##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920856_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3556680 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.388993949413496 31.0 30.0 34.0 27.0 34.0 2 30.41928455750869 31.0 30.0 34.0 27.0 34.0 3 30.550727644882308 31.0 30.0 34.0 27.0 34.0 4 34.20383644297491 35.0 35.0 37.0 30.0 37.0 5 33.7578351158946 35.0 35.0 37.0 28.0 37.0 6 33.70901767940889 36.0 35.0 37.0 29.0 37.0 7 33.528463342218025 36.0 35.0 37.0 28.0 37.0 8 33.46755204291643 36.0 35.0 37.0 28.0 37.0 9 34.81862298548084 38.0 35.0 39.0 28.0 39.0 10-11 34.639656646085676 38.0 34.5 39.0 27.0 39.0 12-13 34.467492577347414 38.0 34.0 39.0 27.0 39.0 14-15 35.38630084798183 39.0 34.0 40.0 27.0 41.0 16-17 35.269362720289706 38.0 34.0 40.0 27.0 41.0 18-19 35.194016048674605 38.5 34.0 40.0 26.0 41.0 20-21 35.08867609680938 39.0 34.0 40.0 25.5 41.0 22-23 34.94228184711585 38.0 34.0 40.0 25.0 41.0 24-25 34.7674198972075 38.0 34.0 40.0 25.0 41.0 26-27 34.59186657219654 38.0 33.5 40.0 24.0 41.0 28-29 34.540459501557635 38.0 33.5 40.0 24.0 41.0 30-31 34.51926867190751 38.0 33.5 40.0 24.0 41.0 32-33 34.37072058773913 38.0 33.0 40.0 24.0 41.0 34-35 34.20215692724676 38.0 33.0 40.0 23.0 41.0 36-37 34.00378695862433 38.0 33.0 40.0 22.0 41.0 38-39 33.82753213671177 38.0 33.0 40.0 21.0 41.0 40-41 33.60005946556902 38.0 32.5 40.0 19.0 41.0 42-43 33.607355173926244 38.0 33.0 40.0 19.0 41.0 44-45 33.45911678869058 38.0 32.5 40.0 18.0 41.0 46-47 33.210810221892324 38.0 32.0 40.0 15.5 41.0 48-49 32.95351423237401 37.5 32.0 40.0 14.5 41.0 50-51 32.703786115141085 37.0 31.0 40.0 12.5 41.0 52-53 32.45237159373348 37.0 31.0 40.0 9.0 41.0 54-55 32.17885851411991 37.0 31.0 40.0 8.0 41.0 56-57 31.85977484620489 36.0 30.5 39.5 7.5 41.0 58-59 31.537376851445728 36.0 30.0 39.0 6.5 41.0 60-61 31.216913104355747 35.5 29.5 39.0 2.0 40.0 62-63 30.90943689058335 35.0 29.0 39.0 2.0 40.0 64-65 30.518568159069694 35.0 29.0 38.5 2.0 40.0 66-67 30.095041583724147 34.5 28.5 38.0 2.0 40.0 68-69 29.65251048730839 34.0 28.0 37.0 2.0 40.0 70-71 29.23112790579979 34.0 28.0 37.0 2.0 39.0 72-73 28.798994849128963 34.0 27.0 36.0 2.0 39.0 74-75 28.35823197476298 34.0 26.0 36.0 2.0 38.5 76-77 26.87015292351294 31.5 25.0 34.5 2.0 36.5 78-79 27.704041549984815 33.0 26.0 35.0 2.0 37.0 80-81 27.73314256553865 33.0 26.0 35.0 2.0 37.0 82-83 27.394466890470888 33.0 26.0 35.0 2.0 36.0 84-85 27.082324949109847 33.0 25.5 35.0 2.0 36.0 86-87 26.540957297254742 33.0 25.0 35.0 2.0 35.0 88-89 26.17526850883408 32.5 24.0 35.0 2.0 35.0 90-91 25.812992313055997 32.0 23.0 35.0 2.0 35.0 92-93 25.51399127838321 32.0 21.0 35.0 2.0 35.0 94-95 25.14705680578517 32.0 19.5 34.5 2.0 35.0 96-97 24.628574682006814 32.0 15.5 34.0 2.0 35.0 98-99 24.04271778737474 32.0 2.0 34.0 2.0 35.0 100-101 21.870876350979003 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72183.0 3 33916.0 4 18668.0 5 13308.0 6 11605.0 7 11522.0 8 13279.0 9 15311.0 10 17355.0 11 19725.0 12 22387.0 13 24262.0 14 26421.0 15 28433.0 16 30294.0 17 31167.0 18 30191.0 19 28120.0 20 28620.0 21 29921.0 22 32000.0 23 36504.0 24 42786.0 25 50058.0 26 59542.0 27 70804.0 28 84444.0 29 98684.0 30 116278.0 31 138982.0 32 165831.0 33 203399.0 34 256375.0 35 333709.0 36 449782.0 37 547093.0 38 336287.0 39 27433.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.20200002535601 19.52257459243743 13.785045873239696 24.49037950896687 2 16.13350090533869 20.89088700698404 37.93872375361292 25.03688833406435 3 18.835068302092647 24.605596891115947 29.807775584462327 26.751559222329075 4 13.648976818841877 16.648810347329313 36.305974640089715 33.396238193739094 5 14.210804386268041 37.22501822737928 34.37659832254687 14.187579063805808 6 33.24809983717916 35.72536895033331 17.007079962341525 14.019451250146004 7 28.81515893797337 31.950699664949177 21.157445730213702 18.076695666863753 8 26.662278963345976 34.38316360540847 20.217112493356517 18.737444937889038 9 26.73641906171288 15.418928255886044 19.71708806978956 38.127564612611515 10-11 25.48682388152211 25.486472036099904 27.94872852212397 21.07797556025402 12-13 26.040728277227114 22.942007726148308 27.754011304794147 23.263252691830434 14-15 23.28428923398936 24.56450151478346 25.18920367398674 26.962005577240443 16-17 23.29769528959152 27.456223556501673 26.079083230631827 23.166997923274977 18-19 23.329614918230742 26.613047205300834 27.551401848121245 22.505936028347183 20-21 24.104531775672413 25.932629833944915 27.096319095104608 22.866519295278064 22-23 23.870090081014666 25.737400602903765 26.950003672124577 23.44250564395699 24-25 23.56530144451659 25.932375423525695 27.05348773618133 23.448835395776392 26-27 23.39261805482681 26.12065116506758 26.99266183119708 23.49406894890853 28-29 23.443594500259678 26.243431871261514 26.95018734539944 23.36278628307937 30-31 23.672595054486592 26.02557659549995 26.717074977452516 23.58475337256094 32-33 23.46880186839057 26.196997474528374 26.52323176556468 23.810968891516378 34-35 23.84556491612398 26.096441429502455 26.628782259805106 23.429211394568465 36-37 23.960386294096473 25.841685790136786 26.63685655147588 23.561071364290857 38-39 23.686781740930186 25.91600926886602 26.692441329197464 23.70476766100633 40-41 23.854561960375094 25.805602923812394 26.75868803044968 23.58114708536284 42-43 23.721521268951083 25.82987847940592 26.538002268414417 23.91059798322858 44-45 23.594814226412222 25.80307227710256 26.727514306690274 23.874599189794942 46-47 23.647746453857575 25.818456576727133 26.549756385742334 23.984040583672957 48-49 23.418579671349356 25.919366993217785 26.552401894071465 24.109651441361393 50-51 23.38239954647544 25.979859662719228 26.6093190185895 24.02842177221583 52-53 23.585964326244245 25.819847983327204 26.59427148180265 23.9999162086259 54-55 23.233897073544927 25.78376142496249 26.686974586699243 24.29536691479334 56-57 23.149024088460116 25.86859733127738 26.561751589450903 24.4206269908116 58-59 23.124899481408416 25.65228833736907 26.657938185143156 24.564873996079353 60-61 23.060359040258906 25.633198266597468 26.788574976802614 24.517867716341012 62-63 22.98276673155586 25.6165012193788 26.84136321854142 24.55936883052392 64-65 22.78290574546391 25.603380137851993 26.628426933466574 24.98528718321752 66-67 22.7886309779131 25.664118809374802 26.578070613384337 24.96917959932776 68-69 22.994631338597124 25.466148842324344 26.534984660565414 25.004235158513115 70-71 22.805522145077372 25.401842046132987 26.4614502450409 25.331185563748736 72-73 22.766089381318448 25.456802892394663 26.355437297202496 25.421670429084394 74-75 22.653514415272923 25.707671707356262 26.20616434048496 25.432649536885855 76-77 22.704037693521386 25.47971765942266 26.379728287083275 25.43651635997268 78-79 22.64057608517187 25.668110031108522 26.219596524838767 25.47171735888084 80-81 22.636996851461625 25.642372552247977 26.12728654989343 25.593344046396965 82-83 22.516792045208824 25.61346816646641 26.203013977741453 25.666725810583312 84-85 22.402243394842973 25.620480187165157 26.100526753495814 25.876749664496057 86-87 22.388255162225253 25.646828797943005 26.0725643657032 25.892351674128538 88-89 22.327654280678953 25.59688902180282 25.932117887615362 26.143338809902865 90-91 22.141244433393688 25.59274472840938 25.958674123670917 26.30733671452602 92-93 22.24310135209295 25.47799805259792 25.94536608873799 26.333534506571144 94-95 21.996526774664773 25.399756202243328 26.03861822820522 26.565098794886687 96-97 22.103260757248552 25.24643116776636 26.052331204051825 26.59797687093326 98-99 21.936521843285735 25.38001586925801 25.848263013550728 26.83519927390553 100-101 21.966860401116207 25.21280399088936 25.955219182451966 26.865116425542467 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 106.0 1 87.0 2 128.0 3 189.5 4 170.0 5 180.0 6 272.0 7 531.5 8 813.0 9 863.0 10 744.5 11 650.0 12 654.0 13 682.5 14 732.5 15 832.5 16 932.0 17 1019.5 18 1123.5 19 1266.0 20 1453.0 21 1718.5 22 2076.5 23 2518.5 24 3028.5 25 3825.5 26 5122.0 27 6942.5 28 9512.0 29 13165.0 30 18051.0 31 24790.5 32 33481.0 33 40727.0 34 47221.0 35 55173.5 36 64491.0 37 73943.5 38 82257.0 39 89498.0 40 97367.0 41 105320.0 42 112542.0 43 121262.0 44 129670.0 45 137575.0 46 144271.5 47 148925.0 48 154022.5 49 157253.0 50 157368.5 51 156063.5 52 153338.5 53 150769.5 54 146408.5 55 137625.0 56 125878.5 57 112749.0 58 100792.0 59 88111.0 60 73332.0 61 59613.0 62 48552.0 63 37704.0 64 27365.0 65 20457.5 66 15441.5 67 11621.0 68 8771.5 69 6480.5 70 4898.5 71 3751.5 72 2847.0 73 2362.0 74 1822.0 75 1316.5 76 911.0 77 540.5 78 303.5 79 167.0 80 81.5 81 45.0 82 26.0 83 15.5 84 8.5 85 3.5 86 2.0 87 3.0 88 2.0 89 1.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2031388823284636 2 0.0 3 0.011808765477917607 4 0.014507911872870205 5 0.006101195496924098 6 0.10388902009739419 7 0.14001821923816593 8 0.12168651663911288 9 0.13984952258848138 10-11 0.11200051733639235 12-13 0.1107634085720391 14-15 0.11322356804660526 16-17 0.06425936547566832 18-19 0.07722089139309693 20-21 0.035524702812735474 22-23 0.08094627574029713 24-25 0.056119752128389405 26-27 0.06328935973998223 28-29 0.03791457201659975 30-31 0.023926808146923537 32-33 0.03111047381265675 34-35 0.019329824443019897 36-37 0.023617530955835214 38-39 0.031518157382727716 40-41 0.025627832697909286 42-43 0.01269442288876143 44-45 0.025684064914470797 46-47 0.03265685976809834 48-49 0.008997154649841988 50-51 0.01498588571364306 52-53 0.006410472688012416 54-55 0.012146158777286684 56-57 0.00518742197779952 58-59 0.0034863974268137705 60-61 0.022028970837972492 62-63 0.010149915089352991 64-65 0.007225839828154346 66-67 0.03950313213446247 68-69 0.08699123902065972 70-71 0.020890268452601866 72-73 0.04395953529696234 74-75 0.0834064352148633 76-77 0.2624076385842977 78-79 0.07958264448868045 80-81 0.01646198139838276 82-83 0.010768469471529629 84-85 0.013003700079849747 86-87 0.2744132168201806 88-89 0.6249929709729298 90-91 0.26460069503019673 92-93 0.3506359863693107 94-95 0.001223050710212895 96-97 0.004484519270780616 98-99 0.0033177007771292327 100-101 0.00504684143639574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3556680.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.25554789284989 #Duplication Level Percentage of deduplicated Percentage of total 1 74.01521814941417 40.89751431254687 2 13.354899542860005 14.758645825894003 3 5.175045356949702 8.578498997058142 4 2.5587584786171877 5.65542406645871 5 1.3800725729701373 3.8128333075680003 6 0.8675954483916642 2.876367711013451 7 0.5536971139564147 2.1416386178846007 8 0.4008289250863467 1.7718417493558538 9 0.2884408481705576 1.4344161390308243 >10 1.3169428351927848 12.532317316858443 >50 0.06334245022025024 2.3959949755750687 >100 0.023372094886418122 2.19874038006544 >500 0.0011737775946650067 0.43914318825770415 >1k 6.124056897174499E-4 0.5066234124328671 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.623221656151242E-5 2 0.0 0.0 0.0 0.0 5.623221656151242E-5 3 0.0 0.0 0.0 0.0 5.623221656151242E-5 4 0.0 0.0 0.0 0.0 5.623221656151242E-5 5 0.0 0.0 0.0 2.811610828075621E-5 1.1246443312302484E-4 6 0.0 0.0 0.0 2.811610828075621E-5 1.6869664968453726E-4 7 0.0 0.0 0.0 2.811610828075621E-5 1.6869664968453726E-4 8 0.0 0.0 0.0 2.811610828075621E-5 1.968127579652935E-4 9 0.0 0.0 0.0 5.623221656151242E-5 1.968127579652935E-4 10-11 0.0 0.0 0.0 5.623221656151242E-5 2.530449745268059E-4 12-13 0.0 0.0 0.0 5.623221656151242E-5 5.482641114747461E-4 14-15 0.0 0.0 0.0 5.623221656151242E-5 6.74786598738149E-4 16-17 0.0 0.0 0.0 5.623221656151242E-5 7.731929777207958E-4 18-19 0.0 0.0 0.0 5.623221656151242E-5 8.153671401419301E-4 20-21 0.0 0.0 0.0 9.840637898264674E-5 8.997154649841988E-4 22-23 0.0 0.0 0.0 3.233352452286964E-4 9.418896274053331E-4 24-25 0.0 0.0 0.0 0.0010965282229494922 9.559476815457112E-4 26-27 0.0 0.0 0.0 0.002432043366285412 0.0010121798981072236 28-29 0.0 0.0 0.0 0.008322368051103838 0.0010121798981072236 30-31 0.0 0.0 0.0 0.03411889739869767 0.0010121798981072236 32-33 0.0 0.0 0.0 0.09261446067681095 0.0010824701688091141 34-35 0.0 0.0 0.0 0.17505089015598818 0.0012230507102128953 36-37 0.0 0.0 0.0 0.3098535713080738 0.0012652248726340294 38-39 0.0 0.0 0.0 0.5233672975921364 0.0012652248726340294 40-41 0.0 0.0 0.0 0.7651236546442188 0.0012652248726340294 42-43 0.0 0.0 0.0 1.0242979407762296 0.0012933409809147856 44-45 1.4058054140378106E-5 0.0 0.0 1.3381439994601707 0.00133551514333592 46-47 2.811610828075621E-5 0.0 0.0 1.7093750351451353 0.0013917473598974324 48-49 2.811610828075621E-5 0.0 0.0 2.1030848994005646 0.0015042117930204573 50-51 2.811610828075621E-5 0.0 0.0 2.516026181720031 0.001602618172003104 52-53 2.811610828075621E-5 0.0 0.0 2.939145495237131 0.001602618172003104 54-55 2.811610828075621E-5 0.0 0.0 3.41539019535072 0.001602618172003104 56-57 2.811610828075621E-5 0.0 0.0 3.965214750835048 0.0016307342802838601 58-59 2.811610828075621E-5 0.0 0.0 4.546965147272175 0.0016588503885646164 60-61 4.2174162421134315E-5 0.0 0.0 5.1641840143054765 0.0016588503885646164 62-63 5.623221656151242E-5 0.0 0.0 5.782921151185937 0.001743198713406885 64-65 5.623221656151242E-5 0.0 0.0 6.4485981308411215 0.0018275470382491537 66-67 5.623221656151242E-5 0.0 0.0 7.212470618666846 0.00185566314652991 68-69 5.623221656151242E-5 0.0 0.0 8.038606228280306 0.0018837792548106662 70-71 5.623221656151242E-5 0.0 0.0 8.895796079489863 0.0018837792548106662 72-73 5.623221656151242E-5 0.0 0.0 9.762685988056276 0.0020103017420740692 74-75 5.623221656151242E-5 0.0 0.0 10.6595898422124 0.0021649403376182283 76-77 5.623221656151242E-5 0.0 0.0 11.64556552740196 0.002207114500039363 78-79 5.623221656151242E-5 0.0 0.0 12.709408774475072 0.0022633467166008753 80-81 5.623221656151242E-5 0.0 0.0 13.787689643150355 0.0023336369873027656 82-83 5.623221656151242E-5 0.0 0.0 14.886326574220902 0.0024320433662854126 84-85 5.623221656151242E-5 0.0 0.0 15.989194979587705 0.0024461014204257905 86-87 5.623221656151242E-5 0.0 0.0 17.139607724057267 0.002544507799408437 88-89 5.623221656151242E-5 0.0 0.0 18.3484316834801 0.0026429141783910837 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3825 0.0 47.982204 1 GTATCAA 6765 0.0 41.608135 1 TCAACGC 8790 0.0 31.481636 4 ATCAACG 8815 0.0 31.4462 3 CAACGCA 8895 0.0 30.890911 5 TATCAAC 9280 0.0 29.918697 2 AACGCAG 9400 0.0 29.231758 6 GTACATG 11465 0.0 26.044134 1 TACATGG 11860 0.0 24.770842 2 ACGCAGA 11085 0.0 24.488634 7 CGCAGAG 11225 0.0 23.842632 8 ACATGGG 12230 0.0 23.364023 3 TAAGGCG 1100 0.0 21.571257 5 CATGGGG 8200 0.0 20.549068 4 GCAGAGT 12590 0.0 20.202286 9 AGAGTAC 10450 0.0 19.161526 10-11 GAGTACT 4970 0.0 17.613653 12-13 CAGAGTA 11720 0.0 17.61147 10-11 GAGTACA 9095 0.0 16.781342 1 GTATAGT 850 0.0 16.221962 1 >>END_MODULE