##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920857_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3304328
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.272285620555827	31.0	30.0	34.0	27.0	34.0
2	30.289755435901036	31.0	30.0	34.0	26.0	34.0
3	30.429188627763345	31.0	30.0	34.0	27.0	34.0
4	34.07243590829966	35.0	35.0	37.0	30.0	37.0
5	33.59159108902022	35.0	35.0	37.0	28.0	37.0
6	33.53761642306696	35.0	35.0	37.0	28.0	37.0
7	33.34703879275907	35.0	35.0	37.0	27.0	37.0
8	33.27752087565157	35.0	33.0	37.0	27.0	37.0
9	34.619652467914804	38.0	35.0	39.0	27.0	39.0
10-11	34.42817193087369	38.0	34.0	39.0	27.0	39.0
12-13	34.24317380114807	37.0	34.0	39.0	26.5	39.0
14-15	35.15338625584385	38.0	34.0	40.0	26.0	41.0
16-17	35.02432975782065	38.0	34.0	40.0	25.5	41.0
18-19	34.92621858362729	38.5	34.0	40.0	25.0	41.0
20-21	34.80588352609063	38.0	34.0	40.0	25.0	41.0
22-23	34.653487184081	38.0	34.0	40.0	24.5	41.0
24-25	34.46646882512874	38.0	33.0	40.0	24.0	41.0
26-27	34.27440511353595	38.0	33.0	40.0	23.0	41.0
28-29	34.216016993470376	38.0	33.0	40.0	23.0	41.0
30-31	34.20896684590634	38.0	33.0	40.0	23.0	41.0
32-33	34.0605238039323	38.0	33.0	40.0	22.0	41.0
34-35	33.89068806123363	38.0	33.0	40.0	21.0	41.0
36-37	33.6896606511218	38.0	33.0	40.0	19.0	41.0
38-39	33.49790396110798	38.0	32.0	40.0	18.0	41.0
40-41	33.25866999280943	38.0	32.0	40.0	17.0	41.0
42-43	33.280575808454856	38.0	32.0	40.0	16.0	41.0
44-45	33.12791617539179	38.0	32.0	40.0	15.0	41.0
46-47	32.873439924850075	37.5	31.5	40.0	13.5	41.0
48-49	32.595688442551705	37.0	31.0	40.0	10.0	41.0
50-51	32.32720283821703	37.0	31.0	40.0	8.5	41.0
52-53	32.05657761578148	37.0	31.0	40.0	8.0	41.0
54-55	31.763628943615767	36.0	30.0	39.5	7.0	41.0
56-57	31.428685953694668	36.0	30.0	39.0	2.0	41.0
58-59	31.083497764144482	35.0	29.0	39.0	2.0	40.0
60-61	30.738041290089846	35.0	29.0	39.0	2.0	40.0
62-63	30.40721123932007	35.0	28.5	38.5	2.0	40.0
64-65	29.996554216167404	34.5	28.0	38.0	2.0	40.0
66-67	29.556148178994338	34.0	28.0	37.5	2.0	40.0
68-69	29.10435268532664	34.0	27.5	37.0	2.0	39.5
70-71	28.668671209395676	34.0	26.0	36.0	2.0	39.0
72-73	28.225456583002654	33.5	26.0	36.0	2.0	39.0
74-75	27.78370337327287	33.0	26.0	35.5	2.0	38.0
76-77	26.3509094738779	31.5	24.0	34.0	2.0	36.0
78-79	27.19765077800993	33.0	26.0	35.0	2.0	37.0
80-81	27.22611042245201	33.0	26.0	35.0	2.0	36.5
82-83	26.896740275178495	33.0	25.0	35.0	2.0	36.0
84-85	26.581166730421437	33.0	25.0	35.0	2.0	36.0
86-87	26.048289092366133	32.0	23.5	35.0	2.0	35.0
88-89	25.685435132347635	32.0	22.0	35.0	2.0	35.0
90-91	25.315635281969584	32.0	20.0	34.5	2.0	35.0
92-93	25.00293917552979	32.0	19.0	34.0	2.0	35.0
94-95	24.618278966252745	32.0	16.5	34.0	2.0	35.0
96-97	24.08529707099295	31.0	4.5	34.0	2.0	35.0
98-99	23.48286050900516	31.0	2.0	34.0	2.0	35.0
100-101	21.281945073249386	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76226.0
3	35341.0
4	19271.0
5	13651.0
6	11922.0
7	11882.0
8	13293.0
9	15336.0
10	17421.0
11	19385.0
12	21362.0
13	23578.0
14	25617.0
15	27303.0
16	28403.0
17	29707.0
18	28899.0
19	27961.0
20	28065.0
21	29160.0
22	31550.0
23	35729.0
24	41043.0
25	48607.0
26	57623.0
27	68413.0
28	80119.0
29	94364.0
30	110740.0
31	131844.0
32	158313.0
33	192982.0
34	243206.0
35	315648.0
36	421327.0
37	482183.0
38	268780.0
39	18073.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08742716222481	18.56150757252091	13.289533889786595	25.061531375467684
2	16.403456315474735	20.00306870262274	37.64689824981055	25.94657673209197
3	19.9052092536447	23.28466068526706	28.789685188060567	28.020444873027678
4	14.084132321307122	15.64385007023588	35.0302539418203	35.2417636666367
5	14.775288142179802	36.68035875449215	33.73795208253508	14.806401020792967
6	34.975593470102	34.38448646310118	16.2713026955037	14.368617371293121
7	30.314264161556626	31.02657363172967	19.902022973442733	18.757139233270973
8	27.410594242505876	34.29694580641916	19.25457350284881	19.037886448226157
9	26.768739799884532	15.67149907791714	18.795033935983053	38.764727186215275
10-11	26.362231745092483	24.93005932024037	26.959569916516575	21.748139018150567
12-13	26.64981348077119	22.1298278060297	27.03662272941017	24.183735983788946
14-15	23.566536233910586	23.87201218267898	24.336225335930372	28.225226247480055
16-17	24.01583971794739	27.003820926149857	25.163919458014178	23.816419897888572
18-19	24.020211745995063	26.075732865849126	26.621597207088772	23.282458181067042
20-21	24.53070046238928	25.327930667947097	26.45544541865863	23.685923451004992
22-23	24.274142362371578	25.20145611475665	26.364024751588317	24.160376771283456
24-25	24.08048833701274	25.4877412323766	26.33414299909626	24.097627431514404
26-27	23.860177492304175	25.657237861188964	26.312121170825364	24.170463475681494
28-29	23.990280877258154	25.728457362503416	26.304377904390986	23.976883855847444
30-31	24.060423269836452	25.58541399556924	26.12994326766905	24.224219466925263
32-33	23.782882622560145	25.59575120892468	26.07814042280539	24.543225745709787
34-35	24.218225372844966	25.655211429780465	26.08035783067123	24.046205366703337
36-37	24.351125777837044	25.369463608207305	26.130345002219634	24.149065611736017
38-39	24.10549334910792	25.513369067331155	26.181917039019847	24.19922054454108
40-41	24.24916518411357	25.388574215291232	26.281662990177157	24.080597610418042
42-43	24.205659780003717	25.313238337781645	26.103507579284198	24.37759430293044
44-45	24.080422713430323	25.369389905007537	26.189040448989232	24.361146932572908
46-47	24.17053677102439	25.405867119075698	26.081877134195857	24.341718975704055
48-49	23.981384419189247	25.49808845715043	26.028020828651517	24.492506295008802
50-51	23.915919958968217	25.705975321302372	26.058904337016337	24.319200382713078
52-53	24.195031154592606	25.389506179845416	26.04228852898115	24.373174136580825
54-55	23.76063706625059	25.379045036237528	26.320418778441805	24.539899119070068
56-57	23.732288876476975	25.568678521270144	26.116879152986368	24.58215344926651
58-59	23.701474785198275	25.355395086874015	26.11321616967445	24.829913958253258
60-61	23.611406458416926	25.42981420438834	26.33033045213943	24.628448885055302
62-63	23.55957145044979	25.45407329700949	26.26061522126937	24.725740031271357
64-65	23.36844851469956	25.361061282130358	26.182833400775223	25.087656802394857
66-67	23.403884601242307	25.382607048910156	26.15205219432422	25.06145615552332
68-69	23.656400915881346	25.22278338146082	26.082277865605107	25.038537837052726
70-71	23.425970461982917	25.122843001604007	26.102756774640568	25.34842976177251
72-73	23.43851026984835	25.129651376052347	26.042119722980463	25.38971863111884
74-75	23.315718236600237	25.267918033688137	25.929040738262017	25.487322991449613
76-77	23.268833928394255	24.915641125925855	26.270890218618515	25.544634727061382
78-79	23.222348975817322	24.9885247865368	26.18006915567113	25.609057081974747
80-81	23.204485192629004	25.08933760321573	26.081495023851613	25.624682180303655
82-83	23.161061777621867	24.996254461730587	26.11104771861156	25.731636042035987
84-85	22.952531265791045	24.949555682913825	26.09907925217255	25.99883379912258
86-87	22.897370610369258	24.921323754665156	26.091608721914806	26.08969691305078
88-89	22.79797164437903	24.775817854262154	25.963801639477314	26.462408861881503
90-91	22.656803097932578	24.759912349458737	26.115986149005316	26.467298403603372
92-93	22.788276464777535	24.60850556891461	26.12721072888281	26.476007237425048
94-95	22.4281735903006	24.466813708014378	26.297375590369597	26.807637111315426
96-97	22.556466070420917	24.206681578106725	26.324018298670364	26.912834052801994
98-99	22.366084122061835	24.274119339551763	26.23245401586854	27.12734252251786
100-101	22.507723236139977	24.051417118307945	26.326523972486136	27.114335673065938
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	45.5
2	60.0
3	106.0
4	106.5
5	93.0
6	161.5
7	342.5
8	523.0
9	573.5
10	503.0
11	449.5
12	463.5
13	503.0
14	558.5
15	618.5
16	680.0
17	722.0
18	813.5
19	941.0
20	1086.0
21	1308.0
22	1589.5
23	1914.5
24	2399.0
25	3069.0
26	3978.0
27	5430.5
28	7415.0
29	10158.5
30	14123.5
31	19647.5
32	27109.0
33	32591.5
34	36470.0
35	42457.5
36	50141.5
37	58429.5
38	66115.5
39	72761.0
40	79497.5
41	87352.0
42	95124.5
43	103466.5
44	111957.0
45	120670.0
46	128308.0
47	134030.5
48	139558.0
49	144153.5
50	145976.5
51	145820.0
52	146066.5
53	147664.5
54	146673.5
55	140071.0
56	130530.5
57	119412.0
58	108264.5
59	95493.0
60	80005.0
61	66082.5
62	53979.0
63	41654.0
64	30927.0
65	23115.5
66	17705.5
67	13659.0
68	10448.0
69	8006.5
70	6063.0
71	4489.0
72	3252.5
73	2506.5
74	1980.0
75	1448.5
76	1007.0
77	637.0
78	376.0
79	207.5
80	106.0
81	43.5
82	22.5
83	12.5
84	7.5
85	5.5
86	3.5
87	1.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1941998494096228
2	0.0
3	0.012680339239930175
4	0.01395139949787067
5	0.00696056807919795
6	0.10810064860389161
7	0.14462849934994348
8	0.12640996898612972
9	0.14323638573410388
10-11	0.11658951532656564
12-13	0.11693754373052555
14-15	0.11816320897925388
16-17	0.06806225047876603
18-19	0.08121167148055519
20-21	0.03834365111453827
22-23	0.08481300887805328
24-25	0.058604956892899254
26-27	0.06697277025767417
28-29	0.04100682498831835
30-31	0.025194230112749096
32-33	0.032760064981442516
34-35	0.019444195612542098
36-37	0.025920550260143664
38-39	0.034031125239383016
40-41	0.02738832222467019
42-43	0.013739556121547257
44-45	0.027479112243094513
46-47	0.03397059856043347
48-49	0.009729663641139741
50-51	0.016569178362438597
52-53	0.007278333143683072
54-55	0.013104025992577008
56-57	0.004675685948852535
58-59	0.003571074058023296
60-61	0.023560009781111318
62-63	0.010471115458271696
64-65	0.0075355715292186485
66-67	0.04126406337385393
68-69	0.09160712859013996
70-71	0.02181986776131183
72-73	0.046257514387191584
74-75	0.08771828946763155
76-77	0.2598712960698817
78-79	0.08009192791998858
80-81	0.017189576821671455
82-83	0.012014545771485155
84-85	0.01324021102021349
86-87	0.2729601903927213
88-89	0.6289175892950094
90-91	0.27143189174924526
92-93	0.35470147031408505
94-95	0.001271060257940495
96-97	0.0046454226093777615
98-99	0.0035559423882859085
100-101	0.005311216077822783
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3304328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.515322909942235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.13964648862259	35.22502567583285
2	13.41672637122823	13.286670773314066
3	5.753354826029847	8.546376660790274
4	2.968961808761222	5.880364106723923
5	1.8283705112250577	4.526617813116245
6	1.1794068896829224	3.503922779091631
7	0.7756207454318506	2.6883578165995727
8	0.5411258342020768	2.1435216332342155
9	0.4168340202678148	1.857570400206923
>10	1.8594704756765785	15.745463988679893
>50	0.08827619158525739	2.969588290467209
>100	0.02993575510731311	2.4866538638620375
>500	0.0012884250221852195	0.4522887976678875
>1k	9.816571570888098E-4	0.687577400413382
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.0526678949547384E-5
2	0.0	0.0	0.0	0.0	6.0526678949547384E-5
3	0.0	0.0	0.0	0.0	6.0526678949547384E-5
4	0.0	0.0	0.0	0.0	6.0526678949547384E-5
5	3.0263339474773692E-5	0.0	0.0	0.0	6.0526678949547384E-5
6	3.0263339474773692E-5	0.0	0.0	0.0	9.079001842432107E-5
7	3.0263339474773692E-5	0.0	0.0	0.0	9.079001842432107E-5
8	3.0263339474773692E-5	0.0	0.0	0.0	9.079001842432107E-5
9	3.0263339474773692E-5	0.0	0.0	0.0	1.2105335789909477E-4
10-11	3.0263339474773692E-5	0.0	0.0	0.0	1.361850276364816E-4
12-13	3.0263339474773692E-5	0.0	0.0	0.0	1.5131669737386845E-4
14-15	3.0263339474773692E-5	0.0	0.0	3.0263339474773692E-5	1.8158003684864215E-4
16-17	3.0263339474773692E-5	0.0	0.0	3.0263339474773692E-5	2.1184337632341584E-4
18-19	3.0263339474773692E-5	0.0	0.0	3.0263339474773692E-5	2.269750460608027E-4
20-21	3.0263339474773692E-5	0.0	0.0	4.5395009212160536E-5	2.4210671579818954E-4
22-23	3.0263339474773692E-5	0.0	0.0	1.8158003684864215E-4	2.8750172501035005E-4
24-25	3.0263339474773692E-5	0.0	0.0	5.90135119758087E-4	3.026333947477369E-4
26-27	3.0263339474773692E-5	0.0	0.0	0.0013013235974152688	3.026333947477369E-4
28-29	3.0263339474773692E-5	0.0	0.0	0.006642803014712826	3.026333947477369E-4
30-31	3.0263339474773692E-5	0.0	0.0	0.027282400536508482	3.026333947477369E-4
32-33	3.0263339474773692E-5	0.0	0.0	0.06966620747092904	3.93423413172058E-4
34-35	3.0263339474773692E-5	0.0	0.0	0.12473035364527976	4.3881842238421853E-4
36-37	3.0263339474773692E-5	0.0	0.0	0.21662498396043006	4.8421343159637907E-4
38-39	3.0263339474773692E-5	0.0	0.0	0.3684107630961575	5.144767710711527E-4
40-41	3.0263339474773692E-5	0.0	0.0	0.5441651070959057	5.447401105459264E-4
42-43	3.0263339474773692E-5	0.0	0.0	0.7272885742577613	5.447401105459264E-4
44-45	3.0263339474773692E-5	0.0	0.0	0.950934652976339	5.90135119758087E-4
46-47	3.0263339474773692E-5	0.0	0.0	1.2175244104096203	6.203984592328606E-4
48-49	3.0263339474773692E-5	0.0	0.0	1.5083097077529835	6.960568079197949E-4
50-51	3.0263339474773692E-5	0.0	0.0	1.8233813350248522	6.960568079197949E-4
52-53	3.0263339474773692E-5	0.0	0.0	2.1365161085703352	7.565834868693423E-4
54-55	3.0263339474773692E-5	0.0	0.0	2.4864813662566183	8.322418355562766E-4
56-57	3.0263339474773692E-5	0.0	0.0	2.897170014599035	8.473735052936634E-4
58-59	3.0263339474773692E-5	0.0	0.0	3.339045034270206	8.473735052936634E-4
60-61	3.0263339474773692E-5	0.0	0.0	3.8119853719122316	8.776368447684371E-4
62-63	3.0263339474773692E-5	0.0	0.0	4.290721139063677	8.776368447684371E-4
64-65	3.0263339474773692E-5	0.0	0.0	4.798358395413531	8.776368447684371E-4
66-67	3.0263339474773692E-5	0.0	0.0	5.37764410796991	8.92768514505824E-4
68-69	3.0263339474773692E-5	0.0	0.0	6.009103817780801	9.079001842432108E-4
70-71	3.0263339474773692E-5	0.0	0.0	6.679739420541786	9.079001842432108E-4
72-73	3.0263339474773692E-5	0.0	0.0	7.370273169007435	9.986902026675318E-4
74-75	3.0263339474773692E-5	0.0	0.0	8.075666217155199	0.0010289535421423054
76-77	3.0263339474773692E-5	0.0	0.0	8.851920874683142	0.0010289535421423054
78-79	3.0263339474773692E-5	0.0	0.0	9.69371079384371	0.0010289535421423054
80-81	3.0263339474773692E-5	0.0	0.0	10.567201561104103	0.0010743485513544661
82-83	3.0263339474773692E-5	0.0	0.0	11.460635869078372	0.0011500069000414002
84-85	3.0263339474773692E-5	0.0	0.0	12.361182061829213	0.001195401909253561
86-87	3.0263339474773692E-5	0.0	0.0	13.285772477792761	0.0012407969184657214
88-89	3.0263339474773692E-5	0.0	0.0	14.26102372403708	0.0012407969184657214
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4815	0.0	47.312237	1
GGTATCA	2410	0.0	45.7831	1
TCAACGC	6265	0.0	35.838577	4
ATCAACG	6350	0.0	35.35885	3
CAACGCA	6500	0.0	34.39108	5
TATCAAC	6705	0.0	33.697613	2
AACGCAG	6885	0.0	32.674778	6
GTACATG	10200	0.0	26.670437	1
ACGCAGA	8335	0.0	26.363745	7
TACATGG	10250	0.0	25.655283	2
CGCAGAG	8715	0.0	24.776663	8
ACATGGG	11000	0.0	23.691378	3
GCAGAGT	9775	0.0	20.97325	9
CATGGGG	8105	0.0	20.557238	4
GAGTACA	7500	0.0	20.101675	1
AGAGTAC	8370	0.0	18.652752	10-11
AGTACAT	7780	0.0	17.998379	2
CGTACAC	430	6.91216E-11	16.558977	3
CAGAGTA	9175	0.0	15.98177	10-11
GGACCGT	510	3.6379788E-12	15.820384	6
>>END_MODULE