FastQCFastQC Report
Mon 15 Aug 2016
SRR2920856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3556680
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38250.047.9822041
GTATCAA67650.041.6081351
TCAACGC87900.031.4816364
ATCAACG88150.031.44623
CAACGCA88950.030.8909115
TATCAAC92800.029.9186972
AACGCAG94000.029.2317586
GTACATG114650.026.0441341
TACATGG118600.024.7708422
ACGCAGA110850.024.4886347
CGCAGAG112250.023.8426328
ACATGGG122300.023.3640233
TAAGGCG11000.021.5712575
CATGGGG82000.020.5490684
GCAGAGT125900.020.2022869
AGAGTAC104500.019.16152610-11
GAGTACT49700.017.61365312-13
CAGAGTA117200.017.6114710-11
GAGTACA90950.016.7813421
GTATAGT8500.016.2219621