FastQCFastQC Report
Mon 15 Aug 2016
SRR2920860_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920860_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859974
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51000.044.0382651
GGTATCA29000.037.612391
TCAACGC63700.034.9127854
ATCAACG65450.034.2595253
CAACGCA65150.034.2061355
TATCAAC65900.033.8180242
AACGCAG68450.032.7528576
GTACATG101900.028.2735771
ACGCAGA82300.026.5126137
TACATGG107100.026.1178682
CGCAGAG84200.025.4765838
ACATGGG109400.024.7715023
CATGGGG72250.021.0708684
GCAGAGT94000.021.0166979
GTATAGG4650.018.805341
GAGTACA75950.018.6639481
AGAGTAC77150.018.38886810-11
GTACGAA2302.7854567E-618.0092331
TAAGGTA5650.017.8548564
TACACCG3702.3464963E-1017.350365
Produced by FastQC (version 0.11.5)