##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920859_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4452935
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.396909229530635	31.0	28.0	33.0	16.0	34.0
2	28.429280238763873	31.0	28.0	34.0	16.0	34.0
3	28.497359157499492	31.0	28.0	34.0	16.0	34.0
4	31.945866714874573	35.0	32.0	37.0	19.0	37.0
5	31.897305934176	35.0	32.0	37.0	20.0	37.0
6	32.02231561879974	35.0	32.0	37.0	22.0	37.0
7	31.855233009239974	35.0	32.0	37.0	19.0	37.0
8	31.791156170031677	35.0	32.0	37.0	18.0	37.0
9	33.029953951719484	37.0	32.0	39.0	18.0	39.0
10-11	32.83891051632238	37.0	32.0	39.0	17.0	39.0
12-13	32.658671864736405	37.0	32.0	39.0	17.0	39.0
14-15	33.40372832749636	38.0	32.0	40.0	17.0	41.0
16-17	33.28425981964705	38.0	32.0	40.0	16.0	41.0
18-19	33.158564182949	38.0	32.0	40.0	13.0	41.0
20-21	33.05462475872655	38.0	32.0	40.0	10.5	41.0
22-23	32.828899254087474	38.0	32.0	40.0	10.0	41.0
24-25	32.629325040675425	38.0	31.0	40.0	10.0	41.0
26-27	32.65061178750644	38.0	31.0	40.0	9.5	41.0
28-29	32.485505627187464	38.0	31.0	40.0	8.5	41.0
30-31	32.236675136735656	37.5	30.5	40.0	8.0	41.0
32-33	31.996612908115658	37.0	30.0	40.0	5.0	41.0
34-35	31.66452036690408	37.0	30.0	40.0	2.0	41.0
36-37	31.36035378913009	37.0	30.0	40.0	2.0	41.0
38-39	31.160420935854667	37.0	30.0	40.0	2.0	41.0
40-41	30.95118208552337	36.5	29.5	40.0	2.0	41.0
42-43	30.70731955440625	36.0	29.0	39.5	2.0	41.0
44-45	30.421210617267036	36.0	28.0	39.0	2.0	40.5
46-47	30.156795349584037	35.5	28.0	39.0	2.0	40.0
48-49	29.847275897806725	35.0	27.0	39.0	2.0	40.0
50-51	28.697304586750086	34.0	25.5	38.0	2.0	39.5
52-53	29.142864874515347	34.5	26.5	38.5	2.0	39.5
54-55	29.77555982739474	35.0	28.0	39.0	2.0	40.0
56-57	29.708741650170055	35.0	28.0	39.0	2.0	40.0
58-59	29.446983281813004	35.0	27.0	39.0	2.0	40.0
60-61	29.103342402258285	35.0	26.0	39.0	2.0	40.0
62-63	28.69723137660891	34.0	26.0	38.0	2.0	40.0
64-65	28.262876282721393	34.0	26.0	38.0	2.0	40.0
66-67	27.866277073435835	34.0	25.0	37.0	2.0	40.0
68-69	27.413845138094313	33.5	23.5	36.5	2.0	39.0
70-71	27.076384227481427	33.0	23.0	36.0	2.0	39.0
72-73	26.619669723452063	33.0	22.0	35.5	2.0	39.0
74-75	26.179260532659917	33.0	20.5	35.0	2.0	37.5
76-77	25.769495400224795	32.0	19.5	35.0	2.0	37.0
78-79	25.314705806395107	32.0	18.0	35.0	2.0	36.5
80-81	24.87316320584064	32.0	15.0	35.0	2.0	36.0
82-83	24.409973422023903	31.0	8.5	34.5	2.0	35.5
84-85	24.01554727387667	31.0	4.5	34.0	2.0	35.0
86-87	23.558561488097176	31.0	2.0	34.0	2.0	35.0
88-89	23.028976169649905	30.5	2.0	34.0	2.0	35.0
90-91	22.52950548346203	30.0	2.0	34.0	2.0	35.0
92-93	21.988498035565307	30.0	2.0	34.0	2.0	35.0
94-95	21.34775490771817	29.5	2.0	34.0	2.0	35.0
96-97	20.696670847429843	29.0	2.0	34.0	2.0	35.0
98	20.2953130912533	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	255540.0
3	42361.0
4	30992.0
5	25150.0
6	23626.0
7	23937.0
8	26912.0
9	31274.0
10	33608.0
11	35768.0
12	36769.0
13	36670.0
14	33644.0
15	35364.0
16	37480.0
17	40335.0
18	41223.0
19	41904.0
20	43082.0
21	45701.0
22	49707.0
23	55551.0
24	63285.0
25	72898.0
26	83736.0
27	96092.0
28	111559.0
29	129288.0
30	150326.0
31	176793.0
32	209918.0
33	254213.0
34	316517.0
35	400360.0
36	505890.0
37	536856.0
38	292705.0
39	25900.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.83911287658469	17.175709795469494	12.892617369454888	25.092559958490927
2	16.3227705117368	18.950428411007703	38.825698056218286	25.901103021037216
3	19.992816677706703	22.78438420307014	27.881128819340795	29.341670299882367
4	13.793734692520696	14.807442467237728	34.952377230197534	36.446445610044044
5	15.26237571274797	36.204159950258145	33.11941909131261	15.414045245681281
6	35.09667695431655	33.27419057759089	16.762018356042535	14.867114112050029
7	30.908179402812696	30.52117719647336	19.739111548051504	18.831531852662444
8	27.67241708460324	33.72035046031044	19.251554768968855	19.355677686117467
9	27.493911319163654	15.71543263038872	18.606379837118663	38.18427621332896
10-11	26.661595105250807	24.70884933195746	26.428142337581846	22.20141322520989
12-13	27.02275660129639	21.80177313199269	26.720332401611074	24.45513786509985
14-15	24.160062163552126	23.408922985018314	24.10861104436115	28.322403807068408
16-17	24.553314308731046	26.457043442952187	24.705021531533262	24.2846207167835
18-19	24.52050866121195	25.714408312175436	26.04248443724897	23.722598589363646
20-21	25.29142051748332	24.931857005740362	25.771194395681913	24.005528081094404
22-23	25.022117460660372	24.606800387149697	25.66493162667053	24.70615052551941
24-25	24.719066152756596	24.860798544158673	25.717889618849533	24.702245684235198
26-27	24.485242062537786	25.075503374559215	25.742695197405535	24.696559365497464
28-29	24.53518358457791	25.208793112610817	25.693268410055687	24.56275489275559
30-31	24.75359493557686	25.108903567399327	25.460077829509824	24.677423667513988
32-33	24.553968976139835	25.08419605250316	25.390825008442906	24.971009962914092
34-35	24.866134615904727	25.115316480801997	25.494070210866997	24.52447869242628
36-37	24.920351987928797	24.928605550933234	25.506433996730866	24.644608464407103
38-39	24.83684434867916	25.049246604537608	25.41638279217837	24.69752625460486
40-41	24.803457225134405	24.951262854411567	25.543365704376324	24.7019142160777
42-43	24.868304701729986	24.782055124602213	25.39784958123382	24.951790592433973
44-45	24.68123103586014	24.914569037983426	25.556178060882374	24.84802186527406
46-47	24.72123518213469	24.925792967312592	25.47854669356513	24.874425156987584
48-49	24.719078825038935	24.950303960810125	25.412156605697096	24.918460608453845
50-51	24.51445267701489	25.101257760012935	25.561449064198	24.822840498774173
52-53	24.6258526734222	24.920161030730263	25.617283149687246	24.83670314616029
54-55	24.4204878453864	24.85441678028544	25.742944917348265	24.982150456979895
56-57	24.405512657424012	24.86945766078866	25.718042082604587	25.00698759918274
58-59	24.392768733682566	24.741317574616133	25.712452281198367	25.15346141050293
60-61	24.399465221279755	24.693056618168647	25.95342759975695	24.954050560794645
62-63	24.218696522607065	24.75666323734735	25.993642734393298	25.030997505652287
64-65	24.22895893222962	24.634129582624926	25.925821874972847	25.211089610172603
66-67	24.211626931977136	24.814917845653994	25.75015531428784	25.223299908081025
68-69	24.290048584067172	24.645307662437222	25.77636441580527	25.28827933769034
70-71	24.163469533895018	24.57675192955305	25.87439777562531	25.38538076092662
72-73	24.225093783628505	24.63859727352582	25.78530438917864	25.35100455366703
74-75	24.124361938287624	24.786185793473	25.800007167523276	25.2894451007161
76-77	24.15451761815353	24.52779327630501	25.87692958542319	25.440759520118267
78-79	24.16758548628622	24.66344381106244	25.784418772589213	25.384551930062127
80-81	24.183301136857395	24.744059713557878	25.79417316485441	25.278465984730314
82-83	24.22084150976613	24.699294554791614	25.818352395302686	25.261511540139576
84-85	24.17211377594562	24.666354694837903	25.79763577649653	25.36389575271995
86-87	24.132386693446286	24.661138778349528	25.914813453771746	25.291661074432444
88-89	24.145269032385354	24.53341976944108	25.8428478818456	25.47846331632797
90-91	24.169403694765318	24.54313638566211	25.871021925643685	25.416437993928888
92-93	24.2605845717596	24.429437315306746	25.88971713615288	25.420260976780774
94-95	24.186872306786615	24.383748593479154	25.994263570312633	25.435115529421605
96-97	24.377184208752592	24.274346199806452	25.89013375027586	25.4583358411651
98	24.38935489368148	24.23255727981837	25.77977283731549	25.59831498918466
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	65.5
2	100.0
3	165.5
4	172.5
5	202.0
6	346.5
7	651.5
8	1017.0
9	1155.5
10	1192.5
11	1329.5
12	1529.0
13	1754.0
14	2019.5
15	2272.0
16	2467.0
17	2581.0
18	2607.0
19	2582.0
20	2547.5
21	2514.5
22	2566.0
23	2781.0
24	3082.5
25	3607.0
26	4411.5
27	5499.5
28	7109.0
29	9736.0
30	14199.0
31	20782.0
32	28318.5
33	35397.0
34	43162.0
35	53181.5
36	64217.5
37	75046.5
38	85920.5
39	96776.0
40	106706.5
41	116993.5
42	130809.0
43	144988.0
44	156852.5
45	170943.0
46	184727.5
47	196304.0
48	206401.0
49	211876.5
50	213337.0
51	213347.0
52	212655.5
53	212986.0
54	211162.5
55	202675.5
56	188500.0
57	170935.0
58	154800.5
59	138456.5
60	118771.0
61	98253.0
62	79614.5
63	61965.0
64	45777.5
65	33881.5
66	25471.0
67	19034.5
68	13888.0
69	10093.0
70	7553.5
71	5880.5
72	4608.5
73	3492.5
74	2634.5
75	1822.0
76	1174.5
77	701.5
78	386.5
79	233.0
80	148.0
81	95.0
82	60.0
83	40.0
84	29.0
85	22.0
86	17.0
87	14.5
88	12.0
89	7.0
90	5.0
91	6.0
92	6.0
93	5.5
94	4.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4096623912093933
2	0.028116287347558406
3	0.021468986185515845
4	0.01938047602311734
5	0.025848120396996587
6	0.022344813027811994
7	0.017157223269596344
8	0.011340834752809103
9	0.0
10-11	0.0
12-13	0.005591817531583102
14-15	0.003705421255868321
16-17	0.006276759036455731
18-19	0.03776161116207625
20-21	0.08200209524729195
22-23	0.10165205645265427
24-25	0.067360067011982
26-27	0.07781384637323473
28-29	0.05965728221947996
30-31	0.05475040619276948
32-33	0.021884442508143503
34-35	0.3596504328044312
36-37	0.551198254634303
38-39	0.503634119968066
40-41	0.5118534180265376
42-43	0.4592364361932074
44-45	0.4320296613357258
46-47	0.43172649050570017
48-49	0.4205877696395748
50-51	0.4173314903541147
52-53	0.4796499387482638
54-55	0.48168230616436125
56-57	0.4509834524869552
58-59	0.435892282281237
60-61	0.4507925671495317
62-63	0.42916638127437295
64-65	0.4793579964674984
66-67	0.503095149603576
68-69	0.4422139555147335
70-71	0.3977376718950535
72-73	0.3831068722090037
74-75	0.36506259354785103
76-77	0.3623115989791003
78-79	0.3611775155038194
80-81	0.37931162255905376
82-83	0.37410157570231767
84-85	0.3736187480841288
86-87	0.3588307487084361
88-89	0.40557519927867797
90-91	0.3820064743815034
92-93	0.3711035530498424
94-95	0.3795361935442579
96-97	0.37799788229560954
98	0.37375349067525127
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4452935.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.81318828657635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.47394297418474	34.65210281295292
2	11.700451979406974	11.188718270588613
3	5.405773261374348	7.754017643418947
4	2.983190780506274	5.705434499325005
5	1.8697189709323505	4.469861260008613
6	1.1947193169922452	3.427400379177607
7	0.8431247328370652	2.821873712014559
8	0.6277100823559988	2.401025628565577
9	0.4703258068585986	2.0238998723429504
>10	2.2924301184573266	18.85602518456874
>50	0.10101139593011914	3.289568130212084
>100	0.03641740615514873	2.9456447653992734
>500	9.465393249039484E-4	0.28437272852787887
>1k	2.3663468390251708E-4	0.18005511289712095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.2457098520414065E-5	0.0
6	4.491419704082813E-5	0.0	0.0	2.2457098520414065E-5	0.0
7	8.982839408165626E-5	0.0	0.0	4.491419704082813E-5	0.0
8	8.982839408165626E-5	0.0	0.0	4.491419704082813E-5	0.0
9	8.982839408165626E-5	0.0	0.0	4.491419704082813E-5	0.0
10-11	8.982839408165626E-5	0.0	0.0	7.859984482144923E-5	0.0
12-13	1.1228549260207031E-4	0.0	0.0	8.982839408165626E-5	0.0
14-15	1.796567881633125E-4	0.0	0.0	8.982839408165626E-5	0.0
16-17	2.2457098520414063E-4	0.0	0.0	8.982839408165626E-5	0.0
18-19	3.8177067484703906E-4	0.0	0.0	8.982839408165626E-5	0.0
20-21	5.165132659695234E-4	0.0	0.0	1.0105694334186329E-4	0.0
22-23	5.614274630103516E-4	0.0	0.0	2.357995344643477E-4	0.0
24-25	6.737129556124219E-4	0.0	0.0	6.063416600511798E-4	0.0
26-27	6.737129556124219E-4	0.0	0.0	0.0012800546156636018	0.0
28-29	8.758268422961485E-4	0.0	0.0	0.0054570749404606175	0.0
30-31	9.881123348982187E-4	0.0	0.0	0.021019844215107567	2.2457098520414065E-5
32-33	0.0010105694334186328	0.0	0.0	0.054155293081978514	2.2457098520414065E-5
34-35	0.0011116263767604962	0.0	0.0	0.100282173442909	2.2457098520414065E-5
36-37	0.0012126833201023595	0.0	0.0	0.17900553230622052	2.2457098520414065E-5
38-39	0.0013025117141840156	0.0	0.0	0.3064271093110499	2.2457098520414065E-5
40-41	0.0013137402634442227	0.0	0.0	0.4576644392967784	2.2457098520414065E-5
42-43	0.001414797206786086	0.0	0.0	0.6178958372399328	2.2457098520414065E-5
44-45	0.0016393681919902267	0.0	0.0	0.8130255662838106	2.2457098520414065E-5
46-47	0.0019986817683168515	0.0	0.0	1.0466692192901985	2.2457098520414065E-5
48-49	0.002110967260918922	0.0	0.0	1.296133898204218	2.2457098520414065E-5
50-51	0.002110967260918922	0.0	0.0	1.5661243651658963	2.2457098520414065E-5
52-53	0.002110967260918922	0.0	0.0	1.8487244929467868	2.2457098520414065E-5
54-55	0.002110967260918922	0.0	0.0	2.1663576944195233	2.2457098520414065E-5
56-57	0.0022457098520414064	0.0	0.0	2.533789511861278	2.2457098520414065E-5
58-59	0.0022457098520414064	0.0	0.0	2.9346711775491894	2.2457098520414065E-5
60-61	0.0022457098520414064	0.0	0.0	3.3621757335330518	2.2457098520414065E-5
62-63	0.0022457098520414064	0.0	0.0	3.8009425244249018	2.2457098520414065E-5
64-65	0.0022457098520414064	0.0	0.0	4.2655237500659675	2.2457098520414065E-5
66-67	0.002380452443163891	0.0	0.0	4.791525140160366	2.2457098520414065E-5
68-69	0.002414138090944512	0.0	0.0	5.377801382683556	4.491419704082813E-5
70-71	0.00245905228798534	0.0	0.0	6.005623257469511	4.491419704082813E-5
72-73	0.0024927379357659613	0.0	0.0	6.641933915496184	6.737129556124219E-5
74-75	0.002515195034286375	0.0	0.0	7.297557678250412	6.737129556124219E-5
76-77	0.002515195034286375	0.0	0.0	8.008908730983048	6.737129556124219E-5
78-79	0.002515195034286375	0.0	0.0	8.787530022333584	6.737129556124219E-5
80-81	0.0028969657091334144	0.0	0.0	9.602105128415303	6.737129556124219E-5
82-83	0.003020479750995692	0.0	0.0	10.439406818199682	6.737129556124219E-5
84-85	0.0031103081450773477	0.0	0.0	11.279246160116866	6.737129556124219E-5
86	0.003166450891378383	0.0	0.0	11.931433986797472	6.737129556124219E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7370	0.0	41.343285	1
GGTATCA	4180	0.0	41.07211	1
ATCAACG	9715	0.0	30.916475	3
TCAACGC	9685	0.0	30.870201	4
TATCAAC	9895	0.0	30.587158	2
CAACGCA	9900	0.0	30.199791	5
AACGCAG	10375	0.0	28.900023	6
GTACATG	16955	0.0	28.123957	1
TACATGG	17430	0.0	26.94099	2
ACATGGG	18420	0.0	25.193144	3
ACGCAGA	12550	0.0	23.742395	7
CGCAGAG	13150	0.0	22.517881	8
CATGGGG	13505	0.0	21.968538	4
GCAGAGT	14755	0.0	18.918604	9
AGTACAT	12070	0.0	18.806541	2
GAGTACA	12875	0.0	18.732618	1
AGAGTAC	12605	0.0	16.964432	10-11
ATGGGGG	7065	0.0	16.16139	5
GTATATA	1320	0.0	16.039778	1
CAGAGTA	13460	0.0	15.937908	10-11
>>END_MODULE