FastQCFastQC Report
Mon 15 Aug 2016
SRR2920858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466955
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28810.11678364623594674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82250.035.456381
GGTATCA50950.035.2953451
TCAACGC106200.026.9580674
ATCAACG106850.026.9273763
CAACGCA107600.026.602455
AACGCAG109350.026.2635356
TATCAAC110900.025.9424272
GTACATG143900.025.0928961
TACATGG148600.023.8335912
ACATGGG151700.022.628133
ACGCAGA128900.022.0960647
CGCAGAG130450.021.612558
CATGGGG79850.021.048654
GAGTACT40950.019.7589412-13
GCAGAGT136800.019.568469
AGTACAT110400.017.932322
CTAGACT6700.017.7158154
AGTACTT44300.017.67556812-13
GAGTACA120400.017.4253671
GCTTATA14500.017.0610281
Produced by FastQC (version 0.11.5)