##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920856_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3556680
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.80103129885174	31.0	30.0	33.0	23.0	34.0
2	28.83494326169349	31.0	29.0	34.0	22.0	34.0
3	28.907244958781785	31.0	28.0	34.0	22.0	34.0
4	32.416325055951056	35.0	33.0	37.0	25.0	37.0
5	32.39327884431548	35.0	33.0	37.0	25.0	37.0
6	32.52072044715859	35.0	33.0	37.0	25.0	37.0
7	32.361014485418984	35.0	32.0	37.0	24.0	37.0
8	32.30945263560399	35.0	32.0	37.0	23.0	37.0
9	33.59427246758213	37.0	33.0	39.0	23.0	39.0
10-11	33.3993664034999	37.0	33.0	39.0	22.5	39.0
12-13	33.23726410585152	37.0	33.0	39.0	22.0	39.0
14-15	34.00660320861028	38.0	33.0	40.0	19.0	41.0
16-17	33.90485480841684	38.0	33.0	40.0	19.0	41.0
18-19	33.80716876412835	38.0	32.0	40.0	18.0	41.0
20-21	33.71692378847689	38.0	32.0	40.0	18.0	41.0
22-23	33.497832669793176	38.0	32.0	40.0	18.0	41.0
24-25	33.308352874028586	38.0	32.0	40.0	17.0	41.0
26-27	33.347550805807664	38.0	32.0	40.0	16.5	41.0
28-29	33.19409519551942	38.0	32.0	40.0	16.0	41.0
30-31	32.9448020344816	38.0	31.5	40.0	12.5	41.0
32-33	32.70167375192595	38.0	31.0	40.0	9.5	41.0
34-35	32.36828348347335	37.0	30.5	40.0	8.5	41.0
36-37	32.06409165288977	37.0	30.0	40.0	8.0	41.0
38-39	31.868931559769223	37.0	30.0	40.0	7.5	41.0
40-41	31.677906643274063	37.0	30.0	40.0	4.0	41.0
42-43	31.44253714137904	37.0	30.0	40.0	2.0	41.0
44-45	31.16656080951899	36.0	30.0	40.0	2.0	41.0
46-47	30.91404034099216	36.0	29.0	39.0	2.0	41.0
48-49	30.61519886523387	36.0	28.5	39.0	2.0	40.0
50-51	29.464731997255868	34.5	27.0	38.0	2.0	39.5
52-53	29.90239043152603	35.0	28.0	38.5	2.0	39.5
54-55	30.565425059324987	36.0	29.0	39.0	2.0	40.0
56-57	30.519010847194576	36.0	28.5	39.0	2.0	41.0
58-59	30.28612877739915	35.5	28.0	39.0	2.0	41.0
60-61	29.961506095572275	35.0	28.0	39.0	2.0	40.0
62-63	29.58085152445539	35.0	28.0	39.0	2.0	40.0
64-65	29.17001318645478	34.5	26.5	38.0	2.0	40.0
66-67	28.786667200872724	34.0	26.0	38.0	2.0	40.0
68-69	28.339370564683918	34.0	26.0	37.0	2.0	40.0
70-71	28.01014049619308	34.0	26.0	37.0	2.0	39.0
72-73	27.545439004914698	33.5	25.0	36.0	2.0	39.0
74-75	27.099537771179865	33.0	24.5	36.0	2.0	39.0
76-77	26.67051857350113	33.0	23.5	35.0	2.0	37.5
78-79	26.199304829222758	33.0	22.0	35.0	2.0	37.0
80-81	25.7357133618993	32.0	20.0	35.0	2.0	36.5
82-83	25.242880298480607	32.0	18.5	35.0	2.0	36.0
84-85	24.828332321153436	32.0	16.5	35.0	2.0	35.5
86-87	24.34869021109574	31.5	8.5	34.0	2.0	35.0
88-89	23.792441406030342	31.0	2.0	34.0	2.0	35.0
90-91	23.281545992329924	31.0	2.0	34.0	2.0	35.0
92-93	22.72127335042793	31.0	2.0	34.0	2.0	35.0
94-95	22.067428332039995	30.0	2.0	34.0	2.0	35.0
96-97	21.416939814658615	29.5	2.0	34.0	2.0	35.0
98	21.01654604856214	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	158882.0
3	28881.0
4	21153.0
5	17592.0
6	16365.0
7	16961.0
8	19320.0
9	22384.0
10	24388.0
11	26586.0
12	27929.0
13	27990.0
14	26393.0
15	27992.0
16	30072.0
17	32575.0
18	33377.0
19	32607.0
20	33897.0
21	35986.0
22	38753.0
23	43742.0
24	49825.0
25	57390.0
26	66336.0
27	76809.0
28	89018.0
29	103094.0
30	119809.0
31	139784.0
32	166745.0
33	201919.0
34	249099.0
35	316416.0
36	403257.0
37	455871.0
38	284994.0
39	32487.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.14994939292687	18.239099716402762	13.114078365440946	24.496872525229428
2	15.978063391174732	19.801670557133615	39.00734032679922	25.212925724892425
3	18.94699810206338	23.984080842145048	29.496111746818798	27.57280930897278
4	13.256393708160916	15.809180730785833	36.67452654431129	34.25989901674196
5	14.209899176873142	37.0087892253597	34.37815239250684	14.40315920526032
6	33.23621686744278	35.24177500402152	17.292486672733634	14.229521455802072
7	28.845799091586034	31.836051301317625	21.202849873231482	18.115299733864852
8	26.628285801535647	34.171154963352215	20.29443076288015	18.906128472231988
9	26.846694107988352	15.528582835678218	19.774087069964125	37.85063598636931
10-11	25.435525668748856	25.337203610446714	27.968436285647368	21.258834435157066
12-13	26.093420581286864	22.84647854295318	27.661119755801707	23.39898111995825
14-15	23.308759872989164	24.46871923806996	25.22474748681797	26.997773402122903
16-17	23.3261074917857	27.321863370157057	26.04369218336776	23.308336954689487
18-19	23.302085431121483	26.544904948398628	27.514912812643093	22.638096807836796
20-21	24.131704992500197	25.931094681253843	27.079638596917267	22.857561729328694
22-23	23.88364772572639	25.744742618712802	26.859297178946917	23.512312476613886
24-25	23.522104911320948	25.868122864896893	27.02918848778852	23.580583735993642
26-27	23.343572303765534	26.087215881232634	26.94162802021607	23.627583794785764
28-29	23.431146796153104	26.248996006532465	26.870466204630393	23.449390992684034
30-31	23.624501333294322	26.027103613243092	26.66443771100413	23.683957342458456
32-33	23.413691228209306	26.182367946045794	26.507981302647472	23.895959523097428
34-35	23.85910551298772	26.084868418453638	26.560828988291384	23.495197080267253
36-37	23.972903277973376	25.753223111008577	26.614085873668692	23.659787737349355
38-39	23.660889641198963	25.937172375400213	26.585342764212022	23.816595219188805
40-41	23.821987790197593	25.808698399680193	26.73150601269816	23.637807797424053
42-43	23.72324947030682	25.798875618352003	26.51080133447939	23.967073576861793
44-45	23.619719737726065	25.830915826716666	26.66090682125154	23.88845761430573
46-47	23.71090410417336	25.804664362841823	26.466471582826507	24.017959950158314
48-49	23.427045732795424	25.873188962326804	26.492058992279233	24.207706312598543
50-51	23.411189363840464	26.012404391696204	26.581575470402242	23.99483077406109
52-53	23.579056810604946	25.846353136618937	26.640339823928937	23.934250228847176
54-55	23.21363059810024	25.783197580074972	26.80823015223982	24.194941669584967
56-57	23.188042717974405	25.848757989708155	26.737498942613907	24.22570034970353
58-59	23.19829147325132	25.595340381594507	26.83835713151067	24.368011013643503
60-61	23.06827385235235	25.548095179925763	27.032875472294972	24.35075549542692
62-63	23.078659734906925	25.527403968814138	27.0798914913501	24.314044804928834
64-65	22.939819932320997	25.53452074126237	26.85182451488592	24.673834811530718
66-67	22.925974745842723	25.586595320406097	26.80824296010023	24.679186973650953
68-69	23.200296426250556	25.29648979467342	26.82639210335832	24.676821675717704
70-71	22.977139856764182	25.29828563040416	26.845113919619696	24.879460593211967
72-73	23.02784727809414	25.331831571406816	26.773963331963945	24.866357818535093
74-75	23.05856812158767	25.568231363566195	26.69494407214393	24.678256442702203
76-77	23.094299795831695	25.337371548222244	26.807143492082304	24.76118516386376
78-79	23.078998282008044	25.449138633598146	26.690181822696907	24.781681261696907
80-81	23.213134075943337	25.43251100946095	26.65659823955876	24.697756675036953
82-83	23.219286400799717	25.36382066984293	26.70229013450453	24.714602794852823
84-85	23.153240236392474	25.354377042610476	26.709301603607976	24.783081117389074
86-87	23.22680948670205	25.362647955469903	26.706418224369283	24.704124333458765
88-89	23.242725770306382	25.201529461385448	26.588775442794542	24.966969325513627
90-91	23.247331779470382	25.226827675319036	26.63422333289491	24.891617212315673
92-93	23.527558241790494	25.046721560384405	26.610743785792586	24.814976412032514
94-95	23.406550496331615	24.968469913375348	26.723669431755653	24.90131015853738
96-97	23.69137534413909	24.722977646147186	26.676263776676823	24.9093832330369
98	23.73494842989528	24.69678133118464	26.502955632352922	25.065314606567163
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	110.0
1	102.5
2	163.5
3	261.0
4	268.5
5	301.0
6	440.0
7	786.0
8	1194.5
9	1362.5
10	1339.5
11	1367.5
12	1537.0
13	1710.0
14	1895.5
15	2087.0
16	2206.5
17	2262.5
18	2342.5
19	2463.5
20	2529.5
21	2623.5
22	2864.0
23	3265.0
24	3892.5
25	4932.0
26	6560.0
27	8983.0
28	12146.0
29	16512.0
30	22797.5
31	31151.0
32	39002.5
33	46330.0
34	54965.0
35	64288.0
36	74043.0
37	83060.5
38	90814.0
39	98472.0
40	105802.5
41	112597.0
42	121047.0
43	130679.0
44	138424.5
45	144508.0
46	150288.0
47	155254.0
48	158023.5
49	157962.5
50	157358.0
51	156191.0
52	152989.5
53	150094.5
54	145588.5
55	137148.0
56	125340.0
57	111721.5
58	99880.5
59	86667.5
60	71750.0
61	58808.5
62	47886.5
63	36964.0
64	27070.0
65	20269.0
66	15207.5
67	11488.0
68	8696.5
69	6528.0
70	4798.0
71	3567.5
72	2842.0
73	2272.0
74	1711.0
75	1233.5
76	811.5
77	469.5
78	272.0
79	148.0
80	81.5
81	64.5
82	47.5
83	30.5
84	20.5
85	13.0
86	10.0
87	5.0
88	4.0
89	5.0
90	5.0
91	4.0
92	3.5
93	2.0
94	1.5
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.412885050102905
2	0.027553786115141088
3	0.020046785204179178
4	0.018134889841087756
5	0.02387057593036202
6	0.022830279923974045
7	0.016644736102207677
8	0.011077746662617946
9	0.0
10-11	2.8116108280756215E-5
12-13	0.006354240471450904
14-15	0.003739442401340576
16-17	0.006508879066995063
18-19	0.03790051396245937
20-21	0.08208497812566776
22-23	0.09999493910050947
24-25	0.06753489209037641
26-27	0.07800814242495811
28-29	0.05974673009660695
30-31	0.05485452725575537
32-33	0.021804041971726442
34-35	0.35971748934399495
36-37	0.5523971793920173
38-39	0.5046841436395739
40-41	0.5143420268340138
42-43	0.4617508462948593
44-45	0.43571533002687896
46-47	0.43488590483259665
48-49	0.4235410551413116
50-51	0.4203498768514457
52-53	0.48359706242900685
54-55	0.48448271983985064
56-57	0.4529364463488421
58-59	0.4391033210747101
60-61	0.45330195575649196
62-63	0.43286154503638224
64-65	0.4829644499926898
66-67	0.5054011044007333
68-69	0.44516234240921315
70-71	0.4012168651663911
72-73	0.38604822474892314
74-75	0.36823667015306405
76-77	0.3664934714396572
78-79	0.3657343365160768
80-81	0.3821260276437577
82-83	0.3772197667487657
84-85	0.37731817312774835
86-87	0.36324606093322986
88-89	0.4081896600200187
90-91	0.3846845934973065
92-93	0.37529381333153394
94-95	0.3833771944622513
96-97	0.38236501456414407
98	0.37802107583476724
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3556680.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.61071637549134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.92745298807986	43.74234959209723
2	12.543738558574713	14.453075287727248
3	4.72542692003768	8.167056901302072
4	2.3391691067256035	5.390448318475208
5	1.3155300844786162	3.7894315290161864
6	0.75790111876422	2.6197935836275836
7	0.513219079441094	2.0696843176920137
8	0.3493788499794552	1.6102372667009386
9	0.26749605978909496	1.3869575668863923
>10	1.1852213538574092	11.729096106119815
>50	0.05240836292499274	2.054730309814722
>100	0.021487203686634957	2.092025841699887
>500	9.81446044296696E-4	0.38397542813681373
>1k	5.888676162712983E-4	0.5111379507040094
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.811610828075621E-5
2	0.0	0.0	0.0	0.0	2.811610828075621E-5
3	0.0	0.0	0.0	0.0	2.811610828075621E-5
4	0.0	0.0	0.0	0.0	5.623221656151242E-5
5	0.0	0.0	0.0	2.811610828075621E-5	5.623221656151242E-5
6	0.0	0.0	0.0	2.811610828075621E-5	1.4058054140378106E-4
7	0.0	0.0	0.0	2.811610828075621E-5	1.4058054140378106E-4
8	0.0	0.0	0.0	2.811610828075621E-5	1.4058054140378106E-4
9	0.0	0.0	0.0	5.623221656151242E-5	2.249288662460497E-4
10-11	0.0	0.0	0.0	5.623221656151242E-5	2.811610828075621E-4
12-13	0.0	0.0	0.0	5.623221656151242E-5	7.310188152996615E-4
14-15	0.0	0.0	0.0	5.623221656151242E-5	8.575413025630644E-4
16-17	0.0	0.0	0.0	5.623221656151242E-5	8.997154649841988E-4
18-19	0.0	0.0	0.0	8.434832484226863E-5	9.27831573264955E-4
20-21	0.0	0.0	0.0	1.2652248726340294E-4	9.700057356860892E-4
22-23	0.0	0.0	0.0	3.0927719108831835E-4	0.0010121798981072236
24-25	0.0	0.0	0.0	0.001068412114668736	0.0010121798981072236
26-27	0.0	0.0	0.0	0.002403927258004656	0.0010402960063879798
28-29	0.0	0.0	0.0	0.00830830999696346	0.0010402960063879798
30-31	0.0	0.0	0.0	0.03410483934455728	0.0010402960063879798
32-33	0.0	0.0	0.0	0.0931064925717242	0.001068412114668736
34-35	0.0	0.0	0.0	0.1761895925413588	0.0011527604395110047
36-37	0.0	0.0	0.0	0.3114843055883577	0.001180876547791761
38-39	0.0	0.0	0.0	0.5253494832259298	0.0012933409809147856
40-41	0.0	2.811610828075621E-5	0.0	0.7662061248130279	0.0012933409809147856
42-43	0.0	2.811610828075621E-5	0.0	1.0240870699641238	0.0012933409809147856
44-45	0.0	2.811610828075621E-5	0.0	1.3366819618295713	0.0014198634681781886
46-47	0.0	2.811610828075621E-5	0.0	1.707912997514536	0.0015604440095819698
48-49	0.0	2.811610828075621E-5	0.0	2.1025928675056513	0.0016729084427049945
50-51	0.0	2.811610828075621E-5	0.0	2.5210027328857247	0.001743198713406885
52-53	0.0	2.811610828075621E-5	0.0	2.9483675787532193	0.0019118953630914224
54-55	0.0	2.811610828075621E-5	0.0	3.429616946140783	0.0019400114713721787
56-57	0.0	2.811610828075621E-5	0.0	3.984938200793999	0.0019400114713721787
58-59	0.0	2.811610828075621E-5	0.0	4.572030657804469	0.0019400114713721787
60-61	0.0	2.811610828075621E-5	0.0	5.194127669624481	0.001982185633793313
62-63	1.4058054140378106E-5	2.811610828075621E-5	0.0	5.815676417333019	0.002024359796214447
64-65	2.811610828075621E-5	2.811610828075621E-5	0.0	6.487342128052004	0.0020524759044952033
66-67	2.811610828075621E-5	2.811610828075621E-5	0.0	7.2600430738778865	0.0020665339586355812
68-69	2.811610828075621E-5	2.811610828075621E-5	0.0	8.095934973064768	0.002094650066916338
70-71	2.811610828075621E-5	2.811610828075621E-5	0.0	8.962051688653464	0.0021649403376182283
72-73	2.811610828075621E-5	2.811610828075621E-5	0.0	9.836659468942948	0.002277404770741253
74-75	2.811610828075621E-5	2.811610828075621E-5	0.0	10.735123767108652	0.002403927258004656
76-77	2.811610828075621E-5	2.811610828075621E-5	0.0	11.723123812094425	0.0024461014204257905
78-79	2.811610828075621E-5	2.811610828075621E-5	0.0	12.791957668387372	0.0024882755828469247
80-81	2.811610828075621E-5	2.811610828075621E-5	0.0	13.877351912457684	0.002530449745268059
82-83	2.811610828075621E-5	2.811610828075621E-5	0.0	14.980220317824488	0.0025866819618295712
84-85	2.811610828075621E-5	2.811610828075621E-5	0.0	16.086195553156315	0.0026429141783910837
86	2.811610828075621E-5	2.811610828075621E-5	0.0	16.944734977563346	0.0026429141783910837
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3310	0.0	47.553677	1
GTATCAA	6080	0.0	41.860798	1
TCAACGC	7860	0.0	31.908806	4
ATCAACG	7910	0.0	31.70711	3
TATCAAC	8155	0.0	30.980736	2
CAACGCA	8150	0.0	30.604626	5
AACGCAG	8645	0.0	28.793135	6
GTACATG	11365	0.0	26.201178	1
TACATGG	11565	0.0	25.215965	2
ACGCAGA	10060	0.0	24.28446	7
ACATGGG	12375	0.0	23.082808	3
CGCAGAG	10585	0.0	23.035711	8
TATAGCG	145	3.0694086E-5	22.134811	5
CATGGGG	8400	0.0	21.560736	4
TAAGGCG	1145	0.0	21.223461	5
GCAGAGT	12245	0.0	19.01454	9
GAGTACA	8810	0.0	18.806698	1
AGTACAT	8760	0.0	17.901363	2
AGAGTAC	10125	0.0	17.654797	10-11
GTATTAG	845	0.0	17.429272	1
>>END_MODULE