FastQCFastQC Report
Mon 15 Aug 2016
SRR2920855_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920855_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572733
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.042.95571
GTATCAA41300.042.569381
TCAACGC53500.032.5653574
ATCAACG54150.032.259123
CAACGCA55850.031.1130125
TATCAAC56150.031.0290372
AACGCAG58650.029.7799476
GTACATG86350.026.1699681
TACATGG88100.024.6161232
ACGCAGA70300.024.5840237
ACATGGG91800.023.1240733
CGCAGAG73950.023.12228
CATGGGG63750.020.568944
GCAGAGT83400.019.4032979
TAAGGCG4800.019.103585
TATACCG2402.0537482E-719.103585
GTGGTAT15950.018.1787451
GAGTACA66000.017.7819861
AGAGTAC69050.017.65976510-11
AGTACAT64800.017.3350682
Produced by FastQC (version 0.11.5)