##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920858_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2466955
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.62718168754598	31.0	30.0	33.0	22.0	34.0
2	28.65104916790132	31.0	28.0	34.0	18.0	34.0
3	28.725118617891287	31.0	28.0	34.0	22.0	34.0
4	32.213588411624855	35.0	32.0	37.0	23.0	37.0
5	32.18703340758141	35.0	32.0	37.0	23.0	37.0
6	32.31916269246906	35.0	32.0	37.0	24.0	37.0
7	32.151200569122665	35.0	32.0	37.0	23.0	37.0
8	32.10280933377382	35.0	32.0	37.0	22.0	37.0
9	33.36097415639929	37.0	33.0	39.0	22.0	39.0
10-11	33.15831987206901	37.0	32.5	39.0	19.5	39.0
12-13	32.98930969555586	37.0	32.0	39.0	18.0	39.0
14-15	33.73335387147313	38.0	32.0	40.0	17.5	41.0
16-17	33.630520824255	38.0	32.0	40.0	17.0	41.0
18-19	33.53087206698136	38.0	32.0	40.0	17.5	41.0
20-21	33.45961255880225	38.0	32.0	40.0	18.0	41.0
22-23	33.2369751373657	38.0	32.0	40.0	16.5	41.0
24-25	33.028179881675996	38.0	32.0	40.0	15.0	41.0
26-27	33.07795663074519	38.0	32.0	40.0	13.0	41.0
28-29	32.92289218895358	38.0	32.0	40.0	10.0	41.0
30-31	32.669759278138436	38.0	31.0	40.0	9.5	41.0
32-33	32.42805563944215	37.0	31.0	40.0	9.0	41.0
34-35	32.08839358642537	37.0	30.5	40.0	7.5	41.0
36-37	31.777958252177278	37.0	30.0	40.0	4.0	41.0
38-39	31.58143399453983	37.0	30.0	40.0	2.0	41.0
40-41	31.377274615872604	37.0	30.0	40.0	2.0	41.0
42-43	31.13593154313719	36.5	30.0	40.0	2.0	41.0
44-45	30.846368904175392	36.0	29.0	39.5	2.0	41.0
46-47	30.572682720195544	36.0	28.5	39.0	2.0	40.0
48-49	30.270100994951264	35.5	28.0	39.0	2.0	40.0
50-51	29.113267976108197	34.0	26.0	38.0	2.0	39.5
52-53	29.564978485622966	34.5	27.5	38.5	2.0	39.5
54-55	30.219168570160377	36.0	28.0	39.0	2.0	40.0
56-57	30.17616981258272	35.5	28.0	39.0	2.0	41.0
58-59	29.940506616456318	35.0	28.0	39.0	2.0	41.0
60-61	29.632373918454128	35.0	28.0	39.0	2.0	40.0
62-63	29.251484522417314	35.0	27.0	39.0	2.0	40.0
64-65	28.839887432077198	34.0	26.0	38.0	2.0	40.0
66-67	28.473732800152412	34.0	26.0	38.0	2.0	40.0
68-69	28.034605617046115	34.0	25.5	37.0	2.0	40.0
70-71	27.711166802799404	34.0	25.0	37.0	2.0	39.0
72-73	27.24894556244439	33.5	24.0	36.0	2.0	39.0
74-75	26.804577505467265	33.0	23.0	36.0	2.0	39.0
76-77	26.38316730544335	33.0	22.0	35.0	2.0	37.5
78-79	25.91974215176199	32.0	20.0	35.0	2.0	37.0
80-81	25.469267578857334	32.0	18.5	35.0	2.0	36.5
82-83	24.995220828916622	32.0	17.0	35.0	2.0	36.0
84-85	24.585788350415797	32.0	10.0	35.0	2.0	36.0
86-87	24.132411616750204	31.0	4.5	34.0	2.0	35.0
88-89	23.60878897264036	31.0	2.0	34.0	2.0	35.0
90-91	23.112565287976473	31.0	2.0	34.0	2.0	35.0
92-93	22.576158057200068	30.5	2.0	34.0	2.0	35.0
94-95	21.948860234580685	30.0	2.0	34.0	2.0	35.0
96-97	21.316464021435333	29.5	2.0	34.0	2.0	35.0
98	20.930337602428907	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	124437.0
3	21222.0
4	15524.0
5	12535.0
6	11707.0
7	11876.0
8	13742.0
9	15902.0
10	17413.0
11	18479.0
12	19300.0
13	19467.0
14	18548.0
15	19727.0
16	21649.0
17	23639.0
18	23849.0
19	22692.0
20	23521.0
21	24769.0
22	27280.0
23	30634.0
24	34904.0
25	39934.0
26	45433.0
27	52940.0
28	60735.0
29	71015.0
30	81742.0
31	96211.0
32	114856.0
33	138822.0
34	172829.0
35	218760.0
36	274517.0
37	307541.0
38	196336.0
39	22468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.062382223314565	18.305594562954145	12.953675487108704	23.678347726622587
2	16.04303204576159	20.16532135773216	38.94121997382364	24.850426622682605
3	19.28591758179829	25.134745918700794	28.64815401943001	26.931182480070902
4	13.105924450475381	15.74131546972055	36.46799303153688	34.68476704826719
5	14.251199111258876	37.59297926946453	33.408111384562986	14.747710234713612
6	33.07401846680811	34.70345702248364	17.686998428054824	14.535526082653421
7	29.652172326628406	31.7810585037268	20.800577314070612	17.76619185557418
8	27.170689269347896	33.613718253492074	20.186579144518568	19.02901333264146
9	27.76690292283402	15.644103763546557	19.53663524466397	37.052358068955456
10-11	25.9103744215534	25.611544124085107	27.032690542823694	21.4453909115378
12-13	26.62650280057937	23.066582725632408	27.252655351382813	23.054259122405405
14-15	23.78974942189016	24.327312018786518	25.16549781292165	26.71744074640167
16-17	23.76164534663471	27.62598601474008	25.637508176040203	22.974860462585
18-19	23.511615897318716	26.38693597038327	27.497015963051673	22.604432169246344
20-21	24.493534417184758	25.744500451323038	26.9373725899857	22.824592541506505
22-23	24.332822882481068	25.568880838535225	26.60179315135639	23.49650312762732
24-25	23.92643312444239	25.846730335171298	26.81376445222256	23.413072088163755
26-27	23.635127079288885	26.1367340410794	26.69913517160161	23.529003708030103
28-29	23.44424464261487	26.00334171770942	27.212022234043136	23.340391405632573
30-31	23.59254445671763	25.922579172004216	26.889573997055923	23.595302374222232
32-33	23.460618270469308	26.007810380946626	26.766812854531246	23.764758494052813
34-35	24.007230128028095	26.015892778047352	26.60045285916666	23.3764242347579
36-37	23.971938388321448	25.779991178540833	26.488295268295616	23.759775164842107
38-39	23.821263902730472	25.799790092137087	26.84073597264437	23.53821003248807
40-41	23.78013960812298	25.596499288436835	26.988711113500642	23.63464998993955
42-43	23.90943133838044	25.585589913330146	26.58292726665406	23.92205148163535
44-45	23.61919006919007	25.87657712657713	26.531481481481485	23.972751322751325
46-47	23.64618922022526	25.73999205954002	26.465052513464897	24.14876620676982
48-49	23.945324756345755	25.72848031130444	26.3387299661047	23.9874649662451
50-51	23.508715100531305	25.919509030888634	26.488599072820087	24.083176795759975
52-53	23.639629632419602	25.789364279956978	26.447843943801104	24.12316214382232
54-55	23.289918560909523	25.67746976700248	26.75537062105323	24.27724105103477
56-57	23.427742176440837	25.794710377442016	26.57992084596511	24.197626600152038
58-59	23.40245191472146	25.325802004691205	26.790145616269363	24.48160046431797
60-61	23.419124478879493	25.39927554505597	26.881422352917472	24.30017762314706
62-63	23.29239761202753	25.40955901221112	26.94374809605043	24.35429527971092
64-65	22.982131641337975	25.199465364212998	26.924592126439535	24.8938108680095
66-67	23.19833377182872	25.574211746843446	26.612651637271185	24.614802844056648
68-69	23.17140058486852	25.144400735674964	26.80087015931144	24.883328520145078
70-71	22.923491865095833	25.08947011234419	27.036126558505075	24.950911464054904
72-73	23.167665250424303	25.18707173042469	26.709722115999522	24.93554090315148
74-75	23.13038477088565	25.03123787396982	26.743687549673506	25.09468980547102
76-77	22.980781999884893	25.067228122922476	27.00155555596229	24.95043432123034
78-79	23.15771470314048	24.938298318883398	26.74444522175999	25.15954175621613
80-81	22.849792014267226	25.164758342565957	26.874978515971808	25.110471127195005
82-83	23.107844335649858	25.054309723967688	26.530175582966404	25.30767035741605
84-85	23.057236567262233	24.786858291831766	26.60597126273369	25.54993387817231
86-87	22.966004805034913	24.685897381146155	26.95382993495266	25.394267878866266
88-89	23.127198257832347	24.401109525119345	26.98636652902718	25.48532568802113
90-91	23.03139155745491	24.586710710718855	26.928476554475022	25.453421177351217
92-93	23.287559231151285	24.405634286360474	26.813950830161527	25.492855652326718
94-95	23.14492462376973	24.10452843175622	26.99725246338584	25.75329448108821
96-97	23.30814986091596	23.976721424311677	26.99619563050753	25.718933084264833
98	23.097424724580296	23.916799107039346	27.056480005402022	25.92929616297834
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	79.0
2	124.0
3	203.5
4	196.5
5	213.0
6	366.5
7	686.0
8	1069.5
9	1206.0
10	1164.5
11	1169.0
12	1236.0
13	1354.0
14	1528.0
15	1667.5
16	1719.5
17	1783.5
18	1821.5
19	1848.5
20	1872.5
21	1951.0
22	2148.0
23	2500.0
24	2912.5
25	3518.0
26	4510.0
27	5847.0
28	7908.5
29	10759.0
30	14833.5
31	20207.0
32	26533.0
33	32754.5
34	39321.5
35	47521.0
36	56270.0
37	62587.0
38	65865.5
39	68665.0
40	72216.5
41	76822.0
42	80938.0
43	85345.0
44	88548.5
45	89837.0
46	92746.5
47	100001.5
48	106161.0
49	107687.5
50	108307.0
51	106888.5
52	104876.5
53	103163.0
54	99305.5
55	93775.5
56	86597.5
57	77997.5
58	70449.5
59	62292.5
60	53616.0
61	44687.0
62	36609.5
63	29290.0
64	22347.0
65	17455.5
66	13528.0
67	10600.0
68	7748.0
69	5700.0
70	4759.5
71	4383.5
72	3855.5
73	2885.5
74	2124.0
75	1418.0
76	899.0
77	523.0
78	307.0
79	195.5
80	129.5
81	93.0
82	67.5
83	37.0
84	22.0
85	16.0
86	9.5
87	7.0
88	4.5
89	2.5
90	4.0
91	4.0
92	1.5
93	1.0
94	2.5
95	2.5
96	1.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3828201162972166
2	0.02460523195599433
3	0.018687004829840835
4	0.01678182212484622
5	0.022092012217490794
6	0.023672908504613985
7	0.014592888804214102
8	0.00920162710710167
9	0.0
10-11	1.2160740670178418E-4
12-13	0.00583715552168564
14-15	0.0032225962775972805
16-17	0.005816887620568676
18-19	0.03715106274739507
20-21	0.08026088842317756
22-23	0.10000182411110052
24-25	0.06621523294912149
26-27	0.07699775634334635
28-29	0.05904039595371621
30-31	0.053770741663305574
32-33	0.02002468630356046
34-35	0.33863609186223503
36-37	0.5153113858988104
38-39	0.4698910194956941
40-41	0.47820085895364933
42-43	0.42824048270033294
44-45	0.4035339112387538
46-47	0.40205435445721543
48-49	0.3924676372288915
50-51	0.3895895952702826
52-53	0.4487515986307006
54-55	0.4487515986307006
56-57	0.4196468926267402
58-59	0.40608766677949126
60-61	0.4195252852200385
62-63	0.4009598877968994
64-65	0.4490353492463381
66-67	0.46976941208899226
68-69	0.41198562600452787
70-71	0.3709431262426757
72-73	0.35699881027420444
74-75	0.34054127456722966
76-77	0.33841314494994845
78-79	0.3385144844555332
80-81	0.3540194288100107
82-83	0.3486687029151322
84-85	0.34917540044305634
86-87	0.3357985857058601
88-89	0.378077427435847
90-91	0.35712041768090624
92-93	0.34771611156263493
94-95	0.35480987695357236
96-97	0.353837017699958
98	0.34925647204752414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2466955.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.0865311025341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.69336732031478	29.867182973540483
2	10.677706610920035	8.774198495465999
3	5.01759645824189	6.184668988245611
4	2.9741746182264843	4.887940718245198
5	1.9030643502659348	3.9095156308662595
6	1.2953077665530244	3.193182170270089
7	0.9465317900317343	2.7222795481473288
8	0.7031564092827781	2.311220614395445
9	0.5707221287835423	2.1104093245652535
>10	2.911436946285001	21.20108079414851
>50	0.18907344932367706	5.34798115085166
>100	0.11346727540945492	8.119072892243636
>500	0.0037955750396559194	0.9625624117252481
>1k	5.993013220508392E-4	0.40870428728929764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3083	0.12497187828720022	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.053580223392806E-5	0.0	0.0	0.0	0.0
6	4.053580223392806E-5	0.0	0.0	0.0	0.0
7	2.0267901116964032E-4	0.0	0.0	4.053580223392806E-5	0.0
8	2.0267901116964032E-4	0.0	0.0	4.053580223392806E-5	0.0
9	2.0267901116964032E-4	0.0	0.0	4.053580223392806E-5	0.0
10-11	2.0267901116964032E-4	0.0	0.0	4.053580223392806E-5	0.0
12-13	2.837506156374964E-4	0.0	0.0	4.053580223392806E-5	0.0
14-15	3.242864178714245E-4	0.0	0.0	8.107160446785612E-5	0.0
16-17	3.445543189883885E-4	0.0	0.0	8.107160446785612E-5	0.0
18-19	4.458938245732087E-4	0.0	0.0	1.0133950558482014E-4	0.0
20-21	5.269654290410648E-4	0.0	0.0	2.634827145205324E-4	0.0
22-23	5.269654290410648E-4	0.0	0.0	6.28304934625885E-4	0.0
24-25	7.296444402107051E-4	0.0	0.0	0.0014592888804214102	0.0
26-27	7.499123413276692E-4	0.0	0.0	0.002756434551907108	0.0
28-29	8.309839457955252E-4	0.0	0.0	0.008553054271358822	0.0
30-31	8.917876491464174E-4	0.0	0.0	0.032185426973738884	0.0
32-33	8.917876491464174E-4	0.0	0.0	0.0810310686656222	0.0
34-35	9.728592536142734E-4	0.0	0.0	0.15063104110127667	0.0
36-37	0.0010539308580821297	0.0	0.0	0.2639894120484565	0.0
38-39	0.0010944666603160576	0.0	0.0	0.44408998137379885	0.0
40-41	0.0010944666603160576	0.0	0.0	0.6622334010956827	0.0
42-43	0.0012363419681348058	0.0	0.0	0.8788769961349112	0.0
44-45	0.001378217275953554	0.0	0.0	1.1440824822503854	0.0
46-47	0.0016416999904740864	0.0	0.0	1.4682270248139915	0.0
48-49	0.0017430394960589067	0.0	0.0	1.8207466289413468	0.0
50-51	0.0017430394960589067	0.0	0.0	2.203343798326277	0.0
52-53	0.0017835752982928348	0.0	0.0	2.590278298550237	0.0
54-55	0.0018241111005267627	0.0	0.0	3.0156002034897273	0.0
56-57	0.001986254309462475	0.0	0.0	3.5107044919749244	0.0
58-59	0.001986254309462475	0.0	0.0	4.044642079000225	0.0
60-61	0.002026790111696403	0.0	0.0	4.624526997857682	0.0
62-63	0.002026790111696403	0.0	0.0	5.199385477238133	0.0
64-65	0.002026790111696403	0.0	0.0	5.8023960712700475	0.0
66-67	0.002188933320632115	0.0	0.0	6.487653808034601	0.0
68-69	0.0022294691228660433	0.0	0.0	7.232641049390848	0.0
70-71	0.0022902728262169355	0.0	0.0	8.058598555709366	0.0
72-73	0.0023510765295678276	0.0	0.0	8.882002306487147	0.0
74-75	0.0023713444306847917	0.0	0.0	9.704007572087857	0.0
76-77	0.0023916123318017558	0.0	0.0	10.596139775553263	0.0
78-79	0.0023916123318017558	0.0	0.0	11.55943257984033	0.0
80-81	0.002594291342971396	0.0	0.0	12.59200107014518	0.0
82-83	0.002634827145205324	0.0	0.0	13.637399141857067	0.0
84-85	0.00271589874967318	0.0	0.0	14.657746087788386	0.0
86	0.002796970354141036	0.0	0.0	15.427480436408446	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8305	0.0	35.245144	1
GGTATCA	5170	0.0	31.157267	1
TCAACGC	10470	0.0	27.90282	4
ATCAACG	10470	0.0	27.771975	3
AACGCAG	10765	0.0	27.388245	6
CAACGCA	10735	0.0	27.256744	5
TATCAAC	10630	0.0	27.181929	2
GTACATG	14195	0.0	25.06259	1
TACATGG	14730	0.0	24.0374	2
GTATAGT	350	0.0	23.669348	1
ACATGGG	14880	0.0	23.178165	3
ACGCAGA	12610	0.0	22.975426	7
CGCAGAG	12850	0.0	22.33091	8
CATGGGG	7970	0.0	21.406218	4
GCAGAGT	13195	0.0	20.704008	9
GTGTACG	185	8.614961E-6	19.902155	1
GAGTACT	3790	0.0	18.33001	12-13
CATGGGA	6790	0.0	18.101742	4
GTATTGG	765	0.0	17.446905	1
GCCGTAT	375	2.7102942E-10	17.177998	92
>>END_MODULE