FastQCFastQC Report
Mon 15 Aug 2016
SRR2920857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304328
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48150.047.3122371
GGTATCA24100.045.78311
TCAACGC62650.035.8385774
ATCAACG63500.035.358853
CAACGCA65000.034.391085
TATCAAC67050.033.6976132
AACGCAG68850.032.6747786
GTACATG102000.026.6704371
ACGCAGA83350.026.3637457
TACATGG102500.025.6552832
CGCAGAG87150.024.7766638
ACATGGG110000.023.6913783
GCAGAGT97750.020.973259
CATGGGG81050.020.5572384
GAGTACA75000.020.1016751
AGAGTAC83700.018.65275210-11
AGTACAT77800.017.9983792
CGTACAC4306.91216E-1116.5589773
CAGAGTA91750.015.9817710-11
GGACCGT5103.6379788E-1215.8203846
Produced by FastQC (version 0.11.5)