FastQCFastQC Report
Mon 15 Aug 2016
SRR2920858_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920858_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466955
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30830.12497187828720022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83050.035.2451441
GGTATCA51700.031.1572671
TCAACGC104700.027.902824
ATCAACG104700.027.7719753
AACGCAG107650.027.3882456
CAACGCA107350.027.2567445
TATCAAC106300.027.1819292
GTACATG141950.025.062591
TACATGG147300.024.03742
GTATAGT3500.023.6693481
ACATGGG148800.023.1781653
ACGCAGA126100.022.9754267
CGCAGAG128500.022.330918
CATGGGG79700.021.4062184
GCAGAGT131950.020.7040089
GTGTACG1858.614961E-619.9021551
GAGTACT37900.018.3300112-13
CATGGGA67900.018.1017424
GTATTGG7650.017.4469051
GCCGTAT3752.7102942E-1017.17799892
Produced by FastQC (version 0.11.5)