##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920860_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2859974
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.225360789993196	31.0	30.0	34.0	26.0	34.0
2	30.228783898035438	31.0	30.0	34.0	26.0	34.0
3	30.371627504305984	31.0	30.0	34.0	26.0	34.0
4	34.01118122052858	35.0	35.0	37.0	30.0	37.0
5	33.52515827066959	35.0	35.0	37.0	28.0	37.0
6	33.47010742055697	35.0	35.0	37.0	28.0	37.0
7	33.285275670338265	35.0	33.0	37.0	27.0	37.0
8	33.20885224830715	35.0	33.0	37.0	27.0	37.0
9	34.54381788086185	38.0	34.0	39.0	27.0	39.0
10-11	34.34767344038792	37.5	34.0	39.0	27.0	39.0
12-13	34.161622098662434	37.0	34.0	39.0	26.0	39.0
14-15	35.051066897810955	38.0	34.0	40.0	26.0	41.0
16-17	34.91640570858337	38.0	34.0	40.0	25.0	41.0
18-19	34.81376281742422	38.0	33.5	40.0	25.0	41.0
20-21	34.691264326179194	38.0	34.0	40.0	24.5	41.0
22-23	34.53860437192786	38.0	33.5	40.0	24.0	41.0
24-25	34.35717230296499	38.0	33.0	40.0	23.0	41.0
26-27	34.15835843262911	38.0	33.0	40.0	22.5	41.0
28-29	34.09777046924203	38.0	33.0	40.0	22.5	41.0
30-31	34.08557105763899	38.0	33.0	40.0	22.0	41.0
32-33	33.93720572284923	38.0	33.0	40.0	21.0	41.0
34-35	33.76497653475172	38.0	33.0	40.0	19.5	41.0
36-37	33.562515428461936	38.0	32.0	40.0	18.5	41.0
38-39	33.36612081088849	38.0	32.0	40.0	17.5	41.0
40-41	33.124381025841494	38.0	32.0	40.0	15.5	41.0
42-43	33.15312132208195	38.0	32.0	40.0	15.5	41.0
44-45	33.007269471680516	38.0	32.0	40.0	15.0	41.0
46-47	32.744792260349215	37.0	31.0	40.0	12.5	41.0
48-49	32.466587108833856	37.0	31.0	40.0	9.0	41.0
50-51	32.18996151713267	37.0	31.0	40.0	8.0	41.0
52-53	31.91065583113693	36.5	30.0	40.0	7.5	41.0
54-55	31.610537543348297	36.0	30.0	39.0	6.0	41.0
56-57	31.260548872122612	36.0	29.5	39.0	2.0	40.5
58-59	30.905117668901887	35.0	29.0	39.0	2.0	40.0
60-61	30.552281419341575	35.0	29.0	39.0	2.0	40.0
62-63	30.212097207876717	35.0	28.0	38.0	2.0	40.0
64-65	29.78680015972173	34.0	28.0	38.0	2.0	40.0
66-67	29.33260879294707	34.0	28.0	37.0	2.0	40.0
68-69	28.87897459906978	34.0	26.5	37.0	2.0	39.0
70-71	28.445225201347984	34.0	26.0	36.0	2.0	39.0
72-73	28.004114722721255	33.0	26.0	36.0	2.0	39.0
74-75	27.567696594444563	33.0	26.0	35.0	2.0	37.5
76-77	26.147688580385697	31.5	23.5	34.0	2.0	36.0
78-79	26.99208699100062	32.5	25.0	35.0	2.0	36.0
80-81	27.02566124727008	33.0	25.0	35.0	2.0	36.0
82-83	26.699704088218986	33.0	25.0	35.0	2.0	36.0
84-85	26.3803842272692	33.0	24.5	35.0	2.0	35.5
86-87	25.845974823547348	32.0	23.0	35.0	2.0	35.0
88-89	25.485542700737838	32.0	20.5	35.0	2.0	35.0
90-91	25.11346396855356	32.0	19.5	34.0	2.0	35.0
92-93	24.80039731130423	32.0	18.0	34.0	2.0	35.0
94-95	24.403396499408736	31.5	13.5	34.0	2.0	35.0
96-97	23.858015667275296	31.0	2.0	34.0	2.0	35.0
98-99	23.237286947363856	31.0	2.0	34.0	2.0	35.0
100-101	21.031151157318213	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67647.0
3	31154.0
4	17481.0
5	12339.0
6	10581.0
7	10690.0
8	11962.0
9	14037.0
10	15719.0
11	17210.0
12	19046.0
13	21048.0
14	22321.0
15	23682.0
16	25259.0
17	25988.0
18	25424.0
19	24431.0
20	24565.0
21	25606.0
22	27508.0
23	31481.0
24	36278.0
25	42670.0
26	50699.0
27	59915.0
28	70397.0
29	83287.0
30	98164.0
31	116005.0
32	138767.0
33	170473.0
34	213354.0
35	276984.0
36	366347.0
37	408876.0
38	209740.0
39	12838.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.514879320643274	18.264473377673223	12.970276391547978	25.250370910135523
2	16.771026589752214	20.02168551182633	37.25373027866687	25.953557619754587
3	20.132514666345877	23.42240614797553	28.2710427443202	28.174036441358396
4	14.279139854586173	15.62130297531476	34.41623772583433	35.68331944426473
5	15.161536113307292	36.46882626287563	33.26555382386582	15.104083799951255
6	35.20590060665971	33.73687469611883	16.419157019186915	14.638067678034549
7	30.697363504924542	30.633003233070678	19.740044730038775	18.92958853196601
8	27.73048550405745	33.7866389669897	19.233875668641566	19.248999860311287
9	27.26297691747437	15.323967729284554	18.565991091937924	38.84706426130315
10-11	26.583174244165274	24.647411903516264	26.681139448926906	22.08827440339155
12-13	27.052069358131845	21.810967322423597	26.80590452536168	24.33105879408287
14-15	24.083071510462705	23.46154048117063	24.09773942571086	28.357648582655802
16-17	24.511621828579404	26.508626382979315	24.748552793197913	24.23119899524336
18-19	24.456796936485546	25.69651176340699	26.145222287116642	23.701469012990824
20-21	25.29076234476351	24.857635760218045	25.797764426709353	24.05383746830909
22-23	24.849808102226028	24.71623031372408	25.796856075094727	24.63710550895517
24-25	24.621326024084624	24.913790434267405	25.82095664323138	24.64392689841659
26-27	24.447450382630233	25.147876355405696	25.81896410712702	24.585709154837055
28-29	24.494753085717992	25.27052229179941	25.734742025038344	24.499982597444255
30-31	24.693474201539512	25.160751827158517	25.583331963496352	24.562442007805625
32-33	24.55986880752765	25.129787263438285	25.525571741642327	24.784772187391734
34-35	24.713336902887946	25.11260359373246	25.65121930832943	24.522840195050165
36-37	24.877218783580673	24.94727205799418	25.53753122538146	24.63797793304369
38-39	24.76900775072057	24.996427977444753	25.5943417064107	24.640222565423976
40-41	24.751509983756055	24.985612332316563	25.642469911806355	24.620407772121027
42-43	24.794456762788215	24.857718321005372	25.536564848430167	24.811260067776246
44-45	24.525186128996044	24.890081852339822	25.73305287927663	24.85167913938751
46-47	24.666392208945105	24.86441800214133	25.622435484655142	24.84675430425842
48-49	24.514208792874616	24.87882867044528	25.60735156479346	24.999610971886643
50-51	24.367558229301178	25.163888347320373	25.60679910858308	24.86175431479537
52-53	24.600409787698222	24.905136717995326	25.67782028891449	24.816633205391966
54-55	24.27646818558224	24.901821383809672	25.764936438307114	25.05677399230098
56-57	24.20559296679565	25.00750050877577	25.598204913200405	25.188701611228176
58-59	24.17871086527835	24.74578635896272	25.76097999596832	25.31452277979061
60-61	24.113959976455504	24.77463629664526	25.874481117013552	25.23692260988568
62-63	24.000797298974877	24.86945103517732	25.752791857759068	25.37695980808874
64-65	23.927935381515585	24.796266372560574	25.69260691272875	25.583191333195092
66-67	23.898892898188226	24.905409510836915	25.582291408142066	25.613406182832794
68-69	24.093519748941585	24.661302475761573	25.567616488464985	25.677561286831857
70-71	23.82211335431613	24.605549593584364	25.64761317044416	25.92472388165535
72-73	23.87236012693464	24.620461538219356	25.51071113798484	25.99646719686116
74-75	23.781262319926743	24.733487120432518	25.499949517281976	25.98530104235876
76-77	23.790594893699808	24.452697971449382	25.696522797641848	26.060184337208963
78-79	23.72856178281562	24.519208814066197	25.592797182449484	26.159432220668698
80-81	23.669597877917294	24.502326492286116	25.594247854923402	26.233827774873188
82-83	23.624231905688692	24.457776953262762	25.64768384037801	26.27030730067053
84-85	23.61243755606185	24.39495167971352	25.494542002017788	26.498068762206838
86-87	23.54136416377903	24.31158187470188	25.627019132820088	26.520034828699004
88-89	23.404101470787715	24.246577342807242	25.52399638210044	26.8253248043046
90-91	23.32309876808323	24.213216700777014	25.605567510003024	26.858117021136728
92-93	23.428630880040394	24.10531241233133	25.48960678292136	26.97644992470692
94-95	23.245055041499224	23.96384810413391	25.656492652100592	27.134604202266278
96-97	23.29948766436211	23.7526417524872	25.711699659142244	27.23617092400844
98-99	23.163602770199724	23.800328406995295	25.62525656796507	27.41081225483991
100-101	23.274128630665494	23.627000057870703	25.691725380954455	27.407145930509348
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	44.0
2	51.5
3	85.5
4	77.5
5	74.0
6	134.5
7	289.5
8	423.0
9	436.0
10	397.0
11	370.5
12	351.0
13	377.5
14	443.5
15	503.0
16	564.0
17	605.5
18	693.5
19	793.0
20	870.0
21	976.0
22	1170.5
23	1446.0
24	1711.0
25	2063.5
26	2601.5
27	3288.5
28	4171.0
29	5495.5
30	7539.5
31	10911.5
32	15444.0
33	20036.0
34	24879.5
35	30626.0
36	37260.5
37	43521.0
38	49838.5
39	56207.0
40	61752.5
41	67264.0
42	73047.0
43	80552.5
44	88445.5
45	95547.0
46	103731.0
47	112074.0
48	119373.5
49	125050.0
50	128035.0
51	130831.5
52	134752.0
53	138323.5
54	138058.0
55	133347.5
56	125356.0
57	114839.0
58	105330.5
59	94213.0
60	80128.0
61	66465.0
62	54265.5
63	41980.0
64	31114.5
65	23401.0
66	17325.5
67	12735.0
68	9398.0
69	6818.0
70	5082.0
71	3831.0
72	2859.0
73	2386.0
74	1878.5
75	1344.0
76	953.0
77	609.0
78	325.0
79	154.0
80	88.0
81	52.0
82	27.0
83	12.0
84	6.5
85	5.0
86	3.0
87	2.0
88	0.5
89	0.5
90	1.0
91	0.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19262412875082083
2	0.0
3	0.012797319136467674
4	0.014370760013902225
5	0.007377689447526446
6	0.11129471806387052
7	0.14598034807309437
8	0.12667947330989723
9	0.14454676860698734
10-11	0.11704651860471459
12-13	0.11718638001604209
14-15	0.11884723427555637
16-17	0.0688292970495536
18-19	0.0818538909794285
20-21	0.038811541643385565
22-23	0.0861196640249177
24-25	0.058654379375476844
26-27	0.06739571758344656
28-29	0.04190597536900685
30-31	0.026328910682404803
32-33	0.033863944217674705
34-35	0.021223969168950488
36-37	0.025227502068200618
38-39	0.033199602513869005
40-41	0.026381358711652623
42-43	0.014598034807309436
44-45	0.028007247618334993
46-47	0.03507024889037453
48-49	0.009912677527837666
50-51	0.016835817388549685
52-53	0.007010553242791718
54-55	0.013636487604432767
56-57	0.005874179275755654
58-59	0.0043881517804008
60-61	0.024720504452138376
62-63	0.011153947553369365
64-65	0.00821685791549154
66-67	0.04208080213316624
68-69	0.09199384330067335
70-71	0.02264006595864158
72-73	0.046766159412638016
74-75	0.0895287859260259
76-77	0.2559988307586013
78-79	0.0809273091293837
80-81	0.018147018119745145
82-83	0.013304316752529917
84-85	0.014300829308238467
86-87	0.270229729361176
88-89	0.6291490761804128
90-91	0.26963531836303406
92-93	0.35351719980671154
94-95	0.0014335794661070346
96-97	0.004545495868144255
98-99	0.0037762581058429206
100-101	0.005262285601197773
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2859974.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.09340167177034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.83218427532016	32.188029194194165
2	12.624716917866023	11.638322957751859
3	5.826257901578886	8.056561371024042
4	3.3457824784520063	6.168739827426385
5	2.049146548783356	4.722606747869658
6	1.3966305985014953	3.8625273098288657
7	0.9961960201891839	3.2142644311679356
8	0.7397197957490311	2.7276961336016003
9	0.5453305736349549	2.2622527057007615
>10	2.518444367919358	19.281881886203134
>50	0.09181582677825972	2.8653704171485517
>100	0.032631290010597724	2.592684431204097
>500	8.38497158987167E-4	0.255963020501508
>1k	3.0490805777850757E-4	0.16309956637757236
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.7482676415939444E-5	0.0
12-13	0.0	0.0	0.0	3.496535283187889E-5	0.0
14-15	0.0	0.0	0.0	3.496535283187889E-5	0.0
16-17	0.0	0.0	0.0	5.244802924781833E-5	0.0
18-19	0.0	0.0	0.0	6.993070566375778E-5	0.0
20-21	0.0	0.0	0.0	1.3986141132751555E-4	0.0
22-23	0.0	0.0	0.0	3.321708519028495E-4	0.0
24-25	0.0	0.0	0.0	7.517550858853962E-4	1.7482676415939444E-5
26-27	0.0	0.0	0.0	0.001765750318009884	3.496535283187889E-5
28-29	0.0	0.0	0.0	0.008321753973987177	3.496535283187889E-5
30-31	0.0	0.0	0.0	0.03181847107700979	3.496535283187889E-5
32-33	0.0	0.0	0.0	0.07844476907832029	3.496535283187889E-5
34-35	0.0	0.0	0.0	0.14103275064738352	3.496535283187889E-5
36-37	1.7482676415939444E-5	0.0	0.0	0.24526446743921448	3.496535283187889E-5
38-39	3.496535283187889E-5	0.0	0.0	0.41301074765015344	3.496535283187889E-5
40-41	3.496535283187889E-5	0.0	0.0	0.6113517115889865	3.496535283187889E-5
42-43	3.496535283187889E-5	0.0	0.0	0.8203221427887106	3.496535283187889E-5
44-45	3.496535283187889E-5	0.0	0.0	1.0623348324145603	3.496535283187889E-5
46-47	3.496535283187889E-5	0.0	0.0	1.3548549742060592	3.496535283187889E-5
48-49	3.496535283187889E-5	0.0	0.0	1.661256361071814	3.496535283187889E-5
50-51	3.496535283187889E-5	0.0	0.0	1.996976196287099	3.496535283187889E-5
52-53	3.496535283187889E-5	0.0	0.0	2.3299337686286656	3.496535283187889E-5
54-55	3.496535283187889E-5	0.0	0.0	2.7058462769241958	3.496535283187889E-5
56-57	3.496535283187889E-5	0.0	0.0	3.147878267424809	3.496535283187889E-5
58-59	3.496535283187889E-5	0.0	0.0	3.622270691971326	3.496535283187889E-5
60-61	3.496535283187889E-5	0.0	0.0	4.12164586111622	3.496535283187889E-5
62-63	3.496535283187889E-5	0.0	0.0	4.634325347013645	3.496535283187889E-5
64-65	3.496535283187889E-5	0.0	0.0	5.179767368514539	3.496535283187889E-5
66-67	3.496535283187889E-5	0.0	0.0	5.798601665609548	6.993070566375778E-5
68-69	3.496535283187889E-5	0.0	0.0	6.477156785341405	6.993070566375778E-5
70-71	3.496535283187889E-5	0.0	0.0	7.1911143248155405	6.993070566375778E-5
72-73	3.496535283187889E-5	0.0	0.0	7.9135334796749905	6.993070566375778E-5
74-75	3.496535283187889E-5	0.0	0.0	8.66006124531202	6.993070566375778E-5
76-77	3.496535283187889E-5	0.0	0.0	9.466799348525544	6.993070566375778E-5
78-79	3.496535283187889E-5	0.0	0.0	10.344394739252873	6.993070566375778E-5
80-81	3.496535283187889E-5	0.0	0.0	11.259175782716905	6.993070566375778E-5
82-83	3.496535283187889E-5	0.0	0.0	12.193554906443206	6.993070566375778E-5
84-85	3.496535283187889E-5	0.0	0.0	13.130503983602647	6.993070566375778E-5
86-87	3.496535283187889E-5	0.0	0.0	14.095495273733258	6.993070566375778E-5
88-89	3.496535283187889E-5	0.0	0.0	15.12957810105966	6.993070566375778E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4725	0.0	43.085403	1
GGTATCA	2845	0.0	41.295513	1
TCAACGC	6185	0.0	32.234035	4
TATCAAC	6315	0.0	32.01923	2
ATCAACG	6390	0.0	31.348494	3
CAACGCA	6345	0.0	31.266098	5
AACGCAG	6620	0.0	29.896112	6
GTACATG	10170	0.0	28.71677	1
TACATGG	10225	0.0	27.898817	2
ACATGGG	10855	0.0	25.800447	3
ACGCAGA	8300	0.0	23.444578	7
CGCAGAG	8610	0.0	22.542576	8
CATGGGG	7495	0.0	21.343386	4
GTATAGG	500	0.0	20.928633	1
GAGTACA	7610	0.0	20.3136	1
CTATACT	580	0.0	19.642118	4
GCAGAGT	9450	0.0	19.53445	9
AGTACAT	7730	0.0	18.605347	2
ATACCGT	530	0.0	17.909801	6
AGAGTAC	7885	0.0	17.060698	10-11
>>END_MODULE