FastQCFastQC Report
Mon 15 Aug 2016
SRR2920859_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920859_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4452935
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73700.041.3432851
GGTATCA41800.041.072111
ATCAACG97150.030.9164753
TCAACGC96850.030.8702014
TATCAAC98950.030.5871582
CAACGCA99000.030.1997915
AACGCAG103750.028.9000236
GTACATG169550.028.1239571
TACATGG174300.026.940992
ACATGGG184200.025.1931443
ACGCAGA125500.023.7423957
CGCAGAG131500.022.5178818
CATGGGG135050.021.9685384
GCAGAGT147550.018.9186049
AGTACAT120700.018.8065412
GAGTACA128750.018.7326181
AGAGTAC126050.016.96443210-11
ATGGGGG70650.016.161395
GTATATA13200.016.0397781
CAGAGTA134600.015.93790810-11
Produced by FastQC (version 0.11.5)