FastQCFastQC Report
Mon 15 Aug 2016
SRR2920861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3978438
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57300.042.327021
GGTATCA34800.040.8595961
TCAACGC71750.032.745244
ATCAACG72450.032.4943773
CAACGCA73250.032.135045
TATCAAC76550.031.1860492
AACGCAG78600.029.9477316
GTACATG118500.025.6840651
TACATGG120100.024.7777632
ACGCAGA94000.024.385077
ACATGGG121950.023.1968693
CGCAGAG98150.022.9174088
GAGTACA91300.020.2619361
GCAGAGT112250.019.4888679
CATGGGG84600.019.075394
AGTACAT89900.018.7943742
GTATATA15750.018.720321
AGAGTAC94950.018.06617410-11
GTATAGC14950.017.8134881
TATACCG2355.179001E-516.1557435
Produced by FastQC (version 0.11.5)