FastQCFastQC Report
Mon 15 Aug 2016
SRR2920861_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920861_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3978438
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58500.041.5378651
TCAACGC66200.035.950534
GGTATCA34550.035.831941
ATCAACG66850.035.5323753
CAACGCA67550.035.02845
TATCAAC71450.033.4392
AACGCAG73200.032.4438746
ACGCAGA90150.025.3749477
GTACATG106950.024.226671
CGCAGAG93450.024.0843148
TACATGG111000.022.5573162
ACATGGG114600.021.6075293
GCAGAGT107100.020.0303489
GAGTACA83450.019.3573951
AGTACAT80700.018.4115032
CATGGGG82000.018.1187024
GTATATG17550.017.3074441
GTGTAGC24700.017.1418231
AGAGTAC87550.017.016510-11
GTATAGT10750.016.6963791
Produced by FastQC (version 0.11.5)