##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3978438
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.327790454444685	31.0	30.0	34.0	27.0	34.0
2	30.341845216640298	31.0	30.0	34.0	26.0	34.0
3	30.48271432155032	31.0	30.0	34.0	27.0	34.0
4	34.12616157396445	35.0	35.0	37.0	30.0	37.0
5	33.66484735969242	35.0	35.0	37.0	28.0	37.0
6	33.609349950910385	35.0	35.0	37.0	28.0	37.0
7	33.437168054397226	35.0	35.0	37.0	28.0	37.0
8	33.36788081151447	35.0	35.0	37.0	27.0	37.0
9	34.71721967264539	38.0	35.0	39.0	27.0	39.0
10-11	34.53230740305617	38.0	34.0	39.0	27.0	39.0
12-13	34.35166879564291	37.0	34.0	39.0	27.0	39.0
14-15	35.26580381546728	38.5	34.0	40.0	27.0	41.0
16-17	35.14002555776915	38.0	34.0	40.0	26.0	41.0
18-19	35.05269542971387	38.5	34.0	40.0	25.5	41.0
20-21	34.938819204924144	38.5	34.0	40.0	25.0	41.0
22-23	34.798699012024315	38.0	34.0	40.0	25.0	41.0
24-25	34.62980735152841	38.0	33.5	40.0	24.0	41.0
26-27	34.45104108697936	38.0	33.0	40.0	24.0	41.0
28-29	34.396330670479216	38.0	33.0	40.0	24.0	41.0
30-31	34.398512808293106	38.0	33.0	40.0	24.0	41.0
32-33	34.25653686195436	38.0	33.0	40.0	23.0	41.0
34-35	34.09948653215156	38.0	33.0	40.0	22.5	41.0
36-37	33.91432894015189	38.0	33.0	40.0	21.5	41.0
38-39	33.739470365002546	38.0	33.0	40.0	19.5	41.0
40-41	33.52163688362116	38.0	32.0	40.0	18.5	41.0
42-43	33.565735220707225	38.0	33.0	40.0	18.5	41.0
44-45	33.43041565559146	38.0	32.0	40.0	18.0	41.0
46-47	33.19906857917605	38.0	32.0	40.0	15.5	41.0
48-49	32.9535454618119	37.5	32.0	40.0	14.5	41.0
50-51	32.705034865442165	37.0	31.0	40.0	12.5	41.0
52-53	32.45549861528569	37.0	31.0	40.0	9.0	41.0
54-55	32.18662110607228	37.0	31.0	40.0	8.5	41.0
56-57	31.870573576866096	36.0	30.5	39.5	7.5	41.0
58-59	31.554210345869407	36.0	30.0	39.0	6.5	41.0
60-61	31.232073994869342	35.5	29.5	39.0	2.0	40.0
62-63	30.913535035609453	35.0	29.0	39.0	2.0	40.0
64-65	30.518295873908304	35.0	29.0	38.5	2.0	40.0
66-67	30.09100858175998	34.5	28.0	38.0	2.0	40.0
68-69	29.647915714659874	34.0	28.0	37.5	2.0	40.0
70-71	29.215064429808884	34.0	28.0	37.0	2.0	39.0
72-73	28.766785230786553	34.0	26.5	36.0	2.0	39.0
74-75	28.317796833832777	34.0	26.0	36.0	2.0	39.0
76-77	26.837884239995695	31.5	25.0	34.5	2.0	36.5
78-79	27.67619276711111	33.0	26.0	35.0	2.0	37.0
80-81	27.69122215804293	33.0	26.0	35.0	2.0	37.0
82-83	27.340310192090463	33.0	26.0	35.0	2.0	36.0
84-85	27.00924219505243	33.0	25.5	35.0	2.0	36.0
86-87	26.455438290102798	33.0	25.0	35.0	2.0	35.5
88-89	26.081363841789162	32.0	23.5	35.0	2.0	35.0
90-91	25.70616445449194	32.0	22.5	35.0	2.0	35.0
92-93	25.39035483272581	32.0	20.0	34.0	2.0	35.0
94-95	25.002792678935805	32.0	19.0	34.0	2.0	35.0
96-97	24.472201904365484	32.0	12.0	34.0	2.0	35.0
98-99	23.861942802677834	31.5	2.0	34.0	2.0	35.0
100-101	21.6499552085517	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87093.0
3	40132.0
4	21794.0
5	15513.0
6	12921.0
7	13109.0
8	14317.0
9	16980.0
10	19239.0
11	21640.0
12	24105.0
13	26433.0
14	28657.0
15	30489.0
16	32106.0
17	33507.0
18	32836.0
19	32238.0
20	32487.0
21	33972.0
22	36734.0
23	41627.0
24	48467.0
25	57143.0
26	67257.0
27	80057.0
28	94706.0
29	111665.0
30	132150.0
31	155511.0
32	186850.0
33	228839.0
34	288439.0
35	374828.0
36	500527.0
37	606362.0
38	371300.0
39	26407.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.627602481155854	19.03984909575821	13.38912492161391	24.94342350147202
2	16.053787943911658	20.29713671546471	38.64873601146983	25.000339329153803
3	19.690315931044452	23.82631648409661	28.958640497244165	27.524727087614774
4	13.74347356203425	16.010949608822234	35.962461213431645	34.283115615711864
5	14.484485289681661	36.94253122046373	34.23700906951957	14.33597442033503
6	34.14036442967121	35.06521512120187	16.811347317857507	13.983073131269414
7	29.66348971097288	31.287996101466582	20.47188162634893	18.576632561211607
8	26.535207481964274	35.340261562722254	19.525068818387407	18.599462136926068
9	26.37856627747901	16.08075402643724	19.21549132162288	38.325188374460865
10-11	25.828552663282505	25.50085212206389	27.45531627914877	21.215278935504834
12-13	26.24304661784195	22.539898393897843	27.487323143364705	23.729731844895493
14-15	23.224692874739247	24.3034138506047	24.91785939823996	27.554033876416096
16-17	23.67582221200477	27.392147280186553	25.579126956319648	23.35290355148903
18-19	23.555732594632612	26.70071552408542	26.959817551923543	22.783734329358424
20-21	24.204470219699008	25.940641312003372	26.80685984529315	23.048028623004466
22-23	23.74374855348823	25.79994616460549	26.622143453716657	23.83416182818962
24-25	23.58184330881641	26.00637800493692	26.685990143769185	23.72578854247749
26-27	23.54163268524554	26.171351376299434	26.64291394149817	23.644101996956856
28-29	23.53236192774732	26.40165618558814	26.46240780074878	23.60357408591576
30-31	23.862121711338876	26.142812372444507	26.39528661118452	23.599779305032097
32-33	23.5601085201929	26.145270219303317	26.380099770186614	23.91452149031716
34-35	23.876197401839917	26.08347071648956	26.507983097943562	23.53234878372696
36-37	23.884668090272196	25.98595912793078	26.45284006034505	23.676532721451974
38-39	23.785531885791066	26.100504081835858	26.4817335708244	23.632230461548673
40-41	23.956947652470674	25.867663020181087	26.587485860869702	23.587903466478537
42-43	23.693267147624198	25.834712202273664	26.494633597945448	23.977387052156693
44-45	23.64598585062741	25.93116345445629	26.614985683304926	23.807865011611376
46-47	23.622802261111538	25.80757620464611	26.562252290976485	24.007369243265867
48-49	23.489653587873992	25.938288537173833	26.577577100993594	23.994480773958585
50-51	23.543333799668638	26.028058804504823	26.5610385777452	23.86756881808134
52-53	23.611881289663497	25.884040688218708	26.528824675967797	23.975253346149998
54-55	23.405408489991405	25.690591415487095	26.706697526930455	24.19730256759104
56-57	23.362567009989238	25.877842956097453	26.58153503635975	24.17805499755356
58-59	23.273215149782256	25.820801729392766	26.645304811758862	24.260678309066115
60-61	23.369861653309265	25.763835769008608	26.687228119620137	24.17907445806199
62-63	23.23229098426798	25.839139849602894	26.585469288351288	24.34309987777784
64-65	23.00566834755961	25.650670972527763	26.61691314051391	24.726747539398716
66-67	23.007537193108448	25.856200896597574	26.465017707651256	24.67124420264272
68-69	23.18293493059227	25.76424102840652	26.3646974779546	24.688126563046605
70-71	22.970326029022818	25.540846649704847	26.45737587867939	25.031451442592946
72-73	22.926250463486213	25.661966443912476	26.37394664938917	25.037836443212143
74-75	22.868563501900752	25.740118193502553	26.249916037883708	25.14140226671299
76-77	22.852459772426492	25.611173966858786	26.466524557654513	25.06984170306021
78-79	22.81686980421969	25.65282533977356	26.355792545873552	25.174512310133196
80-81	22.7299975400694	25.731276276602795	26.250488812218048	25.288237371109755
82-83	22.74271810491238	25.725135170193276	26.261064749966568	25.271081974927778
84-85	22.701483742484125	25.640913805577497	26.192421455064135	25.465180996874242
86-87	22.49592232769905	25.64589031247389	26.2834223891335	25.574764970693565
88-89	22.54771408592336	25.577737343010863	26.20252573816812	25.672022832897657
90-91	22.39620020953133	25.61936367900123	26.161262006656916	25.823174104810526
92-93	22.436130277535202	25.557108544601228	26.120144849230577	25.886616328633
94-95	22.280869612975398	25.5182843413687	26.118929250409177	26.081916795246723
96-97	22.264461451295613	25.387480374615475	26.174368430869126	26.173689743219786
98-99	22.17807300151497	25.388048817824494	26.107375987890308	26.326502192770228
100-101	22.224720535895404	25.344631129502275	26.122387317604602	26.30826101699772
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	37.5
2	53.0
3	89.0
4	77.5
5	70.0
6	140.0
7	285.0
8	463.5
9	526.5
10	454.0
11	407.5
12	417.0
13	469.0
14	535.0
15	605.5
16	703.0
17	778.5
18	871.5
19	1007.5
20	1173.0
21	1373.0
22	1645.5
23	2071.5
24	2625.5
25	3239.0
26	4078.0
27	5352.5
28	7152.5
29	9833.5
30	14155.5
31	20472.0
32	28515.5
33	36908.5
34	45780.0
35	56673.5
36	69067.0
37	80839.0
38	92291.5
39	103416.0
40	114643.0
41	125810.0
42	136231.5
43	146654.0
44	155380.0
45	163951.5
46	171823.0
47	177267.0
48	183187.0
49	186018.5
50	184625.5
51	182182.5
52	177552.5
53	171265.5
54	162483.5
55	150216.0
56	135503.5
57	119007.0
58	104890.5
59	91763.5
60	76712.0
61	63187.0
62	51072.5
63	38549.5
64	28117.5
65	21332.0
66	16413.0
67	12639.0
68	9569.5
69	7024.5
70	5110.0
71	3876.0
72	2931.5
73	2227.0
74	1621.0
75	1139.0
76	759.0
77	450.0
78	260.5
79	149.5
80	86.5
81	40.5
82	13.5
83	8.0
84	4.5
85	1.5
86	1.5
87	2.0
88	1.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19374437907540598
2	0.0
3	0.012894507844535972
4	0.014553450374242355
5	0.006987667019066277
6	0.10765531598079447
7	0.14231716065450814
8	0.12406879282773793
9	0.14158823136115228
10-11	0.1140020279315651
12-13	0.11468068623917224
14-15	0.11591232539001486
16-17	0.06708663048161112
18-19	0.07967951241165502
20-21	0.03762783283288567
22-23	0.08440498507203079
24-25	0.057132975303372836
26-27	0.0656916106270853
28-29	0.03994029817732487
30-31	0.02519833160652497
32-33	0.032625869750892184
34-35	0.020497994439023556
36-37	0.024595079777540833
38-39	0.0328269536938869
40-41	0.025160628367213465
42-43	0.013573166152143127
44-45	0.027359983993718136
46-47	0.03437278650565875
48-49	0.008646609548772659
50-51	0.015420624878407055
52-53	0.007138479976312311
54-55	0.012743694887289936
56-57	0.005190479278551029
58-59	0.004097085338517277
60-61	0.023388576119572558
62-63	0.01095907489321186
64-65	0.007402402651492873
66-67	0.04087031141367542
68-69	0.09120413589453952
70-71	0.022068962743669752
72-73	0.04480401604850949
74-75	0.08623987605185754
76-77	0.2603408674459675
78-79	0.08086088057674896
80-81	0.017180109379610793
82-83	0.01231639150842617
84-85	0.0131709982661537
86-87	0.2713250778320537
88-89	0.6288774639695277
90-91	0.2648024174311627
92-93	0.3529023199557213
94-95	0.0011939359115311085
96-97	0.004323304774386329
98-99	0.00344356252378446
100-101	0.005001963081993486
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3978438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.99217717115416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.51901346631453	35.253990151430024
2	13.264426205920952	13.262350899202008
3	6.022453558972794	9.032266959756473
4	3.148097140223366	6.29520919944201
5	1.877132363931799	4.692096685569296
6	1.248382223917425	3.7445607189239607
7	0.8556479584499262	2.994299303447555
8	0.5944262896454969	2.3773331509719586
9	0.4388096332968933	1.9743344038968726
>10	1.9443872431072948	16.145445442816296
>50	0.06551377016391612	2.2260543604057896
>100	0.02100454689367836	1.6849667293119568
>500	5.039994164732728E-4	0.18012640026558402
>1k	2.0159974552107397E-4	0.1369655945602304
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5135492874339126E-5
2	0.0	0.0	0.0	0.0	2.5135492874339126E-5
3	0.0	0.0	0.0	0.0	2.5135492874339126E-5
4	0.0	0.0	0.0	0.0	2.5135492874339126E-5
5	0.0	0.0	0.0	0.0	2.5135492874339126E-5
6	0.0	0.0	0.0	0.0	7.540647862301737E-5
7	0.0	0.0	0.0	0.0	7.540647862301737E-5
8	0.0	0.0	0.0	2.5135492874339126E-5	1.005419714973565E-4
9	0.0	0.0	0.0	7.540647862301737E-5	1.5081295724603475E-4
10-11	0.0	0.0	0.0	8.797422506018694E-5	1.5081295724603475E-4
12-13	0.0	0.0	0.0	1.7594845012037388E-4	1.5081295724603475E-4
14-15	0.0	0.0	0.0	1.7594845012037388E-4	2.3878718230622168E-4
16-17	0.0	0.0	0.0	1.8851619655754344E-4	3.5189690024074776E-4
18-19	0.0	0.0	0.0	2.1365168943188257E-4	3.770323931150869E-4
20-21	0.0	0.0	0.0	3.1419366092923906E-4	3.770323931150869E-4
22-23	0.0	0.0	0.0	3.8960013955225645E-4	6.158195754213086E-4
24-25	0.0	0.0	2.5135492874339126E-5	5.529808432354607E-4	8.671745041646999E-4
26-27	0.0	0.0	2.5135492874339126E-5	0.0012819101365912954	8.797422506018694E-4
28-29	0.0	0.0	2.5135492874339126E-5	0.00545440195373159	9.048777434762086E-4
30-31	0.0	0.0	2.5135492874339126E-5	0.023765608512687643	9.174454899133781E-4
32-33	0.0	0.0	2.5135492874339126E-5	0.06640797217400396	9.802842220992258E-4
34-35	0.0	0.0	2.5135492874339126E-5	0.1253632707107664	0.0011059616864709215
36-37	0.0	0.0	2.5135492874339126E-5	0.22252451841652426	0.0011436649257824303
38-39	0.0	0.0	2.5135492874339126E-5	0.3863576609714667	0.0011939359115311085
40-41	0.0	0.0	2.5135492874339126E-5	0.5721340888057072	0.0012190714044054475
42-43	0.0	0.0	2.5135492874339126E-5	0.7687313463223506	0.0012819101365912954
44-45	0.0	0.0	2.5135492874339126E-5	1.0063748637027898	0.0013321811223399736
46-47	0.0	0.0	2.5135492874339126E-5	1.2877792741774536	0.0014201553474001605
48-49	0.0	0.0	2.5135492874339126E-5	1.595852442591791	0.0015081295724603476
50-51	0.0	1.2567746437169563E-5	2.5135492874339126E-5	1.9273393226185753	0.0015081295724603476
52-53	0.0	2.5135492874339126E-5	2.5135492874339126E-5	2.2578836216625717	0.001608671543957704
54-55	0.0	2.5135492874339126E-5	2.5135492874339126E-5	2.634551550130981	0.00172178126189223
56-57	0.0	2.5135492874339126E-5	2.5135492874339126E-5	3.073191036281073	0.0017469167547665694
58-59	0.0	2.5135492874339126E-5	2.5135492874339126E-5	3.5458137087972714	0.001784619994078078
60-61	0.0	2.5135492874339126E-5	2.5135492874339126E-5	4.051941993314965	0.001784619994078078
62-63	0.0	2.5135492874339126E-5	2.5135492874339126E-5	4.571693714970548	0.0018600264727010951
64-65	0.0	2.5135492874339126E-5	2.5135492874339126E-5	5.119861111320573	0.0018600264727010951
66-67	0.0	2.5135492874339126E-5	2.5135492874339126E-5	5.737063138849971	0.0018600264727010951
68-69	0.0	2.5135492874339126E-5	2.5135492874339126E-5	6.416098478850243	0.0018725942191382648
70-71	0.0	2.5135492874339126E-5	2.5135492874339126E-5	7.145568185302875	0.001922865204886943
72-73	0.0	2.5135492874339126E-5	2.5135492874339126E-5	7.878632267236538	0.0021616523871931647
74-75	0.0	2.5135492874339126E-5	2.5135492874339126E-5	8.63097527220482	0.002186787880067504
76-77	0.0	2.5135492874339126E-5	2.5135492874339126E-5	9.451096133708756	0.002186787880067504
78-79	0.0	2.5135492874339126E-5	2.5135492874339126E-5	10.339057690480535	0.002186787880067504
80-81	0.0	2.5135492874339126E-5	2.5135492874339126E-5	11.263566253891604	0.0022244911193790126
82-83	1.2567746437169563E-5	2.5135492874339126E-5	2.5135492874339126E-5	12.196444936429826	0.0023124653444391995
84-85	2.5135492874339126E-5	2.5135492874339126E-5	2.5135492874339126E-5	13.132830020224016	0.0023124653444391995
86-87	2.5135492874339126E-5	2.5135492874339126E-5	2.5135492874339126E-5	14.11517535273894	0.0023753040766250474
88-89	2.5135492874339126E-5	2.5135492874339126E-5	2.5135492874339126E-5	15.156149222383256	0.0024381428088108953
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5730	0.0	42.32702	1
GGTATCA	3480	0.0	40.859596	1
TCAACGC	7175	0.0	32.74524	4
ATCAACG	7245	0.0	32.494377	3
CAACGCA	7325	0.0	32.13504	5
TATCAAC	7655	0.0	31.186049	2
AACGCAG	7860	0.0	29.947731	6
GTACATG	11850	0.0	25.684065	1
TACATGG	12010	0.0	24.777763	2
ACGCAGA	9400	0.0	24.38507	7
ACATGGG	12195	0.0	23.196869	3
CGCAGAG	9815	0.0	22.917408	8
GAGTACA	9130	0.0	20.261936	1
GCAGAGT	11225	0.0	19.488867	9
CATGGGG	8460	0.0	19.07539	4
AGTACAT	8990	0.0	18.794374	2
GTATATA	1575	0.0	18.72032	1
AGAGTAC	9495	0.0	18.066174	10-11
GTATAGC	1495	0.0	17.813488	1
TATACCG	235	5.179001E-5	16.155743	5
>>END_MODULE