	35.0	35.0	37.0	28.0	37.0
9	34.783989204758335	38.0	35.0	39.0	27.0	39.0
10-11	34.60210972625515	38.0	34.0	39.0	27.0	39.0
12-13	34.43658307769764	37.0	34.0	39.0	27.0	39.0
14-15	35.35728883711128	38.0	34.0	40.0	27.0	41.0
16-17	35.238183044913825	38.0	34.0	40.0	27.0	41.0
18-19	35.15577375655676	38.5	34.0	40.0	26.0	41.0
20-21	35.05084359778944	38.5	34.0	40.0	25.5	41.0
22-23	34.916382540277255	38.0	34.0	40.0	25.0	41.0
24-25	34.75322422958037	38.0	34.0	40.0	25.0	41.0
26-27	34.58512273440427	38.0	33.0	40.0	24.0	41.0
28-29	34.53410936329149	38.0	33.5	40.0	24.0	41.0
30-31	34.517744532128134	38.0	33.0	40.0	24.0	41.0
32-33	34.3793135596197	38.0	33.0	40.0	24.0	41.0
34-35	34.22745571257961	38.0	33.0	40.0	23.5	41.0
36-37	34.04636407011053	38.0	33.0	40.0	22.5	41.0
38-39	33.870814063085426	38.0	33.0	40.0	21.5	41.0
40-41	33.64834544187898	38.0	32.5	40.0	19.5	41.0
42-43	33.660259284844514	38.0	33.0	40.0	19.5	41.0
44-45	33.52709421248595	38.0	32.5	40.0	18.5	41.0
46-47	33.29801218855377	38.0	32.0	40.0	17.5	41.0
48-49	33.057522860856125	37.5	32.0	40.0	15.0	41.0
50-51	32.817204623688646	37.0	31.0	40.0	14.5	41.0
52-53	32.57004013090109	37.0	31.0	40.0	12.5	41.0
54-55	32.30609193518171	37.0	31.0	40.0	9.0	41.0
56-57	31.999681821375045	36.0	30.5	39.5	8.0	41.0
58-59	31.690261597274258	36.0	30.0	39.0	7.5	41.0
60-61	31.377819554374298	35.5	30.0	39.0	6.5	40.0
62-63	31.070232443096664	35.0	29.5	39.0	2.0	40.0
64-65	30.67668134132634	35.0	29.0	38.5	2.0	40.0
66-67	30.260172349194455	35.0	28.5	38.0	2.0	40.0
68-69	29.828995088282127	34.0	28.0	37.5	2.0	40.0
70-71	29.404401373407644	34.0	28.0	37.0	2.0	39.0
72-73	28.964374970728738	34.0	27.5	36.0	2.0	39.0
74-75	28.52007920803672	34.0	26.0	36.0	2.0	39.0
76-77	27.035262943752343	31.5	25.5	34.5	2.0	36.5
78-79	27.87533676587673	33.0	26.0	35.0	2.0	37.0
80-81	27.89665444103597	33.0	26.0	35.0	2.0	37.0
82-83	27.545908901976397	33.0	26.0	35.0	2.0	36.0
84-85	27.215602900196703	33.0	26.0	35.0	2.0	36.0
86-87	26.666554801657924	33.0	25.0	35.0	2.0	35.5
88-89	26.29475707779131	32.5	24.5	35.0	2.0	35.0
90-91	25.929933524962532	32.0	23.5	35.0	2.0	35.0
92-93	25.619156431481827	32.0	22.5	35.0	2.0	35.0
94-95	25.240880684479208	32.0	20.0	34.0	2.0	35.0
96-97	24.709815093433868	32.0	17.0	34.0	2.0	35.0
98-99	24.1104362296272	32.0	2.0	34.0	2.0	35.0
100-101	21.948409692300487	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68320.0
3	31922.0
4	17548.0
5	12199.0
6	10491.0
7	10566.0
8	12010.0
9	14327.0
10	16453.0
11	18181.0
12	20374.0
13	22561.0
14	24585.0
15	26290.0
16	27565.0
17	28734.0
18	27932.0
19	26895.0
20	27313.0
21	28537.0
22	31150.0
23	34941.0
24	41120.0
25	48921.0
26	57800.0
27	68916.0
28	82352.0
29	96800.0
30	114951.0
31	134741.0
32	162779.0
33	198675.0
34	246795.0
35	319949.0
36	428458.0
37	520096.0
38	327484.0
39	27589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.9469134509952	19.71459330234746	13.508034303232813	24.830458943424535
2	15.923801049082053	20.851179046459347	38.28924105470213	24.935778849756463
3	18.86132216820644	24.543469694725275	29.8346191926283	26.760588944439988
4	13.475403616298989	16.607111281817684	36.73040058924993	33.18708451263339
5	14.02826143466358	37.28059801458035	34.7890755082398	13.90206504251627
6	33.242045874438816	36.099814133420146	17.05374952053572	13.604390471605313
7	28.71269918366976	31.883721087021584	21.39547944928414	18.008100280024518
8	26.33974285705912	35.08793678984558	20.166729677530913	18.405590675564387
9	26.407486269856534	15.67209110905791	19.809249496991328	38.111173124094236
10-11	25.311659731940615	25.636151283453025	28.235556309013365	20.81663267559299
12-13	25.890718661581268	22.9005959175181	28.018541284175146	23.190144136725486
14-15	23.008440707346075	24.65927631612687	25.289270608887033	27.043012367640024
16-17	23.240527500144623	27.57211240975651	26.14448770550325	23.042872384595615
18-19	23.16649358220389	26.858216727136373	27.460272355359734	22.5150173353
20-21	23.909141607456572	26.16456934400401	27.184897578453594	22.74139147008583
22-23	23.569255963737486	26.079053624017394	27.000000732398448	23.351689679846665
24-25	23.337280456526827	26.23356874511064	27.056504391382013	23.37264640698052
26-27	23.231874256199305	26.40369086244414	26.97853548260674	23.385899398749814
28-29	23.31252179764581	26.577563893640082	26.895271444564212	23.214642864149898
30-31	23.536213026898896	26.339235522284582	26.78365641366775	23.340895037148773
32-33	23.247882593233463	26.403770668424297	26.71479166614815	23.63355507219409
34-35	23.709360068145724	26.40173977122465	26.660682397039604	23.228217763590024
36-37	23.724737132965153	26.101325955123205	26.701449657257253	23.472487254654382
38-39	23.478711417665448	26.259104980024382	26.779548507772983	23.482635094537187
40-41	23.71586624194404	26.0587066455213	26.850838063005988	23.374589049528673
42-43	23.561587971807242	26.048764264969552	26.727411093284044	23.662236669939166
44-45	23.438352301923384	26.10167000304212	26.84725256288972	23.612725132144767
46-47	23.51840369764951	26.090492563633877	26.679560223328895	23.711543515387717
48-49	23.30818692650311	26.15277539926141	26.683352873760064	23.85568480047541
50-51	23.246871168050905	26.23845008367802	26.76265083891004	23.752027909361033
52-53	23.456196707077943	26.055737204392926	26.744420402949636	23.743645685579498
54-55	23.141617512673527	25.96313015048389	26.914378298655357	23.980874038187224
56-57	23.02559552524718	26.097494719192166	26.778233001887507	24.09867675367315
58-59	23.007157976329765	25.911432300730436	26.81548064231809	24.26592908062171
60-61	22.948596443920557	25.89492675017413	26.94480139528497	24.21167541062034
62-63	22.90314643485969	25.858082337671956	26.918432978512097	24.320338248956254
64-65	22.75491031379961	25.821493511730438	26.78295498938174	24.640641185088214
66-67	22.725908381848793	25.92325787265245	26.641348142590786	24.709485602907968
68-69	22.910367017176455	25.778129091628756	26.610085669861792	24.701418221333
70-71	22.67739088465869	25.6744442328319	26.638265367889385	25.009899514620027
72-73	22.710610320884204	25.697136514047276	26.479598059762445	25.112655105306075
74-75	22.61674661466714	25.88060841436653	26.33826536837774	25.16437960258859
76-77	22.582260809556484	25.74687896057728	26.537340317079444	25.133519912786795
78-79	22.520501885152534	25.813253528225438	26.463290973487464	25.202953613134564
80-81	22.547930210352337	25.827134911321075	26.29830915664523	25.32662572168135
82-83	22.454010952240715	25.860382643397596	26.351479922550926	25.334126481810763
84-85	22.361801211551676	25.853227620802038	26.236292830444974	25.548678337201313
86-87	22.311359183524935	25.85906437348053	26.232968092062396	25.596608350932136
88-89	22.2528608668758	25.813723324349404	26.111047290829028	25.82236851794577
90-91	22.11308351950474	25.786296894025458	26.150118116054433	25.950501470415364
92-93	22.184383404585887	25.662199300247885	26.14128318634113	26.012134108825098
94-95	21.929550588405412	25.638174650868915	26.22065254267304	26.21162221805263
96-97	22.026997695233025	25.41382107883351	26.21126137802145	26.347919847912017
98-99	21.89996430234204	25.526372138790272	26.047669180041332	26.525994378826358
100-101	21.979569580386467	25.440984926956116	26.08816434613246	26.491281146524955
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	19.5
2	41.0
3	71.0
4	69.0
5	68.5
6	116.5
7	244.5
8	366.0
9	403.5
10	383.5
11	371.5
12	391.5
13	425.5
14	470.5
15	543.5
16	650.5
17	721.0
18	804.5
19	940.5
20	1085.5
21	1300.0
22	1635.0
23	2000.5
24	2502.5
25	3312.5
26	4623.5
27	6594.5
28	9246.5
29	13162.5
30	18686.0
31	25983.5
32	34953.0
33	42112.5
34	48667.0
35	56807.5
36	66143.0
37	76329.5
38	85095.0
39	92746.5
40	100777.0
41	107914.5
42	114064.0
43	121529.5
44	128808.5
45	135585.5
46	141685.5
47	145545.0
48	147760.5
49	148877.5
50	148279.0
51	146137.0
52	143460.5
53	141116.5
54	136578.0
55	127311.5
56	115735.5
57	103557.0
58	92546.0
59	80393.0
60	66381.5
61	53897.5
62	43258.5
63	32996.0
64	23934.0
65	17688.0
66	13218.5
67	10152.5
68	7537.5
69	5402.5
70	4005.5
71	2960.5
72	2171.0
73	1637.0
74	1215.0
75	853.5
76	565.5
77	319.5
78	166.5
79	88.0
80	50.5
81	25.0
82	12.0
83	10.0
84	6.5
85	3.0
86	1.5
87	0.5
88	0.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1867213844136381
2	0.0
3	0.013054983139752717
4	0.013230610715623828
5	0.00632259273136006
6	0.10704500749344323
7	0.14146801236418133
8	0.1232320157362308
9	0.14190708130385912
10-11	0.11428964499812665
12-13	0.1151824185088048
14-15	0.11671915979767702
16-17	0.06710436961408767
18-19	0.08093504121393781
20-21	0.037803835706257026
22-23	0.08422805826152116
24-25	0.05909867928062945
26-27	0.06635795241663543
28-29	0.04059924128887224
30-31	0.02529037092544024
32-33	0.03268136474334957
34-35	0.020445976957662045
36-37	0.02510010771824653
38-39	0.033837579617834394
40-41	0.02676856968902211
42-43	0.014006299175721245
44-45	0.02742717309853878
46-47	0.034466911764705885
48-49	0.009513160359685274
50-51	0.016055287560884226
52-53	0.006893382352941176
54-55	0.012864719932559013
56-57	0.005151742225552642
58-59	0.0038345354065192957
60-61	0.023914621581116523
62-63	0.010962087860621956
64-65	0.007917876545522668
66-67	0.041828634319970026
68-69	0.09077018546272012
70-71	0.02190954008992132
72-73	0.04635104439865118
74-75	0.08651121674784563
76-77	0.23769728831022854
78-79	0.07816890689396777
80-81	0.0175188506931435
82-83	0.011649962532783815
84-85	0.013245246346946422
86-87	0.26028006744098914
88-89	0.6255268827276134
90-91	0.2719153943424504
92-93	0.3546067113150993
94-95	0.0015513769201948298
96-97	0.004083341139003372
98-99	0.0037906285125515176
100-101	0.005517633008617459
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3416320.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.4569035012059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25995264882309	42.66746933347548
2	13.602703752638682	15.631384737417042
3	5.133206783370169	8.84814500411506
4	2.4423356968808596	5.613161858129361
5	1.4000983196962613	4.022265702349431
6	0.8008527570433237	2.7608711748067765
7	0.5440610347669039	2.1882043661357766
8	0.3505113159235033	1.611143588407794
9	0.25871395262771957	1.3378412349491795
>10	1.1402662143712823	11.060632078733315
>50	0.04990456876044501	1.938255272569108
>100	0.01580877716219939	1.5245358546670187
>500	0.0010220502846762193	0.39289188138636477
>1k	5.621276509560499E-4	0.4031979128583263
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	5.854252529037092E-5	0.0	5.854252529037092E-5	0.0
5	0.0	5.854252529037092E-5	0.0	5.854252529037092E-5	0.0
6	0.0	5.854252529037092E-5	0.0	5.854252529037092E-5	0.0
7	0.0	5.854252529037092E-5	0.0	5.854252529037092E-5	0.0
8	0.0	5.854252529037092E-5	0.0	5.854252529037092E-5	0.0
9	0.0	5.854252529037092E-5	0.0	8.781378793555638E-5	0.0
10-11	0.0	5.854252529037092E-5	0.0	8.781378793555638E-5	0.0
12-13	0.0	5.854252529037092E-5	0.0	1.3172068190333457E-4	7.317815661296366E-5
14-15	0.0	5.854252529037092E-5	0.0	1.463563132259273E-4	1.1708505058074185E-4
16-17	0.0	5.854252529037092E-5	0.0	1.6099194454852005E-4	1.6099194454852005E-4
18-19	0.0	5.854252529037092E-5	0.0	2.341701011614837E-4	2.341701011614837E-4
20-21	0.0	8.781378793555638E-5	0.0	2.341701011614837E-4	2.341701011614837E-4
22-23	0.0	8.781378793555638E-5	0.0	4.097976770325965E-4	2.341701011614837E-4
24-25	0.0	1.0244941925814912E-4	0.0	7.610528287748221E-4	2.341701011614837E-4
26-27	0.0	1.1708505058074185E-4	0.0	0.0016099194454852004	2.341701011614837E-4
28-29	0.0	1.1708505058074185E-4	0.0	0.007390993817909329	2.341701011614837E-4
30-31	0.0	1.902632071937055E-4	0.0	0.033603409516672914	2.341701011614837E-4
32-33	0.0	3.0734825777444733E-4	0.0	0.08974569127013862	2.341701011614837E-4
34-35	0.0	3.219838890970401E-4	0.0	0.16339218808542524	2.927126264518546E-4
36-37	0.0	3.366195204196328E-4	0.0	0.28715108654926935	2.927126264518546E-4
38-39	0.0	4.097976770325965E-4	0.0	0.4932793180966654	2.927126264518546E-4
40-41	0.0	4.097976770325965E-4	0.0	0.732162092544024	3.0734825777444733E-4
42-43	0.0	4.2443330835518925E-4	0.0	0.9871147901835893	3.219838890970401E-4
44-45	0.0	4.3906893967778196E-4	0.0	1.2976243443237168	3.219838890970401E-4
46-47	0.0	4.3906893967778196E-4	0.0	1.6557728784188837	3.219838890970401E-4
48-49	0.0	4.829758336455601E-4	0.0	2.03559678250281	3.219838890970401E-4
50-51	0.0	4.976114649681529E-4	0.0	2.4408427781940802	3.512551517422255E-4
52-53	0.0	4.976114649681529E-4	0.0	2.8611195672536534	3.80526414387411E-4
54-55	0.0	4.976114649681529E-4	0.0	3.3330162279880104	4.097976770325965E-4
56-57	1.463563132259273E-5	4.976114649681529E-4	0.0	3.863045616335706	4.2443330835518925E-4
58-59	2.927126264518546E-5	4.976114649681529E-4	0.0	4.425771005058074	4.537045710003747E-4
60-61	2.927126264518546E-5	4.976114649681529E-4	0.0	5.027456444361184	4.683402023229674E-4
62-63	2.927126264518546E-5	4.976114649681529E-4	0.0	5.638406238291495	4.976114649681529E-4
64-65	2.927126264518546E-5	5.268827276133383E-4	0.0	6.29736090295991	5.268827276133383E-4
66-67	2.927126264518546E-5	5.561539902585238E-4	0.0	7.039504496065943	5.268827276133383E-4
68-69	2.927126264518546E-5	5.561539902585238E-4	0.0	7.8403516064068945	5.415183589359311E-4
70-71	2.927126264518546E-5	5.561539902585238E-4	0.0	8.678314677781941	5.561539902585238E-4
72-73	2.927126264518546E-5	5.561539902585238E-4	0.0	9.530840202322967	5.707896215811166E-4
74-75	2.927126264518546E-5	5.561539902585238E-4	0.0	10.415959277819407	5.854252529037092E-4
76-77	2.927126264518546E-5	5.561539902585238E-4	0.0	11.367392398838517	6.000608842263019E-4
78-79	2.927126264518546E-5	5.561539902585238E-4	0.0	12.387920335331586	6.146965155488947E-4
80-81	2.927126264518546E-5	5.561539902585238E-4	0.0	13.451418485387785	6.146965155488947E-4
82-83	2.927126264518546E-5	5.561539902585238E-4	0.0	14.523610200449607	6.439677781940802E-4
84-85	2.927126264518546E-5	5.561539902585238E-4	0.0	15.597119122330461	6.439677781940802E-4
86-87	2.927126264518546E-5	5.561539902585238E-4	0.0	16.7227016204571	6.58603409516673E-4
88-89	2.927126264518546E-5	5.561539902585238E-4	0.0	17.905202088797303	6.732390408392656E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2595	0.0	44.336876	1
GTATCAA	4805	0.0	43.832615	1
TCAACGC	5800	0.0	35.43508	4
CAACGCA	5970	0.0	34.344017	5
ATCAACG	6030	0.0	34.00478	3
AACGCAG	6465	0.0	31.861258	6
TATCAAC	6535	0.0	31.447807	2
ACGCAGA	8110	0.0	25.398647	7
CGCAGAG	8195	0.0	24.786623	8
GTACATG	9210	0.0	23.02302	1
TACATGG	9225	0.0	22.689259	2
ACATGGG	9645	0.0	20.718266	3
GCAGAGT	9630	0.0	20.00886	9
TAAGGCG	1120	0.0	19.069365	5
GGACCGT	410	1.8189894E-12	18.521572	6
GTAAGGT	1360	0.0	18.148386	4
CATGGGG	6715	0.0	18.024687	4
TAAGGTG	1705	0.0	17.81547	5
GAGTACA	7040	0.0	17.761112	1
GTATAGG	830	0.0	17.757044	1
>>END_MODULE