FastQCFastQC Report
Mon 15 Aug 2016
SRR2920859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4452935
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80500.044.0665051
GGTATCA45200.041.9759141
TCAACGC105700.032.871874
ATCAACG106450.032.774043
TATCAAC108500.032.283512
CAACGCA107950.031.9625515
AACGCAG113300.030.6630766
GTACATG167150.025.9165781
ACGCAGA135950.025.0307877
TACATGG173700.024.7561112
CGCAGAG141900.023.8458258
ACATGGG182050.022.9185143
GCAGAGT161900.020.1379439
CATGGGG132000.019.6339194
AGAGTAC137600.017.8126210-11
CAGAGTA147800.016.45490510-11
TACACTG37800.016.4478515
GAGTACA132800.016.1847521
AGTACAT126150.016.1031422
GTATTAA17050.015.618141
Produced by FastQC (version 0.11.5)