##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920859_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4452935 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.205866692417473 31.0 30.0 34.0 26.0 34.0 2 30.215545701879773 31.0 30.0 34.0 26.0 34.0 3 30.35857675892417 31.0 30.0 34.0 26.0 34.0 4 33.991013792027054 35.0 35.0 37.0 30.0 37.0 5 33.49035164447718 35.0 35.0 37.0 28.0 37.0 6 33.4300190773052 35.0 35.0 37.0 28.0 37.0 7 33.235681634697116 35.0 33.0 37.0 27.0 37.0 8 33.16026777844276 35.0 33.0 37.0 27.0 37.0 9 34.49250168708953 38.0 34.0 39.0 27.0 39.0 10-11 34.294503625137125 37.5 34.0 39.0 27.0 39.0 12-13 34.10499120243165 37.0 34.0 39.0 26.0 39.0 14-15 34.99865919893284 38.0 34.0 40.0 25.0 41.0 16-17 34.8605616295769 38.0 34.0 40.0 25.0 41.0 18-19 34.756937615303166 38.0 33.5 40.0 25.0 41.0 20-21 34.63417274224753 38.0 34.0 40.0 24.0 41.0 22-23 34.48043964261774 38.0 33.0 40.0 23.5 41.0 24-25 34.293280386980726 38.0 33.0 40.0 23.0 41.0 26-27 34.09675741954464 38.0 33.0 40.0 22.0 41.0 28-29 34.03679168907698 38.0 33.0 40.0 21.5 41.0 30-31 34.03085571651057 38.0 33.0 40.0 21.5 41.0 32-33 33.887365411801426 38.0 33.0 40.0 19.5 41.0 34-35 33.71385412093372 38.0 33.0 40.0 18.5 41.0 36-37 33.512553854929386 38.0 32.0 40.0 18.0 41.0 38-39 33.328461902093785 38.0 32.0 40.0 16.5 41.0 40-41 33.084338307206366 38.0 32.0 40.0 15.5 41.0 42-43 33.10488318378778 38.0 32.0 40.0 15.0 41.0 44-45 32.952309207298114 38.0 32.0 40.0 14.5 41.0 46-47 32.69232809371796 37.0 31.0 40.0 10.0 41.0 48-49 32.412358365886774 37.0 31.0 40.0 8.5 41.0 50-51 32.137573869818446 37.0 31.0 40.0 8.0 41.0 52-53 31.85401819249551 36.5 30.0 40.0 6.5 41.0 54-55 31.549618734609872 36.0 30.0 39.5 2.0 41.0 56-57 31.197971562576143 36.0 29.5 39.0 2.0 40.5 58-59 30.836667613607652 35.0 29.0 39.0 2.0 40.0 60-61 30.48509724485087 35.0 28.5 39.0 2.0 40.0 62-63 30.141962211440323 35.0 28.0 38.0 2.0 40.0 64-65 29.716604778645994 34.0 28.0 38.0 2.0 40.0 66-67 29.270249285022125 34.0 27.5 37.0 2.0 40.0 68-69 28.818649722037264 34.0 26.0 37.0 2.0 39.0 70-71 28.382234863073457 34.0 26.0 36.0 2.0 39.0 72-73 27.940291627881386 33.0 26.0 36.0 2.0 39.0 74-75 27.505093943657386 33.0 25.5 35.0 2.0 37.5 76-77 26.100020548245148 31.5 23.5 34.0 2.0 36.0 78-79 26.943130654276338 32.5 25.0 35.0 2.0 36.0 80-81 26.975333347556163 33.0 25.0 35.0 2.0 36.0 82-83 26.656257502074475 33.0 25.0 35.0 2.0 36.0 84-85 26.35031602751893 33.0 24.0 35.0 2.0 35.5 86-87 25.83053424314525 32.0 23.0 35.0 2.0 35.0 88-89 25.473607182678393 32.0 20.5 35.0 2.0 35.0 90-91 25.111503873288065 32.0 19.5 34.0 2.0 35.0 92-93 24.807030980690264 32.0 18.0 34.0 2.0 35.0 94-95 24.427724074121898 32.0 13.0 34.0 2.0 35.0 96-97 23.89639496197452 31.0 2.0 34.0 2.0 35.0 98-99 23.28650586635556 31.0 2.0 34.0 2.0 35.0 100-101 21.078820934956383 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 112481.0 3 51566.0 4 27814.0 5 19607.0 6 16996.0 7 16910.0 8 18657.0 9 21675.0 10 24374.0 11 27217.0 12 29880.0 13 32432.0 14 34783.0 15 36632.0 16 38385.0 17 39898.0 18 38934.0 19 37529.0 20 37563.0 21 38875.0 22 42633.0 23 47927.0 24 55729.0 25 65691.0 26 78087.0 27 92152.0 28 109398.0 29 127846.0 30 150699.0 31 178866.0 32 213993.0 33 261014.0 34 330293.0 35 429109.0 36 570992.0 37 643642.0 38 332873.0 39 19783.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00078282739072 18.4311410041117 13.610688395320858 24.95738777317672 2 16.45622493928162 19.969480803110756 37.606005926428296 25.968288331179323 3 19.792636653940882 23.519431638553602 28.28281875438249 28.40511295312303 4 14.241499210626147 15.717018513955711 34.53879521492778 35.50268706049036 5 15.271119391889798 36.42729866096634 33.18192414613079 15.119657801013068 6 35.11681610579409 33.78456576026013 16.490939681506266 14.60767845243951 7 30.757185229930283 30.644694458918714 19.80779865546317 18.790321655687833 8 27.66450820278483 33.94981352248129 19.168431909229845 19.21724636550403 9 27.2320999876086 15.610604972618598 18.64287817001231 38.514416869760495 10-11 26.62213941044384 24.777265356479415 26.52560935510796 22.07498587796879 12-13 26.963430887538525 21.849265668012336 26.841979004697098 24.34532443975204 14-15 24.077456749417827 23.473356607155605 24.143298696145095 28.305887947281477 16-17 24.53613955796726 26.5906840380412 24.745444598490618 24.127731805500922 18-19 24.50070200523801 25.731048499380925 26.0917153492934 23.67653414608766 20-21 25.227733884968888 24.946318425753855 25.778512003824545 24.047435685452708 22-23 24.95971775796761 24.655957400278837 25.74611208094163 24.638212760811925 24-25 24.639115167892367 24.924843513398066 25.816003169166862 24.620038149542705 26-27 24.443602498960114 25.120713184753924 25.80579014299066 24.629894173295302 28-29 24.498918312151332 25.249927237831248 25.741328833218326 24.50982561679909 30-31 24.71790669567482 25.071950400861304 25.576257429071923 24.63388547439195 32-33 24.49225209819936 25.06906948462601 25.510303222815793 24.92837519435884 34-35 24.793199177143023 25.140264051421713 25.6155956936238 24.450941077811468 36-37 24.85863776968778 24.97676814258127 25.58818660331039 24.57640748442056 38-39 24.806123622613978 25.027046771145677 25.495737662494033 24.67109194374631 40-41 24.72989276576016 24.95545103454906 25.63795488744774 24.67670131224304 42-43 24.78540603841287 24.814705093610986 25.522172276198567 24.877716591777578 44-45 24.582594950144113 24.916712173309662 25.652482146832323 24.8482107297139 46-47 24.648758227990566 24.935509357573682 25.561343220513177 24.854389193922568 48-49 24.558029516467204 24.969267483003772 25.545872050540236 24.926830949988787 50-51 24.44495629671315 25.093472622036618 25.613592889600838 24.847978191649396 52-53 24.637598811039098 24.943429469453708 25.544501533083558 24.874470186423633 54-55 24.392370196447654 24.880636926481152 25.6891367362459 25.037856140825294 56-57 24.341529107535695 24.931651350752567 25.556763431083663 25.170056110628074 58-59 24.316436734886373 24.780148087594913 25.67219955218886 25.23121562532985 60-61 24.323439493350214 24.786633786408377 25.823469312002274 25.066457408239135 62-63 24.142788048471182 24.901698517114482 25.76186667202695 25.193646762387388 64-65 24.117009629151344 24.695454928272646 25.74000729906516 25.44752814351085 66-67 24.062390231907717 24.86601482696982 25.551209233668054 25.52038570745441 68-69 24.145314418291434 24.71544945701746 25.58847646161585 25.550759663075258 70-71 23.931829928371247 24.63003564928258 25.681117829964762 25.757016592381415 72-73 23.912759952257247 24.638505932738887 25.54202345011585 25.906710664888017 74-75 23.715630101828953 24.73340265855053 25.59667131069951 25.954295928921013 76-77 23.696055532169975 24.414502643458913 25.846026750829243 26.04341507354187 78-79 23.59036379622521 24.5333090299067 25.75815228629017 26.118174887577922 80-81 23.462430848638537 24.558228647025395 25.771103659098106 26.20823684523796 82-83 23.418920132961997 24.529197736052467 25.75688392776929 26.29499820321624 84-85 23.26750596819255 24.408587998301122 25.80041384858748 26.52349218491885 86-87 23.10359413937115 24.382965035220135 25.914607200033235 26.59883362537548 88-89 23.03823380983687 24.305454640784976 25.802392017177723 26.853919532200432 90-91 22.907513289847476 24.22519423623233 25.920370350354787 26.946922123565408 92-93 22.808478328188027 24.14829749649204 25.923743546957056 27.119480628362876 94-95 22.589111950417728 24.005060093665183 26.139643942956493 27.266184012960593 96-97 22.555885164820726 23.893553799486025 26.13556305804035 27.414997977652895 98-99 22.382732489388367 23.884334242091764 26.091169816778446 27.641763451741426 100-101 22.450020919830223 23.61145890157304 26.243562071034 27.69495810756274 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 75.0 1 58.5 2 68.5 3 115.0 4 113.0 5 109.5 6 191.0 7 420.0 8 651.0 9 728.0 10 641.5 11 550.5 12 592.0 13 638.5 14 706.5 15 790.5 16 851.5 17 934.0 18 1041.0 19 1154.0 20 1293.5 21 1473.0 22 1712.0 23 2018.0 24 2404.5 25 2988.5 26 3721.0 27 4699.0 28 6000.5 29 7877.0 30 11100.0 31 16318.5 32 23410.5 33 30767.0 34 37166.0 35 44683.0 36 55004.5 37 65354.0 38 75514.5 39 85808.0 40 95996.5 41 106129.5 42 117066.0 43 131112.0 44 144931.5 45 157216.0 46 170254.0 47 182556.5 48 193723.0 49 203851.5 50 209945.0 51 211202.0 52 210502.5 53 211818.5 54 211623.5 55 204068.5 56 188442.0 57 169990.0 58 155602.5 59 140358.0 60 120197.0 61 99688.5 62 81319.0 63 63504.5 64 47091.5 65 34798.0 66 26069.0 67 19511.0 68 14313.5 69 10426.0 70 7846.0 71 5913.5 72 4479.0 73 3716.5 74 2888.0 75 2015.5 76 1358.0 77 765.0 78 408.5 79 236.0 80 130.0 81 70.5 82 38.5 83 22.5 84 12.5 85 4.5 86 3.5 87 3.5 88 3.5 89 2.5 90 1.5 91 1.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1973529817973988 2 0.0 3 0.012508603875870635 4 0.01509117020571825 5 0.007208728625052914 6 0.10660384667640556 7 0.14255766140758847 8 0.12310981408890989 9 0.14156954907269026 10-11 0.11447505970781069 12-13 0.11414943177926469 14-15 0.11593477111163761 16-17 0.06670881115488998 18-19 0.07945321456522496 20-21 0.037885125203938524 22-23 0.08318109291961369 24-25 0.057355429621137516 26-27 0.06566455607369072 28-29 0.04018697780228096 30-31 0.02433226624686864 32-33 0.03232699332013605 34-35 0.019885760739826654 36-37 0.024118923810924706 38-39 0.031147995647814307 40-41 0.02452315158429216 42-43 0.012867917452197258 44-45 0.02703834661857853 46-47 0.03400004715990689 48-49 0.009061439252987075 50-51 0.015618912020947982 52-53 0.006905557795027325 54-55 0.012418775481788977 56-57 0.004794590534108402 58-59 0.003705421255868321 60-61 0.022984840335643797 62-63 0.009735152208599497 64-65 0.00696170054132836 66-67 0.04018697780228096 68-69 0.0895813659979317 70-71 0.02137915779143419 72-73 0.0448580542945271 74-75 0.08660580044397684 76-77 0.2625122531543802 78-79 0.08154172472762347 80-81 0.016730538397708477 82-83 0.012014547708421525 84-85 0.013070031338880987 86-87 0.2712929786758621 88-89 0.6318080097733293 90-91 0.2683847844174685 92-93 0.35664118160269576 94-95 0.0011902262215819455 96-97 0.004221934521837845 98-99 0.0035145359184448007 100-101 0.004985475871531923 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4452935.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.23607721947421 #Duplication Level Percentage of deduplicated Percentage of total 1 69.05802167819371 30.548559795807012 2 12.921849284399054 11.432238455261682 3 6.036640896459656 8.011119385260761 4 3.3704250908263953 5.963775383209994 5 2.0913957891068016 4.625757281170585 6 1.4305153163676474 3.79682315990879 7 0.9889170563009954 3.0622067886330173 8 0.7151881825895822 2.5309695737190543 9 0.5620766476872006 2.237765939131879 >10 2.655921640853302 20.236015376655736 >50 0.12218317234121195 3.6409055855107426 >100 0.04538488313851034 3.361234348445133 >500 0.001123033171678668 0.3152105815578212 >1k 3.573285643030495E-4 0.23741834572774378 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.491419704082813E-5 0.0 0.0 0.0 0.0 3 4.491419704082813E-5 0.0 0.0 0.0 0.0 4 4.491419704082813E-5 0.0 0.0 0.0 0.0 5 4.491419704082813E-5 0.0 0.0 2.2457098520414065E-5 0.0 6 6.737129556124219E-5 0.0 0.0 2.2457098520414065E-5 0.0 7 1.5719968964289844E-4 0.0 0.0 4.491419704082813E-5 0.0 8 1.5719968964289844E-4 0.0 0.0 4.491419704082813E-5 0.0 9 1.5719968964289844E-4 0.0 0.0 4.491419704082813E-5 0.0 10-11 1.9088533742351953E-4 0.0 0.0 6.737129556124219E-5 0.0 12-13 2.470280837245547E-4 0.0 0.0 7.859984482144923E-5 0.0 14-15 2.6948518224496876E-4 0.0 0.0 8.982839408165626E-5 0.0 16-17 2.807137315051758E-4 0.0 0.0 8.982839408165626E-5 0.0 18-19 3.59313576326625E-4 0.0 0.0 8.982839408165626E-5 0.0 20-21 3.8177067484703906E-4 0.0 0.0 1.0105694334186329E-4 0.0 22-23 4.0422777336745314E-4 0.0 0.0 1.9088533742351953E-4 0.0 24-25 5.838845615307657E-4 1.1228549260207032E-5 0.0 5.277418152297305E-4 0.0 26-27 5.838845615307657E-4 2.2457098520414065E-5 0.0 0.0011677691230615314 0.0 28-29 8.870553915563555E-4 2.2457098520414065E-5 0.0 0.005176361208955442 0.0 30-31 0.0010330265319390469 2.2457098520414065E-5 0.0 0.020514559498398248 0.0 32-33 0.0010330265319390469 2.2457098520414065E-5 0.0 0.05324578059190174 0.0 34-35 0.0011228549260207032 2.2457098520414065E-5 0.0 0.09885614768686271 0.0 36-37 0.0013025117141840156 2.2457098520414065E-5 0.0 0.17722019297384758 0.0 38-39 0.001392340108265672 2.2457098520414065E-5 0.0 0.30419262800826874 0.0 40-41 0.001392340108265672 4.491419704082813E-5 0.0 0.4548123877846858 0.0 42-43 0.0014372543053065001 4.491419704082813E-5 2.2457098520414065E-5 0.6148641289396769 0.0 44-45 0.0015270826993881562 4.491419704082813E-5 2.2457098520414065E-5 0.810330714461361 0.0 46-47 0.0017853393323729182 4.491419704082813E-5 2.2457098520414065E-5 1.0419981427979523 0.0 48-49 0.0019088533742351954 4.491419704082813E-5 2.2457098520414065E-5 1.289396768648094 0.0 50-51 0.0019088533742351954 4.491419704082813E-5 2.2457098520414065E-5 1.555266358031276 0.0 52-53 0.0019088533742351954 4.491419704082813E-5 2.2457098520414065E-5 1.8327687244480326 0.0 54-55 0.0019313104727556095 5.614274630103516E-5 2.2457098520414065E-5 2.144664137248803 0.0 56-57 0.0020211388668372656 6.737129556124219E-5 2.2457098520414065E-5 2.5067736223412203 0.0 58-59 0.0020211388668372656 6.737129556124219E-5 2.2457098520414065E-5 2.9018052138645634 0.0 60-61 0.0020211388668372656 6.737129556124219E-5 2.2457098520414065E-5 3.3252787206640115 0.0 62-63 0.0020211388668372656 6.737129556124219E-5 2.2457098520414065E-5 3.7585322938691 0.0 64-65 0.0020211388668372656 6.737129556124219E-5 2.2457098520414065E-5 4.218857899340548 0.0 66-67 0.002178338556480164 6.737129556124219E-5 2.2457098520414065E-5 4.738380416511806 1.1228549260207032E-5 68-69 0.0021895671057403712 6.737129556124219E-5 2.2457098520414065E-5 5.313001424902901 2.2457098520414065E-5 70-71 0.0022457098520414064 6.737129556124219E-5 2.2457098520414065E-5 5.93065023405911 2.2457098520414065E-5 72-73 0.0023243096968628557 6.737129556124219E-5 2.2457098520414065E-5 6.557158368581621 2.2457098520414065E-5 74-75 0.002357995344643477 6.737129556124219E-5 2.2457098520414065E-5 7.208582653912532 2.2457098520414065E-5 76-77 0.002357995344643477 6.737129556124219E-5 2.2457098520414065E-5 7.913398690975727 2.2457098520414065E-5 78-79 0.002369223893903684 7.859984482144923E-5 2.2457098520414065E-5 8.682745200637333 2.2457098520414065E-5 80-81 0.0026948518224496876 8.982839408165626E-5 2.2457098520414065E-5 9.488505895549789 2.2457098520414065E-5 82-83 0.00276222311801093 8.982839408165626E-5 2.2457098520414065E-5 10.31611734732261 2.2457098520414065E-5 84-85 0.002784680216531344 8.982839408165626E-5 3.3685647780621095E-5 11.147479134548337 2.2457098520414065E-5 86-87 0.002818365864311965 8.982839408165626E-5 4.491419704082813E-5 12.022868063423338 2.2457098520414065E-5 88-89 0.002829594413572172 8.982839408165626E-5 4.491419704082813E-5 12.95429868165603 2.2457098520414065E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8050 0.0 44.066505 1 GGTATCA 4520 0.0 41.975914 1 TCAACGC 10570 0.0 32.87187 4 ATCAACG 10645 0.0 32.77404 3 TATCAAC 10850 0.0 32.28351 2 CAACGCA 10795 0.0 31.962551 5 AACGCAG 11330 0.0 30.663076 6 GTACATG 16715 0.0 25.916578 1 ACGCAGA 13595 0.0 25.030787 7 TACATGG 17370 0.0 24.756111 2 CGCAGAG 14190 0.0 23.845825 8 ACATGGG 18205 0.0 22.918514 3 GCAGAGT 16190 0.0 20.137943 9 CATGGGG 13200 0.0 19.633919 4 AGAGTAC 13760 0.0 17.81262 10-11 CAGAGTA 14780 0.0 16.454905 10-11 TACACTG 3780 0.0 16.447851 5 GAGTACA 13280 0.0 16.184752 1 AGTACAT 12615 0.0 16.103142 2 GTATTAA 1705 0.0 15.61814 1 >>END_MODULE