##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920860_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2859974
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.62138362096998	31.0	30.0	33.0	22.0	34.0
2	28.6349791991116	31.0	28.0	34.0	18.0	34.0
3	28.71086800089791	31.0	28.0	34.0	22.0	34.0
4	32.191847198610894	35.0	32.0	37.0	22.0	37.0
5	32.15557239331547	35.0	32.0	37.0	22.0	37.0
6	32.28440957854862	35.0	32.0	37.0	23.0	37.0
7	32.11505279418624	35.0	32.0	37.0	22.0	37.0
8	32.05795507231884	35.0	32.0	37.0	22.0	37.0
9	33.324707846994414	37.0	33.0	39.0	22.0	39.0
10-11	33.1282130536851	37.0	32.0	39.0	19.0	39.0
12-13	32.95121424180779	37.0	32.0	39.0	18.0	39.0
14-15	33.710760482437955	38.0	32.0	40.0	17.0	41.0
16-17	33.59930876993987	38.0	32.0	40.0	17.0	41.0
18-19	33.46919622346218	38.0	32.0	40.0	17.5	41.0
20-21	33.369105278579454	38.0	32.0	40.0	17.0	41.0
22-23	33.14910065615981	38.0	32.0	40.0	16.0	41.0
24-25	32.95231844765023	38.0	32.0	40.0	12.5	41.0
26-27	32.98526927167869	38.0	32.0	40.0	10.5	41.0
28-29	32.82029696773468	38.0	31.5	40.0	10.0	41.0
30-31	32.56987336248511	38.0	31.0	40.0	9.0	41.0
32-33	32.32920561515594	37.0	30.5	40.0	8.5	41.0
34-35	32.00737996219546	37.0	30.0	40.0	7.0	41.0
36-37	31.705243998721667	37.0	30.0	40.0	2.0	41.0
38-39	31.499679018061002	37.0	30.0	40.0	2.0	41.0
40-41	31.28993742600457	37.0	30.0	40.0	2.0	41.0
42-43	31.05107371605476	36.0	29.5	40.0	2.0	41.0
44-45	30.769072900662735	36.0	29.0	39.0	2.0	41.0
46-47	30.50531246088251	36.0	28.0	39.0	2.0	40.0
48-49	30.19369179579954	35.5	28.0	39.0	2.0	40.0
50-51	29.033053622165795	34.0	25.5	38.0	2.0	39.5
52-53	29.47838564266668	34.5	27.5	38.5	2.0	39.5
54-55	30.120080637096702	35.0	28.0	39.0	2.0	40.0
56-57	30.058036017110645	35.0	28.0	39.0	2.0	40.0
58-59	29.797341339466723	35.0	28.0	39.0	2.0	40.0
60-61	29.453806747893513	35.0	27.0	39.0	2.0	40.0
62-63	29.051005358789975	34.5	26.0	38.0	2.0	40.0
64-65	28.614455411133108	34.0	26.0	38.0	2.0	40.0
66-67	28.223498010821075	34.0	26.0	37.0	2.0	40.0
68-69	27.768845625869325	34.0	25.0	37.0	2.0	39.0
70-71	27.440248407852657	33.5	25.0	36.0	2.0	39.0
72-73	26.981222032088404	33.0	23.5	36.0	2.0	39.0
74-75	26.53973217938345	33.0	22.5	35.0	2.0	38.0
76-77	26.13192305244733	32.5	21.5	35.0	2.0	37.0
78-79	25.675878521972578	32.0	20.0	35.0	2.0	36.5
80-81	25.225786143510398	32.0	18.0	35.0	2.0	36.0
82-83	24.753570487004424	32.0	15.0	35.0	2.0	36.0
84-85	24.35432839599241	31.0	8.5	34.0	2.0	35.0
86-87	23.89390462990223	31.0	4.5	34.0	2.0	35.0
88-89	23.35089007802169	31.0	2.0	34.0	2.0	35.0
90-91	22.84173527451648	30.5	2.0	34.0	2.0	35.0
92-93	22.296288532692955	30.0	2.0	34.0	2.0	35.0
94-95	21.64746008180494	30.0	2.0	34.0	2.0	35.0
96-97	20.987085022451254	29.0	2.0	34.0	2.0	35.0
98	20.581191297543263	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	144632.0
3	25178.0
4	19126.0
5	15658.0
6	14431.0
7	14749.0
8	16588.0
9	19205.0
10	21091.0
11	22505.0
12	22994.0
13	22877.0
14	21499.0
15	22349.0
16	23923.0
17	25464.0
18	26373.0
19	26414.0
20	27148.0
21	29340.0
22	31824.0
23	35637.0
24	40746.0
25	46687.0
26	53920.0
27	62414.0
28	72582.0
29	84214.0
30	97268.0
31	114771.0
32	136027.0
33	165435.0
34	204855.0
35	260337.0
36	328202.0
37	349447.0
38	195383.0
39	18678.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.32835822467397	17.082610605069682	12.339406883637853	25.249624286618495
2	16.604114620111297	18.992074409130606	38.534194127837004	25.86961684292109
3	20.25637537372338	22.706329052833095	27.934452005857167	29.102843567586355
4	13.775791402632647	14.662421139507883	34.9290780141844	36.63270944367507
5	15.09978096137079	36.28857701133104	33.31076380934861	15.300878217949556
6	35.175489768168006	33.26772314458863	16.764347507372403	14.792439579870962
7	30.772273266624538	30.50918467014957	19.815855468084727	18.902686595141166
8	27.662484351286516	33.62256352153752	19.352895099417413	19.362057027758546
9	27.3852489568087	15.439930572795419	18.644435229131453	38.53038524126443
10-11	26.53571790088265	24.566398954395908	26.68942561880381	22.208457525917634
12-13	27.06121530547858	21.750563282495854	26.770897749999605	24.417323662025954
14-15	24.136189326948866	23.436608893381734	24.12590909265743	28.301292687011976
16-17	24.519910286742537	26.41481261832212	24.730713363065245	24.334563731870094
18-19	24.418792355721866	25.72406870972769	26.178020085721887	23.67911884882856
20-21	25.274504736677546	24.861315071685738	25.914716883281404	23.94946330835532
22-23	24.849152719451094	24.673660793427207	25.77953293037391	24.697653556747785
24-25	24.593309504644704	24.83392899223401	25.852441857434087	24.72031964568719
26-27	24.43419438606056	25.122722802445203	25.8062647985145	24.636818012979738
28-29	24.493149985200695	25.304802742385	25.687013206019643	24.51503406639466
30-31	24.70547076573228	25.136764770192855	25.539493593387853	24.618270870687017
32-33	24.451815972161505	25.18346827076084	25.480965953800688	24.88374980327697
34-35	24.790890868499197	25.13316753731507	25.553008671394924	24.52293292279081
36-37	24.906032680800084	24.899370949790587	25.479363398656968	24.715232970752364
38-39	24.750116348547746	25.014981687292	25.513211731166248	24.721690232994007
40-41	24.798553906870858	25.0401960968628	25.567546397918427	24.593703598347915
42-43	24.80928343324064	24.83070782139436	25.48854190355689	24.87146684180811
44-45	24.5214062322791	24.96826697106966	25.636003636262767	24.874323160388474
46-47	24.713529551180205	24.863545790503945	25.571316965265407	24.851607693050447
48-49	24.601484383777102	24.92402539040557	25.488656471183013	24.98583375463431
50-51	24.38098625123841	25.16691815270457	25.61831160067996	24.833783995377058
52-53	24.61935473898849	24.93036982287755	25.701680907692616	24.748594530441345
54-55	24.285904739053706	24.902203213913904	25.861192236399216	24.95069981063317
56-57	24.296272493573266	24.913537584109456	25.77768763959712	25.012502282720163
58-59	24.283223720017276	24.657974851556766	25.865834003420073	25.19296742500588
60-61	24.247626064666843	24.653123965096242	25.991936047469228	25.107313922767688
62-63	24.169892669817443	24.72444160198421	25.96557576234239	25.14008996585595
64-65	24.108846137430156	24.70359566938172	25.877497509801394	25.310060683386727
66-67	24.08783399179488	24.814706671895006	25.785884748849973	25.31157458746014
68-69	24.203679626506585	24.564478620082998	25.796504732315377	25.435337021095037
70-71	24.01651845338697	24.49673298969615	25.92223058792145	25.564517968995432
72-73	24.053730224409392	24.59357340245797	25.83480560100458	25.51789077212806
74-75	24.071507974493972	24.752652433333004	25.85568081812427	25.32015877404875
76-77	24.043321046287833	24.570430034896145	25.94959094840669	25.436657970409332
78-79	24.099729795687924	24.650331829703035	25.800222149665576	25.44971622494347
80-81	24.07829387066389	24.62290889029579	25.896894271303456	25.401902967736863
82-83	24.101509703381126	24.605282251067212	25.944189701043953	25.349018344507712
84-85	24.149103271988665	24.63268296894071	25.836631477879912	25.381582281190717
86-87	24.131032578317683	24.587652541454048	25.936108644217487	25.34520623601078
88-89	24.00655588429756	24.492367411048786	25.841368030094724	25.659708674558935
90-91	24.09479103111252	24.48877099204091	25.951947149269305	25.46449082757726
92-93	24.26967577524188	24.39316423473826	25.872850049777885	25.464309940241975
94-95	24.20749319994027	24.328482586038895	25.979535860499503	25.484488353521336
96-97	24.391465790146484	24.12867818854133	25.929920849623763	25.549935171688425
98	24.397954925973274	24.081697682359394	25.882981310572546	25.637366081094786
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	67.0
1	59.0
2	72.0
3	105.0
4	117.5
5	134.0
6	219.5
7	414.5
8	631.5
9	744.0
10	757.0
11	827.5
12	988.0
13	1105.5
14	1232.5
15	1436.0
16	1567.0
17	1614.5
18	1634.5
19	1615.5
20	1671.0
21	1699.0
22	1744.0
23	1935.0
24	2171.0
25	2538.0
26	3170.0
27	4103.5
28	5243.5
29	7078.0
30	10117.0
31	14558.0
32	19420.5
33	23983.0
34	29767.0
35	36908.0
36	43789.0
37	50302.0
38	56771.0
39	63195.0
40	69133.0
41	74692.5
42	82314.0
43	90379.5
44	97291.0
45	105842.0
46	114684.0
47	121904.0
48	128027.5
49	131386.5
50	131986.0
51	133089.5
52	135498.0
53	137794.0
54	135998.5
55	130232.5
56	122309.0
57	111840.5
58	101342.5
59	90260.0
60	77180.0
61	63689.5
62	51969.0
63	40652.0
64	30151.5
65	22619.5
66	16870.5
67	12414.5
68	9146.0
69	6671.5
70	4859.0
71	3697.0
72	2908.5
73	2299.0
74	1785.0
75	1323.0
76	915.0
77	546.5
78	316.5
79	173.0
80	99.0
81	63.0
82	36.5
83	19.5
84	11.5
85	10.0
86	9.5
87	8.5
88	6.5
89	4.0
90	5.0
91	4.5
92	2.5
93	1.5
94	4.5
95	6.0
96	3.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.39836026481359615
2	0.026398841388068563
3	0.020035147172666603
4	0.01727288429894817
5	0.023112098221871945
6	0.02314706357470383
7	0.01681833471213375
8	0.010979120789209972
9	0.0
10-11	1.7482676415939444E-5
12-13	0.0061014540691628665
14-15	0.00409094628132983
16-17	0.006853209155048262
18-19	0.03767516767634951
20-21	0.08138185871619812
22-23	0.10008832248125334
24-25	0.06764047505326971
26-27	0.07736084314053204
28-29	0.060559991104814234
30-31	0.05590959917817434
32-33	0.02181838016709243
34-35	0.3526780313387464
36-37	0.5373475423203148
38-39	0.4894974569698885
40-41	0.4978192109438757
42-43	0.44586069663570366
44-45	0.4194094072183873
46-47	0.4178010009881209
48-49	0.40748622190271655
50-51	0.4046190629705025
52-53	0.46600073986686597
54-55	0.46792383427261924
56-57	0.43685711828149487
58-59	0.42252132362042455
60-61	0.4366123608116717
62-63	0.41586042390595157
64-65	0.46551122492721964
66-67	0.487626810593383
68-69	0.42890250051224243
70-71	0.3859825299111111
72-73	0.37161176989720884
74-75	0.3544787470095882
76-77	0.3527654447208261
78-79	0.3529227888085696
80-81	0.3681851653196847
82-83	0.36274805295432755
84-85	0.36335994662888543
86-87	0.3500381471999396
88-89	0.3928532217425753
90-91	0.3703005691660134
92-93	0.36066761446083073
94-95	0.36890195505273826
96-97	0.3679928558791094
98	0.3634648426873811
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2859974.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.910305281358426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5097844273372	35.464756922265515
2	11.583925391721275	11.331466545311342
3	5.440717937354587	7.983215257973267
4	2.9552768648305574	5.781739745991936
5	1.8770969426861486	4.590469225474204
6	1.260234835333814	3.698308231342758
7	0.8628587854609217	2.9541880628117085
8	0.6660448050841639	2.6061163798183453
9	0.503922260418583	2.2182292435630586
>10	2.2328462805758518	18.10120057704549
>50	0.07913275920477587	2.6000492967079727
>100	0.027221999789074965	2.304693793612025
>500	7.205463099899597E-4	0.2270651079511112
>1k	2.1616389294522277E-4	0.13850161013132625
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.7482676415939444E-5	0.0
12-13	0.0	0.0	0.0	3.496535283187889E-5	0.0
14-15	0.0	0.0	0.0	3.496535283187889E-5	0.0
16-17	0.0	0.0	0.0	3.496535283187889E-5	0.0
18-19	0.0	0.0	0.0	3.496535283187889E-5	0.0
20-21	0.0	1.7482676415939444E-5	0.0	1.3986141132751555E-4	5.244802924781833E-5
22-23	0.0	3.496535283187889E-5	0.0	3.1468817548691003E-4	6.993070566375778E-5
24-25	0.0	3.496535283187889E-5	0.0	6.993070566375778E-4	6.993070566375778E-5
26-27	0.0	3.496535283187889E-5	0.0	0.0016608542595142474	6.993070566375778E-5
28-29	0.0	3.496535283187889E-5	0.0	0.008059513827748084	6.993070566375778E-5
30-31	1.7482676415939444E-5	3.496535283187889E-5	0.0	0.03160867896001852	6.993070566375778E-5
32-33	3.496535283187889E-5	3.496535283187889E-5	0.0	0.07818252893208119	6.993070566375778E-5
34-35	3.496535283187889E-5	3.496535283187889E-5	0.0	0.14164464432194138	6.993070566375778E-5
36-37	3.496535283187889E-5	3.496535283187889E-5	0.0	0.24645328943549835	6.993070566375778E-5
38-39	3.496535283187889E-5	3.496535283187889E-5	0.0	0.4148464286738271	6.993070566375778E-5
40-41	3.496535283187889E-5	3.496535283187889E-5	0.0	0.6133971847296513	6.993070566375778E-5
42-43	3.496535283187889E-5	3.496535283187889E-5	0.0	0.8222102718416322	6.993070566375778E-5
44-45	3.496535283187889E-5	3.496535283187889E-5	0.0	1.0649222685241195	6.993070566375778E-5
46-47	3.496535283187889E-5	3.496535283187889E-5	0.0	1.3590158511930528	6.993070566375778E-5
48-49	3.496535283187889E-5	3.496535283187889E-5	0.0	1.6674801938758885	6.993070566375778E-5
50-51	3.496535283187889E-5	3.496535283187889E-5	0.0	2.0081301438404684	6.993070566375778E-5
52-53	3.496535283187889E-5	3.496535283187889E-5	0.0	2.3450562837284536	6.993070566375778E-5
54-55	3.496535283187889E-5	3.496535283187889E-5	0.0	2.7281192066781026	6.993070566375778E-5
56-57	3.496535283187889E-5	6.993070566375778E-5	0.0	3.1762526512478786	6.993070566375778E-5
58-59	3.496535283187889E-5	6.993070566375778E-5	0.0	3.6577430424192667	6.993070566375778E-5
60-61	3.496535283187889E-5	6.993070566375778E-5	0.0	4.163988903395625	6.993070566375778E-5
62-63	3.496535283187889E-5	6.993070566375778E-5	0.0	4.682455155186725	6.993070566375778E-5
64-65	3.496535283187889E-5	6.993070566375778E-5	0.0	5.235222418105899	6.993070566375778E-5
66-67	3.496535283187889E-5	6.993070566375778E-5	0.0	5.863951910052329	6.993070566375778E-5
68-69	3.496535283187889E-5	6.993070566375778E-5	0.0	6.5553218315970705	6.993070566375778E-5
70-71	3.496535283187889E-5	6.993070566375778E-5	0.0	7.280555697359486	6.993070566375778E-5
72-73	3.496535283187889E-5	6.993070566375778E-5	0.0	8.01402390371381	6.993070566375778E-5
74-75	3.496535283187889E-5	6.993070566375778E-5	0.0	8.76334889757739	6.993070566375778E-5
76-77	3.496535283187889E-5	6.993070566375778E-5	0.0	9.577254898121453	6.993070566375778E-5
78-79	3.496535283187889E-5	6.993070566375778E-5	0.0	10.462909802676528	6.993070566375778E-5
80-81	3.496535283187889E-5	6.993070566375778E-5	0.0	11.385383223763572	6.993070566375778E-5
82-83	3.496535283187889E-5	6.993070566375778E-5	0.0	12.326388281851514	6.993070566375778E-5
84-85	3.496535283187889E-5	6.993070566375778E-5	0.0	13.270959805928307	6.993070566375778E-5
86	3.496535283187889E-5	6.993070566375778E-5	0.0	13.99404330249156	6.993070566375778E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5100	0.0	44.038265	1
GGTATCA	2900	0.0	37.61239	1
TCAACGC	6370	0.0	34.912785	4
ATCAACG	6545	0.0	34.259525	3
CAACGCA	6515	0.0	34.206135	5
TATCAAC	6590	0.0	33.818024	2
AACGCAG	6845	0.0	32.752857	6
GTACATG	10190	0.0	28.273577	1
ACGCAGA	8230	0.0	26.512613	7
TACATGG	10710	0.0	26.117868	2
CGCAGAG	8420	0.0	25.476583	8
ACATGGG	10940	0.0	24.771502	3
CATGGGG	7225	0.0	21.070868	4
GCAGAGT	9400	0.0	21.016697	9
GTATAGG	465	0.0	18.80534	1
GAGTACA	7595	0.0	18.663948	1
AGAGTAC	7715	0.0	18.388868	10-11
GTACGAA	230	2.7854567E-6	18.009233	1
TAAGGTA	565	0.0	17.854856	4
TACACCG	370	2.3464963E-10	17.35036	5
>>END_MODULE