FastQCFastQC Report
Mon 15 Aug 2016
SRR2920857_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920857_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304328
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49300.042.012341
GGTATCA26100.041.7944951
TCAACGC61600.033.430434
CAACGCA63650.032.3537255
ATCAACG64400.032.0481533
TATCAAC66600.030.9220432
AACGCAG67350.030.707866
GTACATG103700.026.408851
ACGCAGA78750.025.557567
TACATGG104150.025.3223882
ACATGGG107300.023.8939763
CGCAGAG84300.023.5479228
GAGTACA75400.021.548381
GCAGAGT92650.020.6339749
CATGGGG77000.019.7157634
AGTACAT77900.019.6066272
AGAGTAC78500.017.87093510-11
GTCTTAG11650.016.9884511
TAAGGTG15250.016.8420335
CAGAGTA85800.016.53746410-11
Produced by FastQC (version 0.11.5)