##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920857_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3304328
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.79101015395566	31.0	30.0	33.0	23.0	34.0
2	28.816235857941464	31.0	29.0	34.0	22.0	34.0
3	28.89692367101571	31.0	29.0	34.0	22.0	34.0
4	32.38761950992759	35.0	33.0	37.0	25.0	37.0
5	32.355535830583406	35.0	33.0	37.0	25.0	37.0
6	32.48553382109766	35.0	33.0	37.0	25.0	37.0
7	32.3214196653601	35.0	33.0	37.0	23.0	37.0
8	32.26393687309492	35.0	32.0	37.0	23.0	37.0
9	33.549278703566955	37.0	33.0	39.0	23.0	39.0
10-11	33.36631215182028	37.0	33.0	39.0	22.0	39.0
12-13	33.19609357787726	37.0	33.0	39.0	20.0	39.0
14-15	33.98699281669374	38.0	33.0	40.0	19.0	41.0
16-17	33.88070009999008	38.0	33.0	40.0	18.0	41.0
18-19	33.76600749078179	38.0	33.0	40.0	18.0	41.0
20-21	33.66534178810336	38.0	32.0	40.0	18.0	41.0
22-23	33.4500203974908	38.0	32.0	40.0	17.5	41.0
24-25	33.2551508203786	38.0	32.0	40.0	16.0	41.0
26-27	33.29045648615997	38.0	32.0	40.0	16.0	41.0
28-29	33.13951747526275	38.0	32.0	40.0	14.0	41.0
30-31	32.8955241428817	38.0	31.5	40.0	10.0	41.0
32-33	32.65745031970192	38.0	31.0	40.0	9.0	41.0
34-35	32.33447723712658	37.5	30.5	40.0	8.5	41.0
36-37	32.0357876094625	37.0	30.0	40.0	8.0	41.0
38-39	31.831545930065055	37.0	30.0	40.0	4.5	41.0
40-41	31.634745400577664	37.0	30.0	40.0	2.0	41.0
42-43	31.39401914700962	37.0	30.0	40.0	2.0	41.0
44-45	31.11650735035989	36.0	29.5	40.0	2.0	41.0
46-47	30.866198815613945	36.0	29.0	39.0	2.0	41.0
48-49	30.560254762844366	36.0	28.0	39.0	2.0	40.0
50-51	29.394540433032073	34.5	27.0	38.0	2.0	39.5
52-53	29.827623347319033	34.5	28.0	38.5	2.0	39.5
54-55	30.481496540295034	36.0	28.5	39.0	2.0	40.0
56-57	30.424505073346232	36.0	28.0	39.0	2.0	41.0
58-59	30.180823907311865	35.0	28.0	39.0	2.0	41.0
60-61	29.85389328783341	35.0	28.0	39.0	2.0	40.0
62-63	29.457676417111134	35.0	27.5	38.5	2.0	40.0
64-65	29.037960668553485	34.0	26.5	38.0	2.0	40.0
66-67	28.648362087540946	34.0	26.0	37.5	2.0	40.0
68-69	28.197454520253437	34.0	26.0	37.0	2.0	39.5
70-71	27.869179905868908	34.0	26.0	36.5	2.0	39.0
72-73	27.409704333226	33.5	25.0	36.0	2.0	39.0
74-75	26.97199854251757	33.0	24.0	35.5	2.0	38.5
76-77	26.556930335003063	33.0	23.0	35.0	2.0	37.5
78-79	26.107361163903825	32.5	21.5	35.0	2.0	37.0
80-81	25.65985186700594	32.0	20.0	35.0	2.0	36.0
82-83	25.192675938950373	32.0	18.5	35.0	2.0	36.0
84-85	24.799575435610507	32.0	16.5	35.0	2.0	35.5
86-87	24.34108448071741	31.5	8.5	34.0	2.0	35.0
88-89	23.80399585029089	31.0	3.5	34.0	2.0	35.0
90-91	23.30499847472769	31.0	2.0	34.0	2.0	35.0
92-93	22.768716816248265	31.0	2.0	34.0	2.0	35.0
94-95	22.135446602153298	30.0	2.0	34.0	2.0	35.0
96-97	21.49379722594125	30.0	2.0	34.0	2.0	35.0
98	21.09638964412734	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	157249.0
3	27509.0
4	20516.0
5	16772.0
6	15622.0
7	15919.0
8	18020.0
9	20704.0
10	22475.0
11	24416.0
12	25901.0
13	25538.0
14	24210.0
15	24960.0
16	26913.0
17	28922.0
18	29639.0
19	29276.0
20	30516.0
21	32587.0
22	35730.0
23	39830.0
24	45574.0
25	51775.0
26	60275.0
27	69583.0
28	81141.0
29	94415.0
30	110353.0
31	129010.0
32	154037.0
33	188289.0
34	234236.0
35	300026.0
36	382858.0
37	424793.0
38	257384.0
39	27352.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.9787837215768	17.33223339881286	12.602727655301182	25.086255224309163
2	16.281139490265005	19.04610403330829	38.86594648854135	25.806809987885366
3	20.051009875671753	22.620966838737722	28.401591682472304	28.926431603118225
4	13.565993423858444	14.732391356396542	35.50704787664725	36.19456734309776
5	14.763299495335975	36.487856824588086	33.71910627972529	15.029737400350648
6	34.89168572984502	33.90860390332766	16.621002824589397	14.578707542237918
7	30.35208639360632	30.971047257324454	19.92845733789016	18.74840901117907
8	27.45499995308633	34.127637267130375	19.320290525769074	19.097072254014222
9	26.96863023283403	15.813926462506142	18.89806338838033	38.3193799162795
10-11	26.326329083047774	24.871206754053095	26.949163929598978	21.853300233300153
12-13	26.738767515243083	22.103436253261837	26.94849040077818	24.209305830716904
14-15	23.62836609670486	23.837978232539335	24.35420032334624	28.179455347409565
16-17	24.032697110606147	26.90459746180787	25.175404136214453	23.887301291371525
18-19	24.01862663814522	26.01062696875261	26.622931830543127	23.347814562559048
20-21	24.568278131473406	25.37574712500935	26.45410903989079	23.601865703626455
22-23	24.323889956206727	25.181078386033658	26.309393990849934	24.185637666909685
24-25	24.081361013946303	25.437302200215022	26.30757582640561	24.173760959433064
26-27	23.864782321551104	25.6423618131014	26.328387737612196	24.1644681277353
28-29	24.001599530255334	25.72565209550728	26.24998126260232	24.02276711163507
30-31	24.090813706630055	25.594675558702058	26.065949598946958	24.248561135720927
32-33	23.791892904304788	25.65450438349219	26.01798802059882	24.535614691604202
34-35	24.28809972435124	25.667816519244997	26.041138967550225	24.002944788853537
36-37	24.422904151189123	25.32515201146562	26.04771710843117	24.20422672891409
38-39	24.11801498111963	25.54001542303787	26.09776612305857	24.244203472783934
40-41	24.320149797605332	25.404226623628652	26.195722397491693	24.079901181274327
42-43	24.259694043047748	25.305821669919016	26.018577152121487	24.41590713491175
44-45	24.150023538908492	25.39700823360323	26.142700795703245	24.310267431785032
46-47	24.24056160155581	25.440363873363392	25.99027071530286	24.32880380977794
48-49	24.05555493089745	25.53234292271315	25.905030830929327	24.507071315460074
50-51	23.963800772975837	25.752992026075212	26.003023287579865	24.28018391336909
52-53	24.24708803394153	25.396290860760335	26.059042218471685	24.29757888682645
54-55	23.82176569197709	25.344173983958868	26.407323809526705	24.426736514537332
56-57	23.78962631289769	25.564550755498	26.243575626174586	24.40224730542972
58-59	23.805901934309315	25.262473244346644	26.21507708135618	24.716547739987856
60-61	23.66362765191844	25.319256136735145	26.509251934246635	24.50786427709978
62-63	23.6835459156926	25.327504679506603	26.456132683728566	24.53281672107223
64-65	23.50979845429836	25.28945037496203	26.36483411696845	24.835917053771166
66-67	23.56829593943984	25.31282219728282	26.333497568408472	24.78538429486887
68-69	23.801191836206183	25.122163135145335	26.31778357554205	24.758861453106423
70-71	23.587286478367147	25.06800593843334	26.361269480642292	24.983438102557223
72-73	23.678971257695306	25.123103626977635	26.289512391166063	24.908412724160996
74-75	23.69262753149563	25.287712754971874	26.210774725741988	24.80888498779051
76-77	23.65386948695922	25.05696462703564	26.424996435202612	24.86416945080253
78-79	23.66512957420661	25.137998933090692	26.33580431310159	24.861067179601108
80-81	23.75732118819412	25.18813762094778	26.287369517946374	24.767171672911722
82-83	23.79628839324795	25.169376951005297	26.287788791308458	24.746545864438296
84-85	23.653367899648337	25.23334920407111	26.273543176380088	24.83973971990046
86-87	23.760361450393503	25.1811412643718	26.211440098106927	24.84705718712778
88-89	23.68355367093365	25.049377893986673	26.144282654393937	25.122785780685735
90-91	23.62187024458451	25.06191960104138	26.308422265630877	25.007787888743234
92-93	23.951693038390403	24.917455636533564	26.277620905984094	24.853230419091947
94-95	23.749558406795508	24.844177971402075	26.378856745190017	25.027406876612403
96-97	24.050274357422836	24.569478769498453	26.33364985285791	25.046597020220794
98	24.034189539932598	24.564194232793223	26.202625268244823	25.198990959029356
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	70.0
1	54.0
2	84.5
3	156.0
4	174.5
5	185.5
6	291.5
7	510.5
8	815.0
9	1013.5
10	1024.5
11	1041.5
12	1155.5
13	1349.0
14	1526.5
15	1684.0
16	1839.5
17	1983.0
18	2028.0
19	1979.5
20	1951.5
21	2030.5
22	2225.0
23	2559.5
24	3130.5
25	3914.0
26	5117.0
27	6892.5
28	9362.0
29	12937.5
30	18115.0
31	25285.0
32	31218.0
33	36225.5
34	42319.0
35	49311.0
36	57904.0
37	66212.5
38	73481.0
39	80920.5
40	88587.0
41	96363.0
42	105165.5
43	113660.5
44	121856.0
45	129649.5
46	136425.5
47	142504.0
48	146069.5
49	147374.0
50	147790.0
51	147226.0
52	146751.0
53	146882.5
54	143762.0
55	138201.5
56	129048.0
57	117308.5
58	106673.5
59	93116.5
60	77889.0
61	64469.5
62	52638.5
63	40800.5
64	30018.0
65	22342.5
66	17067.5
67	13253.5
68	10106.5
69	7586.5
70	5757.0
71	4246.0
72	3188.5
73	2438.5
74	1959.5
75	1457.0
76	953.0
77	618.0
78	362.5
79	183.0
80	103.5
81	66.5
82	38.5
83	26.0
84	14.0
85	9.5
86	9.5
87	9.5
88	9.5
89	7.5
90	9.5
91	7.5
92	3.5
93	2.5
94	3.5
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4001721378749325
2	0.02681331877464949
3	0.01979222401650199
4	0.01715931348219668
5	0.022515924569231627
6	0.025390941819335128
7	0.017098786803247137
8	0.011711912376737419
9	0.0
10-11	3.026333947477369E-5
12-13	0.00623424793180338
14-15	0.00393423413172058
16-17	0.00738425483184478
18-19	0.039841686418539565
20-21	0.0859176207688825
22-23	0.10527102636300029
24-25	0.07196622127101183
26-27	0.08181693827005067
28-29	0.06381025128256032
30-31	0.057409554983645694
32-33	0.02241000288106992
34-35	0.3550494987180449
36-37	0.5441499754261683
38-39	0.49600100232180344
40-41	0.5051556625129225
42-43	0.45125665490835054
44-45	0.42462491617054965
46-47	0.42224924402177993
48-49	0.41276168709643835
50-51	0.4097504848186984
52-53	0.472516650889379
54-55	0.4738331061565316
56-57	0.442162521396181
58-59	0.4277874351456635
60-61	0.442162521396181
62-63	0.4211748954704254
64-65	0.47195677910909567
66-67	0.495047707128348
68-69	0.43465721320643713
70-71	0.39001878748114593
72-73	0.3748417227345469
74-75	0.35734951251812774
76-77	0.3545198902772364
78-79	0.3522501398166284
80-81	0.37092262027256373
82-83	0.3666403577368833
84-85	0.36635285601187295
86-87	0.3508731578705262
88-89	0.3973122522945664
90-91	0.37397921755951585
92-93	0.3633870487433451
94-95	0.3713614386949479
96-97	0.37139170203442273
98	0.3659745642684383
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3304328.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.80715804884489
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.22211969599918	37.93429927762069
2	12.58431626873425	13.039153237419306
3	5.2949792459207465	8.22953479976309
4	2.79878542724557	5.799884758964605
5	1.6417377177047547	4.252688270794007
6	1.057541588145363	3.2872934520163946
7	0.7080669230566071	2.567805449436706
8	0.506605615650967	2.0996637758769614
9	0.38497397249408766	1.794996669392358
>10	1.694236697171192	14.889145697083286
>50	0.07880796416947544	2.7622503158308165
>100	0.025770987688071068	2.258169606464997
>500	0.0011171435546462327	0.3876171135708971
>1k	9.4075246509575E-4	0.6974975757659959
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5131669737386845E-4
2	0.0	0.0	0.0	0.0	1.5131669737386845E-4
3	0.0	0.0	0.0	0.0	1.5131669737386845E-4
4	0.0	0.0	0.0	0.0	1.5131669737386845E-4
5	0.0	0.0	0.0	0.0	1.5131669737386845E-4
6	0.0	0.0	0.0	0.0	1.5131669737386845E-4
7	0.0	0.0	0.0	0.0	2.1184337632341584E-4
8	0.0	0.0	0.0	0.0	2.4210671579818954E-4
9	0.0	0.0	0.0	0.0	3.328967342225106E-4
10-11	0.0	0.0	0.0	3.0263339474773692E-5	3.4802840395989744E-4
12-13	0.0	0.0	0.0	3.0263339474773692E-5	3.93423413172058E-4
14-15	0.0	0.0	0.0	6.0526678949547384E-5	4.0855508290944483E-4
16-17	0.0	0.0	0.0	6.0526678949547384E-5	4.8421343159637907E-4
18-19	0.0	0.0	0.0	6.0526678949547384E-5	4.993451013337659E-4
20-21	0.0	0.0	0.0	1.0592168816170792E-4	5.144767710711527E-4
22-23	0.0	0.0	0.0	2.421067157981895E-4	5.598717802833132E-4
24-25	0.0	0.0	0.0	7.111884776571818E-4	6.657934684450212E-4
26-27	0.0	0.0	0.0	0.0015282986434760715	6.960568079197949E-4
28-29	0.0	3.0263339474773692E-5	0.0	0.006960568079197949	6.960568079197949E-4
30-31	0.0	3.0263339474773692E-5	0.0	0.02796332567469089	6.960568079197949E-4
32-33	0.0	3.0263339474773692E-5	0.0	0.07063463433412179	7.263201473945686E-4
34-35	0.0	3.0263339474773692E-5	0.0	0.12553233214136128	8.322418355562766E-4
36-37	0.0	4.5395009212160536E-5	0.0	0.21927302616447278	8.776368447684371E-4
38-39	0.0	6.0526678949547384E-5	0.0	0.3735101357976569	9.381635237179844E-4
40-41	0.0	6.0526678949547384E-5	0.0	0.549536849852678	9.684268631927581E-4
42-43	0.0	6.0526678949547384E-5	0.0	0.7327813703724327	9.684268631927581E-4
44-45	0.0	6.0526678949547384E-5	0.0	0.9570327158805059	9.684268631927581E-4
46-47	0.0	6.0526678949547384E-5	0.0	1.225453405352011	9.83558532930145E-4
48-49	0.0	6.0526678949547384E-5	0.0	1.5184781898165074	9.986902026675318E-4
50-51	6.0526678949547384E-5	6.0526678949547384E-5	0.0	1.8385281364319765	9.986902026675318E-4
52-53	6.0526678949547384E-5	6.0526678949547384E-5	0.0	2.156520175963161	0.0010138218724049185
54-55	6.0526678949547384E-5	6.0526678949547384E-5	0.0	2.511645333029893	0.0010592168816170792
56-57	6.0526678949547384E-5	6.0526678949547384E-5	0.0	2.9297636312133664	0.0010592168816170792
58-59	6.0526678949547384E-5	6.0526678949547384E-5	0.0	3.3782511905597747	0.0010743485513544661
60-61	6.0526678949547384E-5	6.0526678949547384E-5	0.0	3.856911299362533	0.0010894802210918528
62-63	6.0526678949547384E-5	6.0526678949547384E-5	0.0	4.341972104464206	0.0011500069000414002
64-65	6.0526678949547384E-5	6.0526678949547384E-5	0.0	4.858325202582794	0.0011500069000414002
66-67	6.0526678949547384E-5	6.0526678949547384E-5	0.0	5.449776777608034	0.0011500069000414002
68-69	6.0526678949547384E-5	6.0526678949547384E-5	0.0	6.095535915320754	0.0011500069000414002
70-71	6.0526678949547384E-5	6.0526678949547384E-5	0.0	6.779169622386155	0.0011651385697787871
72-73	6.0526678949547384E-5	6.0526678949547384E-5	0.0	7.483125161908866	0.0012256652487283345
74-75	6.0526678949547384E-5	6.0526678949547384E-5	0.0	8.195660358172676	0.001271060257940495
76-77	6.0526678949547384E-5	6.0526678949547384E-5	0.0	8.979057163816666	0.001271060257940495
78-79	6.0526678949547384E-5	6.0526678949547384E-5	0.0	9.829729373113082	0.001271060257940495
80-81	6.0526678949547384E-5	6.0526678949547384E-5	0.0	10.713070857372513	0.001271060257940495
82-83	6.0526678949547384E-5	6.0526678949547384E-5	0.0	11.617097334162953	0.001271060257940495
84-85	6.0526678949547384E-5	6.0526678949547384E-5	0.0	12.524452778295617	0.001271060257940495
86	6.0526678949547384E-5	6.0526678949547384E-5	0.0	13.217150355533713	0.001271060257940495
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4930	0.0	42.01234	1
GGTATCA	2610	0.0	41.794495	1
TCAACGC	6160	0.0	33.43043	4
CAACGCA	6365	0.0	32.353725	5
ATCAACG	6440	0.0	32.048153	3
TATCAAC	6660	0.0	30.922043	2
AACGCAG	6735	0.0	30.70786	6
GTACATG	10370	0.0	26.40885	1
ACGCAGA	7875	0.0	25.55756	7
TACATGG	10415	0.0	25.322388	2
ACATGGG	10730	0.0	23.893976	3
CGCAGAG	8430	0.0	23.547922	8
GAGTACA	7540	0.0	21.54838	1
GCAGAGT	9265	0.0	20.633974	9
CATGGGG	7700	0.0	19.715763	4
AGTACAT	7790	0.0	19.606627	2
AGAGTAC	7850	0.0	17.870935	10-11
GTCTTAG	1165	0.0	16.988451	1
TAAGGTG	1525	0.0	16.842033	5
CAGAGTA	8580	0.0	16.537464	10-11
>>END_MODULE