##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920855_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2572733
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.761450955073844	31.0	30.0	33.0	23.0	34.0
2	28.78890580561605	31.0	29.0	34.0	22.0	34.0
3	28.862507691237294	31.0	29.0	34.0	22.0	34.0
4	32.346686189355836	35.0	33.0	37.0	24.0	37.0
5	32.317388162704795	35.0	33.0	37.0	24.0	37.0
6	32.44795243035325	35.0	33.0	37.0	25.0	37.0
7	32.28771971285011	35.0	32.0	37.0	23.0	37.0
8	32.23393760642865	35.0	32.0	37.0	23.0	37.0
9	33.51240684517204	37.0	33.0	39.0	23.0	39.0
10-11	33.3284231204715	37.0	33.0	39.0	22.0	39.0
12-13	33.16835598563861	37.0	33.0	39.0	19.0	39.0
14-15	33.951620902752055	38.0	33.0	40.0	18.5	41.0
16-17	33.843314094389115	38.0	33.0	40.0	18.0	41.0
18-19	33.72560502780506	38.0	32.0	40.0	17.5	41.0
20-21	33.63504219054212	38.0	32.0	40.0	18.0	41.0
22-23	33.41883106408632	38.0	32.0	40.0	17.5	41.0
24-25	33.23216925347481	38.0	32.0	40.0	16.0	41.0
26-27	33.26729590672643	38.0	32.0	40.0	16.0	41.0
28-29	33.11459739506587	38.0	32.0	40.0	14.0	41.0
30-31	32.87041348635867	38.0	31.5	40.0	10.0	41.0
32-33	32.63310456234673	38.0	31.0	40.0	9.0	41.0
34-35	32.31988881084823	37.5	30.5	40.0	8.5	41.0
36-37	32.021016755333726	37.0	30.0	40.0	8.0	41.0
38-39	31.828325753197085	37.0	30.0	40.0	4.5	41.0
40-41	31.629787467257582	37.0	30.0	40.0	2.0	41.0
42-43	31.40050483279843	37.0	30.0	40.0	2.0	41.0
44-45	31.126237157139897	36.0	30.0	40.0	2.0	41.0
46-47	30.878052250272376	36.0	29.0	39.0	2.0	41.0
48-49	30.579451890266107	36.0	28.5	39.0	2.0	40.0
50-51	29.41552485236517	34.5	27.0	38.0	2.0	39.5
52-53	29.85807505092833	34.5	28.0	38.5	2.0	39.5
54-55	30.515163641155148	36.0	29.0	39.0	2.0	40.0
56-57	30.46746086748994	36.0	28.5	39.0	2.0	41.0
58-59	30.224043847534897	35.0	28.0	39.0	2.0	41.0
60-61	29.898773016865725	35.0	28.0	39.0	2.0	40.0
62-63	29.50218619654663	35.0	28.0	39.0	2.0	40.0
64-65	29.077267054140478	34.0	26.5	38.0	2.0	40.0
66-67	28.694323312990505	34.0	26.0	37.5	2.0	40.0
68-69	28.249449321013877	34.0	26.0	37.0	2.0	39.5
70-71	27.92402903838059	34.0	26.0	36.5	2.0	39.0
72-73	27.46758058453792	33.5	25.0	36.0	2.0	39.0
74-75	27.029436012209583	33.0	24.5	35.5	2.0	38.5
76-77	26.616087833444045	33.0	23.5	35.0	2.0	37.5
78-79	26.159839750180062	33.0	22.0	35.0	2.0	37.0
80-81	25.7075011281777	32.0	20.0	35.0	2.0	36.0
82-83	25.23515304541902	32.0	18.5	35.0	2.0	36.0
84-85	24.83097488157535	32.0	17.0	35.0	2.0	35.5
86-87	24.36823992229275	31.5	9.0	34.0	2.0	35.0
88-89	23.822725871670322	31.0	3.5	34.0	2.0	35.0
90-91	23.315912688957617	31.0	2.0	34.0	2.0	35.0
92-93	22.779298901207394	31.0	2.0	34.0	2.0	35.0
94-95	22.14111588726852	30.0	2.0	34.0	2.0	35.0
96-97	21.49046675267119	30.0	2.0	34.0	2.0	35.0
98	21.088691675350688	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	124494.0
3	21092.0
4	15654.0
5	13196.0
6	11924.0
7	12203.0
8	13947.0
9	16154.0
10	17690.0
11	18537.0
12	19375.0
13	19741.0
14	18254.0
15	19267.0
16	20612.0
17	22030.0
18	22522.0
19	22589.0
20	23709.0
21	25204.0
22	27334.0
23	30591.0
24	34920.0
25	40633.0
26	47180.0
27	55221.0
28	64017.0
29	73968.0
30	85879.0
31	100903.0
32	121826.0
33	146674.0
34	182493.0
35	233362.0
36	298975.0
37	330387.0
38	198820.0
39	21356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.05207688990445	17.980381405459408	13.204481016560873	24.763060688075274
2	16.266272999146207	19.622037190538915	38.68483218846276	25.426857621852122
3	19.385017070764167	23.430100060880932	28.969837114352735	28.215045754002162
4	13.757226512158969	15.615486380082563	35.65666355272074	34.97062355503773
5	14.944869014096007	36.308578008955166	33.693214268080325	15.053338708868505
6	33.932693216097306	34.257404263309866	17.112178880330063	14.697723640262769
7	29.56583939789762	31.176136717049204	20.701467935560117	18.556555949493063
8	26.955621562849014	33.894200959943525	19.941510851746763	19.208666625460697
9	27.018855046365093	15.588831021330234	19.345925130979392	38.04638880132528
10-11	26.047422341809728	25.0104315159077	27.2816936856047	21.66045245667787
12-13	26.499522079388075	22.383049386166462	27.190553692116502	23.926874842328957
14-15	23.768992896206406	24.00997108222897	24.62025868762413	27.600777333940492
16-17	23.964825555348888	26.923038798670344	25.341708682379092	23.770426963601672
18-19	23.955280712624937	26.181951731741826	26.757575586720467	23.105191968912774
20-21	24.775960025441186	25.442118387091128	26.28895195156061	23.492969635907084
22-23	24.534167169884146	25.17487455877684	26.200193142131447	24.090765129207572
24-25	24.19445866284144	25.40180800963359	26.23383705354017	24.169896273984794
26-27	24.056229000432754	25.60558983560165	26.20203346315989	24.1361477008057
28-29	24.16993780780586	25.695729230797955	26.175306459221936	23.959026502174257
30-31	24.31202588418813	25.549999134678785	25.94238827487813	24.19558670625495
32-33	24.073997398696005	25.599887099285564	25.87205013351559	24.45406536850284
34-35	24.396695672085652	25.525217316338512	25.97612665407054	24.101960357505295
36-37	24.505989565674167	25.313255486600056	25.938349655247485	24.242405292478292
38-39	24.29580422175648	25.409958063703325	25.938742145489847	24.355495569050348
40-41	24.43338291098565	25.35621484586976	26.097027645889764	24.11337459725483
42-43	24.41139427217264	25.229672058269685	25.912435764549507	24.44649790500816
44-45	24.246198936104182	25.257590904850147	25.996236118197285	24.499974040848386
46-47	24.338054911301825	25.213355169972708	25.915648661198137	24.532941257527327
48-49	24.10279258698073	25.360524239929777	25.89025980539673	24.646423367692766
50-51	23.962357453923534	25.487597544878255	26.01237084109698	24.53767416010123
52-53	24.1276134882611	25.24321214613702	26.106742685474526	24.522431680127355
54-55	23.94235660673782	25.175680729261764	26.236297293681936	24.645665370318476
56-57	23.81319206264023	25.310109851538098	26.14375244389462	24.732945641927053
58-59	23.87512184610364	25.043595237881462	26.19112495818097	24.890157957833928
60-61	23.718707820443594	24.95011172396937	26.461830929989304	24.86934952559773
62-63	23.73173891209012	25.047999124041603	26.356432702368735	24.86382926149954
64-65	23.660046761594565	25.013918536092216	26.261167782265083	25.06486692004814
66-67	23.686626889588627	25.028151069037456	26.206931305922478	25.078290735451443
68-69	23.76779434020567	24.88739303680508	26.21726297198056	25.127549651008696
70-71	23.579527772601914	24.765842289745837	26.286616256461837	25.36801368119041
72-73	23.616685781214734	24.85331478256527	26.231103372087194	25.298896064132805
74-75	23.654250010386168	25.000980103221938	26.20483942788498	25.139930458506914
76-77	23.72170286316792	24.861477705073362	26.294397690102087	25.122421741656627
78-79	23.66154154012806	24.968174779992992	26.191039476925422	25.179244202953527
80-81	23.680680753710558	24.882049845522655	26.245035910764802	25.192233490001986
82-83	23.67656975416179	24.831432554588503	26.29519506462687	25.196802626622837
84-85	23.734922344355642	24.822633887982605	26.225353030297416	25.21709073736434
86-87	23.754556429514547	24.7986589616119	26.326537776676933	25.120246832196624
88-89	23.732858629720646	24.709778683684814	26.206378995652972	25.35098369094157
90-91	23.765198487447154	24.692518856190432	26.217459123462206	25.324823532900208
92-93	23.940920752579466	24.53667934463629	26.23629709400182	25.286102808782424
94-95	23.87954567355616	24.5169572628365	26.32375655387314	25.2797405097342
96-97	24.06710896975606	24.35733501562223	26.254747774683306	25.3208082399384
98	24.15998489457215	24.271637101458772	26.145069064809384	25.42330893915969
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	66.0
1	50.5
2	79.5
3	127.5
4	128.5
5	155.5
6	228.0
7	436.5
8	683.5
9	752.5
10	744.5
11	804.5
12	923.0
13	1057.0
14	1162.5
15	1324.0
16	1464.5
17	1506.0
18	1522.5
19	1481.0
20	1466.5
21	1488.0
22	1565.0
23	1725.0
24	1917.5
25	2254.0
26	2861.5
27	3754.0
28	5206.0
29	7566.5
30	11234.5
31	16054.0
32	21055.0
33	26413.0
34	32474.5
35	38867.5
36	45367.5
37	52347.5
38	59132.0
39	64963.5
40	70471.0
41	75857.5
42	83001.0
43	90829.0
44	96851.5
45	102173.0
46	107557.5
47	111836.5
48	114845.5
49	116100.0
50	115896.0
51	115759.0
52	114938.0
53	113670.0
54	111169.0
55	106978.5
56	99631.5
57	91183.0
58	83901.5
59	74008.5
60	62379.5
61	51192.5
62	40816.5
63	31076.0
64	23152.0
65	17824.5
66	13547.5
67	10141.0
68	7583.5
69	5708.0
70	4234.5
71	3174.5
72	2518.0
73	1980.5
74	1505.5
75	1057.5
76	680.0
77	426.5
78	250.0
79	121.0
80	69.0
81	39.0
82	28.0
83	20.5
84	12.5
85	7.0
86	8.0
87	9.0
88	5.0
89	4.0
90	6.0
91	4.5
92	2.5
93	3.5
94	3.5
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4029955693031496
2	0.026469905738372383
3	0.019395716539570954
4	0.016869220397141872
5	0.022777334453283726
6	0.023321502853191525
7	0.016052967797280167
8	0.011272059712375906
9	0.0
10-11	1.943458571099294E-5
12-13	0.005052992284858165
14-15	0.00363426752795568
16-17	0.007132492955934409
18-19	0.03892747517911886
20-21	0.08154752164332638
22-23	0.10125419155427322
24-25	0.06720479738861358
26-27	0.07756343157257282
28-29	0.05869244884719868
30-31	0.055718957233416765
32-33	0.02182503975344507
34-35	0.3594037935533924
36-37	0.5503291635781871
38-39	0.5013345730007739
40-41	0.5096525756850789
42-43	0.45649898376551323
44-45	0.42818279238459644
46-47	0.42691954431338197
48-49	0.41590013421524896
50-51	0.4111969644731886
52-53	0.4786349768903342
54-55	0.4794706640759068
56-57	0.44713151345281454
58-59	0.4314478027840433
60-61	0.44738416306705747
62-63	0.4262976375706301
64-65	0.4780325047332934
66-67	0.5007709700151551
68-69	0.43842481905428976
70-71	0.3922093742335485
72-73	0.37611753726484637
74-75	0.3586652792963747
76-77	0.3559055681254137
78-79	0.3545451471256442
80-81	0.37213344719409286
82-83	0.36698328198067964
84-85	0.3677606654091194
86-87	0.35168826302612827
88-89	0.4000415122750787
90-91	0.37504863505074176
92-93	0.3639126174383428
94-95	0.3713560637656531
96-97	0.37153097503705207
98	0.36599211810941906
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2572733.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.182979681760784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55383204225097	45.30683886317466
2	12.170947581475916	14.406258868445294
3	4.600051060287972	8.167341853082553
4	2.317850044816671	5.487090884310134
5	1.241075542702665	3.6725274313651033
6	0.7808147813663765	2.772656720449485
7	0.5227853952097217	2.1657998195832766
8	0.34884335054375376	1.6516471141878666
9	0.25885021786698426	1.3787574466176973
>10	1.14570938932783	11.42122762777943
>50	0.04413779137101802	1.745903456472855
>100	0.014508454669933371	1.5119059897376208
>500	3.9623207339650615E-4	0.16794247806130078
>1k	1.9811603669776914E-4	0.1441014467327899
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.886917142198588E-5
2	0.0	0.0	0.0	0.0	3.886917142198588E-5
3	0.0	0.0	0.0	0.0	3.886917142198588E-5
4	0.0	0.0	0.0	0.0	3.886917142198588E-5
5	0.0	0.0	0.0	0.0	3.886917142198588E-5
6	0.0	0.0	0.0	0.0	3.886917142198588E-5
7	0.0	0.0	0.0	0.0	3.886917142198588E-5
8	3.886917142198588E-5	0.0	0.0	0.0	3.886917142198588E-5
9	3.886917142198588E-5	0.0	0.0	0.0	3.886917142198588E-5
10-11	3.886917142198588E-5	0.0	0.0	3.886917142198588E-5	7.773834284397177E-5
12-13	3.886917142198588E-5	0.0	0.0	3.886917142198588E-5	7.773834284397177E-5
14-15	3.886917142198588E-5	0.0	0.0	5.8303757132978824E-5	7.773834284397177E-5
16-17	3.886917142198588E-5	0.0	0.0	7.773834284397177E-5	7.773834284397177E-5
18-19	3.886917142198588E-5	0.0	0.0	7.773834284397177E-5	7.773834284397177E-5
20-21	3.886917142198588E-5	0.0	0.0	1.1660751426595765E-4	7.773834284397177E-5
22-23	3.886917142198588E-5	0.0	0.0	3.1095337137588706E-4	7.773834284397177E-5
24-25	3.886917142198588E-5	0.0	0.0	0.0011272059712375906	7.773834284397177E-5
26-27	3.886917142198588E-5	0.0	0.0	0.002604234485273054	7.773834284397177E-5
28-29	3.886917142198588E-5	0.0	0.0	0.008181960584328027	7.773834284397177E-5
30-31	3.886917142198588E-5	0.0	0.0	0.031484028851808565	7.773834284397177E-5
32-33	3.886917142198588E-5	0.0	0.0	0.0861923876282537	7.773834284397177E-5
34-35	3.886917142198588E-5	1.943458571099294E-5	0.0	0.16280352450098787	7.773834284397177E-5
36-37	3.886917142198588E-5	3.886917142198588E-5	0.0	0.28838981736542424	7.773834284397177E-5
38-39	3.886917142198588E-5	3.886917142198588E-5	0.0	0.489945905774132	7.773834284397177E-5
40-41	3.886917142198588E-5	3.886917142198588E-5	0.0	0.7192157134067158	7.773834284397177E-5
42-43	3.886917142198588E-5	3.886917142198588E-5	0.0	0.958105640966241	7.773834284397177E-5
44-45	3.886917142198588E-5	3.886917142198588E-5	0.0	1.2569707000298904	9.71729285549647E-5
46-47	3.886917142198588E-5	3.886917142198588E-5	0.0	1.6096889960987013	1.3604209997695058E-4
48-49	3.886917142198588E-5	3.886917142198588E-5	0.0	1.9733100947513793	1.5547668568794353E-4
50-51	3.886917142198588E-5	3.886917142198588E-5	0.0	2.3528869882727825	1.5547668568794353E-4
52-53	3.886917142198588E-5	3.886917142198588E-5	0.0	2.7376529161790204	2.3321502853191527E-4
54-55	3.886917142198588E-5	3.886917142198588E-5	0.0	3.1785459276186065	2.3321502853191527E-4
56-57	3.886917142198588E-5	3.886917142198588E-5	0.0	3.6926490234315024	2.5264961424290825E-4
58-59	3.886917142198588E-5	3.886917142198588E-5	0.0	4.241831546452741	2.7208419995390117E-4
60-61	3.886917142198588E-5	3.886917142198588E-5	0.0	4.816259596312559	2.7208419995390117E-4
62-63	3.886917142198588E-5	3.886917142198588E-5	0.0	5.3930003618719855	2.7208419995390117E-4
64-65	3.886917142198588E-5	3.886917142198588E-5	0.0	6.020426526965682	2.7208419995390117E-4
66-67	3.886917142198588E-5	3.886917142198588E-5	0.0	6.749320664056472	2.7208419995390117E-4
68-69	3.886917142198588E-5	3.886917142198588E-5	0.0	7.543029144493424	2.7208419995390117E-4
70-71	3.886917142198588E-5	3.886917142198588E-5	0.0	8.363596222382967	2.7208419995390117E-4
72-73	3.886917142198588E-5	3.886917142198588E-5	0.0	9.183055528886985	2.7208419995390117E-4
74-75	3.886917142198588E-5	3.886917142198588E-5	0.0	10.031491802686093	2.915187856648941E-4
76-77	3.886917142198588E-5	3.886917142198588E-5	0.0	10.966334244556275	3.1095337137588706E-4
78-79	3.886917142198588E-5	3.886917142198588E-5	0.0	11.980178277341643	3.1095337137588706E-4
80-81	3.886917142198588E-5	3.886917142198588E-5	0.0	13.020084089565454	3.3038795708688E-4
82-83	3.886917142198588E-5	3.886917142198588E-5	0.0	14.067569390216551	3.498225427978729E-4
84-85	3.886917142198588E-5	3.886917142198588E-5	0.0	15.135869132164123	3.498225427978729E-4
86	3.886917142198588E-5	3.886917142198588E-5	0.0	15.959604047524559	3.498225427978729E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2475	0.0	42.9557	1
GTATCAA	4130	0.0	42.56938	1
TCAACGC	5350	0.0	32.565357	4
ATCAACG	5415	0.0	32.25912	3
CAACGCA	5585	0.0	31.113012	5
TATCAAC	5615	0.0	31.029037	2
AACGCAG	5865	0.0	29.779947	6
GTACATG	8635	0.0	26.169968	1
TACATGG	8810	0.0	24.616123	2
ACGCAGA	7030	0.0	24.584023	7
ACATGGG	9180	0.0	23.124073	3
CGCAGAG	7395	0.0	23.1222	8
CATGGGG	6375	0.0	20.56894	4
GCAGAGT	8340	0.0	19.403297	9
TAAGGCG	480	0.0	19.10358	5
TATACCG	240	2.0537482E-7	19.10358	5
GTGGTAT	1595	0.0	18.178745	1
GAGTACA	6600	0.0	17.781986	1
AGAGTAC	6905	0.0	17.659765	10-11
AGTACAT	6480	0.0	17.335068	2
>>END_MODULE