FastQCFastQC Report
Mon 15 Aug 2016
SRR2920856_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920856_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3556680
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33100.047.5536771
GTATCAA60800.041.8607981
TCAACGC78600.031.9088064
ATCAACG79100.031.707113
TATCAAC81550.030.9807362
CAACGCA81500.030.6046265
AACGCAG86450.028.7931356
GTACATG113650.026.2011781
TACATGG115650.025.2159652
ACGCAGA100600.024.284467
ACATGGG123750.023.0828083
CGCAGAG105850.023.0357118
TATAGCG1453.0694086E-522.1348115
CATGGGG84000.021.5607364
TAAGGCG11450.021.2234615
GCAGAGT122450.019.014549
GAGTACA88100.018.8066981
AGTACAT87600.017.9013632
AGAGTAC101250.017.65479710-11
GTATTAG8450.017.4292721
Produced by FastQC (version 0.11.5)