FastQCFastQC Report
Mon 15 Aug 2016
SRR2920860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859974
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47250.043.0854031
GGTATCA28450.041.2955131
TCAACGC61850.032.2340354
TATCAAC63150.032.019232
ATCAACG63900.031.3484943
CAACGCA63450.031.2660985
AACGCAG66200.029.8961126
GTACATG101700.028.716771
TACATGG102250.027.8988172
ACATGGG108550.025.8004473
ACGCAGA83000.023.4445787
CGCAGAG86100.022.5425768
CATGGGG74950.021.3433864
GTATAGG5000.020.9286331
GAGTACA76100.020.31361
CTATACT5800.019.6421184
GCAGAGT94500.019.534459
AGTACAT77300.018.6053472
ATACCGT5300.017.9098016
AGAGTAC78850.017.06069810-11
Produced by FastQC (version 0.11.5)