##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920858_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2466955
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.23400670056811	31.0	30.0	34.0	26.0	34.0
2	30.255158687531797	31.0	30.0	34.0	26.0	34.0
3	30.387973027477194	31.0	30.0	34.0	27.0	34.0
4	34.03600957455649	35.0	35.0	37.0	30.0	37.0
5	33.571226066142266	35.0	35.0	37.0	28.0	37.0
6	33.52005366940216	35.0	35.0	37.0	28.0	37.0
7	33.33159015871794	35.0	33.0	37.0	27.0	37.0
8	33.26381916167907	35.0	33.0	37.0	27.0	37.0
9	34.58283633061811	38.0	34.0	39.0	27.0	39.0
10-11	34.386854239335534	37.5	34.0	39.0	27.0	39.0
12-13	34.20753195741308	37.0	34.0	39.0	26.0	39.0
14-15	35.07933748284829	38.0	34.0	40.0	26.0	41.0
16-17	34.959725451011465	38.0	34.0	40.0	25.0	41.0
18-19	34.8895190629744	38.5	34.0	40.0	25.0	41.0
20-21	34.791274668569145	38.0	34.0	40.0	25.0	41.0
22-23	34.6415441303145	38.0	33.5	40.0	24.5	41.0
24-25	34.448705387816155	38.0	33.0	40.0	24.0	41.0
26-27	34.26817068004888	38.0	33.0	40.0	23.0	41.0
28-29	34.20261030298485	38.0	33.0	40.0	22.5	41.0
30-31	34.18157607252666	38.0	33.0	40.0	23.0	41.0
32-33	34.03119088106593	38.0	33.0	40.0	22.0	41.0
34-35	33.845419961045096	38.0	33.0	40.0	20.5	41.0
36-37	33.64078165187448	38.0	33.0	40.0	19.0	41.0
38-39	33.44529916435444	38.0	32.0	40.0	18.0	41.0
40-41	33.210813330603926	38.0	32.0	40.0	16.0	41.0
42-43	33.21522240981291	38.0	32.0	40.0	15.5	41.0
44-45	33.0377027550158	38.0	32.0	40.0	15.0	41.0
46-47	32.76368985246995	37.5	31.5	40.0	11.0	41.0
48-49	32.47830686007649	37.0	31.0	40.0	9.0	41.0
50-51	32.21498912627105	37.0	31.0	40.0	8.0	41.0
52-53	31.955115314223406	37.0	30.5	40.0	7.0	41.0
54-55	31.67165088134968	36.0	30.0	40.0	3.5	41.0
56-57	31.348866720309047	36.0	30.0	39.0	2.0	41.0
58-59	31.011181801046227	35.5	29.0	39.0	2.0	40.5
60-61	30.683585229564383	35.0	29.0	39.0	2.0	40.0
62-63	30.36495254270953	35.0	28.5	39.0	2.0	40.0
64-65	29.95842587319185	35.0	28.0	38.0	2.0	40.0
66-67	29.531845331592997	34.0	28.0	38.0	2.0	40.0
68-69	29.096615463192478	34.0	27.0	37.0	2.0	39.5
70-71	28.679227428145225	34.0	26.0	36.5	2.0	39.0
72-73	28.243406750427148	34.0	26.0	36.0	2.0	39.0
74-75	27.79932264674467	33.0	26.0	36.0	2.0	38.5
76-77	26.355870698898034	31.5	24.0	34.5	2.0	36.0
78-79	27.18395552411779	33.0	25.5	35.0	2.0	37.0
80-81	27.20962887446265	33.0	26.0	35.0	2.0	37.0
82-83	26.87371881530064	33.0	25.0	35.0	2.0	36.0
84-85	26.546803650654347	33.0	24.5	35.0	2.0	36.0
86-87	26.00075356056353	32.0	23.0	35.0	2.0	35.0
88-89	25.636352507443387	32.0	21.5	35.0	2.0	35.0
90-91	25.266808677093827	32.0	20.0	35.0	2.0	35.0
92-93	24.94277945888758	32.0	18.5	34.0	2.0	35.0
94-95	24.563635939852976	32.0	14.0	34.0	2.0	35.0
96-97	24.037086205463822	31.0	4.5	34.0	2.0	35.0
98-99	23.452028715562303	31.0	2.0	34.0	2.0	35.0
100-101	21.297058316831887	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57068.0
3	26123.0
4	14301.0
5	10117.0
6	8829.0
7	8915.0
8	10014.0
9	11674.0
10	12953.0
11	14847.0
12	16560.0
13	17902.0
14	19892.0
15	21575.0
16	23557.0
17	24142.0
18	23234.0
19	21214.0
20	21174.0
21	21797.0
22	23813.0
23	26912.0
24	31211.0
25	36116.0
26	42590.0
27	50197.0
28	59008.0
29	69497.0
30	82681.0
31	96982.0
32	115560.0
33	141758.0
34	178172.0
35	229881.0
36	304011.0
37	357757.0
38	217474.0
39	17446.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73862763597875	19.78838656432172	13.774246780532797	23.69873901916673
2	16.081079711628302	21.357381873605316	37.951847520526314	24.609690894240067
3	19.022758147905055	25.982688450899733	29.047905339856005	25.9466480613392
4	13.48457458431832	16.742810276934627	36.032232078750965	33.740383059996084
5	14.265149893628063	37.99152429690743	33.3306578196347	14.412667989829805
6	32.97717878525464	35.27829156193721	17.38479218727047	14.359737465537686
7	29.440906448606324	32.004785225069575	20.745907309199907	17.80840101712419
8	27.12002603967967	33.97804809548315	20.170767699757945	18.731158165079233
9	27.478502735794024	15.6330565267533	19.490731099801785	37.39770963765089
10-11	25.91411053899447	25.81070749112065	27.02885870306281	21.246323266822067
12-13	26.584519509949722	23.118020692626093	27.374742529212824	22.92271726821136
14-15	23.727010356639408	24.45844205413682	25.10929573269282	26.70525185653095
16-17	23.753641958608124	27.85364670435956	25.5510243319138	22.841687005118516
18-19	23.57230497926279	26.47748176632704	27.45177663327199	22.49843662113818
20-21	24.54184165189434	25.732555595678335	26.891457422756265	22.834145329671056
22-23	24.28977647305583	25.71146090317105	26.593142176962655	23.40562044681047
24-25	23.930067615662693	26.00799901685342	26.804493758601012	23.257439608882876
26-27	23.68331922443372	26.275834598537244	26.65963452609308	23.381211650935953
28-29	23.515793417908778	26.088985889493927	27.14524624041551	23.249974452181785
30-31	23.696106463656328	26.032621564550606	26.850046060079208	23.421225911713854
32-33	23.428997533042793	26.194714450919555	26.695789791241246	23.680498224796406
34-35	23.973285173234657	26.175559614846243	26.624942149701873	23.226213062217223
36-37	23.958604482568198	25.924684777611002	26.503900088957224	23.612810650863583
38-39	23.805408057547538	25.818172971066062	26.91730176977335	23.45911720161305
40-41	23.78534450041682	25.63398716131397	27.061593580008235	23.51907475826097
42-43	23.97286903416324	25.60921225251639	26.58445051300804	23.83346820031233
44-45	23.61389555928805	25.890661317282476	26.61086169618593	23.884581427243543
46-47	23.631540863142224	25.761650643992102	26.52168438902812	24.085124103837554
48-49	23.883240571562236	25.732381513374275	26.419267259709585	23.965110655353904
50-51	23.47034960657503	25.90683563723169	26.50530458812006	24.117510168073224
52-53	23.639594185402373	25.7472713036347	26.506313971002417	24.10682053996051
54-55	23.282207084214594	25.674531214336593	26.655603029061005	24.387658672387815
56-57	23.422585278232813	25.87939535526668	26.365949594561783	24.332069771938723
58-59	23.28741662471046	25.484361077938004	26.656256663334492	24.571965634017037
60-61	23.459093193446833	25.68900054148174	26.54132239530762	24.31058386976381
62-63	23.171066409859016	25.782635645967055	26.566416548120824	24.479881396053102
64-65	22.794015554401998	25.534045346499855	26.61392274851055	25.058016350587604
66-67	23.089509822527823	25.84891750244529	26.27777615569408	24.783796519332803
68-69	23.060641118723144	25.47322971585018	26.405478715084783	25.060650450341893
70-71	22.743563311712993	25.380352757176116	26.584483022509275	25.291600908601612
72-73	22.983458484403073	25.55072993018941	26.232776731613562	25.233034853793963
74-75	22.95102119556651	25.41755896904109	26.09381209222618	25.53760774316622
76-77	22.74745396162839	25.489031981216492	26.45382834284618	25.309685714308934
78-79	22.881312098161523	25.464782230090606	26.136364788862952	25.51754088288492
80-81	22.48657342220734	25.687403889541844	26.1752487165339	25.650773971716916
82-83	22.722887907854776	25.690794254524775	25.82230751034339	25.76401032727706
84-85	22.542071230211015	25.449151683457323	25.843350292907385	26.165426793424277
86-87	22.464184266153914	25.436212451345103	26.085465979472787	26.014137303028196
88-89	22.46599742748827	25.282830472618983	26.07108281860325	26.180089281289494
90-91	22.290300030076164	25.50288162183692	25.981438128449614	26.225380219637294
92-93	22.372674862634987	25.413000125085293	25.83180197065275	26.382523041626964
94-95	22.169502422149396	25.166498069151398	26.018390134582773	26.645609374116436
96-97	22.21464620872773	25.110485031517925	25.999087905628638	26.67578085412571
98-99	21.933336184449082	25.13741600947435	25.952537840960844	26.97670996511572
100-101	22.0700090196913	25.164249592086996	26.000749951861195	26.764991436360503
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	105.0
1	86.0
2	116.5
3	166.0
4	147.5
5	154.0
6	266.0
7	574.5
8	881.0
9	915.5
10	732.0
11	589.0
12	616.5
13	706.5
14	777.0
15	832.0
16	885.5
17	949.0
18	1078.0
19	1179.5
20	1315.5
21	1496.5
22	1699.0
23	2060.5
24	2459.0
25	2934.5
26	3698.5
27	4827.5
28	6466.5
29	8774.5
30	12093.5
31	16450.5
32	21989.0
33	27832.0
34	33457.5
35	40404.0
36	49372.5
37	57423.5
38	61940.5
39	65254.5
40	67913.0
41	71742.5
42	77204.0
43	82171.0
44	85705.0
45	88048.0
46	90065.5
47	93530.0
48	100556.5
49	106478.0
50	107923.5
51	106556.5
52	103883.5
53	101833.0
54	98435.0
55	92809.5
56	85171.5
57	76555.0
58	69795.5
59	62050.0
60	53673.0
61	45001.0
62	36871.5
63	29403.5
64	22433.5
65	17707.0
66	13710.5
67	10449.0
68	7717.5
69	5604.5
70	4627.0
71	4195.5
72	3920.0
73	3309.0
74	2297.5
75	1555.0
76	1002.0
77	549.5
78	335.0
79	193.0
80	113.5
81	78.5
82	48.0
83	28.0
84	17.0
85	12.0
86	9.0
87	4.0
88	3.5
89	2.5
90	1.0
91	1.0
92	0.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19554470997646894
2	0.0
3	0.011268953021032
4	0.013944315968471253
5	0.005634476510516
6	0.108190056162354
7	0.1434562041058714
8	0.12253973015316454
9	0.14195637942321607
10-11	0.11394614007957178
12-13	0.11514194624547265
14-15	0.11688498574153157
16-17	0.06453299715641347
18-19	0.07870026003717133
20-21	0.03702945534069328
22-23	0.08358482420635967
24-25	0.05683119473196714
26-27	0.06518156999215632
28-29	0.04017098001382271
30-31	0.024767375164930043
32-33	0.030766673895551398
34-35	0.018626201126489945
36-37	0.024362017142590765
38-39	0.03323935783182101
40-41	0.02671309367215859
42-43	0.013255207330494477
44-45	0.025436215901789856
46-47	0.0330164109195344
48-49	0.009809664140610592
50-51	0.016660214718144432
52-53	0.0066276036652472385
54-55	0.012809313505921268
56-57	0.0047832246636035115
58-59	0.003931972816691022
60-61	0.02328781838339167
62-63	0.01045823697635344
64-65	0.007843677732265079
66-67	0.04017098001382271
68-69	0.08984760565150154
70-71	0.021382635678397054
72-73	0.04331250468695213
74-75	0.08607777604374624
76-77	0.2461739269666451
78-79	0.07930829707068025
80-81	0.0158900344756998
82-83	0.011491899933318605
84-85	0.01236341968134806
86-87	0.26405021575180737
88-89	0.6187992890020289
90-91	0.2649014675987199
92-93	0.349925312784384
94-95	0.0013984851770705183
96-97	0.004256259234562447
98-99	0.003425275288766921
100-101	0.005269654290410648
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2466955.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.99084028111132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03227728012142	26.605850606715936
2	11.381903100603491	8.648161255802258
3	5.54195499602527	6.316305812973087
4	3.2525928112902753	4.942749359728787
5	2.087388870489289	3.965082859166396
6	1.5179230174242326	3.4600302548391944
7	1.058970064981526	2.8161813820833856
8	0.8213225567071867	2.4962187256909374
9	0.6383260147114118	2.18254875049616
>10	3.3084532235917035	22.310344870997543
>50	0.22193770189480097	5.827801662117401
>100	0.13211654655441435	8.886591339498008
>500	0.0039744706085506915	0.9832940147601981
>1k	8.593449964433398E-4	0.5588391051307532
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2881	0.11678364623594674	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.053580223392806E-5
2	4.053580223392806E-5	0.0	0.0	0.0	4.053580223392806E-5
3	4.053580223392806E-5	0.0	0.0	0.0	4.053580223392806E-5
4	4.053580223392806E-5	0.0	0.0	0.0	4.053580223392806E-5
5	4.053580223392806E-5	0.0	0.0	0.0	4.053580223392806E-5
6	1.2160740670178418E-4	0.0	0.0	0.0	4.053580223392806E-5
7	2.837506156374964E-4	0.0	0.0	4.053580223392806E-5	4.053580223392806E-5
8	2.837506156374964E-4	0.0	0.0	4.053580223392806E-5	4.053580223392806E-5
9	2.837506156374964E-4	0.0	0.0	4.053580223392806E-5	4.053580223392806E-5
10-11	2.837506156374964E-4	0.0	0.0	4.053580223392806E-5	4.053580223392806E-5
12-13	2.837506156374964E-4	0.0	0.0	4.053580223392806E-5	4.053580223392806E-5
14-15	3.6482222010535256E-4	0.0	0.0	8.107160446785612E-5	4.053580223392806E-5
16-17	3.850901212223166E-4	0.0	0.0	8.107160446785612E-5	4.053580223392806E-5
18-19	4.661617256901727E-4	0.0	0.0	1.0133950558482014E-4	4.053580223392806E-5
20-21	4.864296268071367E-4	0.0	0.0	3.040185167544605E-4	4.053580223392806E-5
22-23	5.269654290410648E-4	0.0	0.0	7.296444402107051E-4	4.053580223392806E-5
24-25	6.89108637976777E-4	0.0	0.0	0.0014998246826553384	4.053580223392806E-5
26-27	6.89108637976777E-4	0.0	0.0	0.002796970354141036	4.053580223392806E-5
28-29	8.512518469124893E-4	0.0	0.0	0.008471982666890965	4.053580223392806E-5
30-31	8.917876491464174E-4	0.0	0.0	0.03161792574246389	4.053580223392806E-5
32-33	8.917876491464174E-4	0.0	0.0	0.08048383533546416	4.053580223392806E-5
34-35	9.120555502633815E-4	0.0	0.0	0.14984059295771507	4.053580223392806E-5
36-37	9.728592536142734E-4	0.0	0.0	0.2629962848937253	4.053580223392806E-5
38-39	0.0010944666603160576	0.0	0.0	0.4430360505157167	4.053580223392806E-5
40-41	0.0010944666603160576	0.0	0.0	0.6621320615900979	4.053580223392806E-5
42-43	0.0011755382647839138	0.0	0.0	0.880721375136555	4.053580223392806E-5
44-45	0.001378217275953554	0.0	0.0	1.1465551661866553	6.080370335089209E-5
46-47	0.0014592888804214102	0.0	0.0	1.4709834593658984	8.107160446785612E-5
48-49	0.0014592888804214102	0.0	0.0	1.8206655573368788	8.107160446785612E-5
50-51	0.0014592888804214102	0.0	0.0	2.199736111927457	8.107160446785612E-5
52-53	0.0014795567815383743	0.0	0.0	2.583508819577171	8.107160446785612E-5
54-55	0.0014998246826553382	0.0	0.0	3.0029124973905077	8.107160446785612E-5
56-57	0.0015808962871231944	0.0	0.0	3.490578466165779	8.107160446785612E-5
58-59	0.0015808962871231944	0.0	0.0	4.015517105095148	8.107160446785612E-5
60-61	0.0016011641882401585	0.0	0.0	4.587659685725925	8.107160446785612E-5
62-63	0.0016214320893571225	0.0	0.0	5.156640473782456	8.107160446785612E-5
64-65	0.0016214320893571225	0.0	0.0	5.750348101201684	8.107160446785612E-5
66-67	0.0017025036938249785	0.0	0.0	6.424985457780949	8.107160446785612E-5
68-69	0.0017227715949419426	0.0	0.0	7.159879284380947	8.107160446785612E-5
70-71	0.0017835752982928348	0.0	0.0	7.973230156204714	8.107160446785612E-5
72-73	0.0018646469027606908	0.0	0.0	8.785344686060345	8.107160446785612E-5
74-75	0.001905182704994619	0.0	0.0	9.601593867743837	8.107160446785612E-5
76-77	0.001905182704994619	0.0	0.0	10.484990605827832	8.107160446785612E-5
78-79	0.001905182704994619	0.0	0.0	11.437156332401685	8.107160446785612E-5
80-81	0.002209201221749079	0.0	0.0	12.461070428929592	8.107160446785612E-5
82-83	0.0022294691228660433	0.0	0.0	13.499780093272882	8.107160446785612E-5
84-85	0.0022700049250999714	0.0	0.0	14.510114696052423	8.107160446785612E-5
86-87	0.0022700049250999714	0.0	0.0	15.546473283866142	8.107160446785612E-5
88-89	0.0022700049250999714	0.0	0.0	16.66043766505672	8.107160446785612E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8225	0.0	35.45638	1
GGTATCA	5095	0.0	35.295345	1
TCAACGC	10620	0.0	26.958067	4
ATCAACG	10685	0.0	26.927376	3
CAACGCA	10760	0.0	26.60245	5
AACGCAG	10935	0.0	26.263535	6
TATCAAC	11090	0.0	25.942427	2
GTACATG	14390	0.0	25.092896	1
TACATGG	14860	0.0	23.833591	2
ACATGGG	15170	0.0	22.62813	3
ACGCAGA	12890	0.0	22.096064	7
CGCAGAG	13045	0.0	21.61255	8
CATGGGG	7985	0.0	21.04865	4
GAGTACT	4095	0.0	19.75894	12-13
GCAGAGT	13680	0.0	19.56846	9
AGTACAT	11040	0.0	17.93232	2
CTAGACT	670	0.0	17.715815	4
AGTACTT	4430	0.0	17.675568	12-13
GAGTACA	12040	0.0	17.425367	1
GCTTATA	1450	0.0	17.061028	1
>>END_MODULE